to rerack the spectrum library, three programs will be run. specrerack1

7
ack the Spectrum Library, Three programs will be run. cReRack1 Moves 50 ul to Storage plate ABGene0781_384Storage c Aliquot Dispenses 10 ul from storage plate to three screening plates ABGene1055_384 Dispenses 3 ul into Plate with 72 ul of DMSO for diluted plate cRerack 70 ul Moves the remaining Chemical Library to Storage plate.

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To Rerack the Spectrum Library, Three programs will be run. SpecReRack1 Moves 50 ul to Storage plate ABGene0781_384Storage Spec Aliquot Dispenses 10 ul from storage plate to three screening plates ABGene1055_384 Dispenses 3 ul into Plate with 72 ul of DMSO for diluted plate - PowerPoint PPT Presentation

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Page 1: To Rerack the Spectrum Library, Three programs will be run. SpecReRack1

To Rerack the Spectrum Library, Three programs will be run.

1. SpecReRack1Moves 50 ul to Storage plateABGene0781_384Storage

1. Spec AliquotDispenses 10 ul from storage plate to three screening plates

ABGene1055_384Dispenses 3 ul into Plate with 72 ul of DMSO for diluted plate

1. SpecRerack 70 ul

Moves the remaining Chemical Library to Storage plate.

Page 2: To Rerack the Spectrum Library, Three programs will be run. SpecReRack1

Turn On instrument and hotel.Check Deck of instrument and remove all tip boxes and plates ect. Open Biomek SoftwareBe sure gripper UP

Home all axes.

Page 3: To Rerack the Spectrum Library, Three programs will be run. SpecReRack1

Thaw libraries (about 1 hour)Centrifuge briefly.Open SpecReRack1Set up Deck (red arrow)Hit start (Blue Arrow)

Page 4: To Rerack the Spectrum Library, Three programs will be run. SpecReRack1

Check deck againAll errors in deck set up will crash the robot. So check Again!Run program

Page 5: To Rerack the Spectrum Library, Three programs will be run. SpecReRack1

Close programRemove used tipsSpin Storage plate to remove bubblesOpen Spec AliquotSet up deck

Page 6: To Rerack the Spectrum Library, Three programs will be run. SpecReRack1

Run Spec Aliquots programCheck deck again when prompted

Page 7: To Rerack the Spectrum Library, Three programs will be run. SpecReRack1

Run SpecRerack 70 ul

Quick spin for all plates to put all chemicals at bottom of well.