Title: Identifying Nutrients: Title: Identifying Nutrients: Carbohydrates, Proteins, and Carbohydrates, Proteins, and

LipidsLipids

► Abstract: There are six essential nutrient Abstract: There are six essential nutrient groups that make up 96% of all living things. groups that make up 96% of all living things. Carbohydrates, Proteins and Lipids are three Carbohydrates, Proteins and Lipids are three of these categories that can be identified in of these categories that can be identified in food sources by testing for the presence food sources by testing for the presence with reagents such as Lugols, Benedicts, with reagents such as Lugols, Benedicts, Biurets and Sudan III. These chemicals Biurets and Sudan III. These chemicals changed colors to indicate the positive changed colors to indicate the positive presence of one of these nutrient groups.presence of one of these nutrient groups.

IntroductionIntroduction

►Background Information: Background Information:

Use your lab handoutUse your lab handout . The handout . The handout provided important information on the provided important information on the role of proteins, carbohydrates and role of proteins, carbohydrates and fats; the structure and function of each fats; the structure and function of each group and what food sources usually group and what food sources usually have these food groups. have these food groups.

ObjectiveObjective

►The objective of this study was to The objective of this study was to determine the presence of determine the presence of ______________, ________________, ______________, ________________, and___________________. If certain and___________________. If certain chemicals called indicators are added chemicals called indicators are added to food groups, then a color change to food groups, then a color change will occur and identify if one of the will occur and identify if one of the nutrients is present.nutrients is present.

MethodsMethods

►The method to performed the The method to performed the experiment is similar to one experiment is similar to one documented by (add internet URL) documented by (add internet URL) _________________________________________________________________________________________________________. Four test ___________________________. Four test tubes were filled with a small quantity tubes were filled with a small quantity of one food item each: of one food item each: ______________________________________________________________________________________________________________.________________________________.

► Also, potatoes and lard were placed in individual Also, potatoes and lard were placed in individual weigh boats and were also tested with the same weigh boats and were also tested with the same indicators. The weigh boats were used to avoid indicators. The weigh boats were used to avoid clogging test tubes. The test tubes were filled with clogging test tubes. The test tubes were filled with 3 droppers of one particular reagent.3 droppers of one particular reagent.

► The four tests were run using the following The four tests were run using the following reagents: Lugols, Benedicts, Biuret and Sudan III. reagents: Lugols, Benedicts, Biuret and Sudan III. Between each trial, test tubes were ____________ Between each trial, test tubes were ____________ and ____________, to avoid contamination. and ____________, to avoid contamination. Furthermore, when testing with Benedicts, the test Furthermore, when testing with Benedicts, the test tubes were placed in a water bath to break down tubes were placed in a water bath to break down the sugar bonds.the sugar bonds.

Results:Results:

►The following chart denotes the food The following chart denotes the food items and the reagent. A color change items and the reagent. A color change indicates the presence of Protein, indicates the presence of Protein, Carbohydrates and Fat. Carbohydrates and Fat.

► Insert table, chart or graph.Insert table, chart or graph.

DiscussionDiscussion

► The author noted that the potato responded The author noted that the potato responded to the reagent ____________ by changing from to the reagent ____________ by changing from yellow to ________________. This color change yellow to ________________. This color change tests positive for ____________. Therefore, tests positive for ____________. Therefore, potatoes must contain ________________. potatoes must contain ________________.

► Continue with the rest.Continue with the rest.► (Make sure you include errors, repeats, (Make sure you include errors, repeats,

weird observations and try to explain them…weird observations and try to explain them…did you forget to place test tubes in the did you forget to place test tubes in the water bath? Did you heat a test tube with water bath? Did you heat a test tube with the wrong reagent? Etc.)the wrong reagent? Etc.)

ConclusionConclusion

►The nutrient groups Proteins, The nutrient groups Proteins, Carbohydrates and Fats are essential Carbohydrates and Fats are essential life. A source of protein is found in life. A source of protein is found in __________. Biurets will change from __________. Biurets will change from ________to _________ indicating ________to _________ indicating proteins. Heat will speed up the proteins. Heat will speed up the reaction time. reaction time.

►Continue with your conclusions…Continue with your conclusions…

Reference and Reference and AcknowledgementAcknowledgement

►List the Internet URls,List the Internet URls,

►End with a quick few sentences End with a quick few sentences acknowledgement that your lab acknowledgement that your lab partner (s) provided. What test did partner (s) provided. What test did they perform?, did they assist?, lead, they perform?, did they assist?, lead, not do anything etc. not do anything etc.