university of milano-bicocca, milan final program · • two or more peaks with identical mass...

48TH International Symposium on High-PerformanceLiquid Phase Separations and Related Techniques

University of Milano-Bicocca, Milan

ALBERTO CAVAZZINIUniversità degli Studi di Ferrara

Chairs MASSIMO MORBIDELLIPolitecnico di Milano

F I N A L P R O G R A M

c

Maintaining your high standards requires analytical products you can rely on, along with the right documentation and expertise.

That's why our columns, cartridges, SPE tubes, SPME fi bres, plates, capillaries, solvents and accessories are precisely crafted to ensure you reliably achieve your analytical goals and comply with guidelines and regulations. So for work that hits the mark every time, choose Supelco®

chromatography products.

Supelco® chromatography products.Exceptional accuracy. Every time.

The Life Science Business of Merck operates as MilliporeSigma in the US and Canada.

Merck, the vibrant M and Supelco are trademarks of Merck KGaA, Darmstadt, Germany or its affi liates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.

© 2019 Merck KGaA, Darmstadt, Germany and/or its affi liates. All Rights Reserved.

For more information, please visit:SigmaAldrich.com/SuccessReplicated

CONTENTS

WELCOME FROM CHAIRMEN ..................................................................................................................... 3

MEDIA PARTNERS ................................................................................................................................................ 4

SPONSORS ................................................................................................................................................................. 5

COMMITTEES OF HPLC 2019 MILAN .................................................................................................... 7

HISTORY OF HPLC SYMPOSIUM AND FUTURE MEETINGS ..................................................8

SOCIAL EVENTS .................................................................................................................................................... 10

SPECIAL EVENTS ................................................................................................................................................. 13

AWARDS .................................................................................................................................................................... 15

GENERAL INFORMATION .............................................................................................................................25

EXHIBITORS AND CONGRESS VENUE FLOOR PLAN ................................................................32

PROGRAM AT A GLANCE ............................................................................................................................. 34

SHORT COURSES ................................................................................................................................................37

SCIENTIFIC SESSIONS .................................................................................................................................... 45

POSTER SESSIONS ............................................................................................................................................ 83

LIST OF SPONSORS & EXHIBITORS.....................................................................................................123

PRESENTING AUTHORS' INDEX ............................................................................................................133

1

2

www.facebook.com/Hplc2019-milan

www.hplc2019-milan.org

twitter.com/hplc2019

www.instagram.com/hplc2019milan

www.linkedin.com/company/hplc2019-milan/

Download the HPLC App for free from the main markets (Apple Store and Google Play)“Minimum system requirements required for the App: Android 6.0 and iOS 10”

ORGANIZING SECRETARIAT Effetti srl Via G.B. Giorgini, 16- 20151 Milan (I) [email protected] www.effetti.it

HPLC 2019

in

3

Dear Colleagues and Friends,We are very pleased to welcome you to HPLC 2019 from 16-20 June 2019 in Milan, Italy.

It is the first time that this conference - the most important international con-ference in the field of high-performance liquid phase separations and related techniques - is in Italy.

We have maintained the tradition of this conference by organizing an exciting meeting in a hospitable setting that is conducive to business, scientific and social exchange.

The conference covers both the fundamental and practical aspects of separa-tion science, with the main focus on new highly relevant trends that are emer-ging in this field. In particular we have encouraged younger scientists to make contributions in topical scientific sessions.

Situated in the northern part of Italy, Milan is a vibrant cosmopolitan center where the various cultures of northern European and Mediterranean coun-tries merge. Milan has a lot to offer: art museums, history, opera (La Scala), music, fashion, design, culinary delights and business opportunities.

This event will give you the possibility to focus on the newest scientific trends and professional developments in our field in an atmosphere of warm hospita-lity and the Italian expression of European union.

Welcome in Milan to this exciting event!

ALBERTO CAVAZZINIUniversità degli Studi di Ferrara

MASSIMO MORBIDELLIPolitecnico di Milan0

HPLC 2019 MILAN CHAIRMEN

WELCOME FROM THE CHAIRMEN

44

Exclusive Media Partner for filming and interviewing during HPLC2019 Milan

Media Premium Partner

Media Partners

Media Silver Partners

MEDIA PARTNERS

SPONSORS

We express our deepest gratitude to our Sponsors

Gold Sponsor

Platinum Sponsors

Silver Sponsors

Bronze Sponsors

�

5

S-Matrix software products are 100% aligned with Quality by Design (QbD) principles and regulatory guidances, scalable from standalone to global networks, and Citrix Ready certified. Perfect QbD software for Analytical R&D, Formulations, Chemical and Process R&D, and Respiratory Drug R&D and QC.

S-MATRIX IS A SOFTWARE SOLUTION PARTNER FOR

w w w . s m a t r i x . c o m

PeakTracker™ — Automated UV/MS Spectra Based Peak Tracking

Fast Chemistry ScreeningQuickly screen multiple column/solvent/pH/gradient combinations with automated pH prep, full solvent and column valve control, and data chromatogram visualization.

Robust Method OptimizationNew Rs-Map Response – hyper-accurate retention and peak parameter modeling, coupled with Monte Carlo Robustness simulation across the full design space. Graphics and simulation chromatograms show changes to your chromatography in real time as you change method conditions.

Complete QbD Design SpaceCharacterization, Visualization, and Reporting of the complete robust design space for all Critical Performance Metrics.

PeakTracker can automatically address these complex separation and tracking challenges: • Auto-deconvolution of partially and completely co-eluted peaks. • Two or more peaks with identical mass data. • Non-ionizable and non-absorbing compounds.

This powerful new UV/MS peak tracking technology automates, optimizes, and simplifies the use of PDA and MS data in LC and LC/MS method development. In addition to PDA spectral data, PeakTracker can utilize 3D mass spectral data for LC Systems configured with the Waters Acquity QDa Mass Detector.

Fusion QbD® — Automated LC and LC/MSMethod Development Software

Full Method Validation SuiteSystem SuitabilityFilter ValidationSample Solution StabilitySpecificityAccuracy

Linearity and RangePrecisionDetection LimitQuantitation LimitRobustness Verification

Calculations and Reporting Conform to All Current FDA, ICH, and USP Validation Guidances, including the new USP

NEW!

S-Matrix software products are 100% aligned with Quality by Design (QbD) principles and regulatory guidances, scalable from standalone to global networks, and Citrix Ready certified. Perfect QbD software for Analytical R&D, Formulations, Chemical and Process R&D, and Respiratory Drug R&D and QC.

S-MATRIX IS A SOFTWARE SOLUTION PARTNER FOR

w w w . s m a t r i x . c o m

PeakTracker™ — Automated UV/MS Spectra Based Peak Tracking

Fast Chemistry ScreeningQuickly screen multiple column/solvent/pH/gradient combinations with automated pH prep, full solvent and column valve control, and data chromatogram visualization.

Robust Method OptimizationNew Rs-Map Response – hyper-accurate retention and peak parameter modeling, coupled with Monte Carlo Robustness simulation across the full design space. Graphics and simulation chromatograms show changes to your chromatography in real time as you change method conditions.

Complete QbD Design SpaceCharacterization, Visualization, and Reporting of the complete robust design space for all Critical Performance Metrics.

PeakTracker can automatically address these complex separation and tracking challenges: • Auto-deconvolution of partially and completely co-eluted peaks. • Two or more peaks with identical mass data. • Non-ionizable and non-absorbing compounds.

This powerful new UV/MS peak tracking technology automates, optimizes, and simplifies the use of PDA and MS data in LC and LC/MS method development. In addition to PDA spectral data, PeakTracker can utilize 3D mass spectral data for LC Systems configured with the Waters Acquity QDa Mass Detector.

Fusion QbD® — Automated LC and LC/MSMethod Development Software

Full Method Validation SuiteSystem SuitabilityFilter ValidationSample Solution StabilitySpecificityAccuracy

Linearity and RangePrecisionDetection LimitQuantitation LimitRobustness Verification

Calculations and Reporting Conform to All Current FDA, ICH, and USP Validation Guidances, including the new USP

NEW!

7

SCIENTIFIC COMMITTEEAchille Cappiello Università di Urbino (Italy)Gert Desmet* Vrije Universiteit Brussel (Belgium)Paola Dugo Università di Messina (Italy)Attila Felinger* University of Pecs (Hungary)Michal Holčapek University of Pardubice (Czech Republic)Gérard Hopfgartner* University of Geneva (Switzerland)Robert T. Kennedy* University of Michigan (USA)Myeong Hee Moon Yonsei University (South Korea)Michael Lämmerhofer University of Tübingen (Germany)Gongke Li* Sun Yat-sen University (China)Susan Olesik* Ohio State University (USA)Koji Otsuka* Kyoto University (Japan)Janusz Pawliszyn University of Waterloo (Canada)Peter J. Schoenmakers* University of Amsterdam (The Netherlands)Jean Luc Veuthey University of Geneva (Switzerland)Caroline West University of Orléans (France)Mary Wirth Purdue University (USA)Guowang Xu* Dalian Institute of Chemical Physics, CAS (China)John Yates* The Scripps Research Institute (USA)Kelly Zhang* Genentech (USA)

*Permanent Scientific Committee Members

ORGANIZING COMMITTEEAlberto Cavazzini Università di FerraraMassimo Morbidelli Politecnico di MilanoLaura Cipolla Università Milano-BicoccaMartina Catani Università di FerraraMariosimone Zoccali Università di MessinaEduardo Sommella Università di SalernoOmar H. Ismail Università di FerraraAndrea Gargano University of AmsterdamGiorgia La Barbera "Sapienza" Università di RomaLuca Rivoira Università di TorinoMarco Roverso Università di PadovaSara Tengattini Università di PaviaJessica Fiori Università di BolognaThomas Themelis VUB BrusselsElena Barbaro Università di Venezia Cà FoscariFlavio Della Pelle Università di TeramoMonica Mattarozzi Università di Parma

COMMITTEES

8

HISTORY OF HPLC SYMPOSIUM

The International Symposium on High Performance Liquid Phase Separations (HPLC) is an international forum for the scientific discussion of methods of high-performance liquid chromatography in its various forms, along with the complementary separation techniques such as electrophoresis, electrochromatography, field-flow fractionation, supercritical fluid chromatography and hyphenated techniques, such as LC/MS, CE/MS and SFC/MS. In addition, mi-crofluidic, nanofluidic and chip separations, diagnostic systems, and other leading technologies are also considered.This symposium originated in 1973 in Interlaken, Switzerland. Advances in the field of liquid-phase separations are highlighted at each meeting. Meetings alternate between Europe and the United States, and odd years meetings in Europe are accompanied by another meeting in Asia/Australasia region.

Year Location Chair(s)1973 Interlaken, Switzerland Willy Simon1975 Wilmington, USA Jack J. Kirkland1977 Salzburg, Austria Joseph F.K. Huber1979 Boston, USA Barry L. Karger1981 Avignon, France Georges Guiochon1982 Cherry Hill, USA Robert Bardford1983 Baden-Baden, Germany Klaus Peter Hupe1984 New York City, USA Csaba Horvath1985 Edinburgh, UK John H. Knox1986 San Francisco, USA Ronald E. Majors1987 Amsterdam, The Netherlands Hans Poppe1988 Washington, USA Georges Guiochon1989 Stockholm, Sweden Douglas Westerlund1990 Boston, USA Barry L. Karger1991 Basel, Switzerland Fritz Erni1992 Baltimore, USA Fred E. Regnier1993 Hamburg, Germany Klaus K. Unger1994 Minneapolis, USA Larry D. Bowers and Peter W. Carr1995 Innsbruck, Austria Wolfgang Lindner1996 San Francisco, USA William S. Hancock1997 Birmingham, UK Anthony F. Fell1998 St. Louis, USA Daniel W. Armstrong1999 Granada, Spain Emilio Gelpi2000 Seattle, USA Edward S. Yeung2001 Maastricht, The Netherlands Hans Poppe and Henk Lingeman2001 Kyoto, Japan Nobuo Tanaka and Shigeru Terabe2002 Montreal, Canada Irving W. Wainer2003 Nice, France Antoine M. Siouffi2004 Philadelphia, USA Mark R. Schure2005 Stockholm, Sweden Douglas Westerlund2006 San Francisco, USA John H. Frenz2007 Gent, Belgium Jacques Crommen and Pat Sandra

1 Reoptimize Chiral Screening Protocols to Include Amylose tris(3-chloro-5-methylphenylcarbamate) as Chiral Selector of Exceptional Recognition Ability

2 Improving MS Sensitivity with Easy to Use Nano LC Columns

Attend our Vendor Seminar!Visit us in booth

#26

Monday, June 17 12:00 – 1:00 pm Hall 01F

PA67870419

ate)

9

2008 Baltimore, USA Georges Guiochon and Steven Jacobson2008 Kyoto, Japan Koji Otsuka and Nobuo Tanaka2009 Dresden, Germany Christian Huber2010 Boston, USA Steven A. Cohen2011 Budapest, Hungary Attila Felinger2011 Dalian, China Yukui Zhang and Peter Schoenmakers2012 Anaheim, USA Frantisek Svec2013 Amsterdam, The Netherlands Peter Schoenmakers and Wim Kok2013 Hobart, Australia Paul Haddad and Emily Hilder2014 New Orleans, USA J. Michael Ramsey2015 Geneva, Switzerland Gérard Hopfgartner2015 Beijing, China Guibin Jiang2016 San Francisco, USA Robert T. Kennedy2017 Prague, Czech Republic Michal Holcapek and Frantisek Foret2017 Jeju Island, Korea Doo Soo Chung2018 Washington, DC, USA Norman Dovichi2019 Milan, Italy Alberto Cavazzini and Massimo Morbidelli2019 Kyoto, Japan Koji Otsuka2020 San Diego, USA Mary Wirth2021 Düsseldorf, Germany Michael Lämmerhofer and Oliver J. Schmitz

HISTORY OF HPLC SYMPOSIUM

1 Reoptimize Chiral Screening Protocols to Include Amylose tris(3-chloro-5-methylphenylcarbamate) as Chiral Selector of Exceptional Recognition Ability

2 Improving MS Sensitivity with Easy to Use Nano LC Columns

Attend our Vendor Seminar!Visit us in booth

#26

Monday, June 17 12:00 – 1:00 pm Hall 01F

PA67870419

ate)

10

OPENING CEREMONY, CONCERT and WELCOME RECEPTION June 16, 2019 from 16.00 to 22.00

Milan Conservatorio “Giuseppe Verdi” Via Conservatorio, 12 20122 Milano www.consmilano.it

The Milan Conservatorio Giuseppe Verdi is the largest music academy in Italy. It was founded by Royal Napoleonic Decree in 1807 and opened on 3 September 1808 in the cloisters of a Baroque jewel, the Church of Santa Maria della Passione. In its 200-years history has educated some of the most prominent musicians and conductors and it conti-nues to be one of the most important national and international musical institutions, with more than 1,500 students, 236 teachers, 100 courses of study and a wide range of cultural events and orchestral concerts in the opulent con-cert hall “Sala Verdi”.

HOW TO REACH THE VENUEThe Milan Conservatorio is located right in the city centre and is easily accessible by public transport:

• Underground MM 1: stop S. Babila; take via Borgogna, after the traffic lights go straight on in Via Mascagni, then turn right into Via Conservatorio• Bus 54 or 61: east direction, stop Mascagni-Conservatorio, take Via Conservatorio to the right• Bus 54 or 61: west direction, stop Monforte-S. Damiano, go back and take Via Conservatorio to the right• Bus 94: stop V. Modrone-Mascagni, go through Via della Passione until the end. Milan Conservatorio will be in front of you

SOCIAL EVENTS

11

GALA DINNER June 19, 2019 from 20.30 to 24.00

Università degli Studi di Milano Via Festa del Perdono, 7 20122 Milano www.unimi.it

The Università degli Studi di Milano, founded in 1923, is one of the most important universities of Italy belonging to the League of European Research Universities (LERU). It is the largest University in Italy with 31 Departments and over 63.000 students. The headquarters are located in the Renaissance Palace of Ca’ Granda, build under Duke of Milan Francesco Sforza reign.The garden is surrounded by columns and pilasters of neoclassical design made by Filarete.Evening will consist of two different moments:• welcome standing cocktail in the garden• seated dinner along the cloister

To attend Gala Dinner you will find a voucher in the badge holderto be delivered on site

HOW TO REACH THE VENUEBy public transportationUnderground• M1 line or M3 line Duomo stop (600 mt walking distance)• M3 line Missori stop (300 mt walking distance)

Please take note that underground stops at 24.00

SOCIAL EVENTS

with the contribution of

Gala Dinner

Wednesday, 19th June 201920.30-24.00MILAN Central Courtyard of Università degli Studi di Milano

With the contribution of

SOCIAL EVENTS

The perfect fit for your analytical

HPLC& UHPLC Matching detectors according to your analytes

Most flexible pump configurations

Column kits ideal for your methods

Supporting OpenLAB, ClarityChrom® and Chromeleon™

VISIT US! BOOTH

6www.knauer.net

PROUD SPONSORS

SEPARATION SCIENCE

HPLC2019MILANOSlam

Science Together

FAREWELL DRINKJune 20, 2019 from 16.15 to 17.30

University of Milano-Bicocca Building U6 - Piazza dell'Ateneo Nuovo, 1 20126 Milano www.unimib.it

HPLC2019 Milan has reached its end and it is time to celebrate the time we have spent together: let’s have one last, great memory of our experience.Take a sip of Italian Spritz and share your final thoughts, ideas, and knowledge for the last time before your return to your friends, family, and loved ones.All participants and accompanying persons are kindly welcome.


13

Hall U6 08 SEPARATION SCIENCE SLAMJune 18, 2019 from 18.00 to 19.00Separation Science Slam is a competition for young scientist presentedfor the first time, at HPLC Symposium, in Milan.Projects will be presented (through a Power Point presentation) by the six finalists in a Separation Science Slam session planned on June 18th: on the Separation Science Slam stage the finalists will have to inform, engage and thrill the audience - and Jury - with the hard-ear-ned results of their liquid chromatography-based research project.

At the end of the session three winners will be announce by the Jury and a nice Apero will be offered to invited participants.

To participate in the Separation Science Slam Session you have to deliver the voucher.

Hall U6 08 HPLC TUBEJune 19, 2019 from 18.00 to 19.00 HPLCTube is a brand-new contest, proposed for the first time in the historyof HPLC Symposium. Unlike traditional scientific contests, application to HPLC Tube requires no paper, no abstract, but a video (max 3 minutes) on the presenting scientist’s own results. Submission is due by May 16, 2019.Among all videos, an international Jury will select the nominees for HPLC Tube final round which is scheduled at the University of Milano-Bicocca on June 19, from 18.00 to 16.00 pm.

At the end of the final round all participants will be invited to a nice Apero

To participate in the HPLC Tube Session you have to deliver the voucher.

With the contribution of SPECIAL EVENTS



SEPARATION SCIENCE Slam is a competition for young scientist presented

for the first time, at HPLC Symposium, in Milan.

Six finalist have been selected by the Slam Jury

and will present their projects during the

Separation Science Slam Session.THREE WINNERS announcement and a nice

APERO at the end of the Session

Tuesday, 18th June 201918.00-19.30Hall U6-08


SESSION

final roundHPLCTube is a brand-new contest,proposed for the �rst time in the historyof HPLC Symposium.Unlike traditional contests, applicationto HPLCTube required a video. Come to see the �nalists’ videos,

selected by the Jury


Wednesday 19th June 2019Hall U6-08 18.00-19.30

At the end of the �nal round all participants

will be invited to a nice Apero

15

AWARDS

GEORGES GUIOCHON FACULTY FELLOWSHIPAbout the Fellowship

The purpose of the Fellowship is to honor the memory of Georges Guiochon and recognize his major contribu-tions to HPLC, including his interest in fostering the careers of young people in separation science. The Fellow will be selected annually and will receive a $15,000 research grant and a commemorative plaque. The inaugural Fellow will be expected to present specially dedicated lectures at the HPLC 2019 symposium in Milano, Italy and at the HPLC 2020 symposium in San Diego, CA, USA for which travel support will be provided. The Fellowship is sponsored by HPLC, Inc. The award will be presented during the Opening Plenary Session on Sunday, June 16.

Eligibility CriteriaAll full-time faculty members at U.S. academic or government institutions who are within 10 years of their first independent research appointments at the time of the award are eligible for consideration. The selection process will be based on overall excellence in research in fields aligned with liquid phase separation science.

About Georges GuiochonProfessor Georges Guiochon (1931-2014) was born in France. He graduated in 1953 with an MS degree in engineering at Ecole Polytechnique (Paris, France) and received a Ph.D. in chemistry from the University of Paris (France) in 1958. He was a Professor of Chemistry at Ecole Polytechnique (1958-1985) and at the University Pierre et Marie Curie of Paris (1968-1984), then at Geor-getown University, Washington, D.C. (1984-1987). He was appointed a Distinguished Professor at the University of Tennessee (Department of Chemistry) and a Senior Scientist at the Oak Ridge National Laboratory (Division of Chemical Sciences) in 1987. Georges Guiochon was the undisputed master of the theory in almost all fields related to chromatography. He presented many rigorous treatments on retention and, especially, efficiency in liquid chromatography. He provided the theoretical foundation for the large-scale application of preparative chromatography, which is now one of the key technologies of the emerging biopharmaceutical industry. More recently, Georges Guiochon guided the re-emergence of supercritical-fluid chromatography in the fundamentally correct directions. No other scientist has demonstrated the breadth of knowledge, nor the unceasing moti-vation, that Georges Guiochon used to shape the field of chromatography to where it is today. His efforts garnered awards that included 2 from the ACS and the LCGC Lifetime Achievement Award. He received honorary doctoral degrees from the Universi-ties of Pardubice, Ramon Llull (Barcelona), Ferrara, and Science and Technology (Liaoning), and was inducted into the Spanish Academy of Science in 2011. He published 10 books and about 1100 peer-reviewed papers while performing research with over a hundred graduate students and post-doctoral fellows.

2019 Georges Guiochon Faculty Fellows AwardeeRyan Kelly, Brigham Young University, Provo, UTDr. Ryan Kelly is an Associate Professor in the Department of Chemistry and Biochemistry at Brigham Young University (BYU) and he holds a joint appointment at Pacific Northwest National Laboratory (PNNL). He re-ceived his Ph.D. in 2005 from BYU under the direction of Prof. Adam Woolley where he developed electrically driven microfluidic separations for biological analyses. He spent the next 13 years at Pacific Northwest National Laboratory (PNNL), first as a postdoctoral researcher with Dr. Richard D. Smith, and most recently serving as Technical Group Manager and Chief Technologist of PNNL’s Environmental Molecular Sciences Laboratory. Dr.

Kelly’s research efforts have focused on the development of new technological solutions for biochemical analyses. His team re-cently developed nanoPOTS, a microfluidic nanodroplet platform for preparing trace biological samples. In combination with ul-trasensitive LC/MS, nanoPOTS has enabled the first in-depth, label-free profiling of single mammalian cells. NanoPOTS has been applied to a wide range of trace biological systems through coupling with laser capture microdissection and fluorescence-acti-vate cell sorting, including circulating tumor cells, individual pancreatic islets and proteome imaging of mouse uterine models of embryo preimplantation. His team also recently developed novel nanowell-mediated two-dimensional nanoLC separations to profile ~6000 proteins from low-nanogram proteomic samples.

16

Past Faculty Fellows2015 Professor Amy E. Herr, University of California, Berkeley2016 Professor Ying Ge, University of Wisconsin-Madison2017 Professor Dwight Stoll, Gustavus Adolphus College2018 Professor Peter Nemes, University of Maryland, College Park2019 Professor Ryan Kelly, Brigham Young University, Provo, UT

UWE D. NEUE AWARD IN SEPARATION SCIENCEAbout the AwardThe Uwe D. Neue Award was created to recognize scientists that have made and continue to make significant contributions to the field of separation science, in honor of the legacy of Dr. Uwe D. Neue, late scientist and Waters® Corporate Fellow. The award will be given at the 48th International Symposium on High-Performan-ce-Liquid-Phase Separations and Related Techniques (HPLC 2019) held from 16-20 June 2019 in Milan, Italy. The award is sponsored by Waters Corporation.

Eligibility CriteriaThe award will honor a distinguished industrial scientist, 15-20 years or more after receiving his or her degree, who has made a significant contribution to the field of separation science. In addition, the awardee should be an industrial scientist, and one who was instrumental in the embodiment of technology in commercial products.

AwardeesThe award recipient will receive a commemorative plaque, a $5,000 check and travel support. He/she will have an opportunity to present a research lecture at the following HPLC Symposium. The 2019 award will be given at the 48th International Symposium on High-Performance-Liquid-Phase Separations and Related Techniques (HPLC 2019) held from 16-20 June 2019 in Milan, Italy. The recipient of the 2019 Uwe D. Neue Award in Separation Science is Dr. Christopher Welch. The award will be presented during the Opening Plenary Session on Sunday, June 16.

Past awardeesHPLC 2013 Dr. Jack KirklandHPLC 2014 Dr. Gerard RozingHPLC 2015 Dr. Mark SchureHPLC 2016 Dr. Lloyd SnyderHPLC 2017 Dr. Andrew AlpertHPLC 2018 Dr. Christopher PohlaHPLC 2019 Dr. Christopher Welch

CHROMATOGRAPHIC SOCIETY MARTIN MEDALThe Chromatographic Society is now pleased to announce that Professor Robert Kennedy from the University of Michigan will be awarded the Martin Medal for 2019. The award has been made in recognition of his “outstanding contribution to the development of innovative techniques in miniaturization of chemical separations and microfluidics for highly sensitive analysis of biological compounds”.

Professor Robert KennedyRobert Kennedy developed an interest in analytical chemistry and chemical separations while earning his BS degree in chemistry at the University of Florida. He became fascinated with the ability of GC to separate subtly different molecules while he was performing undergraduate research in organic chemistry. His analytical classes taught by Prof. John Dorsey further enhanced this interest. He went on to earn a PhD with James Jor-genson at University of North Carolina where is work focused on using open tubular LC to analyze single cells. After a post-doc with Mark Wightman, he started his own research program at University of Florida before mo-

ving to University of Michigan as the Hobart H. Willard Professor of Chemistry in 2002. His research has combined his lifelong in-

17

terest in biology with chemical analysis and separations. A theme of his group has been development of miniaturized, high-spe-ed separations for sensing, detection of non-covalent complexes, and screening. His group has developed capillary separation methods for monitoring neurotransmitters in vivo. These methods have been used for studying changes in neurotransmitter concentrations associated with behavior and diseases. His group has also developed microfluidic electrophoresis devices for monitoring insulin secretion from pancreatic β-cells. These methods are coupled with LC-MS metabolomics to understand the biochemical mechanism of insulin secretion and perturbations associated with diabetes. His group is also researching use of rapid electrophoretic and mass spectrometric assays for high-throughput screening. His work has been recognized by several awards including ACS Award in Chromatography, McKnight Award for Technical Innovations in Neuroscience, EAS Separation Science Award, Golay Award for Achievements in Chromatography, The Ralph Adams Award in Bioanalytical Chemistry and several teaching awards. He has held several service posts and is presently Associate Editor of Analytical Chemistry and Chair of the Chemistry Department at University of Michigan.Robert has been a leading separation science researcher for a number of years with many of his students and collaborators progressing into positions of seniority. It is with great pleasure The Chromatographic Society awards the 2019 Martin Medal to Professor Robert Kennedy this well-deserved honour will be presented to him at HPLC 2019 in Milan, during the Opening Plenary Session on Sunday, June 16.

This article is reproduced with the kind permission from the March 2019 edition of Chromatography Today.

JFK HUBER LECTURE AWARDThe JFK Huber Lecture Award was created in 2014 by the Austrian Society of Analytical Chemistry (ASAC) to ho-nor scientists who have made major contributions to the advancement of HPLC in theory and practice. Based on nominations the Honouring Committee of the ASAC will select the awardee. The Award will be presented at international conferences related to separation sciences. Awardees so far: 2014 Attila Felinger (Pécs) at ISC 2014 Innsbruck, Austria and 2017 Gert Desmet (Brussels) at HPLC 2017 Prague, CZ.Prof. Joseph Franz Karl Huber (1 Jan, 1925 – 15 Aug, 2000) who gives this Award the name, received his Ph.D. degree in 1960 about a physicochemical topic under the supervision of Prof. Erica Cremer from the University

Innsbruck (Austria). 1964 Joseph Huber moved to the Free University of Amsterdam (NL). In 1974 he moved back to Austria accepting the Chair of Analytical Chemistry at the University of Vienna where he became Emeritus in 1995. He is considered as one of the founding fathers of HPLC whereby his vision of the impact of small particles on the high efficiency of LC and of multi-dimensional LC marks the two corner stones of his research. The award will be presented during the Opening Plenary Session on Sunday, June 16.

2019 JFK Huber Lecture AwardeeFabrice G. Gritti, Waters Corporation, Milford, MAFabrice G. Gritti received a B.S. degree in Chemistry and Physics from the University Joseph Fourier of Greno-ble (France) in 1995, a Graduate Engineering School degree in Chemistry and Physics from the University of Bordeaux I (France) in 1997, and a Ph.D. in Chemistry and Physics of Condensed Matter from the University of Bordeaux I (France) in 2001. He came in the U.S. in 2002 for a post-doctoral visit at the University of Tennessee (Knoxville, TN) in the research group of Prof. Georges Guiochon. He worked there as a Research Scientist in the

Department of Chemistry until 2014. In 2015, he joined Waters Corporation as a principal research scientist in the Instrument/Core research/Fundamental department.Dr. Gritti’s research interests involve liquid/solid adsorption thermodynamics and mass transfer in heterogeneous media for characterization and design optimization of new liquid chromatography columns/instrument technologies. He developed experimental protocols that helped (1) refine the models of adsorption isotherms used in preparative chromatography for the prediction of the band profiles of neutral and ionizable compounds, (2) complete the theory of band broadening along modern analytical columns in linear chromatography, and (3) reshape columns and instruments to maximize performance and sensitivity in the field of separation science by liquid and supercritical fluid chromatography.Dr. Gritti has been invited to give 30 seminars, workshops, or tutorials on general chromatographic sciences at universities, discussion groups, or at international meetings. He has delivered over 50 invited keynote lectures at international Symposia and published over 270 peer-reviewed scientific papers. Dr. Gritti was the recipient of the 2013 Chromatographic Society Jubilee Medal for his important contribution to the development of chromatographic science.

AWARDS

18

CHROMATOGRAPHIC SOCIETY JUBILEE MEDALThe Chromatographic Society is pleased to announce the award of the Jubilee Medal to Professor Frederic Lynen from the Ghent University in recognition of his “pioneering work on novel column chemistry, sample preparation and high- end imple-mentation of state-of-the-art instrumentation to address separation problems in both quantitative and qualitative analysis”.

Professor Frederic LynenFrederic Lynen began his academic career at Ghent University in Belgium, where he obtained his PhD on the screening of combinatorial libraries via affinity electro- and pressure driven separation techniques in combina-tion with mass spectrometry under the supervision of Prof. Pat Sandra in 2002. He then moved to the Uni-versity of Stellenbosch in South Africa for postdoctoral research on the possibilities of hyphenated (electro) chromatographic separation techniques in natural product analysis. In 2004, he returned to Ghent University with the start-up of the Pfizer Analytical Research Centre (PARC). During this decade, long collaboration between academia and the pharmaceutical industry the transition towards greener, better resolving and faster

analytical separation processes in the pharmaceutical industry was prepared in various ways. He thereby further specialized in column technology through the manufacturing of temperature responsive stationary phases allowing purely aqueous HPLC while also supervising 15 PhD, postdoctoral and MSc research projects active in the fields of e.g. column technology, green chro-matography, matrix effects in mass spectrometry, oligonucleotide analysis, comprehensive HPLC, metabolite analysis, sample preparation and high efficiency SFC.Since 2011 Dr. Lynen is associate professor, in charge of the Separation Science Group in the Department of Organic and Macro-molecular Chemistry, at Ghent University. His research interests span a broad range of activities from fundamental (electro-) chromatographic performance predictions, improved predictive chromatographic modeling through Stationary Phase Optimize Liquid and Supercritical Fluid Chromatography (SOS-LC and SOS-SFC), stationary phase synthesis and column design for HPLC, SFC, GC and CEC, purely aqueous green temperature responsive liquid chromatography, the development various alternative 2-D LCxLC, LC-LC, LCxCE and GCxGC approaches, the design of enhanced separation and quantitation approaches specifically for medium sized and large molecules, matrix effects studies in mass spectrometry, immobilized artificial membrane chromato-graphy, universal detection, natural product and complex pharmaceutical analysis, and also biomarker discovery in the fra-mework of biomedical, art-historical and archeological research projects.Dr. Lynen has published about 120 papers (h-index of 28 in Scopus, citations number > 2300). He has given more than 40 presen-tations in national and international conferences. His (currently expanding) research group now comprises 6 PhD, 2 postdoc-toral and 2 MSc researchers, who have been awarded about 10 poster awards at major chromatographic conferences in recent years. In 2012, Frederic was awarded the Silver medal during the Method Development Olympics.Frederic is actively also involved in the support of the separation science community. This, for example, through his partici-pation to the editorial board of Journal of Chromatography A, as regular reviewer for the journals in the field of separation sciences and, last but not least, as chair of various editions (2012, 2014, 2016) of the International Conferences in Hyphenated Techniques in Chromatography (HTC) and Hyphenated Techniques for Sample Preparation (HTSP). He is also permanent chair of the Hyphenated Techniques in Chromatography section of the Flemish Royal Society of chemistry allowing for dissemination of the research in separation sciences to broader audiences. Frederic teaches a variety of course in including Organic Chemistry, Separation Methods, Organic Mass Spectrometry, Green Chemistry and Chemistry and Society courses in the Bachelor’s and Master’s programs at Ghent University, together with occasional specialized course in the line of the research activities.Professor Lynen has demonstrated his exceptional abilities, application and commitment to the field of separation science and as such, the Chromatographic Society is delighted to honour him with the Jubilee Medal for 2019. Frederic will receive his award at HPLC 2019 in Milan, during the Opening Plenary Session on Sunday, June 16.

This article is reproduced with the kind permission from the March 2019 edition of Chromatography Today.

19

BEST POSTER AWARDThe Best Poster Award (BPA) at HPLC 2019 Milan is sponsored by Agilent Technologies, a global leader in life sciences, diagno-stics, and applied chemical markets.Presentation of scientific work on a poster is an efficient way to disseminate and discuss results with peers, progress understan-ding of fundamental aspects of separation science, publish new instrumental methods and applications of chromatography and electrophoresis, especially for young scientists.Poster sessions undoubtedly belong to the most important parts of the HPLC 2019 Milan Symposium. Poster displays will be arranged to allow intensive and comprehensive review and discussion between authors and delegates.Posters will be reviewed by an international panel of scientists. The Chairman of the Best Poster Award Review Board is Dr. Gerard Rozing: a pioneer in HPLC technology. All participating posters will be evaluated by the following criteria:• Novelty, originality, and creativity of the work• The scope of the work, the quality of the experimental design and practical execution• Presentation of the work on the poster. Special emphasis will be on the author's presence and explanation during the desi-

gnated poster session.• Impact of the work like the potential for innovation, on progressing separation science or the research work involved, on

economic and societal aspects, or its use in science teachingThe Best Poster Award at HPLC 2019 Milano consists of a certificate and a money prize (400 Euro each for 12 awards) and will be delivered during the Closing Plenary Ceremony on Thursday, June 20.

CSABA HORVÁTH YOUNG SCIENTIST AWARDAbout the AwardThe purpose of the Award is to honor the memory of Csaba Horváth and recognize his contributions to HPLC, including his interest in fostering the careers of young people in separation science and engineering. The award includes an invitation to speak at the HPLC 2020 San Diego Symposium, a grant to support travel to that mee-ting, and a trophy engraved with the winner’s name. The award is sponsored by HPLC, Inc. The award will be presented during the Closing Ceremony on Thursday, June 20.

Eligibility CriteriaAll presenters of oral contributions (excepting past winners) who are less than 35 years of age at the time of their lecture are eligible for consideration. Candidates will be required to provide evidence of eligibility (e.g., passport, driver’s license).

Selection ProcessThe Scientific Committee selects abstracts for inclusion in the oral program. An Award Jury judges the eligible presentations and chooses a winner. The winner will be announced at the Closing Plenary Ceremony.

About Csaba HorváthProfessor Csaba Horváth (1930-2004) was born in Hungary and graduated in chemical engineering from the Budapest Institute of Technology. After receiving his Ph.D. in physical chemistry at the J.W. Goethe University in Frankfurt under the direction of Prof. Halász, he immigrated to the United States in 1963 and started research at the Harvard Medical School. In the following year, Dr. Horváth moved to Yale where he designed and built the first high performance liquid chromatograph to demonstrate the feasibility and potential of HPLC in bioseparation sciences. He chaired the Department of Chemical Engineering at Yale from 1987 to 1993 and was named as Roberto C. Goizueta Professor of Chemical Engineering in 1998. Professor Horváth contributed close to 300 publications to the field of separation sciences and had nine patents. His main topics were all fundamental aspects of separations, including instrumentation, stationary phase designs, and mechanisms of separation processes, as well as their application mainly to biological and biomedical research, especially for the high-resolution separation of proteins and peptides.

Past recipients of the Csaba Horváth Young Scientist AwardHPLC 2006 San Francisco, USA Norma Scully, University of Cork, IrelandHPLC 2007 Gent, Belgium Caterina Temporini, University of Pavia, ItalyHPLC 2008 Baltimore, USA Jude Abia, University of Tennessee, USAHPLC 2009 Dresden, Germany André de Villiers, Stellenbosch University, South AfricaHPLC 2010 Boston, USA Jesse Omamogho, University College Cork, Ireland

AWARDS

HPLC 2011 Budapest, Hungary Matthias Verstraeten, Free University of Brussels, BelgiumHPLC 2012 Anaheim, USA Stefan Bruns, Philipps-Universität Marburg, GermanyHPLC 2013 Amsterdam, The Netherlands James Grinias, University of North Carolina Chapel Hill, USAHPLC 2014 New Orleans, USA William Black, University of North Carolina Chapel Hill, USAHPLC 2015 Geneva, Switzerland Andrea Gargano, University of Amsterdam, The NetherlandsHPLC 2016 San Francisco, USA Simone Dimartino, University of Edinburgh, UKHPLC 2017 Prague, Czech Republic Bob Pirok, University of Amsterdam, The NetherlandsHPLC 2018 Washington, DC, USA Martina Catani, Università degli Studi di Ferrara, Italy

HPLC 2019 Milan Csaba Horváth Award Finalists1 . Akbal Laura, University of Geneva, Switzerland TUE 4.4 OC 1582. Baert Mathijs, Ghent University, Belgium WED 3.3 OC 2233 . Chapel Soraya, Institut des Sciences Analytiques, Lyon, France WED 3.1 OC 1764. Field Jennifer, University of Strathclyde, Glasgow, United Kingdom TUE 1.1 OC 755. Irlam Rachel, King's College London, United Kingdom MON 4.2 OC 446. Oliviero Giorgio, NIBRT, Dublin, Ireland TUE 1.2 OC 987. Pacheco-Fernández Idaira, Universidad de La Laguna, San Cristóbal de La Laguna, Spain TUE 1.3 OC 1238. Piendl Sebastian, Leipzig University, Germany WED 2.3 OC 2219. Sepsey Annamária, University of Pécs, Dep. of Analytical and Environmental Chemistry, Hungary TUE 3.3 OC 13010. Zestos Alexander, American University, Washington DC, USA MON 2.3 OC 6111. Zoccali Mariosimone, University of Messina, Italy TUE 4.4 OC 157

Chiral Europe 1910945->HPLC 2019 Sponsorship 210x150 Half-page Ad.2.indd 1 5/2/19 9:56 AM

21

AWARDS

TALANTA MEDALTalanta, it’s editors and editorial advisory board members, and Elsevier are pleased to announce that Profes-sor Janusz Pawliszyn, University Professor at the University of Waterloo, Ontario, Canada is the recipient of the Talanta Medal 2019.The medal, issued on a biennial basis, acknowledges outstanding achievements in analytical chemistry. The 2019 medal is awarded to Professor Pawliszyn in recognition of his numerous contributions to the field, especially his pioneering developments in solid phase extraction and related techniques, which are widely adopted by analysts for sample preparation. His current research focuses on the design of highly automated and integrated instru-

mentation for the isolation and separation of analytes from complex matrices.Professor Pawliszyn serves as Editor-in-Chief of TrAC – Trends in Analytical Chemistry and as Editor of Analytica Chimica Acta. In addition, he is on the editorial boards of several journals, and he has over 550 scientific publications, and has an impressive Hirsch Index of 84 and over 30,000 citations. He is a Fellow of the Royal Society of Canada and holds the Canada Research Chair and NSERC Industrial Research Chair in New Analytical Methods and Technologies.His work has been recognized by numerous past awards, including among others the 2017 Pittsburgh Conference Award, the 2015 Halász Medal Award for Separation Sciences, the 2015 Maria Sklodowska-Curie Medal, the 2011 Dal Stephen Dal Nogare Award in Chromatography, the 2010 ACS Award in Separation Science and Technology, and the 2010 Marcel Golay Award.The medal will be presented to Janusz Pawliszyn on Monday at the end of the Session MON 1.3.

2019 Talanta Medal AwardeeJanusz Pawliszyn, University of Waterloo, Waterloo, CanadaThe primary focus of Professor Pawliszyn's research program is the design of highly automated and integrated instrumentation for the isolation of analytes from complex matrices and the subsequent separation, identi-fication and determination of these species. He is an author of over 550 scientific publications and a book on Solid Phase Microextraction. His Hirsch Index (H-index) is 88. He is a Fellow of Royal Society of Canada, editor of Analytica Chimica Acta, Trends in Analytical Chemistry and a member of the Editorial Board of Journal of Se-paration Science and Journal of Pharmaceutical Analysis. He initiated a conference, "ExTech", focusing on new

advances in sample preparation and disseminates new scientific developments in the area, which meets every year in different part of the world. He received the 1995 McBryde Medal, the 1996 Tswett Medal, the 1996 Hyphenated Techniques in Chroma-tography Award, the 1996 Caledon Award, the Jubilee Medal 1998 from the Chromatographic Society, U.K., the 2000 Maxxam Award from Canadian Society for Chemistry, the 2000 Varian Lecture Award from Carleton University, the Alumni Achievement Award for 2000 from Southern Illinois University, the Humboldt Research Award for 2001, 2002 COLACRO Medal, 2003 Canada Research Chair, in 2006 he has been elected to the most cited chemists by ISI, in 2008 he received A.A. Benedetti-Pichler Award from Eastern Analytical Symposium, 2008 Andrzej Waksmundzki Medal from Polish Academy of Sciences, 2008 Manning Prin-cipal Award, 2010 Torbern Bergman Medal from the Swedish Chemical Society, 2010 Ontario Premier's Innovation Award, 2010 Marcel Golay Award, 2010 ACS Award in Separation Science and Technology, 2011 PittCon Dal Nogare Award, 2012 E.W.R. Steacie Award, 2013 Environmental Research and Development Award and 2013 LeSueur Memorial Award, 2015 Maria Skłodowska-Curie Medal from Polish Chemical Society, 2015 Halász Medal Award from the Hungarian Society for Separation Sciences, 2017 Pitt-sburgh Conference Analytical Chemistry Award, the 2017 Eastern Analytical Symposium Award for Outstanding Achievements in the Fields of Analytical Chemistry, 2018 ACS Award in Chromatography and 2018 North American Chemical Residue Workshop Excellence Award. He presently holds the University Professor, Canada Research Chair and Natural Sciences and Engineering Research Council of Canada Industrial Research Chair in New Analytical Methods and Technologies.

Chiral Europe 1910945->HPLC 2019 Sponsorship 210x150 Half-page Ad.2.indd 1 5/2/19 9:56 AM

Find out more at thermofi sher.com/chromatography

© 2019 Thermo Fisher Scientifi c Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientifi c and its subsidiaries unless otherwise specifi ed. AD72974-EN 0419S

Laboratories are constantly asked to do more with less. Built from the ground up,

Thermo Scienti� c™ HPLC and UHPLC instruments enable you to raise your productivity

to the next level and give you con� dence in your results. Time and cost associated

with staff training are minimized allowing your laboratory to meet ever-increasing

productivity demands, making it faster to bring products to market. With the largest

portfolio of LC solutions, we remain a steadfast and committed partner in your

endeavor to improve the world around us.

LC that takes your productivity to new heights

The collective power of chromatography

UNITY LAB SERVICES

HPLC 2019Visit thermo� sher.com/hplc2019 to request access to our HPLC 2019 talks and posters



Laboratories are constantly asked to do more with less. Built from the ground up,

Thermo Scienti� c™ HPLC and UHPLC instruments enable you to raise your productivity

to the next level and give you con� dence in your results. Time and cost associated

with staff training are minimized allowing your laboratory to meet ever-increasing

productivity demands, making it faster to bring products to market. With the largest

portfolio of LC solutions, we remain a steadfast and committed partner in your

endeavor to improve the world around us.

LC that takes your productivity to new heights


UNITY LAB SERVICES


UNITY LAB SERVICES

From workstation to global deployment, Thermo Scienti� c™ Chromeleon™ Chromatography Data

System (CDS) seamlessly scales to any business, from research through development to QC.

Delivering proven productivity gains and straightforward compliance, Chromeleon CDS provides a

unique uni� cation of work� ows for chromatography and mass spectrometry analysis. It’s cloud-ready

software that does it all, backed by a product team dedicated to keeping you one step ahead of the

next regulatory or market change. With the largest portfolio of chromatography solutions, we remain a

steadfast and committed partner in your endeavor to improve the world around us.



One CDS to do it all



24

C

M

Y

CM

MY

CY

CMY

K

AD_HPLC_2019_A4_PRINT.pdf 1 5/13/19 10:04 AM

25

CONGRESS VENUE University of Milano-Bicocca Building U6 Piazza dell’Ateneo Nuovo, 1 20126 Milano www.unimib.it

The University of Milano-Bicocca is a public, multidisciplinary University. With about 900 teachers and researchers and 33.000 enrolled students, Bicocca offers a wide range of degree courses in Economics-Statistics, Law, Medicine, Psychology, Science, Sociology and Education. Established in 1998 it was ranked - by the Times Higher Education ranking of the best 100 Universities under 50 years old - as number 21 worldwide and first in Italy. The Congress Centre is one of the largest and best equipped centers in Milan.

HOW TO REACH THE CONGRESS VENUEBy public transportationUnderground• M5 line Bicocca stop (950 mt walking distance) or Ponale stop (850 mt walking distance)• M1 line Precotto stop, then metrotramvia 7 toward Piazzale Cacciatori delle Alpi• M3 line Zara stop, then metrotramvia 7 toward AnassagoraFor more information see www.atm.it/en

GENERAL INFORMATION

26

OFFICIAL LANGUAGE English is the official Symposium language. No translation will be provided.

SHORT COURSES AND SYMPOSIUM DATES Short courses: Sunday June 16 from 09.30 to 13.00Symposium: from Sunday June 16 at 16.00 to Thursday June 20 at 17.30

REGISTRATION DESK OPENING HOURS Sunday June 16 08.30 - 13.00* 14.30 - 16.00**Monday June 17 08.00 - 18.30Tuesday June 18 08.00 - 19.30Wednesday June 19 08.00 - 19.30Thursday June 20 08.00 - 18.00*University of Milano-Bicocca**Milan Conservatorio “Giuseppe Verdi”

BADGE AND CONGRESS KIT Badge and congress kit will be delivered at HPLC 2019 registration desk:WHEN WHERE TIME WHATSunday June 16 University of Milano-Bicocca From 08.30 to 13.00 Badge and congress kit Milan Conservatorio "Giuseppe Verdi" From 14.30 to 16.00 Only badge*Monday June 17 University of Milano-Bicocca From 08.30 to 10.15 Badge and congress kitOther Congress days University of Milano-Bicocca During registration desk Badge and congress kit opening hours

*If you will attend the Opening Ceremony at Milan Conservatorio you will find in your personalized badge the pick up congress kit coupon.Delivery at Congress venue on Monday June 17.

All participants and exhibitors have to wear the name badge in the conference area completely visible at all times.

SCIENTIFIC SESSIONS Sunday June 16 09:30 - 13.00 Short courses 16.00 - 21.00 Opening Ceremony at Milan Conservatorio “Giuseppe Verdi” – Welcome receptionMonday June 17 10.15 - 18.35 Scientific sessionsTuesday June 18 08.30 - 19.30 Scientific sessionsWednesday June 19 08.30 - 19.30 Scientific sessions From 19.30 Gala Dinner at Università degli Studi di MilanoThursday June 20 08.30 - 12.55 Scientific sessions 14.00 - 17.30 Closing Plenary session – Farewell Drink

27

EXHIBITION AREA The exhibition is an important component of the conference, so please take the time to thank the exhibitors for their generous support of the program by visiting the booths located at Ground floor and First floor.

Exhibition Area opening hours:Monday June 17 2019 08.30 - 18.30Tuesday June 18 2019 08.30 - 19.30Wednesday June 19 2019 08.30 - 19.30Thursday June 20 2019 08.30 - 17.00

Exhibitor Pass and Media Pass only allow entry in the Exhibition Area and not allow entry in the scientific session.

LUNCHES Monday June 17 2019 12.20 - 14.00Tuesday June 18 2019 12.30 - 14.00Wednesday June 19 2019 12.40 - 14.00Thursday June 20 2019 12.55 - 14.00

INSTRUCTIONS FOR SPEAKERSAll presentations must be in Powerpoint 4:3 format or PDF and have to be handed over at the Slide Center Room at least 1 hour before the beginning of the session. It is not possible to bring presentations directly to the lecture hall. At the Slide Center Room, Speakers will have the possibility of:• Reviewing power-point presentations• Last minute changes of power-point presentations• Being supported by technical staff• Uploading power-point presentation in the dedicated session conference roomSlide Center Room is located at Ground Floor and will open from 08.00 every day of the Symposium.Speakers are recommended to arrive at least 20 minutes before the start of the session to introduce theirself to the session chairs.Speakers are strongly recommended to respect time limits for their talks as provided by their chairmen.

ORAL COMMUNICATION SESSIONS• Please arrive at your session at least 20 minutes before the start of the session to introduce yourself to the ses-

sion chair and to submit your presentation.• Kindly note that session chairs are under very strict instructions to keep their sessions on schedule. Suitable

devices to control the time and communicate it to the speakers are in each session hall. There are four sessions running in parallel with strict time constraints.

• Time allowed for presentation: 20 min to each Invited or award lecture and 15 min to each oral presentation selected by the Conference Scientific Committee. This timing includes discussion.

• Authors have to be synthetic and focus on the most important messages of their work.

GENERAL INFORMATION

28

POSTER SESSIONSAll posters have been divided into two poster viewing sessions: Session 1Set Up Monday 17 June 08:30-10:00Poster Viewing Monday 17 June 11:55-14:00 Monday 17 June 15:35-17:00 Tuesday 18 June 10:10-11:10Removal* Tuesday 18 June 12:30-13:00

Session 2Set Up Tuesday 18 June 13:00-14:00Poster Viewing Tuesday 18 June 15:45-16:35 Wednesday 19 June 10:20-11:20 Wednesday 19 June 12:40-14:00Removal* Wednesday 19 June 14:45-16:35

Best Poster Award Final Round Evaluation Thursday 20 June 10:05-11:10

*PLEASE NOTE:If you have been selected as Best Poster Award finalist (you will find a cockage on your poster), please do not remove your poster.If you don’t remove your poster as mentioned above, the Organizing Secretariat will remove it (Organizing Secretariat assumes no responsibility of problems or damage).

• Poster are located on the First Floor of the Venue (University of Milano-Bicocca)• Poster board numbers correspond to the poster presentation numbers in the final program• Authors are requested to stay by their poster during the entire duration of the corresponding Poster Session

HPLC 2019 BEST POSTER AWARDAmong abstracts accepted as Poster, 204 posters participate in the HPLC 2019 Best Poster Award. All BPA posters will be identified with a mark.25 posters will be selected for the Final Round evaluation, marked through a cockade and groupedtogether in a dedicated area (also on the first floor) on Thursday morning.The 12 winners will be announced on Thursday, June 20, 2019 during the Closing Plenary Session in Auditorium. Bes

t post

� Aw

�d F

inalis

t

29

SPECIAL ISSUE PUBLICATION - ELSEVIER - JCA/JCBAll authors of both oral and poster presentations are kindly invited to submit by 15th Sep 2019 manuscripts based your presentation(s) at the HPLC2019 Milan meeting for possible publication in Journal of Chromatography A or Jour-nal of Chromatography B, with the intention of publishing in a joint Special Issue that is dedicated to this symposium. For more information, please visit HPLC2019 Milan official website.

HPLC 2019 APPApp has been designed to promote the interaction between Participants, Faculty and Secretariat. You will find all the useful information to your participation, as well as in-depth files to support the discus-sed topics. By the App function “Messages” all Users can receive important communication from the Organizing Secretariat in real time.

You can find a dedicated HPLC 2019 Media Point at First Floor for any assistance and for asking access credentials.

HPLC 2019 WI FIIt is possible to connect to WIFI asking for access credentials at HPLC 2019 Media Point at First Floor.

GENERAL INFORMATION

VICI DBS WHISPER SERIES NITROGEN GAS GENERATORSFOR LC/MS INSTRUMENTS• Replaces costly nitrogen cylinders and gas dewars• LC/MS grade nitrogen, no phthalates or hydrocarbons• Ideal for curtain, shield, nebulizer and interface gases• Variable oxygen content increases LC/MS sensitivity• Saves valuable laboratory space through unique design

CHEMINERT M SERIES LIQUID HANDLING PUMPRATED UP TO 1500 PSI• Flow rates ranging from 5nL/min–5mL/min• Material options for biocampatibility (ceramic, PTFE, PEEK)• Pulseless, continuous, bi-directional flow• Syringe-free• Less than 0.5% error at low flow rates

www.vicidbs.com

www.vici.com

Read and submit your research to these HIGH-QUALITY JOURNALS FROM THE AMERICAN CHEMICAL SOCIETY

The world’s most trusted journal in protein analysis & function, offering the fastest time-to-publication.

The field’s most-cited journal, publishing original, innovative research across all branches of analytical chemistry.

ACS Omega is a fully open-access, global, multidisciplinary journal publishing high-quality research spanning the scope of the more than 55 ACS Publications journals and beyond.

pubs.acs.org

IMPACT FACTOR: 3.950IMPACT FACTOR: 6.042NOW WITH A $750 APC

30

31

GENERAL INFORMATION

ON SITE REGISTRATION On site registration can be made only by credit card.

Participant type On site Academic 950 €Industry 1.300 €Student* - Conference 400 €Student* - Conference + 2 Short Courses** 450 €Short course (each)** 200 €1 Day Registration 500 €Accompanying Person 140 € * Student must provide an official proof of the full-time student status** While seats last

Registration Fees (Academic, Industry and Student Conference) Include• Access to oral and poster sessions• Access to the exhibition hall• Welcome reception on Sunday - Milan Conservatorio "Giuseppe Verdi"• Coffee breaks and lunches on Monday, Tuesday, Wednesday and Thursday• Farewell drink on Thursday• Congress kit (delegate bag, badge, printed final program, electronic abstract book)• Milan public transportation tickets

Student Conference + 2 Short Courses Fee Includes• The same items as the registration fees above• 2 short courses

1 Day Registration Fee Includes• Access to oral and poster sessions• Access to the exhibition hall• Coffee breaks and lunch for the day• Congress kit (delegate bag, badge, printed final program, electronic abstract book)• Milan public transportation ticket

Accompanying Person Fee Includes• Welcome reception on Sunday• Coffee breaks and lunches on Monday, Tuesday, Wednesday and Thursday• Farewell drink on Thursday• Milan public transportation tickets

Cancellations and refundsNo refund will be made for cancellations.

32

8Hall U6-27Hall U6-26

Hall U6-28Hall U6-22Hall U6-23

Hall U6-29

U6-20Hall

U6-21Hall

U6-25Hall

U6-24Hall

lunch/coffee break

lunch/coffee break

lunch/coffee break

lunch/coffee break

Media Point

49

47

Poster &

lunch/

ENTRANCE FROM VIA PIERO E ALBERTO PIRELLI

ENTRANCE FROM PIAZZA ATENEO NUOVO

Hall U6-08

Hall U6-09U6-01F

U6-01A

HallU6-01E

HallU6-01D

Hall

HallU6-01B

HallU6-01CHall

Hall U6-07

Hall U6-06

Aula Magnacoffeebreak

coffeebreak

lunch/

lunch/

deskRegistration

Congress Kit pick up point

centerSlide

deskRegistration lunch/coffee break

1

2

3

45

6

79

MP2MP110

24

2522

27

22

13

14

45 4644

434241403938

373635

34333231

30

203212623

17

19 1815

12 1116

1

2

54 6

7 8

2829

SYMPOSIUM HALLS

BOOTHS

ORGANIZATIONAL SECRETARIAT

CATERING

X

YZ

POSTERS

TABLES

GROUND FLOOR PLAN building U6

FIRST FLOOR PLAN building U6

EXHIBITORS

PONALE 750 m

BICOCCA 950 m

VWR, part of Avantor

ACTIVATED RESEARCH COMPANY

GENOVIS

BIOTAGE AB, FROM SWEDENYMC EUROPE GmbH

KROMASIL BY NOURYON

AGILENT TECHNOLOGIES

TOSOH BIOSCIENCE

PHENOMENEX

ANALYTICAL SALES & SERVICES

ADVANCED CHEMISTRY DEVELOPMENT(ACD/LABS)

GL SCIENCES B.V.

LABOMATIC INSTRUMENTS AG

REGIS TECHNOLOGIES

HPLC 2021 DÜSSELDORF

ROTACHROM TECHNOLOGIES


CARLO ERBA REAGENTS S.R.L.

ALFATECH S.P.A.

MESTRELAB RESEARCH S.L.

FORTIS TECHNOLOGIES LTD

HTA S.R.L.

PEAK SCIENTIFIC

NACALAI TESQUE, INC.

MOLNÁR INSTITUTE for App ChrMACHEREY-NAGEL GmbH & Co. KG

SEPACHROM-MEGA S.R.L.

OPTIMIZE TECHNOLOGIES

PHARMAFLUIDICS

SILCOTEK GmbH

METROHM S.R.L.

1

2

3

4

5

WATERS CORPORATION

WATERS CORPORATION

S-MATRIX CORPORATION

VICI AG INTERNATIONAL

FUJI SILYSIA CHEMICAL SA

KNAUER

SHIMADZU

MERCK

SPECTRA ANALYSIS

MERCK

S.C.A.T. EUROPE GmbH

CPS ANALITICA S.R.L.

CHIRAL TECHNOLOGIES EUROPE

THERMO FISHER SCIENTIFIC

RESTEK CORPORATION

8

9

6

7

10

11

12

13

14

20

21

15

16

17

18

19 27

28

22

23

24

25

26

34

35

29

30

31

32

33 41

42

36

37

38

39

40

43

44

45

46

47

49

LC/GC

TRAJAN SCIENTIFICAND MEDICAL

BOOTHS

TABLES

HTC 2020 GHENT

THE ANALYTICAL SCIENTIST

SHARE FOR MEDIA

ISCC 2020 BUDAPEST

HPLC 2020 SAN DIEGO

HPLC 2019 KYOTO

MEETING POINTS

SHIMADZU

AGILENT TECHNOLOGIESMP1

MP2

1

2

3

4

5

6

33

8Hall U6-27Hall U6-26

Hall U6-28Hall U6-22Hall U6-23

Hall U6-29

U6-20Hall

U6-21Hall

U6-25Hall

U6-24Hall

lunch/coffee break

lunch/coffee break

lunch/coffee break

lunch/coffee break

Media Point

49

47

Poster &

lunch/

ENTRANCE FROM VIA PIERO E ALBERTO PIRELLI

ENTRANCE FROM PIAZZA ATENEO NUOVO

Hall U6-08

Hall U6-09U6-01F

U6-01A

HallU6-01E

HallU6-01D

Hall

HallU6-01B

HallU6-01CHall

Hall U6-07

Hall U6-06

Aula Magnacoffeebreak

coffeebreak

lunch/

lunch/

deskRegistration

Congress Kit pick up point

centerSlide

deskRegistration lunch/coffee break

1

2

3

45

6

79

MP2MP110

24

2522

27

22

13

14

45 4644

434241403938

373635

34333231

30

203212623

17

19 1815

12 1116

1

2

54 6

7 8

2829

SYMPOSIUM HALLS

BOOTHS

ORGANIZATIONAL SECRETARIAT

CATERING

X

YZ

POSTERS

TABLES

GROUND FLOOR PLAN building U6

FIRST FLOOR PLAN building U6

EXHIBITORS

PONALE 750 m

BICOCCA 950 m

VWR, part of Avantor

ACTIVATED RESEARCH COMPANY

GENOVIS

BIOTAGE AB, FROM SWEDENYMC EUROPE GmbH

KROMASIL BY NOURYON

AGILENT TECHNOLOGIES

TOSOH BIOSCIENCE

PHENOMENEX

ANALYTICAL SALES & SERVICES

ADVANCED CHEMISTRY DEVELOPMENT(ACD/LABS)

GL SCIENCES B.V.

LABOMATIC INSTRUMENTS AG

REGIS TECHNOLOGIES

HPLC 2021 DÜSSELDORF



CARLO ERBA REAGENTS S.R.L.

ALFATECH S.P.A.

MESTRELAB RESEARCH S.L.

FORTIS TECHNOLOGIES LTD

HTA S.R.L.

PEAK SCIENTIFIC

NACALAI TESQUE, INC.

MOLNÁR INSTITUTE for App ChrMACHEREY-NAGEL GmbH & Co. KG

SEPACHROM-MEGA S.R.L.

OPTIMIZE TECHNOLOGIES

PHARMAFLUIDICS

SILCOTEK GmbH

METROHM S.R.L.

1

2

3

4

5

WATERS CORPORATION

WATERS CORPORATION

S-MATRIX CORPORATION

VICI AG INTERNATIONAL

FUJI SILYSIA CHEMICAL SA

KNAUER

SHIMADZU

MERCK

SPECTRA ANALYSIS

MERCK

S.C.A.T. EUROPE GmbH

CPS ANALITICA S.R.L.

CHIRAL TECHNOLOGIES EUROPE

THERMO FISHER SCIENTIFIC

RESTEK CORPORATION

8

9

6

7

10

11

12

13

14

20

21

15

16

17

18

19 27

28

22

23

24

25

26

34

35

29

30

31

32

33 41

42

36

37

38

39

40

43

44

45

46

47

49

LC/GC

TRAJAN SCIENTIFICAND MEDICAL

BOOTHS

TABLES

HTC 2020 GHENT

THE ANALYTICAL SCIENTIST

SHARE FOR MEDIA

ISCC 2020 BUDAPEST

HPLC 2020 SAN DIEGO

HPLC 2019 KYOTO

MEETING POINTS

SHIMADZU

AGILENT TECHNOLOGIESMP1

MP2

1

2

3

4

5

6

EXHIBITORS AND CONGRESS VENUE FLOOR PLAN

34

SUNDAY 16/6 - University of Milano-Bicocca 9:30-13:00

Short Courses 1-8 9:30-11:00

Coffee break 11:00-11:30


SUNDAY 16/6 - Milan Conservatorio "Giuseppe Verdi" 16:00-22:00

Opening Ceremony

Awards 16:00-19:00

Plenary-1

Welcome Reception 19:00-22:00

MONDAY 17/6 - University of Milano-Bicocca 8:00-18:35

Registration/Poster set up 8:00-10:15

Hall U6-06 Hall U6-07 Hall U6-08 Hall U6-09

MON 1.1 MON 2.1 MON 3.1 MON 4.1 10:15-11:55 Proteomics-1 Miniat. & Elect. Tech. Fundamentals-1 Food Analysis-1

Lunch/Exhibition/Poster Session 11:55-14:00

Vendor seminars (12:20-13:20) (Agilent Technologies, Merck, Phenomenex, Waters Corporation)

MON 1.2 MON 2.2 MON 3.2 MON 4.2 14:00-15:35 Sample Prep.-1 Ion Exch. Chrom. & MS Fundamentals-2 Miniat. & Microfl.

Coffee break/Exhibition/Poster Session 15:35-17:00

MON 1.3 MON 2.3 MON 3.3 MON 4.3 17:00-18:35 Sample Prep.-2 Biomed. Appl. Fundamentals-3 Innovat. Appl.-1

TUESDAY 18/6 - University of Milano-Bicocca 8:30-19:00


TUE 1.1 TUE 2.1 TUE 3.1 TUE 4.1 08:30-10:10 LC-MS Bioact. Mol.-1 Multidim. Chrom.-1 Chiral Sep.-1 Environ. Analysis


TUE 1.2 TUE 2.2 TUE 3.2 TUE 4.2 11:10-12:30 LC-MS Bioact. Mol.-2 Multidim. Chrom.-2 Chiral Sep.-2 Pharm. & Biopharm.-1

Lunch/Exhibition/Poster removal-set up 12:30-14:00

Vendor seminars (13:00-14:00) (Agilent Technologies, Thermo Fisher Scientific, Shimadzu Europe GmbH, Molnár-Institute for Applied Chromatography)

TUE 1.3 TUE 2.3 TUE 3.3 TUE 4.3 14:00-15:45 Biosample Prep. Omics-1 Fundamentals-4 Capill. Electr.-1


TUE 1.4 TUE 2.4 TUE 3.4 TUE 4.4 16:35-18:00 Proteomics-2 Glycom. & Prot. Interact. Data Analysis-1 SFC-MS

SEPARATION SCIENCE SLAM 18:00-19:00

WEDNESDAY 19/6 - University of Milano-Bicocca 8:30-19:00


WED 1.1 WED 2.1 WED 3.1 WED 4.1 08:30-10:20 Forens. Doping & Tox. Pharm. & Biopharm.-2 HILIC Instrum. & Quantit.


WED 1.2 WED 2.2 WED 3.2 WED 4.2 11:20-12:40 Lipidomics 3D Printing & Microchip SFC-1 Food Analysis-2


Vendor seminars (13:00-14:00) (Agilent Technologies, Merck, Thermo Fisher Scientific, Waters Corporation)

WED 1.3 WED 2.3 WED 3.3 WED 4.3 14:00-15:45 Omics-2 Pharm. & Biopharm.-3 Fundamentals-5 Multidim. Chrom.-3

Coffee break/Exhibition/Poster removal 15:45-16:35

WED 1.4 WED 2.4 WED 3.4 WED 4.4 16:35-18:00 Omics-3 Miniat. & LC-MS Interf. QC-1 Pharm. & Biopharm.-4

HPLC TUBE 18:00-19:00

Gala Dinner 20:30-24:00 Università degli Studi di Milano

THURSDAY 20/6 - University of Milano-Bicocca 8:30-17:30


THU 1.1 THU 2.1 THU 3.1 THU 4.1 8:30-10:05 Capill. Electr.-2 Prep. Chrom.-1 HPTLC-1 SFC-2 & Data Analysis-2

Coffee break/Exhibition/Poster Session - Final Round 10:05-11:10

THU 1.2 THU 2.2 THU 3.2 THU 4.2 11:10-12:55 QC-2 Prep. Chrom.-2 HPTLC-2 Innovat. Appl.-2

Lunch/Exhibition 12:55-14:00

Plenary- 2 Closing Plenary Session 14:00-16:15

Farewell Drink 16:15-17:30

Plenary Short course Oral communication Vendor seminar Special event

pg. 38

pg. 41

pg. 46

pg. 46

pg. 46

pg. 84

pg. 90

pg. 97

pg. 104

pg. 47 pg. 47 pg. 48 pg. 48

pg. 66 pg. 67 pg. 67 pg. 68

pg. 68 pg. 69 pg. 69 pg. 70

pg. 109

pg. 115

pg. 72 pg. 72 pg. 73 pg. 73

pg. 74 pg. 74 pg. 75 pg. 75

pg. 76 pg. 77 pg. 77 pg. 78

pg. 78 pg. 79 pg. 79 pg. 80

pg. 81

pg. 76

pg. 70

pg. 51 pg. 52 pg. 52 pg. 53

pg. 53 pg. 54 pg. 54 pg. 55

pg. 55 pg. 56 pg. 56 pg. 57

pg. 58 pg. 58 pg. 59 pg. 59

pg. 61 pg. 62 pg. 62 pg. 63

pg. 64 pg. 64 pg. 65 pg. 65

pg. 66

pg. 60

pg. 49

35

SUNDAY 16/6 - University of Milano-Bicocca 9:30-13:00


Coffee break 11:00-11:30


SUNDAY 16/6 - Milan Conservatorio "Giuseppe Verdi" 16:00-22:00

Opening Ceremony

Awards 16:00-19:00

Plenary-1

Welcome Reception 19:00-22:00

MONDAY 17/6 - University of Milano-Bicocca 8:00-18:35

Registration/Poster set up 8:00-10:15


MON 1.1 MON 2.1 MON 3.1 MON 4.1 10:15-11:55 Proteomics-1 Miniat. & Elect. Tech. Fundamentals-1 Food Analysis-1


Vendor seminars (12:20-13:20) (Agilent Technologies, Merck, Phenomenex, Waters Corporation)

MON 1.2 MON 2.2 MON 3.2 MON 4.2 14:00-15:35 Sample Prep.-1 Ion Exch. Chrom. & MS Fundamentals-2 Miniat. & Microfl.


MON 1.3 MON 2.3 MON 3.3 MON 4.3 17:00-18:35 Sample Prep.-2 Biomed. Appl. Fundamentals-3 Innovat. Appl.-1

TUESDAY 18/6 - University of Milano-Bicocca 8:30-19:00


TUE 1.1 TUE 2.1 TUE 3.1 TUE 4.1 08:30-10:10 LC-MS Bioact. Mol.-1 Multidim. Chrom.-1 Chiral Sep.-1 Environ. Analysis


TUE 1.2 TUE 2.2 TUE 3.2 TUE 4.2 11:10-12:30 LC-MS Bioact. Mol.-2 Multidim. Chrom.-2 Chiral Sep.-2 Pharm. & Biopharm.-1

Lunch/Exhibition/Poster removal-set up 12:30-14:00

Vendor seminars (13:00-14:00) (Agilent Technologies, Thermo Fisher Scientific, Shimadzu Europe GmbH, Molnár-Institute for Applied Chromatography)

TUE 1.3 TUE 2.3 TUE 3.3 TUE 4.3 14:00-15:45 Biosample Prep. Omics-1 Fundamentals-4 Capill. Electr.-1


TUE 1.4 TUE 2.4 TUE 3.4 TUE 4.4 16:35-18:00 Proteomics-2 Glycom. & Prot. Interact. Data Analysis-1 SFC-MS

SEPARATION SCIENCE SLAM 18:00-19:00

WEDNESDAY 19/6 - University of Milano-Bicocca 8:30-19:00


WED 1.1 WED 2.1 WED 3.1 WED 4.1 08:30-10:20 Forens. Doping & Tox. Pharm. & Biopharm.-2 HILIC Instrum. & Quantit.


WED 1.2 WED 2.2 WED 3.2 WED 4.2 11:20-12:40 Lipidomics 3D Printing & Microchip SFC-1 Food Analysis-2


Vendor seminars (13:00-14:00) (Agilent Technologies, Merck, Thermo Fisher Scientific, Waters Corporation)

WED 1.3 WED 2.3 WED 3.3 WED 4.3 14:00-15:45 Omics-2 Pharm. & Biopharm.-3 Fundamentals-5 Multidim. Chrom.-3

Coffee break/Exhibition/Poster removal 15:45-16:35

WED 1.4 WED 2.4 WED 3.4 WED 4.4 16:35-18:00 Omics-3 Miniat. & LC-MS Interf. QC-1 Pharm. & Biopharm.-4

HPLC TUBE 18:00-19:00

Gala Dinner 20:30-24:00 Università degli Studi di Milano

THURSDAY 20/6 - University of Milano-Bicocca 8:30-17:30


THU 1.1 THU 2.1 THU 3.1 THU 4.1 8:30-10:05 Capill. Electr.-2 Prep. Chrom.-1 HPTLC-1 SFC-2 & Data Analysis-2

Coffee break/Exhibition/Poster Session - Final Round 10:05-11:10

THU 1.2 THU 2.2 THU 3.2 THU 4.2 11:10-12:55 QC-2 Prep. Chrom.-2 HPTLC-2 Innovat. Appl.-2

Lunch/Exhibition 12:55-14:00

Plenary- 2 Closing Plenary Session 14:00-16:15

Farewell Drink 16:15-17:30

Plenary Short course Oral communication Vendor seminar Special event

pg. 38

pg. 41

pg. 46

pg. 46

pg. 46

pg. 84

pg. 90

pg. 97

pg. 104

pg. 47 pg. 47 pg. 48 pg. 48

pg. 66 pg. 67 pg. 67 pg. 68

pg. 68 pg. 69 pg. 69 pg. 70

pg. 109

pg. 115

pg. 72 pg. 72 pg. 73 pg. 73

pg. 74 pg. 74 pg. 75 pg. 75

pg. 76 pg. 77 pg. 77 pg. 78

pg. 78 pg. 79 pg. 79 pg. 80

pg. 81

pg. 76

pg. 70

pg. 51 pg. 52 pg. 52 pg. 53

pg. 53 pg. 54 pg. 54 pg. 55

pg. 55 pg. 56 pg. 56 pg. 57

pg. 58 pg. 58 pg. 59 pg. 59

pg. 61 pg. 62 pg. 62 pg. 63

pg. 64 pg. 64 pg. 65 pg. 65

pg. 66

pg. 60

pg. 49

PROGRAM AT A GLANCE

SHORT COURSES

38

SHORT COURSE 1 09.30 - 11.00 INTRODUCTION OF GLP REGULATIONS AND BIOANALYTICAL METHOD VALIDATION BY LC-MS HALL U6 - 20 Speaker: P. Wang (LC-MS Technical Expert, USA)

Good laboratory practice (GLP) is a set of regulations intended to assure the quality and integrity of non-clinical laboratory studies that are intended to support research or marketing permits for products regulated by government agencies. The term GLP is most commonly associated with the pharmaceutical industry and the required non-clinical animal testing that must be performed prior to approval of new drug products.

"Guidance for Industry - Bioanalytical Method Validation" published in May 2018 provides general recommendations for bioanalytical method validation using advanced technologies. This short course will focus on GLP regulations and the bio-analytical method validation for drugs and metabolites in biological matrices using LC-MS. It will help audiences to comply with the regulations for drug discovery and development in the pharmaceutical industry and CROs. The course will also reflect the recently published white papers with regard to bioanalytical method validation using LC-MS.

This course will benefit the analytical chemists, lab supervisors, QA/QC managers, GLP auditors and CRO consultants who work in the GLP-regulated labs and the pharmaceutical industry. This course will also benefit all levels of management as a refresher course to stay current with the GLP regulations.

SHORT COURSE 2 09.30 - 11.00 GREEN SAMPLE PREPARATION FOR NEW GENERATION OF ANALYTICAL CHEMISTS: NEW CONCEPTS AND FUNDAMENTALS HALL U6 - 23 Speaker: J. Pawliszyn (University of Waterloo, Canada)

In this course several microextraction technologies will be discussed, namely solid phase microextraction (SPME), thin film microextraction (TFME) and needle trap devices (NT). The fundamental principles of these techniques, different calibra-tion methods, coupling strategies to GC, LC, and MS and advantages and disadvantages of each microextraction approach will be covered. In addition, during this short course, recent advances, developments and applications of each technique in fields such as forensic, environmental, food and flavor, and clinical analysis will be described including high-throughput formats compatible with LC and direct coupling to MS for screening applications. Participants interested in clinical and medical applications should consider to take course "BioSPME in clinical, pharmaceutical and biotechnological research" offered in the afternoon.

The course is targeted at HPLC 2019 participants, who wish to gain a deeper insight into the green sample preparation technologies:

PhD students Analytical chemists Scientists interested in on-site screening Laboratory supervisors Scientists and industry regulators

SHORT COURSE 3 09.30 - 11.00 ENANTIOSELECTIVE "CHIRAL" CHROMATOGRAPHY: FROM METHODOLOGICALCONCEPTS TO REAL WORLD APPLICATIONS HALL U6 - 24 Speakers: W. Lindner (University of Vienna, Austria), M. Lämmerhofer (University of Tübingen, Germany)

The aim of this Short Course is to discuss first the background and the general concepts of separating enantiomers via liquid chromatography with focus on the direct enantiomer separation technology. The basic principle relies on the forma-tion of diastereomeric intermediate associates between a chiral selector-moiety (the SO) and the enantiomeric analytes, the selectands (R)- and (S)-SAs. Conceptually the chemically very diversified chiral selector units get either strongly adsor-bed or chemically immobilized onto the surface of a support material, most of the time silica-based particles with a control-led porosity. Such a material represents the chiral stationary phase (CSP) which gets then packed into columns leading to the so-called "chiral" columns.

39

Sunday, June 16 2019

The formerly written diastereomeric [SO-(R)-SA] and [SO-(S)-SA] associates are physico-chemically different from each other in contrast to the non-associated (R)-SA and (S)-SA enantiomers. The binding strength of these two associates can be described by ΔG(R) and ΔG(S) values which are affected not only by the molecular structure and the steric motifs of the SO and of the SA moieties but also of the type of solvents and additives representing the mobile phase (MP) of the chro-matographic system. In reality one can use normal phase (NP) polar organic (PO) or reversed phase (RP) conditions. In addition also SFC type MP compositions can be well applied. With other words, these chromatographic systems represent a "Troika" which needs to be considered when discussing the overall retention and enantioselectivity criteria. Essential are the multiple intramolecular interactions defined by the interaction sites of the SO and the SAs moieties together with their spatial arrangement. They represent the molecular recognition principles. The differences of the ΔG( R) and ΔG(S) values described by the ΔΔG values of the diastereomeric associates are directly related to the observed stereoselectivity values alpha (a) of the enantioselective (the "chiral") chromatographic system.

In consideration of these basic principles we will then discuss the pros and cons of the most popular and promising so-cal-led "chiral" columns operated in various MP modes. To date there is a wide selection of CSPs and "chiral" columns ranging from quite broadly applicable but also to very dedicated ones. It encompasses columns packed with ultra-high efficient CSPs enabling ultra-fast enantiomer separation but also CSPs and column formats useful for preparative enantiomer sepa-ration. Via the presentation of real world examples and applications we aim to give an overview of the state-of-the-art of this technology and to stress also specific challenges depending on the analytical questions.

SHORT COURSE 4 09.30 - 11.00 SUPERCRITICAL FLUID CHROMATOGRAPHY (SFC) FUNDAMENTALS HALL U6 - 25 Speaker: C. West (University of Orleans, France)

This introductory course covers the basic elements of supercritical fluid chromatography (SFC). The basic theory of SFC and currently available commercial instrumentation is presented. The effects of operating parameters (choice of stationary phases and mobile phase composition, temperature, pressure) and specific aspects of detection (UV, ELSD, MS) are explai-ned in the perspective of method development. In addition, varied applications are discussed (pharmaceuticals, cosmetics, food science, environment ...).

Who Should Attend Everyone interested in using Supercritical Fluid Chromatography as an alternative for their current analysis, or those that

are already using SFC, but want a better understanding of the underlying principles of separation performance, retention and selectivity.

SHORT COURSE 5 09.30 - 11.00 NEW TRENDS IN LC-MS HALL U6 - 21 Speaker: A. Cappiello (University of Urbino, Italy)

This course is recommended to a wide audience of intermediate-level scientists, students and technicians in the field of analytical chemistry and related disciplines, such as biology, environment, clinical and food, among others. It will be also an extremely useful course for newcomers in the field willing to approach this subject for the first time. No previous knowle-dge of mass spectrometry is necessary, but an understanding of the fundamentals of liquid chromatography is beneficial. The focus has been addressed to the new trends, starting from the innovations of each technique (what is the vanguard in liquid chromatography; what is the new frontier in mass spectrometry) to the most progressive challenges of LC-MS. Researchers, practitioners, consultants working in any field in which LC-MS is the technique of choice will find this course valuable, with complete, up-to-date information on this fascinating and powerful technique. They will learn the state-of-the-art for the currently available LC-MS interfaces, their operational principles, as well as the 'new frontiers' and their application potential. A special focus has been given to 'high-end' topics such as high-resolution tandem mass spectrome-try, single ion monitoring, multiple reaction monitoring, as well as to data processing to achieve the highest sensitivity and selectivity.

SHORT COURSES

40

SHORT COURSE 6 09.30 - 11.00 3D-PRINTING IN SEPARATION SCIENCE HALL U6 - 26 Speaker: S. Dimartino (The University of Edinburgh, UK)

This short course aims to offer an overview of how 3D printing is impacting the separation sciences in general, and chroma-tography in particular. 3D printing can seamlessly create bespoke models with complex shapes, using a range of materials, and with r

university of milano-bicocca, milan final program · • two or more peaks with identical mass...

Documents