tools & procedures
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Honors Biology Chapter 1 Section 4 SC B-1: Students will demonstrate an understanding of how scientific inquiry & technological design can be used to pose questions, seek answers, & develop solutions . Tools & Procedures. Essential Question:. - PowerPoint PPT PresentationTRANSCRIPT
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TOOLS &PROCEDURES
Honors BiologyChapter 1 Section 4
SC B-1: Students will demonstrate an understanding of how scientific inquiry & technological design can be used to pose
questions, seek answers, & develop solutions
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Essential Question:
How would you compare & contrast a light microscope & an electron microscope?
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Metric System used by most scientists based on certain physical
standards scaled on multiples of 10 used in SI
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Common Metric Unitslength
1 meter (m) = 100 centimeters(cm)
1 meter (m) = 1000 millimeters (mm)1000 meters(m) = 1 kilometer (km)
mass1 kilogram (kg) = 1000 grams (g)1 gram (g) = 1000 milligrams (mg)
1000 kilograms (kg) = 1 metric ton (t)
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more metric unitsvolume
1liter (L) = 1000milliliters( mL)1 liter (L) = 1000 cubic centimeters (cm3)
temperature0◦ C = freezing point of water100◦ C = boiling point of water
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Analyzing Biological Data Data Tables
create a table to record information measured in an experiment
name the table
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Biological Data new field of
BIOINFORMATICS
Computers used:To store dataTo make
sense of dataTo collect
data
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Microscopes devices that
produce magnified images of structures too small to see with naked eye
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Light Microscopes produce
images by focusing visible light rays through a specimen
limitations< 0.2 µ
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Electron Microscope produce
magnified images by focusing beams of electrons on specimen
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Compound Light Microscope up to 1000x
magnification light passes
through the specimen
uses 2 lenses specimen dead
or alive (if very small)
specimen may be stained to improve visulaizationStain entire cellStain selected
organelles or macromolecules
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Electron Microscopes Can produce
images 1000 times more detailed than light microscope
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Types of Electron Microscopes TEMs
(Transmission Electron Microscope)
beam of e- goes thru specimen
SEMs (Scanning Electron Microscopes)
beam scan across surface of specimen
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comparing Microscopes
Electron Compound Light
cannot use living cells
Very expensive higher resolution
Specimen could be living
Price not an issue Size limitations
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Cell Cultures Take small sample of cells &
place in petri dish with nutrients & allowed to divide.
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Cell Cultures might use the culture to:1. test cell responses under
controlled conditions2. study interactions between cells3. select certain cells for further
study
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HeLa Cells Taken from a patient with
cervical cancer in 1950’s Cells are still growing!
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Bacterial Cultures Made whenever something has
been tested for infection
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Cell Fractionation Separates cells into parts
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Lab Safety Rules Always follow read through lab
completely before starting Always follow instructions Ask if you do not understand
directions Always wash your hands at end
of lab