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  • www.sciencemag.org/content/354/6311/aaf2786/suppl/DC1

    Supplementary Materials for

    Systems-level analysis of mechanisms regulating yeast metabolic flux Sean R. Hackett, Vito R. T. Zanotelli, Wenxin Xu, Jonathan Goya, Junyoung O. Park,

    David H. Perlman, Patrick A. Gibney, David Botstein, John D. Storey, Joshua D. Rabinowitz*

    *Corresponding author. Email: [email protected]

    Published 28 October 2016, Science 354, aaf2786 (2016)

    DOI: 10.1126/science.aaf2786

    This PDF file includes:

    Figs. S1 to S15 Tables S1 to S6 Captions for Tables S7 to S9 References

    Other Supplementary Material for this manuscript includes the following: (available at www.sciencemag.org/content/354/6311/aaf2786/suppl/DC1)

    Tables S7 to S9

    http://www.sciencemag.org/content/%5bvol%5d/%5bissue%5d/%5bms.no.%5d/suppl/DC1 http://www.sciencemag.org/content/%5bvol%5d/%5bissue%5d/%5bms.no.%5d/suppl/DC1

  • Supplemental Tables

    Pathway R ea

    ct io

    ns w

    ith m

    ea su

    re d

    en zy

    m es

    To ta

    lp at

    hw ay

    re ac

    tio ns

    Fr ac

    tio n

    of re

    ac tio

    ns w

    ith m

    ea su

    re d

    en zy

    m es

    M ea

    su re

    d en

    zy m

    es

    To ta

    le nz

    ym es

    Fr ac

    tio n

    of m

    ea su

    re d

    en zy

    m es

    Glycolysis / Gluconeogenesis 16 16 100% 17 18 94% Citrate cycle (TCA cycle) 12 12 100% 12 12 100% Biosynthesis of amino acids 73 83 88% 63 75 84% Purine metabolism 37 52 71% 28 41 68% Pyrimidine metabolism 27 39 69% 22 31 71% All reactions 346 524 66% 304 510 60%

    Table S1: Proteomic coverage of enzymes in major metabolic pathways. For each pathway, we show both the fraction of reactions for which at least one isozyme was measured, as well as the total fraction of enzymes measured. Pathway annotations are based on KEGG.

    58

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    59

  • 1-by-1 testing of whether each metabolite as a regulator improves regulation-free model (based on fit only, no Bayesian analysis)

    Does not improve Signi

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