university of california san francisco curriculum vitae · 2005 dean’s prize for research, ucsf...

Eric J. Huang Prepared 6-3-14

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University of California San Francisco

CURRICULUM VITAE

Name: Eric J. Huang

Position: Professor, Step 2 (Ladder Rank) Department of Pathology School of Medicine Member Program in Biological Sciences (PIBS), UCSF Neuroscience Graduate Program, UCSF Biomedical Sciences (BMS) Graduate Program, UCSF Developmental Stem Cell Biology Graduate Program, UCSF

Staff Pathologist Pathology Service 113B San Francisco VA Medical Center

Address: HSW450C 505 Parnassus Avenue San Francisco, CA 94143 Voice: 415-476-8525 Fax: 415-750-6947 E-mail: [email protected]

EDUCATION/TRAINING: 1979-86 National Taiwan University, Taipei, Taiwan M.D. Medicine 1988-92 Cornell University, New York, NY Ph.D. Molecular Biology 1993-95 University of California San Francisco Residency Pathology 1995-97 University of California San Francisco Fellowship Pathology 1997-99 Howard Hughes Medical Institute Res Assoc Neuroscience

PRINCIPAL POSITIONS HELD: 1997-99 University of California San Francisco Clinical Instructor Pathology 2000-2005 University of California San Francisco Assistant Professor in Res Pathology 2005-2009 University of California San Francisco Associate Professor in Res Pathology 2009-present University of California San Francisco Professor Pathology

ANCILLARY POSITIONS HELD CONCURRENTLY:

2000-present San Francisco VA Medical Center Staff Pathologist 2001-present Moffitt-Long Hospital Attending Neuropathologist 2001-present BMS Graduate Program, UCSF Member


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2005-present Programs in Biological Sciences (PIBS) & Member Neuroscience, UCSF 2005-present Institute of Neurodegenerative Diseases, UCSF Member 2005-present Alzheimer’s Disease Research Center (ADRC), UCSF Co-Director (Brain Bank) 2007-2009 San Francisco VAMC, Pathology Service Acting Chief 2009-present Pediatric Neuropathology Research Lab, UCSF Director 2010-present Developmental Stem Cell Biology Graduate Program, Member UCSF 2010-present Brain Pathology, Editorial Board Member 2012-present Journal of Neuroscience, Editorial Board Associate Editor

HONORS AND AWARDS:

1990-91 Frank Lappin Horsfall, Jr. Fellowship Sloan-Kettering Cancer Center 1991 Vincent du Vigneaud Award of Excellence Cornell University 1997-99 Postdoctoral Fellowship for Physicians Howard Hughes Medical Institute 1998 Weil Award for the best paper in AANP Annual Meeting Experimental Neuropathology 1999 Advanced Career Development Award Dept of Veterans Affairs 2000 Young Investigator Award NCIRE & SFVAMC 2000 Merit Award Dept of Veterans Affairs 2000-05 Presidential Early Career Award for Dept of Veterans Affairs Scientists and Engineers (PECASE) 2002-07 Independent Scientist Award NINDS, NIH 2004 Weil Award AANP Annual Meeting 2005 Dean’s Prize for Research, UCSF SOM Medical Student Mentored: Stephen Ku 2007-08 Summer Student Research Fellowship Students Mentored: Ben Crittenden

Parkinson Disease Foundation (2007); Katherine Muller (2008) 2009-14 Mid-career Investigator Award NCRR/NIH 2012 Irene & Eric Simon Brain Research Student Mentored: Hansen Lui Foundation Summer Fellowship 2012-14 Elected Vice Chair (2012) & Chair (2014) Gordon Research Conference, Molecular

& Cellular Neurobiology, Hong Kong 2013 Member, Study Committee on Sports Institute of Medicine, National Academy -related Concussion in Youths of Sciences

KEYWORDS/AREAS OF INTEREST:

Neurobiology, Development, Neurotrophins, Transcription factor, Gene expression, Tyrosine kinase receptors, Signal transduction, Neurodegenerative disorders, Parkinson’s disease, Dopaminergic neurons

PROFESSIONAL ACTIVITIES

CLINICAL


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Attending Pathologist, Pathology Service, San Francisco VAMC: My clinical responsibilities at the VAMC include attending the autopsy service, all neuropathology consultation and sign-out and teaching of rotation pathology residents. I have been in this position for the past 10 years. Attending Neuropathologist, Dept of Pathology, Neuropathology Division, UCSF: I attend 5 months of the year of autopsy neuropathology service at the Moffitt Hospital, supervising 12-15 medical students, post-sophomore fellows, residents and fellows. I have been in this position for the past 10 years.

PROFESSIONAL ORGANIZATIONS: 1995-present American Association of Neuropathologists 2000-present American Society of Cell Biology 2000-present American Association for the Advancement of Science 2002-present Society of Neuroscience

LICENSES, CERTIFICATION: 1995 Medical License, California 1997 Certified, American Board of Pathology (Anatomic Pathology and Neuropathology)

SERVICE TO PROFESSIONAL PUBLICATIONS:

2000 J of Neurosci, J of Cell Biology Ad hoc reviewer 2001 J of Cell Biology, Neuroscience, FEBS letters, Neurocase Ad hoc reviewer 2002 Trends in Neuroscience Ad hoc reviewer 2003 Experimental Neurology Ad hoc reviewer 2004 J of Cell Biology, Neurocase, J of Comp Neurol, Am J Pathol Ad hoc reviewer 2005 Exp Neurol, Brain Res, Neuroscience, Cell & Mol Neurobiol, Ad hoc reviewer Am J Pathol 2006 Am J Pathol, Development, Brain/Dev/Evolution, J Neurosci Ad hoc reviewer 2007 PLoS Biology, J Cell Biology, J Neurochem, Dev Neurobiol, Ad hoc reviewer Aging Cell, Development, J Neuropath Exp Neurol, FEBS Letters 2008 Dev Neurobiol, J Neuropath Exp Neurol, European J Cancer, Ad hoc reviewer Neuroscience, Development, J Neurosci 2009 Brain Pathology, Development, Developmental Dynamics, Ad hoc reviewer J Neuropath Exp Neurol, J Neuroscience, Mol Cell Biol 2010 J of Cell Biology, Brain Pathology, British J Cancer, J Neurosci Ad hoc reviewer J Neuropath Exp Neurol 2011 J Neurochem, J Neurosci, J Neuropath Exp Neurol, Dev Neurobiol, Ad hoc reviewer Brain Pathology, Development, Annals of Neurology, Lancet Neurology Neural Development, Brain Research

INVITED SEMINARS, LECTURES AND PRESENTATIONS:

Regional

2001 Research Seminar Series, San Francisco VAMC 2002 GRECC, Palo Alto VA Medical Center 2002 BMS Graduate Program, Faculty Talk, UCSF


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2004 BMS Graduate Program, Faculty Talk, UCSF 2004 Society of Chinese Bioscientists in America (SCBA), Northern California Chapter, Annual

Meeting (invited speaker and session moderator) 2004 Speaker, Neuroscience Session, BMS Graduate Program Retreat, UCSF 2005 Research Seminar, Palo Alto VA Medical Center 2005 Research Seminar, San Francisco VA Medical Center 2005 Speaker, Program of Neuroscience Asilomar Retreat, UCSF 2006 Speaker, Program of Neuroscience Asilomar Retreat, UCSF 2006 SCBA, Northern California Chapter, Annual Meeting, Vice Chair 2007 Research seminar, Parkinson’s Institute, Sunnyvale, CA 2007 Seminar, City College of San Francisco, San Francisco, CA 2008 Faculty Lunch Seminar, Biomedical Sciences (BMS) Graduate Program, UCSF 2008 SCBA, Northern California Chapter, Annual Meeting, Chair 2009 Session Chair, Program of Neuroscience Asilomar Retreat, UCSF 2011 Speaker, Biomedical Sciences (BMS) Graduate Program Retreat, UCSF 2011 Faculty Lunch Seminar, Biomedical Sciences (BMS) Graduate Program, UCSF 2011 Session Chair, Program of Neuroscience Asilomar Retreat, UCSF 2012 Neuroscience Breakfast, UCSF 2012 Prion Review Meeting, UCSF 2013 Research Seminar, San Francisco VAMC

National

2000 Dept of Pathology, Baylor College of Medicine 2000 Dept of Pathology, University of Washington 2001 Research Seminar for PECASE awardees, Dept of Veterans Affairs Central Office,

Washington, DC 2001 Research Seminar, National Meeting of ACOS of Research, Dept of Veterans Affairs,

Albuquerque, NM 2001 American Association of Neuropathologists (invited speaker, short course) 2001 American Association of Neuropathologists (platform presentation) 2002 Dept of Pathology & Neurobiology, St. Jude’s Children Research Hospital 2003 Dept of Pathology, University of Alabama Birmingham 2004 Speaker, the Amyotrophic Lateral Sclerosis Association Meeting, Boston, MA 2005 Speaker, Short talk session, Gordon Research Conference on Neurotrophins, Newport, RI 2005 Session speaker, Annual Meeting, Society of Neuroscience, Washington, DC 2005 Research seminar, National Cancer Institute, National Institutes of Health 2006 Research seminar, MIND Institute, UC Davis, Davis, CA 2006 Grand Round, Dept of Pathology & Lab Medicine, ULCA, Los Angeles, CA 2006 Grand Round, Dept of Neurology, UCLA, Los Angeles, CA 2006 Research seminar, Dept of Pathology & Lab Medicine, University of Pennsylvania,

Philadelphia, PA 2007 Research seminar, Dept of Pathology, Yale University, New Haven, CT 2008 Research seminar, Dept of Pathology & Lab Medicine, UC Irvine 2008 Session Chair, Neurogeneration Platform Presentation, Annual Meeting of AANP, San

Diego, CA 2008 Research Seminar, Genentech, Inc., South San Francisco, CA 2009 Short Talk/Hot Topics, Gordon Research Conference on Neurotrophic Factors, Newport, RI


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2009 Visiting Neuropathologist, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA

2011 Research Seminar, Dept of Pathology, Columbia University, NY 2011 Speaker, RNA Binding Proteins in Neurological Diseases, SfN Satellite Symposium,

Washington, DC 2014 Seminar, Dept of Pathology, Northwestern University, Chicago, IL 2014 Minisymposium, “Neurodevelopment and Disease”, American Society for Investigative

Pathology, San Diego, CA 2014 UC Neurotrauma Meeting, Carmel, CA

International

2005 Research Seminar, School of Pharmacy, China Medical University, Taiwan 2005 Presentation, The Keystone Symposium on Stem Cells, Senescence and Cancer, Singapore 2006 Visiting Scholar, National Cheng Kung University, Tainan, Taiwan 2006 Research Seminar, Stem Cell Program, Institute of Biomedical Sciences, Academia Sinica,

Taiwan 2006 Speaker, NGF 2006, Hot Topics Section, Lyon, France 2006 Research Seminar, Dept of Biology, Ecole Normale Superieure, Paris, France 2006 Speaker, Short Talk Session, Gordon Conference on Molecular and Cellular Neurobiology,

Hong Kong 2006 Session speaker, 11

th SCBA International Meeting, San Francisco, CA, USA

2006 Session Co-chair, Animal Models, XVI International Congress of Neuropathology, San Francisco, CA, USA

2007 Neurology Grand Round, National Taiwan University Hospital, Taipei, Taiwan 2007 Research Seminar, Institute of Pharmacology, National Taiwan University College of

Medicine, Taipei, Taiwan 2007 Research Seminar, Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan 2008 Research Seminar, National Health Research Institute, Taipei, Taiwan 2008 Invited Speaker, Session on “The Dopaminergic Neuron – From Specification to Treatment

of Parkinson’s Disease”, 17th

Biennial Meeting, International Society for Developmental Neuroscience (ISDN), Asilomar, CA

2008 Session Chair on “Neuronal Development”, Gordon Research Conference on Molecular and Cellular Neurobiology, Hong Kong

2008 Research Seminar, Riken Center for Developmental Biology, Kobe, Japan 2008 Invited Speaker, International Symposium, Kanazawa University Center of Excellence

(COE) on the Innovative Brain Science, Kanazawa, Japan 2010 Session Chair on “Molecular & Cellular Basis of Behavior”, Gordon Research Conference

on Molecular and Cellular Neurobiology, Hong Kong 2010 8

th International Stem Cell School of Regenerative Medicine, Karolinska Institute,

Stockholm, Sweden 2012 Speaker, Gordon Research Conference on Molecular and Cellular Neurobiology, Hong

Kong 2013 Speaker, Keystone Symposium, New Frontiers on Neurodegenerative Research, Santa Fe,

NM 2013 Speaker, Gordon Research Conference on Dendrites: Molecules, Structure and Functions,

Les Diablerets, Switzerland 2014 Chair, Gordon Research Conference on Molecular and Cellular Neurobiology, Hong Kong,

China


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OTHER PRESENTATION AT SCIENTIFIC MEETINGS:

1992,1996 Mouse Molecular Genetics (poster session) 1995 United States and Canadian Association of Pathologists (platform presentation) 1996 American Association of Neuropathologists, annual meeting (poster session) 1998 American Association of Neuropathologists, annual meeting (platform) 1998 Keystone Symposium on Vertebrate Development (poster session) 2001 American Association of Neuropathologists (poster presentation) 2001 American Society of Cell Biology, Annual Meeting (poster) 2002 Keystone Symposium on Development of spinal cord and neural crest (poster) 2002-2005 American Society of Cell Biology, Annual Meeting (poster) 2003-2009 Gordon Research Conference on Neurotrophins, Newport, RI (posters/short talks)

2006, 2008 Keystone Symposium on Wnt/-catenin Signaling (poster) 2006-2010 Gordon Research Conference on Molecular & Cellular Neurobiology, Hong Kong

(posters/short talks) 2003-2010 Society of Neuroscience, Annual Meeting (poster presentations)

GOVERNMENT AND OTHER PROFESSIONAL SERVICE:

2004 National Science Foundation Ad hoc reviewer 2004, 2007 The Wellcome Trust (Neuroscience/Mental Health Section) Ad hoc reviewer 2005-07 NIH, Neurogenesis and Cell Fate Study Section Ad hoc reviewer 2006 NIH, Cell Death in Neurodegeneration Study Section Ad hoc reviewer 2009 NIH, ARRA Challenge Grant Study Section Ad hoc reviewer 2007-2011 NIH, Neurogenesis and Cell Fate Study Section Member 2010 Barrow Neurological Institute External grant reviewer 2010 VA Merit Review, RRD6 (Aging & Neurodegeneration) Ad hoc reviewer 2010 MRC, National Institute for Medical Research, UK Ad hoc reviewer 2010 Parkinson’s Disease Foundation, UK & Michael J. Fox Ad hoc reviewer Foundation for Parkinson’s Research

UNIVERSITY AND PUBLIC SERVICE:

UNIVERSITY SERVICE

UCSF campus-wide 2002-2007 Co-organizer, Molecular Basis of Neurological Diseases Seminar Series, Dept of

Pathology 2003-2007 Biosafety Committee, representative member from VA campus 2005-now Admission Committee (panel 1), School of Medicine, UCSF SFVAMC campus 2000-now Infectious Disease Control Committee 2003-2004 Career Development Grant Writing Seminar Co-instructor


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2003-2010 VA Research Seminar Co-organizer 2005-2010 IACUC Animal Studies Subcommittee, SFVAMC 2009-now Medical Executive Committee, SFVAMC

COMMUNITY SERVICE

2000-now UCSF-High School Teachers Science Education Partnership Program (SEP) (My lab has been an active participant of this successful program that provides hands-on

experience for talented high school students to the biomedical research. All graduates from my lab subsequently were interested in a career in science and were accepted to outstanding colleges, such as UC Berkeley, UCLA, UCSD and University of Chicago.)

TEACHING AND MENTORING: Qtr. Acad. Yr. Course No. & Title Nature of contribution Class size S 1995-96 Pathology 102 Laboratory instructor 30-40 S 1996-97 Pathology 102 Laboratory instructor 30-40 S 1997-98 Pathology 102 Laboratory instructor 30-40 Pre-F 1997 Pathology 101 Small group leader 12 Pre-F 1999 Pathology 101 Small group leader 12 S 1999-2000 Pathology 102 Laboratory instructor 30-40 F 2000 Tissue & organ biology Laboratory instructor 20 S 2000-01 Pathology 102 Laboratory instructor 30-40 S 2001-04 BMS program Neuroscience Laboratory instructor 15-25 F 2002-now Neuropathology small group Small group leader 10-13 S 2004-now BMS program 225A Lecturer 15-25 S 2006-now AIMS Small Group Small group leader 6 F 2008-now NS205A Lecturer 20-25 S 2008-now Mini-course/NS Program Organizer 8 W 2009-now NS201 (co-organizer) Mol/Cell/Dev Neurosci 15

(1) PREDOCTORAL STUDENTS SUPERVISED OR MENTORED:

Dates Name Program or school Role Current Position

2000-03 Esther Luo BS, Scripps College

Supervisor Critical Care Fellow, Mass General Hospital

2001-02 Nazzy Pakpour BS, UC Davis Supervisor UPenn

2001-03 Esther Moy BS, UC Berkeley Supervised summer work

M.D., Oregon Health Sciences University

2001-02 Stephen Ku Stanford Univ. (Jr.) Supervised summer work

M.D., UCSF School of Medicine

2003-04 Gene Ma Stanford Univ. (Fr.) Supervised summer work

MS4, Stanford University Medical School

2005-2006

Siu-Wah (Michael) Tang

UC Berkeley Supervisor Ph.D. candidate, Dept of Bioengineering,

University of Pittsburgh


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2006-2008

Sidney Hsin-Kuang Hsieh

B.S./M.S., McGill University

Supervisor

SRA, Genentech Inc.

2007 Ben Crittenden Cambridge University, UK

Mentor for Summer Student Fellowship

(Parkinson’s Disease Foundation)

PhD student, Cambridge University, UK

2008 Katherine Muller Oberlin College Mentor for Summer Student Fellowship

(Parkinson’s Disease Foundation)

Senior, Oberlin College

2008 & 2009

University of Chicago

Mentor for Summer Research

Senior, University of Chicago

2010 UC Berkeley Mentor for Summer Student Fellowship

(SUNY Alumni Fellowship)

Medical Student, State University of New York

(SUNY)

2011 Caitlin Carlton Oberlin College Mentor for Summer Research

Senior, Oberlin College

2011 Hansen Lui UCLA Mentor for UCSF-Amgen

Fellowship

Senior, UCLA

2011 Vivian Tang UC Berkeley Mentor for Summer Research

Senior, UC Berkeley

2011 Christina Doan University of Nevada, Reno

Mentor for Summer Research

Medical student, Univ of Nevada

(2) POSTDOCTORAL FELLOWS SUPERVISED OR MENTORED:

Dates Name Program or

school Role Current Position

2002-2005 Amanda Wiggins

Ph.D., University of Melbourne,

Australia

Research Supervisor Ministry of Research, Science and Technology,

New Zealand

2002-2006 Guangwei Wei Ph.D., Fudan University, China

Research Supervisor Professor, Shang-Dong University

2003-2005 Vanee Pho Ph.D., Boston University

Research Supervisor Career Scientist, Applied Biosystems,

Foster City, CA

2003-2005 Nako Ishikura M.D., Yamanashi Medical

University, Japan

Research co-supervisor (w/ Dr.

DeArmond)

Psychiatrist, Takatsuki Hospital,

Tokyo, Japan

2003-2006 Jared Clever Ph.D., UCLA Research co-supervisor (w/ Dr.

DeArmond)

Assistant Research Scientist, Vascular

Surgery, UCSF

2003-2006 Rebecca Laposa

Ph.D., University of Toronto


Cleaver)

Assistant Professor, Pharmacology,

University of Toronto

2004-now Jiasheng Zhang

Ph.D., Kanazawa University, Japan

Research Supervisor Postdoc fellow, UCSF & VAMC


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2005-now Mianzhi Tang Ph.D., Chinese Academy of

Science, Shanghai

Research Supervisor Assistant Research Neuropathologist,

UCSF

2006-2008 Christine Hsieh

Ph.D., Stanford University


Seaman)

Career Development Awardee, SFVAMC

2007-now Yulei Shang Ph.D., Zhejian University


2008-now Sebum Lee Ph.D., University of North Texas


2008-2009 Han S. Lee M.D., Ph.D., University of

Cincinnati

Research Supervisor Assistant Clinical Professor, UCSF

2008-2010 Ingrid Revet Ph.D. Research co-supervisor (w/ Dr. Cleaver)

Clinical Pathology, The Netherlands

2009-now Haiyan Qiu Ph.D., ION, Chinese Academy of

Sciences, Shanghai


2010-now Yonghua Pan Ph.D., Washington University, St. Louis

Research Supervisor Industry

(3) GRADUATE STUDENT ORAL/THESIS COMMITTEE/LAB ROTATION:

Dates Name Program Role Current Position

2005 Suzanne Angeli BMS Program, UCSF

Qualifying Exam committee member

Graduate student, Marc Diamond Lab

2005 Derek Southwell Neuroscience Program, UCSF


Graduate student, Arturo Alvarez-Buylla Lab

2006 Elizabeth Szyleyko

BMS Program, UCSF


Graduate student, Jim McKerrow Lab

2008 Alex Loucks Neuroscience Program, UCSF


Graduate student, of Katerina Akkasoglou Lab

2008 Erica Korb Neuroscience Program, UCSF


Graduate student, Steve Finkbeiner Lab

2008 Sarah Fischbach Neuroscience Program, UCSF


Graduate student, Antonello Bonci Lab

2009 John Silberesis Neuroscience Program, UCSF


Graduate student, David Rowitch Lab

2009 Karen Ring BMS Program, UCSF


Graduate student, Yadong Huang Lab

2009 Sachi Jain BMS Program, UCSF


Graduate student, Yadong Huang Lab

2009-now Sarah Xinwei Luo

Neuroscience Program, UCSF

Thesis Advisor UCSF NS graduate student

2008-now Nicole Reyes BMS Program, UCSF

Thesis project co-sponsor

Graduate student, Scott Oakes Lab


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2009-now Lauren Herl BMS Program, UCSF

Ph.D. Thesis Committee Member

Graduate student, Bob Farese Lab

2010 Hengameh Zahed

MSTP Program, UCSF


Graduate student, Steve Finkbeiner Lab

2011 Cheuk Ka Tong Neuroscience Program, UCSF

Qualifying Exam committee member (Chair)

Graduate student, Arturo Alvarez-Buylla Lab

(4) JUNIOR FACULTY MENTORED:

Dates Name Program/Dept Role Current Position

2006 Bonita Lippman-Hoskins

Dept of Growth & Development,

Division of Orthodontics, UCSF

BMS 225A Oral Exam mentor

Asst Clinical Professor

2008-2009 Yasunori Miyamoto

Dept of Biology, Ochanomizu

University, Tokyo, Japan

Host of sabbatical leave

Associate Professor

INFORMAL TEACHING:

2000-now Attending neuropathologist, SF VAMC (weekly conference with 3 residents at brain

cutting conference and at the microscope, 12 months per year) 2000-now Attending neuropathologist, UCSF Moffitt-Long Hospital (weekly conference with 5

residents, fellows and medical students at brain cutting conference and at the microscope, 9 months per year)

SUMMARY OF TRACHING HOURS

2001-02 400 total hours of teaching (including preparation) Formal class of course teaching hours: 30 hours Informal teaching hours: 300 hours Mentoring hours: 70 hours 2002-now 600 total hours of teaching (including preparation) Formal class of course teaching hours: 30 hours Informal teaching hours: 400 hours Mentoring hours: 170 hours

SUMMARY OF TEACHING RESPONSIBILITIES

My teaching efforts have been primarily in the School of Medicine and the Graduate Division at

UCSF. In addition, in my capacity as an attending neuropathologist at the UCSF Medical Center, San Francisco General Hospital and the San Francisco VA Medical Center, I provide education to residents and fellows from the Depts of Pathology and Neurology. The followings are more detailed descriptions to my teaching responsibilities.


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Teaching in the UCSF School of Medicine. For the past 10 years, I had served as an instructor in Pathology 102, and, beginning in 2002, have been a Small Group Leader in Basic Pathology Segment of Pathology 101 and in Neuropathology Small Group for Brain, Mind and Behavior. As a small group leader, I used several different teaching modalities to introduce the general concepts of pathology to second-year medical students. I used multiheaded microscope, projection device and autopsy cases as tools to expose the students to fascinating images of pathological processes. Meanwhile, I also encouraged the students to think about pathology in the bigger context of human diseases and to establish a rigorous thinking process when faced with live cases. This experience as a small group leader has been extremely rewarding and the feedback from the students has been excellent.

Teaching in the UCSF Graduate Division. As a member of the UCSF BMS Graduate Program, I have participated in the laboratory of Tissue and Organ Biology course and served as the coordinator for the neurobiology section. My roles in these laboratory sessions are to introduce the graduate students to the general field of neuroscience and to provide them with hands-on experience in neuroscience research and diseases involving human brain. Beginning in 2005, I also participate in the lecture series on Stem Cell Biology. In 2005, I became a member of the Neuroscience Program and have participated in the interview of applicants and qualifying exams for current students. For the Spring 2008 semester, I am the organizer of a mini-course on “The Dopaminergic System”. This mini-course has become an integral part of the neuroscience curriculum for second year graduate students. Beginning in 2009, I become a member of the organizing committee for NS201, the neuroscience core curriculum for the first year graduate students. The part of NS201 curriculum that I help organize is on the molecular, cellular and developmental neuroscience. This part of the NS201 runs for about 2 and a half months, from November to January every year. My responsibility include recruiting faculty for the lectures, designing problem sets for discussion, monitoring progress during classes, and grading for final exam.

Teaching in the UCSF Medical Center and Affiliated Hopsitals. As a neuropathologist, I have sign-out responsibilities with residents in autopsy pathology and neuropathology, both at the San Francisco VA Medical Center, San Francisco General Hospital, and at UCSF Moffitt Hosptial. Starting in July 2002, I have been heavily involved in autopsy brain sign-out (9 months per year) at UCSF Moffitt Hospital. During these occasions and other formal sessions, I provide teaching materials to introduce the residents to the general field of neuropathology. Furthermore, I participate in didactic lectures for medical student and residents on selected topics in developmental neuropathology.

Teaching for the Science and Education Partnership Program. Finally, as a scientist, I am deeply committed to fostering science education in the community. In addition to my teaching responsibilities at UCSF and VAMC, I have actively participated in the Science and Education Partnership program in San Francisco since 2000. My laboratory provides opportunity for a group talented high school students to pursue research experience in the summer. During their internship, the students are introduced to the basic principles of biomedical research. They are provided with ample opportunities to have hands-on experiences in my lab. Toward the end of the internship, students will have formal oral and poster presentations to showcase this unique educational experience. Perhaps the most rewarding part of this experience has been to see these talented students develop a high affinity for biomedical research and to eventually pursue a career in health professions. Indeed, essentially all of my students major in science-related disciplines in major universities, such as UC Berkeley, UCLA, UCSD, UC Davis, and University of Chicago.


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RESEARCH AND CREATIVE ACTIVITIES

RESEARCH AWARDS AND GRANTS

CURRENT

1) 1I01BX0011-8-01 (VA Merit Award) – Role: PI, Title: “Mechanisms of ER Stress and

Neurodegeneration in Parkinson’s Disease”, annual direct cost: $150,000, 7/1/2011-6/30/2015, 15% effort.

2) University of California MRPI Grant: University of California Pediatric Neuropathology Consortium (UCPNC), Award #142675 – Role: PI and Director of the Host Campus at UCSF. Annual direct cost: $352,000; 1/1/2010-12/31/2015.

3) Consortium for Frontotemporal Dementia Research (CFR), Role: PI. Annual direct cost: $110,000; 2008-2013.

4) Muscular Dystrophy Association (MDA), Research Grant #217592 – Role: PI, Title: Mechanism of FUS Mutations in Motor Neuron Survival and Synaptogenesis, proposed $125,000 per year, duration: 2/1/2012 – 1/31/2015

5) NIH OD011915-01 – Role: PI, Title: “Cellular and Animal Models of FUS mutation in ALS”, annual direct cost: $150,000, 15% effort.

6) P01NS083513 – Role: Core B Director & Project 3 Co-PI, Title: Regulation of Cellular Pathways in Human Brain Development, annual direct cost: $1,000,000, 10% effort

PENDING

1) NINDS/NIH R21 NS086668 – Role: PI, “Mechanism of neuroinflammation in the pathogenesis of TBI”

2) NINDS/NIH T32 NS086734 – Role: PI, “Experimental Neuropathology Training Program”

PAST

1) NIH K26 OD010927-04 – Role: PI, Title: “Mouse models of neuroprotection in dopamine

neurons (Mid-career Investigator Award), annual direct cost: $144,000, 2/1/2009 – 6/30/14, 50% effort.

2) Dept of Defense (DOD) – Role: PI, Title: “Mechanism of ER stress-mediated cell death in neurodegeneration and toxin-induced injury”, Annual direct cost: $150,000, 7/1/11-6/30/13.

3) NINDS/NIH 1RO1 NS048393-06 – Role: PI, Title: “Signaling Pathways of HIPK2 in Neuronal Apoptosis”, annual direct cost: $187,500, 12/04-12/10.

4) Alumni Fellowship: State University of New York (SUNY) Downstate Medical School, Role: Sponsor for medical student ’s summer research.

5) Development Travel Fellowship – Role: Mentor for Dr. Mianzhi Tang to travel to the Karolinska Institute in Sweden for projects related to embryonic stem cells, direct cost: $2,500

6) Dept of Defense (DOD) – Role: PI, Title: “Protect dopamine neurons from neurotoxin-induced

degeneration by targeting TGF signaling”, annual direct cost: $150,000, 8/1/07-7/31/09. 7) NINDS/NIH KO2 NS44223-04 (Independent Scientist Award) – Role: PI, Title: “Molecular

Mechanism of Sensory Neurogenesis”, 9/02-8/07, $168,750 per year, 70% effort

8) American Parkinson Disease Association – Role: PI, Title: “Role of TGF-HIPK2 signaling in protecting MPTP-induced toxicity to dopamine neurons”, 9/1/06-8/31/07, total annual cost: $50,000.


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9) Summer Student Fellowship – Role: Mentor, sponsored student: Ben Crittenden, Cambridge University, UK, 7/1/07-8/31/07, total cost: $1,700, & , Oberlin College, 7/1/08-8/31/08, total cost: $1,700.

10) The Michael J. Fox Foundation for Parkinson’s Research – Role: PI, Title: “Mechanism of HIPK2-dependent survival and differentiation of midbrain dopaminergic neurons – a chemical genetic target for Parkinson’s disease”. 01/01/05-12/31/05, total annual cost: $85,313.

11) Presidential Early Career Awards for Scientists and Engineers (PECASE), VA Medical Research - Role: PI, 9/1/2000 – 8/31/2005, total annual cost: $25,000.

12) The ALS Association – Role: PI, Title: Characterization of neurotrophic factor receptor expression in motor neurons. 9/1/2003 – 8/31/2004, total annual cost: $75,000.

13) The Advanced Research Career Award – Role: PI, Title: “Transcriptional control of neuronal survival and differentiation”. 10/1/99-9/30/02, total annual cost: $175,000.

14) The Whitehall Foundation – Role: PI, Title: “Transcriptional control of neuronal survival and axon pathfinding. 9/1/01-8/31/04, total annual cost: $75,000.

PEER REVIEWED PUBLICATIONS:

1. Huang, E., Nocka, K., Bier, D.R., Chu, T.Y., Buck, J., Lahm, H.M., Wellner, D., Leder, P. and Besmer, P. The Hematopietic Growth Factor KL Is encoded by the Sl Locus and Is the Ligand of

the c-kit Receptor, the Gene Product of the W Locus. Cell 63(1): 225-233, 1990. [Featured

Article]

2. Huang, E.J., Nocka, K.H., Buck, J. and Besmer, P. Differential Expression and Processing of Two Cell Associated Forms of the Kit-Ligand: KL-1 and KL-2. Mol. Biol. Cell 3(3): 349-362, 1991.

3. Cheng, J., Roques, B.P., Gacel, G.A., Huang, E. and Pasternak, G.W. k3 Opiate receptor binding in the mouse and rat. Eur. J. Pharmacol. 226: 15-20, 1991.

4. Manova, K., Bachvarova, R.F., Huang, E.J., Sanchez, S., Pronovost, S.M., Valezaquez, E., McGuire, B. and Besmer, P. C-KIT Receptor and Ligand Expression in Postnatal Development of the Mouse Cerebellum Suggests A Function for c-kit in Inhibitory Interneurons. J. Neurosci. 12(12): 4663-4676, 1992.

5. Pandiella, A., Bosenberg, M.W., Huang, E.J., Besmer, P. and Massague, J. Cleavage of Membrane-anchored Growth Factors Involved Multiple Protease Activities Regulated Through Common Mechanisms. J. Biol. Chem. 267: 24028-24033, 1992.

6. Huang, E.J., Manova, K., Packer, A.I., Sanchez, S., Bachvarova, R.F. and Besmer, P. The Murine Steel Panda Mutation Affects Kit-ligand Expression and Growth of Early Ovarian Follicles. Dev. Biol. 157(1): 100-109, 1992.

7. Manova, K., Huang, E.J., Angeles, M., DeLeon, V., Sanchez, S., Pronovost, S.M., Besmer, P and Bachvarova, R.F. The Expression Pattern of the c-kit Ligand in Gonads of Mice Supports a Role of the c-kit Receptor in Oocyte Growth and in Proliferation of Spermatogonia. Dev. Biol. 157(1): 85-99, 1992.

8. Huang, E.J., Wright, T.L., Lake, J.R., Comb, C. and Ferrell, L.D. Hepatitis B and C coinfections and persistent hepatitis B infections: Clinical outcome and liver pathology after transplantation. Hepatology 23(3): 396-404, 1995.

9. Tajima, Y., Huang, E.J., Vosseller, K., Ono, M., Moore, M.A.S. & Besmer, P. Role of Dimerization of the Membrane-associated Growth Factor Kit Ligand in Juxtacrine Signaling: The Sl

17H Mutation Affects Dimerization and Stability - Phenotype in Hematopoiesis. J. Exp. Med.

187: 1451-1461, 1996.


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10. Huang, E.J., Wilkinson, G.A., Fariñas, I., Backus, C., Zang, K., & Reichardt, L.F. Expression of Trk receptors in the developing mouse trigeminal ganglion: in vivo evidence for NT-3 activation of TrkA and TrkB in addition to TrkC. Development 126: 2191-2203, 1999.

11. Huang, E.J., Zang, K., Schmidt, A., Saulys, A., Xiang, M. & Reichardt, L.F. POU domain factor Brn-3a controls the differentiation and survival of trigeminal neurons by regulating the expression of Trk receptors. Development 126: 2869-2852, 1999.

12. Fan, G., Copray, S., Huang, E.J., Walro, J., Jaenisch, R. and Kucera, J. Formation of a full complement of cranial proprioceptors requires multiple neurotrophins. Dev. Dyn. 218:359-370, 2000.

13. Kim, W-Y, Fritzsch, B., Serls, A., Bakel, L.A., Huang, E.J., Reichardt, L.F., Barth, D.S. and Lee, J.E. NeuroD-null mice are deaf due to a severe loss of the inner ear sensory neurons during development. Development 128:417-426, 2001.

14. Huang, E.J., Liu, W., Fritzsch, B., Bianchi, L.M., Reichardt, L.F. and Xiang, M. Brn-3a is a transcriptional regulator of soma size, target field innervation and axon pathfinding of inner ear sensory neurons. Development 128, 2421-2432, 2001.

15. Fariñas, I., Jones, K.R., Tessarollo, L., Vigers, A.J., Huang, E.J., Kirstein, M., de Caprona, D.C., Coppola, D., Backus, C., Reichardt, L.F. and Fritzsch, B. Spatial shaping of cochlear innervation by temporally-regulated neurotrophin expression. J. Neurosci. 21:6170-6180, 2001.

16. Chan, T.T., Brown, E.J., Huang, E.J. and Seaman, W.M. Expression and activation of signal

regulatory protein on astrocytomas. Cancer Res. 64:117-127, 2004. 17. Wiggins, A.K., Wei, G., Doxakis, E., Wong, C., Tang, A.A., Zeng, K., Luo, E.J., Neve, R.L.,

Reichardt, L.F. and Huang, E.J. Interaction of Brn3a and HIPK2 mediates transcriptional repression of sensory neuron survival. J Cell Biol 167 (2): 257-267, 2004.

18. Doxakis, E., Huang, E.J. and Davies, A.M. Homeodomain interacting protein kinase 2 (HIPK2) regulates apoptosis in developing sensory and sympathetic neurons. Curr Biol 14(19): 1761-1765, 2004.

19. Huang, E.J., Li, H., Tang, A.A., Wiggins, A.K., Neve, R.L., Zhong, W., Jan, L.Y. and Jan, Y.N. Targeted deletion of Numb and Numblike reveals their essential functions in axon arborization.

Genes & Dev 19(1): 138-151, 2005 [Selected for Cover Art].

20. Ishikura, N., Clever, J.L., Bouzamondo Berstein, E., Prusiner, S.B., Huang, E.J. and DeArmond, E.J. Notch-1 activation and dendritic atrophy in prion disease. Proc Natl Acad Sci (USA) 102, 886-891, 2005.

21. Lynn, S., Huang, E.J., Elchuri, S., Naeemuddin, M., Nishinaka, Y., Yodoi, J., Ferriero, D.M., Epstein, C.J. and Huang, T.T. Selective neuronal vulnerability and inadequate stress response in superoxide dismutase mutant mice. Free Rad Biol Med 38, 817-828, 2005.

22. Zhang, J. and Huang, E.J. Dynamic Expression of Neurotrophic Factor Receptors in Postnatal Spinal Motoneurons and Mouse Model of ALS. J Neurobiol 66, 882-895, 2006.

23. Kauppinen, T., Alano, C.C., Tran, A.D., Wiggins, A.K., Huang, E.J. and Swanson, R.A. Direct phosphorylation and regulation of poly(ADP-ribose) polymerase-1 by extracellular signal-regulated kinases 1/2. Proc Natl Acad Sci (USA) 103(18): 7136-41, 2006.

24. Zhang, J., Pho, V., Bonasera, S.J., Holzmann, J., Helmuth, J., Tang, A.A., Janak, P.H., Tecott,

L.H. and Huang, E.J. Essential function of HIPK2 in TGF-dependent survival of midbrain

dopamine neurons. Nature Neuroscience 10:77-86, 2007. [Featured in News and Views]

25. Laposa, R., Huang, E.J. and Cleaver, J.E. Increased apoptosis, p53 upregulation, and cerebellar neuronal degeneration in repair deficient Cockyane Syndrome mice. Proc Natl Acad Sci (USA) 104: 1389-1394, 2007.


15

26. Wei, G., Ku, S., Saito, S., Tang, A.A., Mao, J.H., Appella, E., Balmain, A. and Huang, E.J.

HIPK2 represses -catenin-mediated transcription, epidermal stem cell expansion and skin tumorigenesis. Proc Natl Acad Sci (USA) 104: 13040-13045, 2007.

27. Spilman, P., Lessard, P., Sattavat, M., Bush, C., Tousseyn, T., Huang, E.J., Giles, K., Golde, T.,

Das, P., Fauq, A., Prusiner, S.B. and DeArmond, S.J. A -secretase inhibitor and quinacrine reduce prions and prevent dendritic degeneration in murine brains. Proc Natl Acad Sci (USA) 105: 10595-10600, 2008.

28. Tang, M., Miyamoto, Y. and Huang, E.J. Multiple roles of -catenin in controlling the neurogenic niche for midbrain dopamine neurons. Development 136: 2027-2038, 2009.

[Featured Article]

29. Loh, S.A., Carlson, G.A., Chang, E.I., Huang, E., Palanker, D., Gurtner, G.C. Comparative healing of surgical incisions created by the PEAK PlasmaBlade, conventional electrosurgery, and a scalpel. Plast Reconstruct Surgery 124: 1849-59, 2009.

30. Su, X., Kells, A.P., Huang, E.J., Lee, H.S., Hadaczek, P., Beyer, J., Bringas, J., Pivirotto, P., Penticuff, J., Eberling, J., Federoff, H.J., Forsayeth, J. and Bankiewicz, K.S. Safety Evaluation of AAV2-GDNF Gene Transfer Into the Dopaminergic Nigrostriatal Pathway in Aged and Parkinsonian Rhesus Monkeys. Human Gene Therapy 20: 1627-40, 2009.

31. Tartaglia, M.C., Sidhu, M., Laluz, V., Racine, C., Rabinovici, G.D., Creighton, K., Karydas, A.,

Rademakers, R., Huang, E.J., Miller, B.L., Dearmond, S.J. and Seeley, W.W. Sporadic coritcobasal syndrome due to FTLD-TDP. Acta Neuropath 119: 365-74, 2010.

32. Munesue, T., Yokoyama, S., Nakamura, K., Anitha, A., Yamada, K., Hayashi, K., Asaka, T., Liu, H.X., Jin, D., Koizumi, K., Islam, M.S., Huang, J.J., Ma, W.J., Kim, U.H., Kim, S.J., Park, K., Kim, D., Kikuchi, M., Ono, Y., Nakatani, H., Suda, S., Miyachi, T., Hirai, H., Salmina, A., Pichugina, Y.A., Soumarokov, A.A., Takei, N., Mori, N., Tsujii, M., Sugiyama, T., Yagi, K., Yamagishi, M., Sasaki, T., Yamasue, H., Kato, N., Hashimoto, R., Taniike, M., Hayashi, Y., Hamada, J., Suzuki, S., Ooi, A., Noda, M., Kamiyama, Y., Kido, M.A., Lopatina, O., Hashii, M.,

Amina, S., Malavasi, F., Huang, E.J., Zhang, J., Shimizu, N., Yoshikawa, T., Matsushima, A., Minabe, Y., Higashida, H. Two genetic variants of CD38 in subjects with autism spectrum

disorder and controls. Neurosci Res 67(2):181-91, 2010. [Featured Article]

33. Huang, E.J., Zhang, J., Geser, F., Trojanowski, J.Q., Strober, J.B., Dickson, D.W., Brown, R.H., Jr., Shapiro, B.E. and Lomen-Hoerth, C. Extensive FUS-immunoreactive Pathology in Juvenile amyotrophic lateral sclerosis with Basophilic Inclusions. Brain Pathology 20: 1069-76, 2010.

34. Tang, M., Villaescusa, J.C., Luo, S.X., Guitarte, C., Lei, S., Miyamoto, Y., Maketo, M.M.,

Arenas, E. and Huang, E.J. Interactions of Wnt/-catenin signaling and sonic hedgehog regulate the neurogenesis of midbrain dopamine neurons. J Neurosci 30: 9280-9, 2010.

35. Reyes, N.A., Fisher, J.K., Austgen, K., VandenBerg, S., Huang, E.J. and Oakes, S.A. Blocking the mitochondrial apoptotic pathway protects against neurodegeneration. J Clin Invest 120: 3673-9, 2010.

36. Min, S.W., Cho, S.H., Zhou, Y., Schroeder, S., Haroutunian, V., Seeley, W.W., Huang, E.J., Shen, Y., Masliah, E., Mukherjee, C., Meyers, D., Cole, P.A., Ott, M., and Gan L. Acetylation of tau inhibits its degradation and contributes to tauopathy. Neuron 67(6): 953-66, 2010.

37. Chang, E.I., Carlson G.A., Vose, J.G., Huang, E.J. and Yang, G.P. Comparative healing of rat fascia following incisions with three surgical instruments. J Surg Res 167(1): e47-54, 2011.


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38. Ruidiaz, M.E., Messmer, D., Atmodjo, D.Y., Vose, J.G., Huang, E.J., Kummel, A.C., Rosenberg, H.L., Gurtner, G.C. Comparative Healing of Human Cutaneous Surgical Incisions Created by the PEAK PlasmaBlade, Conventional Electrosurgery, and a Standard Scalpel. Plast Reconstr Surg. 128(1): 104-111, 2011.

39. Zhou, J., Tan, T., Tian, Y., Zheng, B., Ou, J.-H.J., Huang, E.J. and Yen, T.S.B. KLF15 activates hepatitis B virus gene expression and replication. Hepatology 54(1): 109-21, 2011.

[*Corresponding Author]

40. Lee, C., Hu, T., Nguyen, J.M., Zhang, J., Huang, E.J. and Chan, J.Y. Loss of Nrf1 function in the brain causes age-dependent neurodegeneration. Proc Natl Acad Sci (USA) 108(20): 8408-13, 2011.

41. Fancy, S.P.J., Harrington, E.P., Baranzini, S.E., Zhao, C., Silberesis, J.C., Otero, J.J., Huang,

E.J., Nusse, R., Franklin, R.J.M. and Rowitch, D.H. Wnt inhibitor AXIN2 as regenerative medicine target in multiple sclerosis and neonatal white matter injury. Nature Neurosci 14(8): 1009-16, 2011.

42. Lee, S.E., Rabinovici, G.D., Mayo, M.C., Wilson, S.M., Seeley, W.W., Dearmond, S.J., Huang,

E.J., Trojanowski, J.Q., Growdon, M.E., Jang, J.Y., Sidhu, M., See, T.M., Karydas, A.M., Gorno-Tempini, M.L., Boxer, A.L., Weiner, M.W., Geschwind, M.D., Rankin, K.P., Miller, B.L. Clinicopathological correlations in corticobasal degeneration. Ann Neurol 70(2): 327-40, 2011.

43. Chalazonitis, A., Tang, A.A., Shang, Y., Pham, T.D., Hsieh, I., Setlik, W., Gershon, M.D. and

Huang, E.J. Homeodomain Interacting Protein Kinase 2 Regulates Postnatal Development of Enteric Dopamine Neurons and Glia via BMP Signaling. J Neurosci 31(39): 13746-57, 2011.

[Cover Art Selection] 44. Sanai, N., Nguyen, T., Ihrie, R.A., Mirzadeh, Z., Tsai, H.H., Wong, M., Gupta, N., Berger, M.S.,

Huang, E., Garcia-Verdugo, J.M., Rowitch, D.H. and Alvarez-Buylla, A. Corridors of Migratory Neurons in Human Brain and their Decline During Infancy. Nature 478(7369): 382-6, 2011.

45. Na, B., Huang, Z., Wang, Q., Qi, Z., Tian, Y., Lu, C.C., Yu, J., Hanes, M.A., Kakar, S., Huang,

E.J., Ou, J.-H.J., Liu, L. and Yen, T.S.B. Transgenic Expression of Entire Hepatitis B Virus in Mice Induces Hepatocarcinogenesis Independent of Chronic Liver Injury. PLoS One 6(10): e26240, 2011.

46. Hammer, G.E., Turer, E.E., Taylor, K.E., Fang, C.J., Advincula, R., Oshima, S., Barrera, J.,

Huang, E.J., Hou, B., Malynn, B.A., Reizis, B., Defranco, A., Criswell, L.A., Nakamura, M.C., Ma, A. Expression of A20 by dendritic cells preserves immune homeostasis and prevents colitis and spondyloarthritis. Nature Immunol 12(12): 1184-93, 2011.

47. Rabinovici, G.D., Rosen, H.J., Alkalay, A., Kornak, J., Furst, A.J., Agarwal, N., Mormino, E.C.,

O'Neil, J.P., Janabi, M., Karydas, A., Growdon, M.E., Jang, J.Y., Huang, E.J., Dearmond, S.J., Trojanowski, J.Q., Grinberg, L.T., Gorno-Tempini, M.L., Seeley, W.W., Miller, B.L., Jagust, W.J. Amyloid vs FDG-PET in the differential diagnosis of AD and FTLD. Neurology 6;77(23):2034-42, 2011.

48. Mao, J.H., Wu, D., Kim, I.J., Kang, H.C., Wei, G., Climent, J., Kumar, A., Pelorosso, F.G.,

Delrosario, R., Huang, E.J. and Balmain, A. Hipk2 cooperates with p53 to suppress -ray radiation-induced mouse thymic lymphoma. Oncogene 31(9): 1176-40, 2012.

49. Kim, E.J., Sidhu, M., Gaus, S., Macedo, M.N., Huang, E.J., Miller, B.J., DeArmond, S.J., Seeley, W.W. Frontoinsular von Economo neuron and fork cell degeneration in behavioral variant frontotemporal dementia. Cerebral Cortex 22(2): 251-9, 2012.

50. Arnold, T.D., Ferrero, G.M., Qiu, H., Phan, I.T., Akhurst, R.J., Huang, E.J. and Reichardt, L.F. Defective Retinal Vascular Endothelial Cell Development As a Consequence of Impaired


17

Integrin αVβ8-Mediated Activation of Transforming Growth Factor-β. J Neurosci 32(4): 1197-206, 2012.

51. Revet, I., Feeney, L., Tang, A.A., Huang, E.J. and Cleaver, J.E. Dysmyelination not demyelination causes neurological symptoms in preweaned mice in a murine model of Cockayne syndrome. Proc Natl Acad Sci (USA) 109(12): 4627-32, 2012.

52. Kwan, K.Y., Lam, M.M.S., Johnson, M.B., Dube, U., Shim, S., Rasin, M-R., Sousa, A.M.M., Fertuzinhos, S., Chen, J.G., Arellano, J.I., Chan, D.W., Pletikos, M., Vasung, L., Rowitch, D.H.,

Huang, E.J., Schwartz, M.L., Willemsen, R., Oostra, B.A., Rakic, P., Heffer, M., Kostovic, I., Judas, M. and Sestan, N. Species-Dependent Posttranscriptional Regulation of NOS1 by FMRP in the Developing Cerebral Cortex. Cell 149, 899–911, 2012.

53. Sir, D., Kuo, C.F., Tian, Y., Liu, H.M., Huang, E.J., Jung, J.U., Machida, K., Ou, J.H. Replication of hepatitis C virus RNA on autophagosomal membranes. J Biol Chem 287(22): 18036-43, 2012.

54. Vossel, K.A., Bien-Ly, N., Bernardo, A., Rascovsky, K., Karydas, A., Rabinovici, G.D., Sidhu,

M., Huang, E.J., Miller, B.L., Huang, Y. and Seeley, W.W. ApoE and TDP-43 neuropathology in two siblings with familial FTLD-motor neuron disease. Neurocase 19(3): 295-301, 2013.

55. Nobuta, H., Ghiani, C.A., Paez, P.M., Spreuer, V., Dong, H., Korsak, R.A., Manukyan, A., Li, J.,

Vinters, H.V., Huang , E.J., Rowitch, D.H., Sofroniew, M.V., Campagnoni, A.T., de Vellis, J. and Waschek, J.A. STAT3-Mediated astrogliosis protects myelin development in neonatal brain injury. Ann Neurol. 72(5): 750-65, 2012

56. Martens, L.H., Zhang, J., Zhou, P., Kamiya, S., Sun, B., Min, S.-W., Zhang, Y., Diaz-Ramirez,

G., Gan, L., Huang, E.J.* and Farese, Jr., R.V.* Progranulin deficiency promotes microglial activation, proinflammtory cytokine production, and aggravated neuronal loss in toxin-induced neurodegeneration. J Clin Invest 122(11): 3955-9, 2012 (*co-corresponding authors)

57. Caso, F., Gesierich, B., Henry, M., Sidhu, M., LaMarre, A., Babiak, M., Miller, B.L., Rabinovichi, G.D., Huang, E.J., Magnani, G., Filippi, M., Comi, G., Seeley, W.W. and Gorno-Tempini, M.L. Nonfluent/agrammatic PPA with in-vivo cortical amyloidosis and Pick’s disease pathology. Behav Neurol 26(1-2): 95-106, 2013.

58. Grinberg, L.T., Wang, X., Wang, C., Sohn, P.D., Theofilas, P., Sidhu, M., Arevalo, J.B.,

Heinsen, H., Huang, E.J., Rosen, H., Miller, B.L., Gan, L., Seeley, W.W. Argyrophilic grain disease differs from other tauopathies by lacking tau acetylation. Acta Neuropath 125(4):581-93, 2013.

59. Filiano, A.J., Martens, L.H., Young, A.H., Warmus, B.A., Zhou, P., Diaz-Ramirez, G., Jiao, J.,

Zhang, Z., Huang, E.J., Gao, F.B., Farese, Jr. R.V. and Roberson, E.D. Dissociation of frontotemporal dementia-related deficits and neuroinflammation in Progranulin haploinsufficient mice. J Neurosci 33(12): 5352-5361, 2013.

60. Shang, Y., Doan, C., Arnold, T.D., Lee, S., Tang, A.A., Reichardt, L.F. and Huang, E.J.

Transcriptional Corepressors HIPK1 and HIPK2 Control Angiogenesis via TGF--TAK1-depdendent Mechanisms. PLoS Biology 11(4):e1001527, 2013.

61. Tang, M., Luo, S.X., Tang, V. and Huang, E.J. Temporal and spatial requirements of smoothened in ventral midbrain neuronal development. Neural Dev 8(1): 8, 2013.

62. Heng, M.Y., Lin, S.T., Verret, L., Huang, Y., Kamiya, S., Padiath, Q.S., Tong, Y., Palop, J.J., Huang, E.J., Ptacek, L.J. and Fu, Y.H. Lamin B1 mediates cell-autonomous neuropathology in a leukodystrophy mouse model. J Clin Invest 123(6): 2719-29, 2013.

63. Yang, D., Baumann, J.M., Sun, Y.Y., Tang, M., Dunn, R.S., Akeson, A.L., Kernie, S.G.,

Kallapur, S., Lindquist, D.M., Huang, E.J., Potter, S.S., Liang, H.C. and Kuan C,Y.


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Overexpression of vascular endothelial growth factor in the germinal matrix induces neurovascular proteases and intraventricular hemorrhage. Sci Trans Med 5(193):193ra90, 2013.

64. Siegenthaler, J.A., Choe, Y., Patterson, K.P., Hsieh, I., Li, D., Jaminet, S.C., Daneman, R.,

Kume, T., Huang, E.J. and Pleasure, S.J. Foxc1 is required by pericytes during fetal brain angiogenesis. Bio Open 2(7): 647-59, 2013.

65. Qiu, H.*, Lee, S.

*, Shang, Y., Wang, W.Y., Au, K.F., Kamiya, S., Barmada, S.J., Finkbeiner, S.,

Lui, H., Tang, A.A., Oldham, M.C., Wang, H., Shorter, J., Filiano, A.J., Roberson, E.D.,

Tourtellotte, W.G., Chen, B., Tsai, L.H. and Huang, E.J. ALS-associated mutation FUS-R521C causes profound dendritic and synaptic phenotype due to transcription and splicing defects. (in revision)

66. Wang WY, Pan L, Su SC, Quinn EJ, Sasaki M, Jimenez JC, Mackenzie IR, Huang EJ, Tsai LH. Interaction of FUS and HDAC1 regulates DNA damage response and repair in neurons. Nat Neurosci. 2013 Oct;16(10):1383-91.

67. Sánchez-Juan P, Ghosh PM, Hagen J, Gesierich B, Henry M, Grinberg LT, O'Neil JP, Janabi M,

Huang EJ, Trojanowski JQ, Vinters HV, Gorno-Tempini M, Seeley WW, Boxer AL, Rosen HJ, Kramer JH, Miller BL, Jagust WJ, Rabinovici GD. Practical utility of amyloid and FDG-PET in an academic dementia center. Neurology. 2014 Jan 21;82(3):230-8.

68. Caso F, Mandelli ML, Henry M, Gesierich B, Bettcher BM, Ogar J, Filippi M, Comi G, Magnani

G, Sidhu M, Trojanowski JQ, Huang EJ, Grinberg LT, Miller BL, Dronkers N, Seeley WW, Gorno-Tempini ML. In vivo signatures of nonfluent/agrammatic primary progressive aphasia caused by FTLD pathology. Neurology. 2014 Jan 21;82(3):239-47.

69. Qiu H*, Lee S

*, Shang Y, Wang WY, Au KF, Kamiya S, Barmada SJ, Finkbeiner S, Lui H, Tang

AA, Oldham MC, Wang H, Shorter J, Filiano AJ, Roberson ED, Tourtellotte WG, Chen B, Tsai

LH, Huang EJ. ALS-associated mutation FUS-R521C causes DNA damage and RNA splicing defects. J Clin Invest. 2014 Mar 3;124(3):981-99. [Featured in JCI Scientific Show Stopper: http://www.jci.org/posts/132 and Alzforum: http://www.alzforum.org/news/research-news/whats-fus-about-new-als-mouse-model-thickens-plot](*Equal contributions)

70. Fancy SP, Harrington EP, Baranzini SE, Silbereis JC, Shiow LR, Yuen TJ, Huang EJ, Lomvardas S, Rowitch DH. Parallel states of pathological Wnt signaling in neonatal brain injury and colon cancer. Nat Neurosci. 2014 Apr;17(4):506-12.

71. Hsieh CL, Niemi EC, Wang SH, Lee CC, Bingham D, Zhang J, Cozen M, Charo IF, Huang EJ, Liu J, Nakamura MC. CCR2 deficiency impairs macrophage infiltration and improves cognitive function after traumatic brain injury. J Neurotrauma. 2014 May 7. [Epub ahead of print]

REVIEWS AND CHAPTERS:

1. Besmer, P., Huang, E.J., Manova, K., Duttlinger, R. and Bachvarova, R.F. The kit-ligand (steel factor) and its receptor c-kit: pleiotropic roles in cell growth, cell survival and cell migration - insights from germline mutations. In "The pharmacology of cell differentiation." Rifkin, D.A. Ed. 1993, Elsevier Science Publisher.

2. Besmer, P., Manova, K., Duttlinger, R., Huang, E.J., Packer, A., Gyssler, C. and Bachvarova, R.F. The kit-ligand (steel factor) and its receptor c-kit/W: pleiotropic roles in gametogenesis and melanogenesis. Development. Supplement: 125-137.

3. Huang, E.J. and Reichardt, L.F. Neurotrophins: Roles in Neuronal Development and Function. Ann Rev Neurosci 24:677-736, 2001.

4. Huang, E.J. and Reichardt, L.F. Trk receptors: Roles in Neuronal Signal Transduction. Ann Rev Biochem 72:609-642, 2003.

http://www.jci.org/posts/132

http://www.alzforum.org/news/research-news/whats-fus-about-new-als-mouse-model-thickens-plot

http://www.alzforum.org/news/research-news/whats-fus-about-new-als-mouse-model-thickens-plot


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5. Silbereis, J.C., Huang, E.J., Back, S.A., Rowitch, D.H. Towards improved animal models of neonatal white matter injury associated with cerebral palsy. Dis Model Mech. 3:678-88, 2010.

6. Cleaver, J.E., Bezrookove, V., Revet, I. and Huang, E.J. Conceptual developments in the cause of Cockyane syndrome. Mech Ageing Dev 134 (5-6): 284-90, 2013.

THESIS:

1. Huang. E.J. Genetic Analysis and Biochemical Characterization of the Kit-Ligand - a Pleiotropic Growth Factor Encoded by the Murine Steel Locus. Ph.D. Thesis, Cornell University, Graduate School of Medical Sciences, N.Y., NY.

SUMMARY OF RESEARCH PROGRAMS Cell Biology of Midbrain Dopaminergic (DA) Neurons

DA neurons in the substantia nigra (SN) and ventral tegmental area (VTA) control a number of

important psychomotor behaviors, from motor learning to addictive behaviors. Dysfunction or degeneration in DA neurons has been implicated in several neuropsychiatric disorders, such as Parkinson’s disease and schizophrenia. Understanding the molecular mechanisms that govern the differentiation, survival, and target innervation in DA neurons will provide important insights to the

pathogenesis of these debilitating diseases. Previously, we have shown that Wnt--catenin and sonic hedgehog (Shh)-Smo signaling mechanisms regulate the expansion of DA progenitors and

differentiation of DA neurons during embryonic development. Our recent efforts focus on how TGF signaling mechanisms regulate survival, dendritic growth and synaptic plasticity of DA neurons. We

use mouse genetics and molecular and cell biology to characterize how TGF type II receptor (TRII) and transcriptional cofactor HIPK2 affect survival, homeostasis and maintenance of DA neurons in postnatal life. Furthermore, we also use experimental paradigms of neurodegeneration to determine whether the same signaling mechanisms can be “hijacked” to promote neuronal dysfunction and degeneration in human disease conditions.

Molecular and Cellular Mechanisms of FTLD and ALS

Frontotemporal lobar degeneration (FTLD) is the second most common neurodegenerative disease in patients younger than 60 years old. A significant number of FTLD patients develop motor neuron degeneration with cellular and molecular signatures similar to those seen in patients with amyotrophic lateral sclerosis (ALS). To understand mechanisms that connect these two devastating diseases, we focus on genes in which mutations have been causally linked to human diseases. Specifically, our results show that FTLD gene Progranulin (PGRN) is required to suppress neuroinflammation in injury or toxin-induced neurodegenerative conditions. Loss-of-function in PGRN promotes neurodegeneration through activation of microgliosis and increases in proinflammatory cytokines. Our current effort focuses on how PGRN deficiency causes both cell autonomous and non-cell autonomous defects leading to neurodegeneration in the aging process. Finally, we are interested in characterizing the similarities and differences among three different ALS mouse models, ALS-SOD1, ALS-FUS and ALS-TDP43. Our efforts to compare and contrast these different models should provide new insights to the pathogenesis and therapeutics for both familial and sporadic ALS.

Prenatal and Postnatal Human Brain Development


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Our ability to examine postmortem human brains from stages as early as the second trimester to advanced aging provides a unique opportunity to investigate many important questions about human brain development. It also allows us to determine how diseases might disrupt or interfere with critical processes, such as dendrite formation and circuit formation. We have developed both independent and collaborative efforts that target the cellular and molecular mechanisms of neurological diseases, including germinal matrix hemorrhage, perinatal hypoxic ischemic injury, and neurodegenerative diseases, such as Parkinson’s disease, ALS, FTLD and Alzheimer’s disease. To further expand our scope of research, we have developed a concerted effort to systematically collect and examine brains from a wide range of age spectrum. While the complexity of human brain is daunting, we believe the resources developed through our efforts hold the keys to unlock many critical questions about human diseases, including the pathogenesis of complex neuropsychiatric diseases, such as autism and schizophrenia.

List of most significant recent publications: Tang, M., Luo, S.X., Tang, V. and Huang, E.J. Temporal and spatial requirements of smoothened

in ventral midbrain neuronal development. Neural Dev 8(1): 8, 2013.

Shang, Y., Doan, C., Arnold, T.D., Lee, S., Tang, A.A., Reichardt, L.F. and Huang, E.J.

Transcriptional Corepressors HIPK1 and HIPK2 Control Angiogenesis via TGF--TAK1-depdendent Mechanisms. PLoS Biology 11(4):e1001527, 2013.

Chalazonitis, A., Tang, A.A., Shang, Y., Pham, T.D., Hsieh, I., Setlik, W., Gershon, M.D. and Huang, E.J. Homeodomain Interacting Protein Kinase 2 Regulates Postnatal Development of Enteric Dopamine Neurons and Glia via BMP Signaling. J Neurosci 31(39): 13746-57, 2011.

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university of california san francisco curriculum vitae · 2005 dean’s prize for research, ucsf...

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