undergraduate students: 1.emmanuel garuz 2.fotis stringos 3.alicia racine 4.miguel cardenas...
TRANSCRIPT
PROJECT OBJECTIVES & OVERVIEW
PROJECT DESCRIPTION, EXPERIMENTS & RESULTS
HUMAN PRACTICE PROJECT
UNDERGRADUATE STUDENTS:
1. Emmanuel Garuz2. Fotis Stringos3. Alicia Racine4. Miguel Cardenas5. Salomon Mitre6. Valery Diaz 7. Naicolette A. Muñoz 8. Jose Ng9. Arturo Nieves10.Dukakis Tejada11.Kesia Barrows12.Michelle Camarena
INSTRUCTOR & DIRECTORGrimaldo Elías Ureña M.
HUMAN PRACTICE ADVISORLucia L. Palma
UTP-PANAMA TEAM 2011
• Agricultural, Food & Civil-
Environmental Engineering
• Mechanical and Material Sciences
Engineering
• Electrical & Computational Engineering
Engineering tools obtained from biological resources
SYNBIO ENGINEERING TOOL KIT
Design of powerful sensors
Improvement of organisms ability to survive under temperature changes
Focus on environmental and climatic issues.
TEAM MAIN OBJECTIVES
Applications
PROJECT OBJECTIVES & OVERVIEW
PROJECT DESCRIPTION, EXPERIMENTS & RESULTS
HUMAN PRACTICE PROJECT
“THE RENBO” (THERMOGENIC RESPONSE NUTRIENT BIOSENSOR)
• Nitrates biosensor
(BioBrick
BBa_K381001)
• AOX periplasmatic
heat generator
(BioBrick
BBa_K410000)
PROJECT DESCRIPTION
Make Competent Cells using JM109Test our Competent CellsGet Familiarized with Protocols • New England Bio Labs• Open Wet Ware
• Reagents and buffer preparation
• Transformation• Mini Prep• Electrophoresis• Digestion • Ligation
LAB WORK
Test operation using 40mM KNO₃(if needed) under different:
• Substrates:• LB• Minimal essential medium• Saline solution
• Temperatures:• 8°C• 23°C• 37°C
Improve the characterization of pre-existing BioBricks
EXPERIMENT DESIGN OBJECTIVES
LB MEM SS
0.015
0.021
0.0050.006
0.004
0.014
0.000
0.006
OPTICAL DENSITY AT 8°C AND 420nm
BBa_K381001 BBa_K410000 BBa_K672000
LB MEM SS
0.599
0.341
0.010
0.258 0.275
0.015
0.138
0.0210.002
OPTICAL DENSITY AT 23°C AND 420nm
BBa_K381001 BBa_K410000 BBa_K672000
LB MEM SS
0.961
0.351
0.006
0.624
0.028
-0.001
0.742
0.006 0.003
OPTICAL DENSITY AT 37°C AND 420nm
BBa_K381001 BBa_K410000 BBa_K672000
LB MEM
0.225
0.005
Optical Density at 23°C and 534nmBBa_K381001
LB MEM
0.483
0.136
Optical Density at 37°C and 534nm
BBa_K381001
CONCLUSION
Determining the experimental conditions for optimal results for The Renbo.
• Best growth in LB @23°C
Improving characterization of pre-existing BioBricks
• New results for BBa_K381001 and BBa_k410000 in different substrates and temperatures.
• The PyeaR promoter showed a better activity over the HybB promoter.
• CspA promoter
(BBa_K328001)
• OmpA + AoX enzyme
• Nitrates biosensor
(BBa_K381001)
FUTURE DEVELOPMENTS
PROJECT OBJECTIVES & OVERVIEW
PROJECT DESCRIPTION, EXPERIMENTS & RESULTS
HUMAN PRACTICE PROJECT
1. How to introduce Synthetic Biology into our society.
2. Be able to find all the availability of institutions and the clear regulatory frame to develop our project.
1. The development of an outreach campaign about synthetic biology basic topics for every kind of public.
2. The development of a interactive software introducing the basics of synthetic biology and the iGEM competition.
3. The development of a guidance software that assists the user in the execution of projects related with synthetic biology.
PROBLEMS
OBJECTIVES
Synthetic Biology Tour with brochures, visuals, speeches and surveys.
OUTREACH
SURVEY’S RESULTS
Our team is also part of a new project called “Community Bricks”, a post iGEM group, that will be released this year. We are also participating in the set up of the Alumni iGEM.
SB UTP 1.0 is an interactive and educational program that introduces Synthetic Biology and iGEM to the users.
This software is available on Visual Basic 2010.This software is available on Visual Basic 2010.
SB UTP 1.0
SB UTP PROJECT 1.0 is a guidance program that helps groups of people with the process of implementing a Synthetic Biology project in their country.
SB UTP PROJECT 1.0
Our future objective for this software is that it will end as a data base, so that teams of any part of the world could use it and also add more information.
This software is available.
At the end of every question of the program, you will find a website or a paper as reference about the topic.
We exhort all of the future teams to complement this software.
SPONSORS
ASSOCIATE COLLABORATER