US Sequencing Project

• Funded by NSF• Two-year project• Start date: Sept 1, 2004• Follow-up project for full sequencing of

chromosomes 1, 10 and 11

Aims• 400,000 BAC end sequences• 2400 reads from sheared library• 20 full BAC sequences• FISH analysis for selected BACs• Bioinformatics hub for project

– Analysis pipelines– Data Archiving for entire project– Summary data for entire project– Links to other groups

Sequencing• Outsourced sequencing• BAC ends and sheared library:

– SeqWright Inc, Houston, Texas– ABI Prism 3730xl sequencers

• 20 full BACs– Phred/Phrap assembly

Bioinformatics• Clustering of BAC Ends, sheared sequences using SGN

pipeline• Gene coding potential analysis, repeat identification• BAC annotation using SGN pipeline based on GeneSeqer,

Apollo, gene finding programs

Sequence pipelines

• Clustering of BAC Ends, sheared sequences using SGN pipeline

• Gene coding potential analysis, repeat identification

• BAC annotation using SGN pipeline based on GeneSeqer, Apollo, gene finding programs