sustainable control of grapevine trunk...
TRANSCRIPT
![Page 1: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/1.jpg)
ESF provides the COST Officethrough a European Commission contract
COST is supported by the EU Framework Programme
Sustainable control of Grapevine Trunk Diseases FA 1303 - ManaGTDs Start date: 22/10/2013 End date: 21/10/2017 Year: 2 FONTAINE Florence Chair University of Reims Champagne-Ardenne - FRANCE
![Page 2: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/2.jpg)
Website http://managtd.eu/
2
FA1303 News - Working groups – Partners – Meetings – Events – STMS - School
2/13/15, 10:10 AMNews
Page 1 sur 1http://managtd.eu/en/news/
manaGTD Sustainable control of grapevine trunk diseases (/)
You are here: Home (/en/) > News
Skip to content Go to menu
26
February 2015
Working Group 1 and WorkingGroup 2 meeting - Budapest,
Hungary: 26-27/02/2015(/en/news/working-group-1-and-working-group-2-meeting-budapest-hungary-26-27-02-2015.html)
Read more
Read more
Read more
Read more
News
Working Group 1 and Working Group 2 meeting - Budapest,Hungary: 26-27/02/2015
Local organizers: Dr Erzsebet Karaffa associate professor University of DebrecenFaculty of Agricultural and Food Science and Environmental Management Institute ofFood Science H-4032 DEBRECEN, Böszörményi út 138., Hungary Tel.: +36 52 508 444 /88067 ext E-mail: [email protected], [email protected]://portal.agr.unideb.hu ...
(/en/news/working-group-1-and-working-group-2-meeting-budapest-hungary-26-27-02-2015.html)
Call for proposals: "Search for applied solutions to controlgrapevine trunk diseases" - Funded by Jas HENNESSY & CoCall time schedule • December, 15th, 2014: call announcement;• February, 15th, 2015: deadline for submittingpre-proposals;• February, 15th - March, 15th 2015: evaluation of the pre-proposals;• March 2015: communicationto the applicants of the evaluation results and rating (A,B or C);• May , 15th 2015: deadline for submitting fullproposals;• May...(/en/news/call-for-proposals-search-for-applied-solutions-to-control-grapevine-trunk-diseases-funded-by-jas-hennessy-amp-co.html)
Disease cyclesDavid Gamaje and Philippe Larignon prepared a document including the graphical representation of the diseasecycle of some grapevine trunk disease agents. The document is attached below....(/en/news/disease-cycles.html)
Other Working Group meetingsWG3 (Host-pathogen and fungus-fungus competitive interactions) meetingAthens, Greece – 14-15 May 2015LocalOrganizers: Pr Epameinondas Paplomatas Agricultural University of Athenshttp://www.aua.gr/inde...(/en/news/other-working-group-meetings.html)
(http://ec.europa.eu/) COSTis supported by the EU RTDFramework Programme
(http://www.esf.org/) ESFprovides the COST Officethrough a European (http://www.cost.eu/) manaGTD is
supported by COST
Legal Notice
Accessibility
Sitemap
![Page 3: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/3.jpg)
Tuesday 12th, May
3
Athens, 12 -13 May 2015
09:00 Welcome to participants – local organizers 09:30 Florence Fontaine Synthesis of WGs1&2 meeting – Budapest – Feb2015
Plant pathogen interaction Chairman/ moderators Christophe Bertsch/Eckhard Thines
9:45 Jacques Alban France What can we learn from the cutting nodal model on Esca-associated fungi interactions with grapevine?”
10:45 Coffee break 11:00 Ana Cristina Esteves Portugal Enzymes involved in grapevine diseases 12:00 Rebecca Cobos Spain Secreted proteins from Diplodia seriata and their putative role in pathogenesis
13:00 Lunch break
![Page 4: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/4.jpg)
Tusday 12th, May
4
• Pathogen Infection • Chairman/ moderators Anna Andolfi/ Epaminondas Paplomatas
• 14:30 Dimitris Tsitsigiannis Greece Unravelling the Molecular Networks of the Plant Immune System
• • 15:30 Eliane Abou-Mansour • Switzerland Phytotoxins of Botryosphaeria associated with
grapevine • 16:30 Coffee break • • 17:00-18:30 Round table • • 19:30 Social dinner
![Page 5: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/5.jpg)
Wednesday, 13th morning
• • Plant defense Chairman/ moderators Florence Fontaine/ Sotiris
Tjamos • 09:00 Synthesis -Round table • 11:30 Conclusion Florence Fontaine • 12:00 End of the meeting Florence Fontaine – Workshop in Cognac
5
![Page 6: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/6.jpg)
Synthesis of WGs1&2 meeting, Budapest
• Pathogen characterization and detection James Woodhall
6
• What are the most important GTD species • Identify the reference isolates and mechanism of access • Standard test conditions to determine the
aggressiveness of GTD pathogens • Characterization of the GTD pathogens life cycle !
lecture to Cognac Workshop
![Page 7: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/7.jpg)
The trouble with Phomopsis viticola
7
Some uncertainty over which species/sequences
Potential community project/paper – with Pedro Crous?
Collection of grapevine Phomopsis isolates -Josep, Artur, Phillippe, Kalman, David E, David G, Ales, Erzsebet, Ferenc, Stefania & James
Determine which strain/species most important for grapevines
![Page 8: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/8.jpg)
8
• Reference collection of approx. 30 GTD isolates
Purpose: Standards for diagnostics & pathogenicity testing
30 isolates representing the most important species Where variability exists within species exists 2-3 isolates
80C agar plugs/glycerol method Collection distributed to key labs in several countries where -80C All isolates backed up at CBS and given CBS numbers
![Page 9: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/9.jpg)
9
Actions: Artur Alves to circulate protocol on -80C storage method
Determining which isolates and providing strains: Black foot pathogens: Cecilia/Anna? Esca/Petri: Josep, David G Cadophora: Josep Phomopsis: Chosen after community activity? Eutypa: David G, James (probably need 2-3 isolates) Botryosphaeria: Artur
Ideally isolates should have been used in publication, DNA barcoded and been tested for pathogenicity
James to circulate draft list to populate
• Reference collection of approx. 30 GTD isolates
![Page 10: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/10.jpg)
10
– Stipulating which specific conditions (temp, RH) may be too difficult could provide some general recommendations
– Olivier to look into host material what is available EU wide – maybe 4-5 recommended varieties/clones available.
– Specialists to review literature for specific methods for: -Esca/Petri & related species – David Gramaje -Illyoncectria – Cecilia/Anna? -Botryosphaeriaceae – Artur Alves -Eutypa- David Gramaje -Other species – James Woodhall
-Report back with recommended method for that group of pathogens
• Develop standard test conditions to determine the aggression of individual GTD
![Page 11: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/11.jpg)
11
• RT Pathogens Epidemiology L. Guérin-Dubrana and L. Mugnai
COST Objectives • 1- Surveys to assess the presence and
spread of the different GTDs in Europe ! questionnare in process!lecture to Cognac workshop
• 2- Multi site evaluation trials • 3- Alternative tools for spatial distribution
analysis • 4- Databases for prediction models
![Page 12: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/12.jpg)
12
• Multi site evaluation trials
a. To gather information on vineyards with historical data in the country from previous assesments
b. To detect in each country three reference viticulture areas with uniform productive/climatic conditions
c. For each area to select 1 vineyard of a susceptible international cultivar chosen on the base of the 1st level survey
d. In each of the 3 vineyards to select a GEO-REFERENCED plot for GTDs survey
- The same training system – Possibly of know rootstock
-12-18 yers old - 500 vines at least
![Page 13: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/13.jpg)
13
• The main diseases/symptoms to be surveyed will be: – Eutypa dieback + Botryosphaeria canker :
• Eutypa foliar symptom • Decline • Dead cordon
– GLSD (Esca): • foliar symptom • measles • canes/clusters wilting
– Apoplexy (halph or the whole plant) – Number of dead plant and presence of other diseases (viruses,
phytoplasmas, heavy pests attacks…)
We will not survey: Phomopsis – Black foot – Petri disease (0-7/8 year old)
• Multi site evaluation trials
![Page 14: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/14.jpg)
14
Inventory of microflora and identification of beneficial microorganisms
Patrice Rey
1. Study microbial communities colonizing wood tissues : plants sampled in different countries
2. Sampling of vines over time
3. Interactions between microorganisms
4. Wood tissues : a source of potential biocontrol agents
![Page 15: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/15.jpg)
15
To know the pathogens involved in GTDs but also to isolate BCAs specific to certain regions
Standardize the sampling method : " Compare the cultivars =
• Same international cultivars (Chardonnay, Cabernet) • Cultivar specifics to certain regions (Furmint to produce tokai,
Mavrud in Bulgaria) " Cultivars use to produce wine / table grapes (South of Italy) ? " Organic or conventional vineyard ? = conventional vineyard " Same training system (see RT2) ?
Rootstocks = International rootstocks : SO4 ? To select among the first 3 rootstocks
![Page 16: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/16.jpg)
16
Foliar GTDs-symptomatic / Asymptomatic : Use vineyards that are surveyed. Uproot grapevine : not a problem, even for healthy plants !
Age of plants: 15- 20 year-old plants
Sampling time : at the veraison (after the symptoms has appeared)
Which part of the plants have we to sample ? = at least 2 samples from the trunk (apparently healthy wood and the most representative necrosis)
Connect quantity of necroses / foliar symptoms / microflora
Study the microflora that colonise the rhizosphere ?
![Page 17: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/17.jpg)
17
Standardize the materials and methods :
• DNA/RNA extraction = verify the quality of DNA/RNA • Amplifications, primers, etc = to use the same standard
protocol (to be on the website) • Send the DNA/RNA sequences to one platform
• Which parts of plants are the more useful to sample : at least 2 samples in the trunk (apparently healthy wood and the most representative necrosis)
1. Study microbial communities colonizing wood tissues : plants sampled in different countries
2. Sampling of vines over time – standardize protocol: uproot vine
![Page 18: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/18.jpg)
18
3. Interactions between microorganisms
" Imaging analyses : to study interactions between the microflora (bacteria / fungi) with tools like DOPE-FISH
" Cultivable methods to isolate and use the microorganisms
" Screening of microorganisms: first in Petri dishes (include wood extracts in the agar?) then on plants ?
" Role of bacteria: they can contribute to the breakdown of wood or remove compounds that may be toxic to decay fungi. They predispose wood to fungal attack (according to the literature).
" Bacteria + pathogenic fungus, in certain cases = association of wrongdoers?
![Page 19: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/19.jpg)
19
4. Wood tissues : a source of potential biocontrol agents
" Trichoderma strains are used as BCAs (not always isolated from grapevines) :
• Hungary = many Trichoderma are detected in the wood tissues (3 Trichoderma species from Tokai region, they grow at 5 °C, even at 30 °C).
• 2 Trichoderma species (Remedier) registered in Italy, they are applied on pruning wounds.
• Esquive in France (to control Eutypa dieback and esca)
" Combine BCAs : For instance: bacteria on the pruning wounds or endophytic bacteria + BCA in the rhizosphere
" Impact of BCAs on wine quality ?
![Page 20: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/20.jpg)
Guidelines for reimbursement
20
![Page 21: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/21.jpg)
Some guidelines • BEFORE MEETING • Step 1, accept invitation • Step 2, reimbursement: yes
• AFTER MEETING • Step 3, Travel Reimbursement Request (TRR) • ASAP
21
9/12/14, 3:24 PMInvitation to COST Event
Page 1 sur 3https://e-services.cost.eu/participation/8aa8040e06d1c890596b868bea732d89/
e-COSTe-COSTInvitation to COST EventDr Florence Fontaine
Meeting Details
Meeting ID ECOST-MEETING-FA1303-250914-044181
Title MC and WG 4 meeting
Start Date 2014/09/25
End Date 2014/09/26
Location Poland, Warsaw, Poland
Step1/5 Invitation : Accepted
Step2/5 Reimbursement : Yes
If you decided to NOT submit a claim, please click the 'No' button above.
Step3/5
Travel information and Expenses
Please read carefully the Travel Reimbursement Request (TRR) Rules before completing your expenses.
Travel dates (Door to Door)
Travel Start Date 2014-09-25 Time
Travel End Date 2014-09-26 Time
Meeting Attendance
Check all the attended days according to the signed attendance list.
25 Sep 14
26 Sep 14
Accommodation Expenses i
Number of nights
Applicable Rate per Night EUR 120.00
Reimbursable amount EUR 0.00
Meals Expenses i
Number of meals
![Page 22: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/22.jpg)
Reimbursement procedure
22
• Keep your original invoices !
– Flight tickets and boarding passes
– Train tickets – Travel by car: proof of distance
which shows the traveled route (print-out from Google Maps)
– Local transport expenses: every tickets
– Parking tickets
After the meeting : • Complete your online
reimbursement request (= your invitation on e-COST)
• Detail each means of transportation (don’t put together the flight price and the airport shuttle price, for example)
• Upload all your supporting documents
• Send the signed Travel Reimbursement Request to:
Nathalie RAU – Violaine Béasse University of Reims Champagne Ardennes 9 Boulevard de la Paix CS0005 51724 REIMS - France
1 month to submit your travel reimbursement request on e-COST !
If you have a question, feel free to send it to us before submitting your
claim !
During the meeting : • Sign the attendance list • Sign your mission order
![Page 24: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/24.jpg)
Thank for your attention
24
![Page 25: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/25.jpg)
Year 2: Workshop – 4 WGs • 23 & 24 June, 2015 • Cognac, Fr • Collaboration with Hennessy
25
Paris
![Page 26: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/26.jpg)
26
Year 2: Workshop – 4 WGs • Number of participants: 85 • Invited speakers: 3, Kendra (USA),
François Halleen (SA), José Ramon (Canada)
• Duration: 2 days • Schedule: days 1 & 2, conferences • day 2-afternoon, trip & cellar • day 2-night, social diner
![Page 27: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/27.jpg)
• 4 sessions • 35 lectures • 13 posters • Need information for transfer:
• Angoulème railway • Bordeaux Airport
27
Workshop – GTDs: current state and future prospects
![Page 28: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/28.jpg)
Website - platform • the website managtd.eu hosts a document sharing platform • If you are interested, please follow this link:
http://managtd.eu/en/members/subscribe/ to register on the website.
• After our validation, you will be able to download documents in the Members Area.
• In "Edit my profile" you can also tick in which working group(s) you want to be registered.
• This part of the website is not open to the public. Only the registered members can access to the documents.The platform is for now divided between the 4 working groups of the COST Action.
• Email of Sandrine Plaud June 2nd, 2014
28
![Page 29: Sustainable control of Grapevine Trunk Diseasesmanagtd.eu/images/uploads/content/172/MeetingAthens_FA... · 2016. 3. 13. · 10:45 Coffee break 11:00 Ana Cristina Esteves Portugal](https://reader035.vdocument.in/reader035/viewer/2022071101/5fda5a48d7284f7ef63d9f5f/html5/thumbnails/29.jpg)
Thank for your attention
Thank Sotiris & Epaminondas for the organization Thank Eliane for the scientific programme
29