11

““Isolation and characterisation of Isolation and characterisation of Napthylisoquinolinealkaloids Napthylisoquinolinealkaloids

fromfrom Ancistrocladus cochinchinensis”Ancistrocladus cochinchinensis”

Suraya M. Vargas R.Suraya M. Vargas R.1.12.041.12.04

1

22

Occurence of AncistrocladaceaeOccurence of Ancistrocladaceae

2

33

Collection of Collection of The plant materialThe plant material

44

Dried material of Dried material of A.cochinchinensisA.cochinchinensis Extracted Extracted

with MeOHwith MeOH

EvaporationEvaporation

FiltrationFiltration

HPLCHPLCCleaning Cleaning

Extraction ProcedureExtraction Procedure

55

Using the „analytical triade“Using the „analytical triade“• UV spectrum: second max. is shifted

• Mass spectrum: m/z = 420

• NMR spectrum: six aromatic protons

new NIQ ?

HPLC-UV 216 nm

HPLC-UV 216 nm

66

Dried material of Dried material of A.cochinchinensisA.cochinchinensis Extracted Extracted

with MeOHwith MeOH

EvaporationEvaporation

FiltrationFiltration

Preparative Preparative HPLCHPLC

Separation funnelSeparation funnel

Isolation-ProcedureIsolation-Procedure

77

Structure elucidationStructure elucidation

Ancistrocladinium A

+

+

88

Cleaning our compoundCleaning our compound

Decomposition

In the NMR spectrum, the compound is not entirely clean!

TLC gave a good separation, we detected some alkaloids with Dragendorff´s Reagent which are also fluorescend.

99

Elucidation of Peak 7Elucidation of Peak 7HPLC-UV 216 nm

HPLC-UV 216 nm

working structure

1010

CD-SpectrumCD-Spectrum

1111

Prof. Prof. BringmannBringmann

All the rest of the groupAll the rest of the groupThank youThank you

Mexican GroupMexican Group

Inga KajahnInga Kajahn Torsten NollTorsten Noll

Prof. Prof. QuintanillaQuintanilla