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The Two-Hybrid Method or Identifying and Analyzi rotein-Protein Interaction

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The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions. TAD. TAD. Sst2 interacts with Ste2 in a DEP-dependent manner in vivo. nucleus. Ub C-terminal hydrolase. NubG. HIS3. TAD. HIS3. TAD. HIS3. HIS3 encodes imidazoleglycerol-phosphate dehydratase - PowerPoint PPT Presentation

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Page 1: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions

The Two-Hybrid Methodfor Identifying and AnalyzingProtein-Protein Interactions

Page 2: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions
Page 3: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions
Page 4: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions
Page 5: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions
Page 6: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions
Page 7: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions

TAD

TAD

Page 8: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions
Page 9: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions
Page 10: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions
Page 11: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions

Ub C-terminal hydrolase

nucleus

Sst2 interacts with Ste2 in a DEP-dependent manner in vivo

NubG

Page 12: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions

HIS3

HIS3

HIS3

TAD

TAD

Page 13: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions

HIS3 encodes imidazoleglycerol-phosphate dehydratase[D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate hydro-lyase, EC 4.2.1.19]

His1 His4B His4A His6 His7 His3 His5 His2 His4C His4C

3-AT

H20

Page 14: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions

C

CH2—CH—COOH

NH2

L-histidine 3-aminotriazole

imidazole ring triazole ring

Page 15: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions
Page 16: The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions

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