the characteristics of model organisms lab manual—2b
TRANSCRIPT
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The Characteristics of Model Organisms
Lab Manual—2b
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Plan for Lab
Tues. (1 hr early)—Duffy will start yeast broth
Tuesday—Finish Lecture, Parts I, II & III
Wednesday—FIRE, Part IV
Thursday—repeat Part IV/start write-up
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Model Organisms
Relatively easy to grow and maintain in a restricted space
Relatively easy to provide necessary nutrients for growth
Relatively short generation time (birthreproductionbirth)
Relatively well understood growth and development Closely resemble others organisms or systems
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Examples
Animal Kingdom—rats, mice (lots of offspring is a +)
Bacteria—Escherichia coli (E. coli)-prokaryotes
Fungal—Aspergillus (mold)--eukaryotes Yeast—Saccharomyces cerevisiae
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Lab Write-up
PurposeMaterialsSafety PrecautionsProceduresData Collection & Analysis (table 2.3—add 8C, only 2
trials to average)Conclusion (aka Data Analysis)
REE-results, evidence, explanationPE—possible errorPA—practical applications
Thinking Like a Biotechnician
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Background of Purpose
To grow and study an organism in alaboratory, one should know theenvironmental preferences of the species. Maintaining an organism at less than optimallight intensity, temperature, or oxygen level,from example, may put the organism understress, possibly affecting its growth or otherprocesses.
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Purpose
What are the temperature preferences shown by three model organisms (E. coli, Aspergillus niger and baker’s yeast) grown in a biotechnology lab?
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Prep. For Lab 2B
Month Prior Check supplies: bleach, petri dishes, LB base,
inoculating loops, 3 incubators, yeast, glucose, foil Order: E. coli, Aspergillus, Potato plates Week Prior Perez will prepare LB agar & broth Pour plate (2 per group20) Set incubators at 30, 37 and 42 C Scalpels from other room1 day prior make bacteria culture
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Pre-lab—Plate Pouring
Pour LB agar base plates (Luria-Bertani )E. Coli plate—use the LB agar with sterile
technique, use a 10ml pipette to transfer 10ml into a sterile plate
Yeast plate—use the LB* agar, sterile technique, transfer 10ml using a pippet
*we are not using Malt extract agar because we do not have it, but LB agar should have enough nutrients to grow yeast
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Each group does 1 plate of each organism at their assigned Temperature—wrap in foil for dark
1. room2. room3. 30C4. 30C5. 37C6. 37C7. 42C8. 42C9. 8C (instead of the suggested 4C)—please do 2
sets
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Part I Tips
Label the bottom Inoculating loop Triple Z streaking method Close lids with tape Make sure to use plate with LB agar
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Part II Tips
Each group gets one plate instead of a tube of potato dextrose agar
We are using a potato dextrose agar plate NOT tube
Cut aspergillus from stock plate—will be in the hood
Put the aspergillus (fungi) on the plate fungi side down to the agar and close the lid
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Part III Tips
Label the bottom Inoculating loop Triple Z streaking method Close lids with tape Make sure to use plate with LB agar
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Part IV—Data Collection & Analysis
Make sure to come in at FIRE on Wednesday to do a table like 2.3 (add space for 8C)
We will repeat the table again on Friday in class This will provide us with more information of what
happens over time as well as temperature The growth is exponential, but when nutrients run
out, they go into the death phrase (smelly!), we want to try to catch them at their peak
Use results from all the groups posted on the internet for you data
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Tutorials
Media Prep. (13) LB Agar and Broth (14) Sterile technique (15) Pouring Plates (16) Starting a Broth (18) Streak Colonies (17)