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Dissecting Lyme borreliosis; Clinical aspects, pathogenesis and prevention

Coumou, J.

Publication date2016Document VersionFinal published version

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Citation for published version (APA):Coumou, J. (2016). Dissecting Lyme borreliosis; Clinical aspects, pathogenesis andprevention. Boxpress.

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Download date:17 May 2021

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40. Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD (2006) Isolating and engineering human antibodies using yeast surface display. Nature protocols; 1: 755-68

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burgdorferi NapA-driven Th17 cell inflammation in lyme arthritis. Arthritis Rheum; 58: 3609-17 45. Constantin B (2014) Dystrophin complex functions as a scaffold for signalling proteins.

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British referral clinic. QJM : monthly journal of the Association of Physicians; 105: 537-43 47. Coumou J, Herkes EA, Brouwer MC, van de Beek D, Tas SW, Casteelen G, et al. (2015) Ticking

the right boxes: classification of patients suspected of Lyme borreliosis at an academic referral center in the Netherlands. Clin Microbiol Infect; 21: 368.e11-20

48. Coumou J, Hovius JW, van Dam AP (2014) Borrelia burgdorferi sensu lato serology in the Netherlands: guidelines versus daily practice. Eur J Clin Microbiol Infect Dis; 33: 1803-8

49. Coumou J, van der Poll T, Speelman P, Hovius JW (2011) Tired of Lyme borreliosis. Lyme borreliosis in the Netherlands. Neth J Med; 69: 101-11

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52. Dai J, Narasimhan S, Zhang L, Liu L, Wang P, Fikrig E (2010) Tick histamine release factor is critical for Ixodes scapularis engorgement and transmission of the lyme disease agent. PLoS Pathog; 6: e1001205

53. Dai J, Wang P, Adusumilli S, Booth CJ, Narasimhan S, Anguita J, et al. (2009) Antibodies against a tick protein, Salp15, protect mice from the Lyme disease agent. Cell Host Microbe; 6: 482-92

54. Daix V, Schroeder H, Praet N, Georgin JP, Chiappino I, Gillet L, et al. (2007) Ixodes ticks belonging to the Ixodes ricinus complex encode a family of anticomplement proteins. Insect molecular biology; 16: 155-66

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57. Dattwyler RJ, Luft BJ, Kunkel MJ, Finkel MF, Wormser GP, Rush TJ, et al. (1997) Ceftriaxone compared with doxycycline for the treatment of acute disseminated Lyme disease. N Engl J Med; 337: 289-94

58. de la Fuente J, Almazan C, Canales M, Perez de la Lastra JM, Kocan KM, Willadsen P (2007) A ten-year review of commercial vaccine performance for control of tick infestations on cattle. Animal health research reviews / Conference of Research Workers in Animal Diseases; 8: 23-8

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22. Branda JA, Aguero-Rosenfeld ME, Ferraro MJ, Johnson BJ, Wormser GP, Steere AC (2010) 2-tiered antibody testing for early and late Lyme disease using only an immunoglobulin G blot with the addition of a VlsE band as the second-tier test. Clin Infect Dis; 50: 20-6

23. Branda JA, Strle F, Strle K, Sikand N, Ferraro MJ, Steere AC (2013) Performance of United States serologic assays in the diagnosis of Lyme borreliosis acquired in Europe. Clin Infect Dis; 57: 333-40

24. Brehmer-Andersson E, Hovmark A, Asbrink E (1998) Acrodermatitis chronica atrophicans: histopathologic findings and clinical correlations in 111 cases. Acta Derm Venereol; 78: 207-13

25. Breitner-Ruddock S, Wurzner R, Schulze J, Brade V (1997) Heterogeneity in the complement-dependent bacteriolysis within the species of Borrelia burgdorferi. Med Microbiol Immunol; 185: 253-60

26. Brettschneider S, Bruckbauer H, Klugbauer N, Hofmann H (1998) Diagnostic value of PCR for detection of Borrelia burgdorferi in skin biopsy and urine samples from patients with skin borreliosis. J Clin Microbiol; 36: 2658-65

27. Brissette CA, Bykowski T, Cooley AE, Bowman A, Stevenson B (2009) Borrelia burgdorferi RevA antigen binds host fibronectin. Infection and immunity; 77: 2802-12

28. Brissette CA, Cooley AE, Burns LH, Riley SP, Verma A, Woodman ME, et al. (2008) Lyme borreliosis spirochete Erp proteins, their known host ligands, and potential roles in mammalian infection. Int J Med Microbiol; 298 Suppl 1: 257-67

29. Brooks CS, Vuppala SR, Jett AM, Alitalo A, Meri S, Akins DR (2005) Complement regulator-acquiring surface protein 1 imparts resistance to human serum in Borrelia burgdorferi. J Immunol; 175: 3299-308

30. Brown EL, Kim JH, Reisenbichler ES, Hook M (2005) Multicomponent Lyme vaccine: three is not a crowd. Vaccine; 23: 3687-96

31. Bunikis J, Barbour AG (2002) Laboratory testing for suspected Lyme disease. Med Clin North Am; 86: 311-40

32. Burgdorfer W, Barbour AG, Hayes SF, Benach JL, Grunwaldt E, Davis JP (1982) Lyme disease-a tick-borne spirochetosis? Science; 216: 1317-9

33. Canales M, Almazan C, Naranjo V, Jongejan F, de la Fuente J (2009) Vaccination with recombinant Boophilus annulatus Bm86 ortholog protein, Ba86, protects cattle against B. annulatus and B. microplus infestations. BMC Biotechnol; 9: 29

34. Cao W, Henry MD, Borrow P, Yamada H, Elder JH, Ravkov EV, et al. (1998) Identification of alpha-dystroglycan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus. Science; 282: 2079-81

35. Carlson D, Hernandez J, Bloom BJ, Coburn J, Aversa JM, Steere AC (1999) Lack of Borrelia burgdorferi DNA in synovial samples from patients with antibiotic treatment-resistant Lyme arthritis. Arthritis Rheum; 42: 2705-9

36. Carlsson SA, Granlund H, Nyman D, Wahlberg P (1998) IgG seroprevalence of Lyme borreliosis in the population of the Aland Islands in Finland. Scand J Infect Dis; 30: 501-3

37. Cerar D, Cerar T, Ruzic-Sabljic E, Wormser GP, Strle F (2010) Subjective symptoms after treatment of early Lyme disease. Am J Med; 123: 79-86

38. Cerar T, Ogrinc K, Cimperman J, Lotric-Furlan S, Strle F, Ruzic-Sabljic E (2008) Validation of cultivation and PCR methods for diagnosis of Lyme neuroborreliosis. J Clin Microbiol; 46: 3375-9

39. Cerar T, Ruzic-Sabljic E, Glinsek U, Zore A, Strle F (2008) Comparison of PCR methods and culture for the detection of Borrelia spp. in patients with erythema migrans. Clin Microbiol Infect; 14: 653-8

40. Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD (2006) Isolating and engineering human antibodies using yeast surface display. Nature protocols; 1: 755-68

41. Cho YK, Shusta EV (2010) Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources. Protein engineering, design & selection : PEDS; 23: 567-77

42. Coburn J, Fischer JR, Leong JM (2005) Solving a sticky problem: new genetic approaches to

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adhesins. Trends Microbiol; 21: 372-9 44. Codolo G, Amedei A, Steere AC, Papinutto E, Cappon A, Polenghi A, et al. (2008) Borrelia

burgdorferi NapA-driven Th17 cell inflammation in lyme arthritis. Arthritis Rheum; 58: 3609-17 45. Constantin B (2014) Dystrophin complex functions as a scaffold for signalling proteins.

Biochim Biophys Acta; 1838: 635-42 46. Cottle LE, Mekonnen E, Beadsworth MB, Miller AR, Beeching NJ (2012) Lyme disease in a

British referral clinic. QJM : monthly journal of the Association of Physicians; 105: 537-43 47. Coumou J, Herkes EA, Brouwer MC, van de Beek D, Tas SW, Casteelen G, et al. (2015) Ticking

the right boxes: classification of patients suspected of Lyme borreliosis at an academic referral center in the Netherlands. Clin Microbiol Infect; 21: 368.e11-20

48. Coumou J, Hovius JW, van Dam AP (2014) Borrelia burgdorferi sensu lato serology in the Netherlands: guidelines versus daily practice. Eur J Clin Microbiol Infect Dis; 33: 1803-8

49. Coumou J, van der Poll T, Speelman P, Hovius JW (2011) Tired of Lyme borreliosis. Lyme borreliosis in the Netherlands. Neth J Med; 69: 101-11

50. Couvreur B, Beaufays J, Charon C, Lahaye K, Gensale F, Denis V, et al. (2008) Variability and action mechanism of a family of anticomplement proteins in Ixodes ricinus. PloS one; 3: e1400

51. Curran KL, Fish D, Piesman J (1993) Reduction of nymphal Ixodes dammini (Acari: Ixodidae) in a residential suburban landscape by area application of insecticides. J Med Entomol; 30: 107-13

52. Dai J, Narasimhan S, Zhang L, Liu L, Wang P, Fikrig E (2010) Tick histamine release factor is critical for Ixodes scapularis engorgement and transmission of the lyme disease agent. PLoS Pathog; 6: e1001205

53. Dai J, Wang P, Adusumilli S, Booth CJ, Narasimhan S, Anguita J, et al. (2009) Antibodies against a tick protein, Salp15, protect mice from the Lyme disease agent. Cell Host Microbe; 6: 482-92

54. Daix V, Schroeder H, Praet N, Georgin JP, Chiappino I, Gillet L, et al. (2007) Ixodes ticks belonging to the Ixodes ricinus complex encode a family of anticomplement proteins. Insect molecular biology; 16: 155-66

55. Das S, Banerjee G, DePonte K, Marcantonio N, Kantor FS, Fikrig E (2001) Salp25D, an Ixodes scapularis antioxidant, is 1 of 14 immunodominant antigens in engorged tick salivary glands. J Infect Dis; 184: 1056-64

56. Dattwyler RJ, Halperin JJ (1987) Failure of tetracycline therapy in early Lyme disease. Arthritis Rheum; 30: 448-50

57. Dattwyler RJ, Luft BJ, Kunkel MJ, Finkel MF, Wormser GP, Rush TJ, et al. (1997) Ceftriaxone compared with doxycycline for the treatment of acute disseminated Lyme disease. N Engl J Med; 337: 289-94

58. de la Fuente J, Almazan C, Canales M, Perez de la Lastra JM, Kocan KM, Willadsen P (2007) A ten-year review of commercial vaccine performance for control of tick infestations on cattle. Animal health research reviews / Conference of Research Workers in Animal Diseases; 8: 23-8

59. de la Fuente J, Estrada-Pena A, Venzal JM, Kocan KM, Sonenshine DE (2008) Overview: Ticks as vectors of pathogens that cause disease in humans and animals. Front Biosci; 13: 6938-46

60. de la Fuente J, Kocan KM, Almazan C, Blouin EF (2008) Targeting the tick-pathogen interface for novel control strategies. Frontiers in bioscience : a journal and virtual library; 13: 6947-56

61. De Silva AM, Fikrig E (1995) Growth and migration of Borrelia burgdorferi in Ixodes ticks during blood feeding. The American journal of tropical medicine and hygiene; 53: 397-404.

62. de Taeye SW, Kreuk L, van Dam AP, Hovius JW, Schuijt TJ (2013) Complement evasion by Borrelia burgdorferi: it takes three to tango. Trends Parasitol; 29: 119-28

63. de Vos S, Zeinstra L, Taoufik O, Willadsen P, Jongejan F (2001) Evidence for the utility of the Bm86 antigen from Boophilus microplus in vaccination against other tick species. Exp Appl Acarol; 25: 245-61

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105. Hofhuis A, Harms M, Bennema S, van den Wijngaard CC, van Pelt W (2015) Physician reported incidence of early and late Lyme borreliosis. Parasites & vectors; 8: 161

106. Hofhuis A, Harms MG, Giessen JWBvd, Sprong H, Notermans DW, Pelt Wv (2010) Ziekte van Lyme in Nederland 1994-2009: Aantal huisartsconsulten blijft toenemen. Is voorlichting en curatief beleid genoeg? . Infectieziekten Bulletin Jaargang 21 nummer 03 21: 84-7

107. Hofhuis A, van der Giessen JW, Borgsteede FH, Wielinga PR, Notermans DW, van Pelt W (2006) Lyme borreliosis in the Netherlands: strong increase in GP consultations and hospital admissions in past 10 years. Euro Surveill; 11: E060622 2

108. Hovius JW (2009) Spitting image: tick saliva assists the causative agent of Lyme disease in evading host skin's innate immune response. J Invest Dermatol; 129: 2337-9

109. Hovius JW, Bijlsma MF, van der Windt GJ, Wiersinga WJ, Boukens BJ, Coumou J, et al. (2009) The urokinase receptor (uPAR) facilitates clearance of Borrelia burgdorferi. PLoS Pathog; 5: e1000447

110. Hovius JW, Levi M, Fikrig E (2008) Salivating for knowledge: potential pharmacological agents in tick saliva. PLoS Med; 5: e43

111. Hovius JW, Ramamoorthi N, Van't Veer C, de Groot KA, Nijhof AM, Jongejan F, et al. (2007) Identification of Salp15 homologues in Ixodes ricinus ticks. Vector Borne Zoonotic Dis; 7: 296-303

112. Hovius JW, Schuijt TJ, de Groot KA, Roelofs JJ, Oei GA, Marquart JA, et al. (2008) Preferential protection of Borrelia burgdorferi sensu stricto by a Salp15 homologue in Ixodes ricinus saliva. J Infect Dis; 198: 1189-97

113. Hovius JW, Speelman P (2012) Chronic Lyme disease: a confusing entity. Tijdschrift voor Infectieziekten; 7: 20-9

114. Hovius JW, van Dam AP, Fikrig E (2007) Tick-host-pathogen interactions in Lyme borreliosis. Trends Parasitol; 23: 434-8

115. Hovius JWR, van Dam AP, Fikrig E. Late Manifestations of Lyme Borreliosis. In: Fratamico PM, Smith JL, Brogden KA, editors. Sequela and Long-Term Consequences of Infectious Diseases. Washington DC: ASM Press; 2009.

116. Huppertz HI, Bohme M, Standaert SM, Karch H, Plotkin SA (1999) Incidence of Lyme borreliosis in the Wurzburg region of Germany. Eur J Clin Microbiol Infect Dis; 18: 697-703

117. Ibraghimov-Beskrovnaya O, Ervasti JM, Leveille CJ, Slaughter CA, Sernett SW, Campbell KP (1992) Primary structure of dystrophin-associated glycoproteins linking dystrophin to the extracellular matrix. Nature; 355: 696-702

118. Ip WK, Takahashi K, Ezekowitz RA, Stuart LM (2009) Mannose-binding lectin and innate immunity. Immunological reviews; 230: 9-21

119. Ip WK, Takahashi K, Moore KJ, Stuart LM, Ezekowitz RA (2008) Mannose-binding lectin enhances Toll-like receptors 2 and 6 signaling from the phagosome. J Exp Med; 205: 169-81

120. Jacobs JJ, Noordhoek GT, Brouwers JM, Wielinga PR, Jacobs JP, Brandenburg AH (2008) Small risk of developing Lyme borreliosis following a tick bite on Ameland: research in a general practice. Ned Tijdschr Geneeskd; 152: 2022-6

121. Jones KL, McHugh GA, Glickstein LJ, Steere AC (2009) Analysis of Borrelia burgdorferi genotypes in patients with Lyme arthritis: High frequency of ribosomal RNA intergenic spacer type 1 strains in antibiotic-refractory arthritis. Arthritis Rheum; 60: 2174-82

122. Kaiser R (1998) Neuroborreliosis. J Neurol; 245: 247-55 123. Kannian P, McHugh G, Johnson BJ, Bacon RM, Glickstein LJ, Steere AC (2007) Antibody

responses to Borrelia burgdorferi in patients with antibiotic-refractory, antibiotic-responsive, or non-antibiotic-treated Lyme arthritis. Arthritis Rheum; 56: 4216-25

124. Kaplan RF, Trevino RP, Johnson GM, Levy L, Dornbush R, Hu LT, et al. (2003) Cognitive

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function in post-treatment Lyme disease: do additional antibiotics help? Neurology; 60: 1916-22 125. Karlsson M, Hovind-Hougen K, Svenungsson B, Stiernstedt G (1990) Cultivation and

characterization of spirochetes from cerebrospinal fluid of patients with Lyme borreliosis. J Clin Microbiol; 28: 473-9

126. Kazimirova M, Stibraniova I (2013) Tick salivary compounds: their role in modulation of host defences and pathogen transmission. Front Cell Infect Microbiol; 3: 43

127. Keizer MP, Wouters D, Schlapbach LJ, Kuijpers TW (2014) Restoration of MBL-deficiency: redefining the safety, efficacy and viability of MBL-substitution therapy. Mol Immunol; 61: 174-84

128. Kelley LA, Sternberg MJ (2009) Protein structure prediction on the Web: a case study using the Phyre server. Nature protocols; 4: 363-71

129. Kishore U, Reid KB (2000) C1q: structure, function, and receptors. Immunopharmacology; 49: 159-70

130. Klempner MS (2002) Controlled trials of antibiotic treatment in patients with post-treatment chronic Lyme disease. Vector Borne Zoonotic Dis; 2: 255-63

131. Klempner MS, Hu LT, Evans J, Schmid CH, Johnson GM, Trevino RP, et al. (2001) Two controlled trials of antibiotic treatment in patients with persistent symptoms and a history of Lyme disease. N Engl J Med; 345: 85-92

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231. Schuijt TJ, Coumou J, Narasimhan S, Dai J, Deponte K, Wouters D, et al. (2011) A tick mannose-binding lectin inhibitor interferes with the vertebrate complement cascade to enhance transmission of the lyme disease agent. Cell host & microbe; 10: 136-46

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233. Schuijt TJ, Hovius JW, van der Poll T, van Dam AP, Fikrig E (2011) Lyme borreliosis vaccination: the facts, the challenge, the future. Trends Parasitol; 27: 40-7

234. Schuijt TJ, Narasimhan S, Daffre S, DePonte K, Hovius JW, Van't Veer C, et al. (2011) Identification and characterization of Ixodes scapularis antigens that elicit tick immunity using yeast surface display. PloS one; 6: e15926

235. Schuijt TJ, Narasimhan S, Daffre S, Deponte K, Hovius JW, Veer CV, et al. (2011) Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display. PLoS One; 6: e15926

236. Schultz J, Milpetz F, Bork P, Ponting CP (1998) SMART, a simple modular architecture research tool: identification of signaling domains. Proc Natl Acad Sci U S A; 95: 5857-64

237. Schwan TG, Piesman J (2002) Vector interactions and molecular adaptations of lyme disease and relapsing fever spirochetes associated with transmission by ticks. Emerging infectious diseases; 8: 115-21

238. Schwan TG, Piesman J, Golde WT, Dolan MC, Rosa PA (1995) Induction of an outer surface protein on Borrelia burgdorferi during tick feeding. Proc Natl Acad Sci U S A; 92: 2909-13

239. Senel M, Rupprecht TA, Tumani H, Pfister HW, Ludolph AC, Brettschneider J (2010) The chemokine CXCL13 in acute neuroborreliosis. J Neurol Neurosurg Psychiatry; 81: 929-33

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242. Shen S, Shin JJ, Strle K, McHugh G, Li X, Glickstein LJ, et al. (2010) Treg cell numbers and function in patients with antibiotic-refractory or antibiotic-responsive Lyme arthritis. Arthritis Rheum; 62: 2127-37 243. Shi L, Takahashi K, Dundee J, Shahroor-Karni S, Thiel S, Jensenius JC, et al. (2004) Mannose-binding lectin-deficient mice are susceptible to infection with Staphylococcus aureus. J Exp Med; 199: 1379-90

244. Shoemaker RC, Giclas PC, Crowder C, House D, Glovsky MM (2008) Complement split products C3a and C4a are early markers of acute lyme disease in tick bite patients in the United States. Int Arch Allergy Immunol; 146: 255-61

245. Sigal LH (1996) Lyme disease overdiagnosis: causes and cure. Hospital practice; 31: 13-5, 20, 6 passim

246. Sigal LH, Hassett AL (2002) Contributions of societal and geographical environments to "chronic Lyme disease": the psychopathogenesis and aporology of a new "medically unexplained symptoms" syndrome. Environ Health Perspect; 110 Suppl 4: 607-11

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248. Sood SK, Salzman MB, Johnson BJ, Happ CM, Feig K, Carmody L, et al. (1997) Duration of tick attachment as a predictor of the risk of Lyme disease in an area in which Lyme disease is endemic. J Infect Dis; 175: 996-9

249. Speelman P, de Jongh BM, Wolfs TF, Wittenberg J (2004) [Guideline 'Lyme borreliosis']. Ned Tijdschr Geneeskd; 148: 659-63

250. Spence HJ, Dhillon AS, James M, Winder SJ (2004) Dystroglycan, a scaffold for the ERK-MAP kinase cascade. EMBO reports; 5: 484-9

251. Sprong H, Trentelman J, Seemann I, Grubhoffer L, Rego RO, Hajdusek O, et al. (2014) ANTIDotE: anti-tick vaccines to prevent tick-borne diseases in Europe. Parasites & vectors; 7: 77

252. Stanek G, Breier F, Menzinger G, Schaar B, Hafner M, Partsch H (1999) Erythema migrans and serodiagnosis by enzyme immunoassay and immunoblot with three Borrelia species. Wien Klin Wochenschr; 111: 951-6

253. Stanek G, Fingerle V, Hunfeld KP, Jaulhac B, Kaiser R, Krause A, et al. (2011) Lyme borreliosis: clinical case definitions for diagnosis and management in Europe. Clin Microbiol Infect; 17: 69-79

254. Stanek G, O'Connell S, Cimmino M, Aberer E, Kristoferitsch W, Granstrom M, et al. (1996) European Union Concerted Action on Risk Assessment in Lyme Borreliosis: clinical case definitions for Lyme borreliosis. Wien Klin Wochenschr; 108: 741-7

255. Stanek G, Strle F (2009) Lyme borreliosis: a European perspective on diagnosis and clinical management. Current opinion in infectious diseases; 22: 450-4

256. Stanek G, Wormser GP, Gray J, Strle F (2012) Lyme borreliosis. Lancet; 379: 461-73 257. Steere AC (1989) Lyme disease. N Engl J Med; 321: 586-96 258. Steere AC (2001) Lyme disease. N Engl J Med; 345: 115-25 259. Steere AC, Coburn J, Glickstein L (2004) The emergence of Lyme disease. J Clin Invest; 113:

1093-101 260. Steere AC, Malawista SE, Snydman DR, Shope RE, Andiman WA, Ross MR, et al. (1977) Lyme

arthritis: an epidemic of oligoarticular arthritis in children and adults in three connecticut communities. Arthritis Rheum; 20: 7-17

261. Steere AC, McHugh G, Damle N, Sikand VK (2008) Prospective study of serologic tests for lyme disease. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America; 47: 188-95

262. Steere AC, Schoen RT, Taylor E (1987) The clinical evolution of Lyme arthritis. Ann Intern Med; 107: 725-31

263. Steere AC, Taylor E, McHugh GL, Logigian EL (1993) The overdiagnosis of Lyme disease. JAMA : the journal of the American Medical Association; 269: 1812-6

264. Straubinger RK, Summers BA, Chang YF, Appel MJ (1997) Persistence of Borrelia burgdorferi in experimentally infected dogs after antibiotic treatment. J Clin Microbiol; 35: 111-6

265. Stricker RB, Savely VR, Motanya NC, Giclas PC (2009) Complement split products c3a and c4a in chronic lyme disease. Scand J Immunol; 69: 64-9

266. Stricker RB, Winger EE (2001) Decreased CD57 lymphocyte subset in patients with chronic Lyme disease. Immunol Lett; 76: 43-8

267. Strle F, Nadelman RB, Cimperman J, Nowakowski J, Picken RN, Schwartz I, et al. (1999) Comparison of culture-confirmed erythema migrans caused by Borrelia burgdorferi sensu stricto in New York State and by Borrelia afzelii in Slovenia. Ann Intern Med; 130: 32-6

268. Strle F, Pleterski-Rigler D, Stanek G, Pejovnik-Pustinek A, Ruzic E, Cimperman J (1992) Solitary Borrelial lymphocytoma: report of 36 cases. Infection; 20: 201-6

269. Strle F, Stanek G (2009) Clinical manifestations and diagnosis of lyme borreliosis. Curr Probl Dermatol; 37: 51-110

270. Strle K, Shin JJ, Glickstein L, Steere AC (2010) Toll-Like Receptor 1 Polymorphism (1805GG) Is

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complication. Expert Rev Anti Infect Ther; 9: 1179-90 272. Takahashi M, Ishida Y, Iwaki D, Kanno K, Suzuki T, Endo Y, et al. (2010) Essential role of

mannose-binding lectin-associated serine protease-1 in activation of the complement factor D. J Exp Med; 207: 29-37

273. Takken W, Vliet AJHv, Overbeek Lv, Gassner F, Jacobs F, Bron WA, et al. (2008) Teken, tekenbeten en Borrelia infecties in Nederland Deel II. Wageningen UR, Wageningen:

274. Tillotson BJ, Cho YK, Shusta EV (2013) Cells and cell lysates: a direct approach for engineering antibodies against membrane proteins using yeast surface display. Methods; 60: 27-37

275. Trager W (1939) Acquired immunity to ticks. J Parasitol; 7: 15-20 276. Tumani H, Nolker G, Reiber H (1995) Relevance of cerebrospinal fluid variables for early

diagnosis of neuroborreliosis. Neurology; 45: 1663-70 277. Tyson K, Elkins C, Patterson H, Fikrig E, de Silva A (2007) Biochemical and functional

characterization of Salp20, an Ixodes scapularis tick salivary protein that inhibits the complement pathway. Insect Mol Biol; 16: 469-79

278. Tyson KR, Elkins C, de Silva AM (2008) A novel mechanism of complement inhibition unmasked by a tick salivary protein that binds to properdin. Journal of immunology; 180: 3964-8

279. Valenzuela JG, Charlab R, Mather TN, Ribeiro JM (2000) Purification, cloning, and expression of a novel salivary anticomplement protein from the tick, Ixodes scapularis. J Biol Chem; 275: 18717-23

280. van Burgel ND, Kroes ACM, van Dam AP (2010) CXCL13 in CSF as a biomarker for diagnosing Lyme neuroborreliosis. Abstract:

281. van Dam AP (2002) Diversity of Ixodes-borne Borrelia species--clinical, pathogenetic, and diagnostic implications and impact on vaccine development. Vector Borne Zoonotic Dis; 2: 249-54

282. van Dam AP, Kuiper H, Vos K, Widjojokusumo A, de Jongh BM, Spanjaard L, et al. (1993) Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis. Clin Infect Dis; 17: 708-17

283. van Dam AP, Oei A, Jaspars R, Fijen C, Wilske B, Spanjaard L, et al. (1997) Complement-mediated serum sensitivity among spirochetes that cause Lyme disease. Infection and immunity; 65: 1228-36

284. van den Wijngaard CC, Hofhuis A, Harms MG, Haagsma JA, Wong A, de Wit GA, et al. (2015) The burden of Lyme borreliosis expressed in disability-adjusted life years. European journal of public health:

285. van der Windt GJ, van 't Veer C, Florquin S, van der Poll T (2010) CD44 deficiency is associated with enhanced Escherichia coli-induced proinflammatory cytokine and chemokine release by peritoneal macrophages. Infect Immun; 78: 115-24

286. Vanousova D, Hercogova J (2008) Lyme borreliosis treatment. Dermatologic therapy; 21: 101-9 287. Verdu P, Barreiro LB, Patin E, Gessain A, Cassar O, Kidd JR, et al. (2006) Evolutionary

insights into the high worldwide prevalence of MBL2 deficiency alleles. Human molecular genetics; 15: 2650-8

288. von Stedingk LV, Olsson I, Hanson HS, Asbrink E, Hovmark A (1995) Polymerase chain reaction for detection of Borrelia burgdorferi DNA in skin lesions of early and late Lyme borreliosis. Eur J Clin Microbiol Infect Dis; 14: 1-5

289. Wagemakers A, Mason LM, Oei A, de Wever B, van der Poll T, Bins AD, et al. (2014) Rapid outer-surface protein C DNA tattoo vaccination protects against Borrelia afzelii infection. Gene therapy; 21: 1051-7

290. Wagemakers A, Oei A, Fikrig MM, Miellet WR, Hovius JW (2014) The relapsing fever spirochete Borrelia miyamotoi is cultivable in a modified Kelly-Pettenkofer medium, and is resistant to human complement. Parasites & vectors; 7: 418

References

230

291. Warshafsky S, Lee DH, Francois LK, Nowakowski J, Nadelman RB, Wormser GP (2010) Efficacy of antibiotic prophylaxis for the prevention of Lyme disease: an updated systematic review and meta-analysis. J Antimicrob Chemother; 65: 1137-44

292. Weber K, Preac-Mursic V, Wilske B, Thurmayr R, Neubert U, Scherwitz C (1990) A randomized trial of ceftriaxone versus oral penicillin for the treatment of early European Lyme borreliosis. Infection; 18: 91-6

293. Wikel S (2013) Ticks and tick-borne pathogens at the cutaneous interface: host defenses, tick countermeasures, and a suitable environment for pathogen establishment. Frontiers in microbiology; 4: 337

294. Willadsen P, Riding GA, McKenna RV, Kemp DH, Tellam RL, Nielsen JN, et al. (1989) Immunologic control of a parasitic arthropod. Identification of a protective antigen from Boophilus microplus. J Immunol; 143: 1346-51

295. Wilske B (2005) Epidemiology and diagnosis of Lyme borreliosis. Ann Med; 37: 568-79 296. Wilske B, Fingerle V, Schulte-Spechtel U (2007) Microbiological and serological diagnosis of

Lyme borreliosis. FEMS Immunol Med Microbiol; 49: 13-21 297. Wormser GP, Bittker S, Cooper D, Nowakowski J, Nadelman RB, Pavia C (2001) Yield of

large-volume blood cultures in patients with early Lyme disease. J Infect Dis; 184: 1070-2 298. Wormser GP, Dattwyler RJ, Shapiro ED, Halperin JJ, Steere AC, Klempner MS, et al. (2006)

The clinical assessment, treatment, and prevention of lyme disease, human granulocytic anaplasmosis, and babesiosis: clinical practice guidelines by the Infectious Diseases Society of America. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America; 43: 1089-134

299. Wormser GP, Liveris D, Hanincova K, Brisson D, Ludin S, Stracuzzi VJ, et al. (2008) Effect of Borrelia burgdorferi genotype on the sensitivity of C6 and 2-tier testing in North American patients with culture-confirmed Lyme disease. Clin Infect Dis; 47: 910-4

300. Wormser GP, Ramanathan R, Nowakowski J, McKenna D, Holmgren D, Visintainer P, et al. (2003) Duration of antibiotic therapy for early Lyme disease. A randomized, double-blind, placebo-controlled trial. Ann Intern Med; 138: 697-704

301. Wormser GP, Schwartz I (2009) Antibiotic treatment of animals infected with Borrelia burgdorferi. Clin Microbiol Rev; 22: 387-95

302. Wouters D, Wagenaar-Bos I, van Ham M, Zeerleder S (2008) C1 inhibitor: just a serine protease inhibitor? New and old considerations on therapeutic applications of C1 inhibitor. Expert opinion on biological therapy; 8: 1225-40

303. Wressnigg N, Barrett PN, Pollabauer EM, O'Rourke M, Portsmouth D, Schwendinger MG, et al. (2014) A Novel multivalent OspA vaccine against Lyme borreliosis is safe and immunogenic in an adult population previously infected with Borrelia burgdorferi sensu lato. Clin Vaccine Immunol; 21: 1490-9

304. Wressnigg N, Pollabauer EM, Aichinger G, Portsmouth D, Low-Baselli A, Fritsch S, et al. (2013) Safety and immunogenicity of a novel multivalent OspA vaccine against Lyme borreliosis in healthy adults: a double-blind, randomised, dose-escalation phase 1/2 trial. The Lancet infectious diseases; 13: 680-9

305. Yang X, Lenhart TR, Kariu T, Anguita J, Akins DR, Pal U (2010) Characterization of unique regions of Borrelia burgdorferi surface-located membrane protein 1. Infect Immun:

306. Yang XF, Pal U, Alani SM, Fikrig E, Norgard MV (2004) Essential role for OspA/B in the life cycle of the Lyme disease spirochete. J Exp Med; 199: 641-8

307. Zhang L, Zhang Y, Adusumilli S, Liu L, Narasimhan S, Dai J, et al. (2011) Molecular interactions that enable movement of the Lyme disease agent from the tick gut into the hemolymph. PLoS Pathog; 7: e1002079

References

229

a Risk Factor for Antibiotic-Refractory Lyme arthritis. Abstract: 271. Takahashi K (2011) Mannose-binding lectin and the balance between immune protection and

complication. Expert Rev Anti Infect Ther; 9: 1179-90 272. Takahashi M, Ishida Y, Iwaki D, Kanno K, Suzuki T, Endo Y, et al. (2010) Essential role of

mannose-binding lectin-associated serine protease-1 in activation of the complement factor D. J Exp Med; 207: 29-37

273. Takken W, Vliet AJHv, Overbeek Lv, Gassner F, Jacobs F, Bron WA, et al. (2008) Teken, tekenbeten en Borrelia infecties in Nederland Deel II. Wageningen UR, Wageningen:

274. Tillotson BJ, Cho YK, Shusta EV (2013) Cells and cell lysates: a direct approach for engineering antibodies against membrane proteins using yeast surface display. Methods; 60: 27-37

275. Trager W (1939) Acquired immunity to ticks. J Parasitol; 7: 15-20 276. Tumani H, Nolker G, Reiber H (1995) Relevance of cerebrospinal fluid variables for early

diagnosis of neuroborreliosis. Neurology; 45: 1663-70 277. Tyson K, Elkins C, Patterson H, Fikrig E, de Silva A (2007) Biochemical and functional

characterization of Salp20, an Ixodes scapularis tick salivary protein that inhibits the complement pathway. Insect Mol Biol; 16: 469-79

278. Tyson KR, Elkins C, de Silva AM (2008) A novel mechanism of complement inhibition unmasked by a tick salivary protein that binds to properdin. Journal of immunology; 180: 3964-8

279. Valenzuela JG, Charlab R, Mather TN, Ribeiro JM (2000) Purification, cloning, and expression of a novel salivary anticomplement protein from the tick, Ixodes scapularis. J Biol Chem; 275: 18717-23

280. van Burgel ND, Kroes ACM, van Dam AP (2010) CXCL13 in CSF as a biomarker for diagnosing Lyme neuroborreliosis. Abstract:

281. van Dam AP (2002) Diversity of Ixodes-borne Borrelia species--clinical, pathogenetic, and diagnostic implications and impact on vaccine development. Vector Borne Zoonotic Dis; 2: 249-54

282. van Dam AP, Kuiper H, Vos K, Widjojokusumo A, de Jongh BM, Spanjaard L, et al. (1993) Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis. Clin Infect Dis; 17: 708-17

283. van Dam AP, Oei A, Jaspars R, Fijen C, Wilske B, Spanjaard L, et al. (1997) Complement-mediated serum sensitivity among spirochetes that cause Lyme disease. Infection and immunity; 65: 1228-36

284. van den Wijngaard CC, Hofhuis A, Harms MG, Haagsma JA, Wong A, de Wit GA, et al. (2015) The burden of Lyme borreliosis expressed in disability-adjusted life years. European journal of public health:

285. van der Windt GJ, van 't Veer C, Florquin S, van der Poll T (2010) CD44 deficiency is associated with enhanced Escherichia coli-induced proinflammatory cytokine and chemokine release by peritoneal macrophages. Infect Immun; 78: 115-24

286. Vanousova D, Hercogova J (2008) Lyme borreliosis treatment. Dermatologic therapy; 21: 101-9 287. Verdu P, Barreiro LB, Patin E, Gessain A, Cassar O, Kidd JR, et al. (2006) Evolutionary

insights into the high worldwide prevalence of MBL2 deficiency alleles. Human molecular genetics; 15: 2650-8

288. von Stedingk LV, Olsson I, Hanson HS, Asbrink E, Hovmark A (1995) Polymerase chain reaction for detection of Borrelia burgdorferi DNA in skin lesions of early and late Lyme borreliosis. Eur J Clin Microbiol Infect Dis; 14: 1-5

289. Wagemakers A, Mason LM, Oei A, de Wever B, van der Poll T, Bins AD, et al. (2014) Rapid outer-surface protein C DNA tattoo vaccination protects against Borrelia afzelii infection. Gene therapy; 21: 1051-7

290. Wagemakers A, Oei A, Fikrig MM, Miellet WR, Hovius JW (2014) The relapsing fever spirochete Borrelia miyamotoi is cultivable in a modified Kelly-Pettenkofer medium, and is resistant to human complement. Parasites & vectors; 7: 418

References

230

291. Warshafsky S, Lee DH, Francois LK, Nowakowski J, Nadelman RB, Wormser GP (2010) Efficacy of antibiotic prophylaxis for the prevention of Lyme disease: an updated systematic review and meta-analysis. J Antimicrob Chemother; 65: 1137-44

292. Weber K, Preac-Mursic V, Wilske B, Thurmayr R, Neubert U, Scherwitz C (1990) A randomized trial of ceftriaxone versus oral penicillin for the treatment of early European Lyme borreliosis. Infection; 18: 91-6

293. Wikel S (2013) Ticks and tick-borne pathogens at the cutaneous interface: host defenses, tick countermeasures, and a suitable environment for pathogen establishment. Frontiers in microbiology; 4: 337

294. Willadsen P, Riding GA, McKenna RV, Kemp DH, Tellam RL, Nielsen JN, et al. (1989) Immunologic control of a parasitic arthropod. Identification of a protective antigen from Boophilus microplus. J Immunol; 143: 1346-51

295. Wilske B (2005) Epidemiology and diagnosis of Lyme borreliosis. Ann Med; 37: 568-79 296. Wilske B, Fingerle V, Schulte-Spechtel U (2007) Microbiological and serological diagnosis of

Lyme borreliosis. FEMS Immunol Med Microbiol; 49: 13-21 297. Wormser GP, Bittker S, Cooper D, Nowakowski J, Nadelman RB, Pavia C (2001) Yield of

large-volume blood cultures in patients with early Lyme disease. J Infect Dis; 184: 1070-2 298. Wormser GP, Dattwyler RJ, Shapiro ED, Halperin JJ, Steere AC, Klempner MS, et al. (2006)

The clinical assessment, treatment, and prevention of lyme disease, human granulocytic anaplasmosis, and babesiosis: clinical practice guidelines by the Infectious Diseases Society of America. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America; 43: 1089-134

299. Wormser GP, Liveris D, Hanincova K, Brisson D, Ludin S, Stracuzzi VJ, et al. (2008) Effect of Borrelia burgdorferi genotype on the sensitivity of C6 and 2-tier testing in North American patients with culture-confirmed Lyme disease. Clin Infect Dis; 47: 910-4

300. Wormser GP, Ramanathan R, Nowakowski J, McKenna D, Holmgren D, Visintainer P, et al. (2003) Duration of antibiotic therapy for early Lyme disease. A randomized, double-blind, placebo-controlled trial. Ann Intern Med; 138: 697-704

301. Wormser GP, Schwartz I (2009) Antibiotic treatment of animals infected with Borrelia burgdorferi. Clin Microbiol Rev; 22: 387-95

302. Wouters D, Wagenaar-Bos I, van Ham M, Zeerleder S (2008) C1 inhibitor: just a serine protease inhibitor? New and old considerations on therapeutic applications of C1 inhibitor. Expert opinion on biological therapy; 8: 1225-40

303. Wressnigg N, Barrett PN, Pollabauer EM, O'Rourke M, Portsmouth D, Schwendinger MG, et al. (2014) A Novel multivalent OspA vaccine against Lyme borreliosis is safe and immunogenic in an adult population previously infected with Borrelia burgdorferi sensu lato. Clin Vaccine Immunol; 21: 1490-9

304. Wressnigg N, Pollabauer EM, Aichinger G, Portsmouth D, Low-Baselli A, Fritsch S, et al. (2013) Safety and immunogenicity of a novel multivalent OspA vaccine against Lyme borreliosis in healthy adults: a double-blind, randomised, dose-escalation phase 1/2 trial. The Lancet infectious diseases; 13: 680-9

305. Yang X, Lenhart TR, Kariu T, Anguita J, Akins DR, Pal U (2010) Characterization of unique regions of Borrelia burgdorferi surface-located membrane protein 1. Infect Immun:

306. Yang XF, Pal U, Alani SM, Fikrig E, Norgard MV (2004) Essential role for OspA/B in the life cycle of the Lyme disease spirochete. J Exp Med; 199: 641-8

307. Zhang L, Zhang Y, Adusumilli S, Liu L, Narasimhan S, Dai J, et al. (2011) Molecular interactions that enable movement of the Lyme disease agent from the tick gut into the hemolymph. PLoS Pathog; 7: e1002079

PhD Portfolio

232

PhD Portfolio Name PhD student: J. Coumou PhD period: September 2011 – September 2015 Name PhD supervisors: Prof. dr. T. van der Poll and Prof. dr. E. Fikrig

1. PhD Training Courses Year Workload

(ECTS) The AMC world of science 2011 0.7 Basic Laboratory Safety 2011 0.4 Laboratory Animals Course 2011 3.0 Advanced Immunology 2012 2.9 Seminars, workshops and master classes Masterclass by Jos van der Meer 2012 0.2 Masterclass by Erol Fikrig 2014 0.2 FP7 Antidote Launch Meeting, Amsterdam 2014 0.2 Weekly meeting of Dep. of Infectious diseases at Yale University 2012 0.1 Ruysch Lectures 2011-2014 0.3 Monthly meetings of the Center for Experimental Molecular Medicine at the AMC

2011-2015 1

Presentations Identification of an Ixodes scapularis midgut protein involved in the migration of Borrelia during tick feeding - XII International Jena Symposium on Tick-borne Diseases, Weimar, Germany (oral)

2013 0.5

A Tick Midgut Protein Plays a Vital Role in the Migration of Borrelia to the Salivary Glands – Research Seminar, Goethe-Universität (oral)

2014 0.5

The tick midgut protein Clone19 plays a vital role in the migration of Borrelia burgdorferi from the midgut to the salivary glands during a blood meal – International Conference on Lyme Borreliosis, Boston, USA (poster)

2013 0.5

A retrospective descriptive study of the first 200 patients in a tertiary center for Lyme disease in the Netherlands – International Conference on Lyme Borreliosis, Boston, USA (poster)

2013 0.5

The role of Mannose Binding Lectin in the immune response against Borrelia burgdorferi sensu lato – International Conference on Lyme Borreliosis, Vienna, Austria (poster)

2015 0.5

Ixodes scapularis dystroglycan-like protein promotes Borrelia burgdorferi migration from the gut – International Conference on Lyme Borreliosis, Vienna, Austria (poster)

2015 0.5

PhD Portfolio

232

PhD Portfolio Name PhD student: J. Coumou PhD period: September 2011 – September 2015 Name PhD supervisors: Prof. dr. T. van der Poll and Prof. dr. E. Fikrig

1. PhD Training Courses Year Workload

(ECTS) The AMC world of science 2011 0.7 Basic Laboratory Safety 2011 0.4 Laboratory Animals Course 2011 3.0 Advanced Immunology 2012 2.9 Seminars, workshops and master classes Masterclass by Jos van der Meer 2012 0.2 Masterclass by Erol Fikrig 2014 0.2 FP7 Antidote Launch Meeting, Amsterdam 2014 0.2 Weekly meeting of Dep. of Infectious diseases at Yale University 2012 0.1 Ruysch Lectures 2011-2014 0.3 Monthly meetings of the Center for Experimental Molecular Medicine at the AMC

2011-2015 1

Presentations Identification of an Ixodes scapularis midgut protein involved in the migration of Borrelia during tick feeding - XII International Jena Symposium on Tick-borne Diseases, Weimar, Germany (oral)

2013 0.5

A Tick Midgut Protein Plays a Vital Role in the Migration of Borrelia to the Salivary Glands – Research Seminar, Goethe-Universität (oral)

2014 0.5

The tick midgut protein Clone19 plays a vital role in the migration of Borrelia burgdorferi from the midgut to the salivary glands during a blood meal – International Conference on Lyme Borreliosis, Boston, USA (poster)

2013 0.5

A retrospective descriptive study of the first 200 patients in a tertiary center for Lyme disease in the Netherlands – International Conference on Lyme Borreliosis, Boston, USA (poster)

2013 0.5

The role of Mannose Binding Lectin in the immune response against Borrelia burgdorferi sensu lato – International Conference on Lyme Borreliosis, Vienna, Austria (poster)

2015 0.5

Ixodes scapularis dystroglycan-like protein promotes Borrelia burgdorferi migration from the gut – International Conference on Lyme Borreliosis, Vienna, Austria (poster)

2015 0.5

PhD Portfolio

233

(Inter)national conferences Year Workload (ECTS)

Biology of Spirochetes, Gordon Research Conference, Ventura Beach, California, USA

2012 1.25

International Conference on Lyme Borreliosis, Boston, USA 2013 1.25 XII International Jena Symposium on Tick-borne Diseases, Weimar, Germany

2013 1.0

European Congress of Clinical Microbiology and Infectious Diseases

2014 1.0

Tick Tactics, Amsterdam 2014 0.25 International Conference on Lyme Borreliosis, Vienna, Austria 2015 1.25 Other Year Workload

(ECTS) Annual Graduate Retreat, CINIMA, Haarlem, the Netherlands 2011 0.5 Retreat Ticks and tick borne diseases group, Sint Maartenszee, the Netherlands

2012 0.5

Retreat Ticks and tick borne disease group, Xàbia, Spain 2014 0.5 Organizing committee scientific conference Tick Tactics 2014 4.0 Journal Club 2011-2015 1.0 Invited peer-reviewing for multiple journals 2012-2015 1.0

2. Teaching Tutoring Year Workload

(ECTS) Ellen Vermissen (Laboratoriumonderwijs stage) 2012 0.5 Eduard Herkens (wetenschappelijke stage Geneeskunde) 2012 0.5 Willem Miellet ( masterstage Medical Biology) 2013 2.0 Jiri Moekotte (keuze-onderwijs Geneeskunde) 2014 1.0 Floor Luken (Laboratoriumonderwijs stage) 2014 1.0 Highschool students (Profielwerkstukken) 2013-2014 0.3 Lecturing Lyme borreliosis - Basiscursus Infectieziekten en Uitheemse Pathologie, AMC

2014-2015 0.6

Ticks and tricks – PhD Course Infectious diseases, AMC 2015 0.3

3. Parameters of esteem Grants Year AMC PhD Scholarship, Graduate school, Academic Medical Center

2010

Spinoza grant, Universiteitsbeurs, University of Amsterdam 2011

Van Walree beurs, KNAW 2015

List of publications

234

List of publications Coumou J, Narasimhan S, Trentelman JJ, Wagemakers A, Koetsveld J, Ersoz JI, Oei GA, Fikrig E, Hovius JW. Ixodes scapularis dystroglycan-like protein promotes Borrelia burgdorferi migration from the gut. Journal of Mol Med. 2015 Nov [Epub ahead of print].

Wagemakers A, Coumou J, Schuijt TJ, Oei GA, Nijhof AM, Van ‘t Veer C, van der Poll T, Bins AD and Hovius JW. An Ixodes ricinus Tick Salivary Lectin Pathway Inhibitor protects Borrelia burgdorferi s.l. from human complement. In Press.

Coumou J, Wagemakers A, Trentelman JJ, Nijhof AM, Hovius JW. Vaccination against Bm86 Homologues in Rabbits Does Not Impair Ixodes ricinus Feeding or Oviposition. PLoS One. 2015 Apr 28 ;10(4):e0123495.

Coumou J†, Herkes EA†, Brouwer MC, van de Beek D, Tas SW, Casteelen G, van Vugt M, Starink MV, de Vries HJC, de Wever B, Spanjaard L, Hovius JWR. Ticking the right boxes; classification of patients suspected of Lyme borreliosis at an academic referral center in the Netherlands. Clinical Microbiology and Infection 2014.

Coumou J†, Narasimhan S†, Schuijt TJ, Boder E, Hovius JW, Fikrig E. A tick gut protein with fibronectin III domains aids Borrelia burgdorferi congregation to the gut during transmission. PLoS Pathog. 2014 Aug 7;10(8):e1004278.

Coumou J, Hovius JW, van Dam AP. Borrelia burgdorferi sensu lato serology in the Netherlands: guidelines versus daily practice. Eur J Clin Microbiol Infect Dis. 2014 Oct;33(10):1803-8.

Hovius JW, de Wever B, Sohne M, Brouwer MC, Coumou J, Wagemakers A, Oei GA, Knol H, Narasimhan S, Hodiamont CJ, Jahfari S, Pals ST, Horlings HM, Fikrig E, Sprong H and van Oers MHJ. A case of meningoencephalitis by the relapsing fever spirochete Borrelia miyamotoi in Europe. The Lancet. 2013 Aug 17;382(9892):658.

Schuijt TJ, Coumou J, Dai J, Narasimhan S, DePonte K, Wouters D, Brouwer M, Oei

A, Roelofs JJTH, van Dam AP, van der Poll T, van ’t Veer C, Fikrig E & Hovius JW. A tick mannose-binding lectin inhibits the vertebrate complement cascade to enhance transmission of the Lyme disease agent. Cell Host Microbe. 2011 Aug 18;10 (2):136-46.

Coumou J, Hovius JW. Ziekte van Lyme in Nederland. Ned Tijdschr Tandheelkd. 2011 Jun;118(6):310-6.

PhD Portfolio

233

(Inter)national conferences Year Workload (ECTS)

Biology of Spirochetes, Gordon Research Conference, Ventura Beach, California, USA

2012 1.25

International Conference on Lyme Borreliosis, Boston, USA 2013 1.25 XII International Jena Symposium on Tick-borne Diseases, Weimar, Germany

2013 1.0

European Congress of Clinical Microbiology and Infectious Diseases

2014 1.0

Tick Tactics, Amsterdam 2014 0.25 International Conference on Lyme Borreliosis, Vienna, Austria 2015 1.25 Other Year Workload

(ECTS) Annual Graduate Retreat, CINIMA, Haarlem, the Netherlands 2011 0.5 Retreat Ticks and tick borne diseases group, Sint Maartenszee, the Netherlands

2012 0.5

Retreat Ticks and tick borne disease group, Xàbia, Spain 2014 0.5 Organizing committee scientific conference Tick Tactics 2014 4.0 Journal Club 2011-2015 1.0 Invited peer-reviewing for multiple journals 2012-2015 1.0

2. Teaching Tutoring Year Workload

(ECTS) Ellen Vermissen (Laboratoriumonderwijs stage) 2012 0.5 Eduard Herkens (wetenschappelijke stage Geneeskunde) 2012 0.5 Willem Miellet ( masterstage Medical Biology) 2013 2.0 Jiri Moekotte (keuze-onderwijs Geneeskunde) 2014 1.0 Floor Luken (Laboratoriumonderwijs stage) 2014 1.0 Highschool students (Profielwerkstukken) 2013-2014 0.3 Lecturing Lyme borreliosis - Basiscursus Infectieziekten en Uitheemse Pathologie, AMC

2014-2015 0.6

Ticks and tricks – PhD Course Infectious diseases, AMC 2015 0.3

3. Parameters of esteem Grants Year AMC PhD Scholarship, Graduate school, Academic Medical Center

2010

Spinoza grant, Universiteitsbeurs, University of Amsterdam 2011

Van Walree beurs, KNAW 2015

List of publications

234

List of publications Coumou J, Narasimhan S, Trentelman JJ, Wagemakers A, Koetsveld J, Ersoz JI, Oei GA, Fikrig E, Hovius JW. Ixodes scapularis dystroglycan-like protein promotes Borrelia burgdorferi migration from the gut. Journal of Mol Med. 2015 Nov [Epub ahead of print].

Wagemakers A, Coumou J, Schuijt TJ, Oei GA, Nijhof AM, Van ‘t Veer C, van der Poll T, Bins AD and Hovius JW. An Ixodes ricinus Tick Salivary Lectin Pathway Inhibitor protects Borrelia burgdorferi s.l. from human complement. In Press.

Coumou J, Wagemakers A, Trentelman JJ, Nijhof AM, Hovius JW. Vaccination against Bm86 Homologues in Rabbits Does Not Impair Ixodes ricinus Feeding or Oviposition. PLoS One. 2015 Apr 28 ;10(4):e0123495.

Coumou J†, Herkes EA†, Brouwer MC, van de Beek D, Tas SW, Casteelen G, van Vugt M, Starink MV, de Vries HJC, de Wever B, Spanjaard L, Hovius JWR. Ticking the right boxes; classification of patients suspected of Lyme borreliosis at an academic referral center in the Netherlands. Clinical Microbiology and Infection 2014.

Coumou J†, Narasimhan S†, Schuijt TJ, Boder E, Hovius JW, Fikrig E. A tick gut protein with fibronectin III domains aids Borrelia burgdorferi congregation to the gut during transmission. PLoS Pathog. 2014 Aug 7;10(8):e1004278.

Coumou J, Hovius JW, van Dam AP. Borrelia burgdorferi sensu lato serology in the Netherlands: guidelines versus daily practice. Eur J Clin Microbiol Infect Dis. 2014 Oct;33(10):1803-8.

Hovius JW, de Wever B, Sohne M, Brouwer MC, Coumou J, Wagemakers A, Oei GA, Knol H, Narasimhan S, Hodiamont CJ, Jahfari S, Pals ST, Horlings HM, Fikrig E, Sprong H and van Oers MHJ. A case of meningoencephalitis by the relapsing fever spirochete Borrelia miyamotoi in Europe. The Lancet. 2013 Aug 17;382(9892):658.

Schuijt TJ, Coumou J, Dai J, Narasimhan S, DePonte K, Wouters D, Brouwer M, Oei

A, Roelofs JJTH, van Dam AP, van der Poll T, van ’t Veer C, Fikrig E & Hovius JW. A tick mannose-binding lectin inhibits the vertebrate complement cascade to enhance transmission of the Lyme disease agent. Cell Host Microbe. 2011 Aug 18;10 (2):136-46.

Coumou J, Hovius JW. Ziekte van Lyme in Nederland. Ned Tijdschr Tandheelkd. 2011 Jun;118(6):310-6.

List of publications

235

Coumou J, van der Poll T, Speelman P, Hovius JW. Tired of Lyme borreliosis. Neth J Med. 2011: 69;101-111.

Hovius JW, Bijlsma MF, van der Windt GJ, Wiersinga WJ, Boukens BJ, Coumou J, Oei A, de Beer R, de Vos AF, van 't Veer C, van Dam AP, Wang P, Fikrig E, Levi MM, Roelofs JJ, van der Poll T. The urokinase receptor (uPAR) facilitates clearance of Borrelia burgdorferi. PLoS Pathog. 2009: 5;e1000447. † These authors contributed equally to this work.

Curriculum vitae

236

Curriculum vitae Jeroen Coumou was born on the 24th of March 1988 in Enkhuizen, the Netherlands. After high school graduation in 2006 from the Regionale Scholen Gemeenschap in Enkhuizen he moved to Amsterdam to study Medicine at the Academic Medical Center, University of Amsterdam. In the second year of medical school he was accepted in the Honours programme and became interested in fundamental research. In 2008 he started his first research internship at the Center for Experimental Molecular Medicine (CEMM) at the AMC under supervision of dr. Joppe Hovius and prof. dr. Tom van der Poll where he became intrigued with the pathogenesis of Lyme borreliosis, in specific the role of tick proteins during Borrelia burgdorferi transmission. In 2010 he worked as a research student at the Public Health Institute in Amsterdam (Streeklaboratorium GGD Amsterdam) under supervision of dr. Alje van Dam investigating the value of serological tests for Lyme borreliosis. At the same time he studied the effect of the tick salivary protein TSLPI on Borrelia burgdorferi transmission at the CEMM under supervision of dr. Joppe W. Hovius and dr. Tim J. Schuijt. Shortly thereafter, he left for the United States where he continued his research on TSLPI under supervision of prof. Erol Fikrig and dr. Sukanya Narasimhan at Yale University, New Haven, Connecticut, USA. After his visit to the US, he returned to Amsterdam in 2011 where he obtained his Master of Science with an honours degree and he received an AMC PhD Graduate Scholarship, allowing him to become a PhD-student at CEMM under supervision of dr. Joppe Hovius and prof. dr. Tom van der Poll. In 2012 he visited Yale University for a second time to study the role of tick gut proteins during Borrelia burgdorferi transmission. Afterwards, his experience with tick-animal models in the US allowed him to set up a tick colony at the AMC in Amsterdam for scientific purposes. His broad interest in clinical aspects and fundamental research in Lyme borreliosis resulted in a dissertation with several distinct themes. He is currently doing his clinical rotations and is expected to finish medical school in 2017. He aspires to become an internal medicine doctor. Jeroen lives in Amsterdam together with his girlfriend Janneke Linnekamp.

List of publications

235

Coumou J, van der Poll T, Speelman P, Hovius JW. Tired of Lyme borreliosis. Neth J Med. 2011: 69;101-111.

Hovius JW, Bijlsma MF, van der Windt GJ, Wiersinga WJ, Boukens BJ, Coumou J, Oei A, de Beer R, de Vos AF, van 't Veer C, van Dam AP, Wang P, Fikrig E, Levi MM, Roelofs JJ, van der Poll T. The urokinase receptor (uPAR) facilitates clearance of Borrelia burgdorferi. PLoS Pathog. 2009: 5;e1000447. † These authors contributed equally to this work.

Curriculum vitae

236

Curriculum vitae Jeroen Coumou was born on the 24th of March 1988 in Enkhuizen, the Netherlands. After high school graduation in 2006 from the Regionale Scholen Gemeenschap in Enkhuizen he moved to Amsterdam to study Medicine at the Academic Medical Center, University of Amsterdam. In the second year of medical school he was accepted in the Honours programme and became interested in fundamental research. In 2008 he started his first research internship at the Center for Experimental Molecular Medicine (CEMM) at the AMC under supervision of dr. Joppe Hovius and prof. dr. Tom van der Poll where he became intrigued with the pathogenesis of Lyme borreliosis, in specific the role of tick proteins during Borrelia burgdorferi transmission. In 2010 he worked as a research student at the Public Health Institute in Amsterdam (Streeklaboratorium GGD Amsterdam) under supervision of dr. Alje van Dam investigating the value of serological tests for Lyme borreliosis. At the same time he studied the effect of the tick salivary protein TSLPI on Borrelia burgdorferi transmission at the CEMM under supervision of dr. Joppe W. Hovius and dr. Tim J. Schuijt. Shortly thereafter, he left for the United States where he continued his research on TSLPI under supervision of prof. Erol Fikrig and dr. Sukanya Narasimhan at Yale University, New Haven, Connecticut, USA. After his visit to the US, he returned to Amsterdam in 2011 where he obtained his Master of Science with an honours degree and he received an AMC PhD Graduate Scholarship, allowing him to become a PhD-student at CEMM under supervision of dr. Joppe Hovius and prof. dr. Tom van der Poll. In 2012 he visited Yale University for a second time to study the role of tick gut proteins during Borrelia burgdorferi transmission. Afterwards, his experience with tick-animal models in the US allowed him to set up a tick colony at the AMC in Amsterdam for scientific purposes. His broad interest in clinical aspects and fundamental research in Lyme borreliosis resulted in a dissertation with several distinct themes. He is currently doing his clinical rotations and is expected to finish medical school in 2017. He aspires to become an internal medicine doctor. Jeroen lives in Amsterdam together with his girlfriend Janneke Linnekamp.

Dankwoord

238

Dankwoord Het zit er bijna op. Maar niet voordat ik een aantal personen heb kunnen bedanken voor hun bijdrage aan dit boekje en mijn promotieonderzoek.

Allereerst, Joppe Hovius. Dat ik in december 2007 met mijn rugzak het Centrum voor Experimentele en Moleculaire Geneeskunde (CEMM) binnenliep voor een keuzestage bij jou – jij toen nog zelf promovendus - heb ik altijd ervaren als een fortuinlijk moment. De daaropvolgende onderzoekstages en het promotie-onderzoek waarin je mij vol enthousiasme hebt begeleid, zaten vol met waardevolle momenten en ontwikkelingen die een belangrijke bijdrage hebben geleverd aan mijn medische en wetenschappelijke carrière. Ik denk zowel aan eerste stappen zoals het leren vasthouden van een pipet en het schrijven van een review, tot grote mijlpalen zoals de AMC PhD Scholarship en het mogelijk maken van mijn onderzoek op Yale University. Ik voelde me altijd gesteund in mijn keuzes door jou en het vertrouwen en de vrijheid die je mij hebt gegeven gedurende mijn promotieonderzoek waren essentieel voor mijn ontwikkeling. Je hebt me altijd meer dan genoeg springplanken geboden om het meeste uit mijzelf te halen. Tevens was je ondanks je andere taken snel bereikbaar wanneer ik ergens vast liep – ook buiten het onderzoek. Jouw ambitie, doorzettingsvermogen, betrokkenheid en onkreukbaar optimisme zijn een inspiratie voor mij geweest. Mooi was ook om van dichtbij mee te maken hoe jij de afgelopen jaren opklom van promovendus tot Principial Investigator met een alsmaar groeiende groep promovendi. Tenslotte kijk ik terug op veel lol samen, surfen in California – that’s my wave, dude! -, roadtrippen in New England, teken vangen in Sint Maartenszee en de retraite in Jávea. De toekomst blijft hopelijk even mooie momenten brengen!

Ook wil ik mijn beide promotoren, prof. Tom van der Poll en Erol Fikrig bedanken. Tom, dank voor al je inspanningen als promotor. Dit boekje was niet tot stand gekomen zonder jouw hulp en in het bijzonder de ruimte en mogelijkheden die je Joppe hebt gegeven voor het onderzoek naar vieze teken-beesten, Lyme en speekseleiwitten binnen jouw onderzoeksgroep. Ondanks de verscheidenheid aan thema’s waar in jouw groep onderzoek naar wordt gedaan ben je altijd goed op de hoogte en bij iedereen betrokken. Erol, thanks for the opportunities to work in your lab during my PhD. Your guidance and advice in my research projects at Yale as well as in Amsterdam have been valuable. The ongoing collaboration between Yale

Dankwoord

238

Dankwoord Het zit er bijna op. Maar niet voordat ik een aantal personen heb kunnen bedanken voor hun bijdrage aan dit boekje en mijn promotieonderzoek.

Allereerst, Joppe Hovius. Dat ik in december 2007 met mijn rugzak het Centrum voor Experimentele en Moleculaire Geneeskunde (CEMM) binnenliep voor een keuzestage bij jou – jij toen nog zelf promovendus - heb ik altijd ervaren als een fortuinlijk moment. De daaropvolgende onderzoekstages en het promotie-onderzoek waarin je mij vol enthousiasme hebt begeleid, zaten vol met waardevolle momenten en ontwikkelingen die een belangrijke bijdrage hebben geleverd aan mijn medische en wetenschappelijke carrière. Ik denk zowel aan eerste stappen zoals het leren vasthouden van een pipet en het schrijven van een review, tot grote mijlpalen zoals de AMC PhD Scholarship en het mogelijk maken van mijn onderzoek op Yale University. Ik voelde me altijd gesteund in mijn keuzes door jou en het vertrouwen en de vrijheid die je mij hebt gegeven gedurende mijn promotieonderzoek waren essentieel voor mijn ontwikkeling. Je hebt me altijd meer dan genoeg springplanken geboden om het meeste uit mijzelf te halen. Tevens was je ondanks je andere taken snel bereikbaar wanneer ik ergens vast liep – ook buiten het onderzoek. Jouw ambitie, doorzettingsvermogen, betrokkenheid en onkreukbaar optimisme zijn een inspiratie voor mij geweest. Mooi was ook om van dichtbij mee te maken hoe jij de afgelopen jaren opklom van promovendus tot Principial Investigator met een alsmaar groeiende groep promovendi. Tenslotte kijk ik terug op veel lol samen, surfen in California – that’s my wave, dude! -, roadtrippen in New England, teken vangen in Sint Maartenszee en de retraite in Jávea. De toekomst blijft hopelijk even mooie momenten brengen!

Ook wil ik mijn beide promotoren, prof. Tom van der Poll en Erol Fikrig bedanken. Tom, dank voor al je inspanningen als promotor. Dit boekje was niet tot stand gekomen zonder jouw hulp en in het bijzonder de ruimte en mogelijkheden die je Joppe hebt gegeven voor het onderzoek naar vieze teken-beesten, Lyme en speekseleiwitten binnen jouw onderzoeksgroep. Ondanks de verscheidenheid aan thema’s waar in jouw groep onderzoek naar wordt gedaan ben je altijd goed op de hoogte en bij iedereen betrokken. Erol, thanks for the opportunities to work in your lab during my PhD. Your guidance and advice in my research projects at Yale as well as in Amsterdam have been valuable. The ongoing collaboration between Yale

Dankwoord

239

and the AMC has helped us greatly to implement and improve our research models at the AMC. It was also special to spend time with you and your family at the Hamptons.

Then I would like to thank my copromotor Sukanya Narasimhan. You helped my greatly in my transformation from a medical student doing labwork into a PhD student performing my own experiments. Your work with the Yeast Surface Display has been of great importance for this thesis. And thanks to you I was able to get the most out of my work during my (always too short) time at Yale. Furthermore, your kindness and warm heart has made New Haven feel like home. Your optimism and perseverance when results turned out unexpected have been an inspiration. Jai-ho!

Uiteraard gaat mijn dank uit naar de overige leden van de promotiecommissie, Jaap van Dissel, Theo Geijtenbeek, Leo Joosten, Jan Prins, Peter Speelman en Hein Sprong, voor het kritisch doornemen van mijn proefschrift. Peter Speelman, dank voor het meeschrijven aan het review over de ziekte van Lyme en voor het commentaar over het manuscript over de patiëntenpopulatie van het Amsterdams Multidisciplinair Lyme Centrum.

Een waardevol aspect van mijn promotie was de samenwerking met onderzoekers van andere instituten. Zo wil ik Alje van Dam bedanken voor het mogelijk maken van, en de begeleiding tijdens, het onderzoek bij de GGD Amsterdam naar de waarde van serologische testen. Ook wil ik Ard Nijhof van de Freie Universität in Berlijn bedanken voor de samenwerking naar het tekeneiwit Ir86. Ondanks de negatieve uitkomsten van het onderzoek heeft de samenwerking geresulteerd in een mooie publicatie.

Tim Schuijt, ik ben dankbaar voor jouw voorgaand wetenschappelijk werk en jouw begeleiding tijdens mijn wetenschappelijke stage naar TSLPI en tijdens het opstarten van mijn promotieonderzoek in het AMC. Jouw passie en enthousiasme voor wetenschap hebben aanstekelijk gewerkt. Dankzij jou heb ik ook kennis mogen maken met William, die mijn tijd in New Haven onvergetelijk heeft gemaakt!

Ik heb met plezier samengewerkt met andere afdelingen van het AMC. Het onderzoek naar de patiënten van het AMLC is tot stand gekomen dankzij medewerking van de afdelingen Interne Geneeskunde, Neurologie, Reumatologie,

Dankwoord

240

Dermatologie, Psychiatrie en Medische Microbiologie. Ook wil ik de patiënten van het AMLC danken voor het mogelijk maken van dit onderzoek. Joris Roelofs van de afdeling Pathologie, dank voor je werk in het scoren van de coupes. Het opzetten van diermodellen met Ixodes teken was niet mogelijk geweest zonder de medewerking van het ARIA personeel. Karin, Sandra en Niels, dankzij jullie hebben wij op een veilige manier onze tekenmodellen kunnen opzetten en uitvoeren.

Dan alle ‘partners in Lyme’; Lauren, Alex, Joris, Jos, Jasmin, Jeanine en vers-van-de-pers Michelle. Allen dank voor jullie prettige samenwerking de afgelopen jaren. Lauren, de beginfase van onze promotie was bijzonder leuk om samen door te maken. Veel succes met de laatste loodjes van jouw promotieonderzoek. Alex, je pakte de labvaardigheden snel op en bent uitgegroeid tot een mooie partner in Lyme. We hebben veel gelachen samen tijdens werk en daarbuiten. Je was altijd bereid mee te helpen met mijn experimenten, waarvoor veel dank! Je bent een toffe kerel en succes met de opleiding tot medisch microbioloog. Joris, dank voor je hulp met ISDLP en leuk om te zien dat je werk naar Rickettsia en B. miyamotoi vruchten begint af te werpen. Jos, je bent als bioloog uit Wageningen een goede aanvulling op de groep en ik heb erg genoten van je (sterke) verhalen. De Yeast Surface Display is bij jou in goede handen! Jasmin, het was altijd een plezier om met je te werken. Dank voor al je hulp, je bent goud voor de groep! Jeanine, het was niet makkelijk maar je hebt je weg in het lab gevonden. Laat die 4000 epjes maar komen! De Lyme onderzoekers zijn niet compleet zonder Anneke Oei van de Medische Microbiologie. Het was fijn om te weten dat je altijd voor mij klaar stond. Je bent nauwkeurig en je hebt een bijzonder goed geheugen, waar ik dankbaar gebruik van heb kunnen maken. We hebben een goede samenwerking gehad de afgelopen jaren, waarvoor dank. Behalve kweken ben je ook een ster in het bakken, de tekentaart was een mooi cadeau!

Als ik denk aan de Lymies, dan denk ik altijd aan de ‘Tommies’, de mede-promovendi van Tom van der Poll. Waar was ik geweest zonder jullie! Ik ben dankbaar voor jullie gezelligheid en steun de afgelopen jaren. Het congres in Barcelona, de retraites in Oost-Nederland, vis-op-vrijdag en lunch-om-half-twaalf zorgde ervoor dat ik altijd met plezier op F0 en later op G2 heb gezeten. Cees en Alex, het was altijd goed om te weten dat ik bij jullie terecht kon voor moleculaire of immunologische vragen. Brandon, dankzij jou heb ik meer kunnen halen uit mijn qPCR analyses. Verder wil ik Danielle, Regina en Monique bedanken voor

Dankwoord

239

and the AMC has helped us greatly to implement and improve our research models at the AMC. It was also special to spend time with you and your family at the Hamptons.

Then I would like to thank my copromotor Sukanya Narasimhan. You helped my greatly in my transformation from a medical student doing labwork into a PhD student performing my own experiments. Your work with the Yeast Surface Display has been of great importance for this thesis. And thanks to you I was able to get the most out of my work during my (always too short) time at Yale. Furthermore, your kindness and warm heart has made New Haven feel like home. Your optimism and perseverance when results turned out unexpected have been an inspiration. Jai-ho!

Uiteraard gaat mijn dank uit naar de overige leden van de promotiecommissie, Jaap van Dissel, Theo Geijtenbeek, Leo Joosten, Jan Prins, Peter Speelman en Hein Sprong, voor het kritisch doornemen van mijn proefschrift. Peter Speelman, dank voor het meeschrijven aan het review over de ziekte van Lyme en voor het commentaar over het manuscript over de patiëntenpopulatie van het Amsterdams Multidisciplinair Lyme Centrum.

Een waardevol aspect van mijn promotie was de samenwerking met onderzoekers van andere instituten. Zo wil ik Alje van Dam bedanken voor het mogelijk maken van, en de begeleiding tijdens, het onderzoek bij de GGD Amsterdam naar de waarde van serologische testen. Ook wil ik Ard Nijhof van de Freie Universität in Berlijn bedanken voor de samenwerking naar het tekeneiwit Ir86. Ondanks de negatieve uitkomsten van het onderzoek heeft de samenwerking geresulteerd in een mooie publicatie.

Tim Schuijt, ik ben dankbaar voor jouw voorgaand wetenschappelijk werk en jouw begeleiding tijdens mijn wetenschappelijke stage naar TSLPI en tijdens het opstarten van mijn promotieonderzoek in het AMC. Jouw passie en enthousiasme voor wetenschap hebben aanstekelijk gewerkt. Dankzij jou heb ik ook kennis mogen maken met William, die mijn tijd in New Haven onvergetelijk heeft gemaakt!

Ik heb met plezier samengewerkt met andere afdelingen van het AMC. Het onderzoek naar de patiënten van het AMLC is tot stand gekomen dankzij medewerking van de afdelingen Interne Geneeskunde, Neurologie, Reumatologie,

Dankwoord

240

Dermatologie, Psychiatrie en Medische Microbiologie. Ook wil ik de patiënten van het AMLC danken voor het mogelijk maken van dit onderzoek. Joris Roelofs van de afdeling Pathologie, dank voor je werk in het scoren van de coupes. Het opzetten van diermodellen met Ixodes teken was niet mogelijk geweest zonder de medewerking van het ARIA personeel. Karin, Sandra en Niels, dankzij jullie hebben wij op een veilige manier onze tekenmodellen kunnen opzetten en uitvoeren.

Dan alle ‘partners in Lyme’; Lauren, Alex, Joris, Jos, Jasmin, Jeanine en vers-van-de-pers Michelle. Allen dank voor jullie prettige samenwerking de afgelopen jaren. Lauren, de beginfase van onze promotie was bijzonder leuk om samen door te maken. Veel succes met de laatste loodjes van jouw promotieonderzoek. Alex, je pakte de labvaardigheden snel op en bent uitgegroeid tot een mooie partner in Lyme. We hebben veel gelachen samen tijdens werk en daarbuiten. Je was altijd bereid mee te helpen met mijn experimenten, waarvoor veel dank! Je bent een toffe kerel en succes met de opleiding tot medisch microbioloog. Joris, dank voor je hulp met ISDLP en leuk om te zien dat je werk naar Rickettsia en B. miyamotoi vruchten begint af te werpen. Jos, je bent als bioloog uit Wageningen een goede aanvulling op de groep en ik heb erg genoten van je (sterke) verhalen. De Yeast Surface Display is bij jou in goede handen! Jasmin, het was altijd een plezier om met je te werken. Dank voor al je hulp, je bent goud voor de groep! Jeanine, het was niet makkelijk maar je hebt je weg in het lab gevonden. Laat die 4000 epjes maar komen! De Lyme onderzoekers zijn niet compleet zonder Anneke Oei van de Medische Microbiologie. Het was fijn om te weten dat je altijd voor mij klaar stond. Je bent nauwkeurig en je hebt een bijzonder goed geheugen, waar ik dankbaar gebruik van heb kunnen maken. We hebben een goede samenwerking gehad de afgelopen jaren, waarvoor dank. Behalve kweken ben je ook een ster in het bakken, de tekentaart was een mooi cadeau!

Als ik denk aan de Lymies, dan denk ik altijd aan de ‘Tommies’, de mede-promovendi van Tom van der Poll. Waar was ik geweest zonder jullie! Ik ben dankbaar voor jullie gezelligheid en steun de afgelopen jaren. Het congres in Barcelona, de retraites in Oost-Nederland, vis-op-vrijdag en lunch-om-half-twaalf zorgde ervoor dat ik altijd met plezier op F0 en later op G2 heb gezeten. Cees en Alex, het was altijd goed om te weten dat ik bij jullie terecht kon voor moleculaire of immunologische vragen. Brandon, dankzij jou heb ik meer kunnen halen uit mijn qPCR analyses. Verder wil ik Danielle, Regina en Monique bedanken voor

Dankwoord

241

hun inspanningen om het lab met al die rondvliegende AIO’s draaiend te houden. Marieke en Joost, dank voor alle hulp bij de teken en de dierexperimenten. Ook Heleen en MoniQue bedankt voor de ondersteuning! I would also like to thank to other members of CEMM. In particular Luigi, thanks for the fun, the Italian made espressos and the delicious dinners.

Eerder zelf als student begonnen op het CEMM, heb ik als promovendus uiteindelijk mogen ervaren hoe belangrijk studenten zijn. Eduard, Willem, Floor, Jiri en Juul; veel dank! Eduard, je hebt veel werk gestopt in de AMLC patiënten en het was een mooie uitdaging om samen door de database te spitten. Willem, je kritische vragen hielde ons scherp. Floor, dankzij jou liepen de experimenten als een trein. Jiri en Juul, dank voor het verbeteren en uitbreiden van de klinische database.

Buiten het AMC wil ik nog een paar personen bedanken. Prof. Frans Jongejan van de Universiteit Utrecht, dank voor het helpen opstarten van de Ixodes ricinus tekenkolonie in het AMC. Seta, Tim en Gillian, waarmee ik in 2014 samen het TickTactics symposium heb georganiseerd, het was leuk om dit met jullie te ondernemen! My time at the Fikrig lab at Yale University has become a success thanks to a number of people. Kathy, Jingyi, Martin, Julia, Yang and Nabil, it was wonderful working with all of you.

Waar zou een promovendus zonder zijn paranimfen zijn. Duncan, als buurman en buurman op F0 hebben we elkaar goed weten te vermaken. Achter je scherpe gevoel voor humor en gevatte opmerkingen zit een goed hart. Jacob; geen moment met jou is saai. Jouw kijk op het leven en je kameraadschap heb ik altijd zeer gewaardeerd. Ik kijk er naar uit dat jullie straks naast mij staan.

Buiten het werk heb ik twee waardevolle vriendengroepen die belangrijk zijn voor mijn leven buiten het onderzoek. Arthur, Roel, Jessie, Jeanette en Astrid, onze tripjes naar Berlijn waren altijd een welkome onderbreking. Chris, James, Frank, Joost, Maarten en Paul; vrienden van het eerste uur. Jullie houden me met beide benen op de grond. Ons weekend in een kasteel in Duitsland liet maar weer eens zien hoe bijzonder onze band is. Iris en Anthe, dankzij jullie ben ik bij Joppe terecht gekomen, dank hiervoor en voor de leuke avonden samen.

Lieve ouders, jullie hebben altijd achter me gestaan. Altijd benieuwd hoe het met me ging, wat me bezig hield en heel uniek dat jullie beiden mij hebben kunnen

Dankwoord

242

opzoeken in New Haven. Ik ben blij dat ik jullie kan laten stralen. Mijn broers en zusje Boris, Janne en Hanneke; ieder erg verschillend maar een leuk stel samen. De avonturen die we de afgelopen jaren samen hebben beleefd, zoals de jaarlijkse wintersport, waren de afgelopen jaren een belangrijke uitlaatklep voor me.

Lieve Janneke, je bent het mooiste dat ik op het lab heb kunnen vinden. Je staat altijd voor mij klaar en ik ben blij dat we tussen de drukte door veel avonturen met elkaar beleven en lol hebben met elkaar. Ik ben dankbaar dat ik de laatste (en leukste) fase van mijn promotie en mijn tijd als coassistent met jou mag beleven. Ik weet zeker dat je harde werken mooie resultaten gaat opleveren. Ik hou van je.

Dankwoord

241

hun inspanningen om het lab met al die rondvliegende AIO’s draaiend te houden. Marieke en Joost, dank voor alle hulp bij de teken en de dierexperimenten. Ook Heleen en MoniQue bedankt voor de ondersteuning! I would also like to thank to other members of CEMM. In particular Luigi, thanks for the fun, the Italian made espressos and the delicious dinners.

Eerder zelf als student begonnen op het CEMM, heb ik als promovendus uiteindelijk mogen ervaren hoe belangrijk studenten zijn. Eduard, Willem, Floor, Jiri en Juul; veel dank! Eduard, je hebt veel werk gestopt in de AMLC patiënten en het was een mooie uitdaging om samen door de database te spitten. Willem, je kritische vragen hielde ons scherp. Floor, dankzij jou liepen de experimenten als een trein. Jiri en Juul, dank voor het verbeteren en uitbreiden van de klinische database.

Buiten het AMC wil ik nog een paar personen bedanken. Prof. Frans Jongejan van de Universiteit Utrecht, dank voor het helpen opstarten van de Ixodes ricinus tekenkolonie in het AMC. Seta, Tim en Gillian, waarmee ik in 2014 samen het TickTactics symposium heb georganiseerd, het was leuk om dit met jullie te ondernemen! My time at the Fikrig lab at Yale University has become a success thanks to a number of people. Kathy, Jingyi, Martin, Julia, Yang and Nabil, it was wonderful working with all of you.

Waar zou een promovendus zonder zijn paranimfen zijn. Duncan, als buurman en buurman op F0 hebben we elkaar goed weten te vermaken. Achter je scherpe gevoel voor humor en gevatte opmerkingen zit een goed hart. Jacob; geen moment met jou is saai. Jouw kijk op het leven en je kameraadschap heb ik altijd zeer gewaardeerd. Ik kijk er naar uit dat jullie straks naast mij staan.

Buiten het werk heb ik twee waardevolle vriendengroepen die belangrijk zijn voor mijn leven buiten het onderzoek. Arthur, Roel, Jessie, Jeanette en Astrid, onze tripjes naar Berlijn waren altijd een welkome onderbreking. Chris, James, Frank, Joost, Maarten en Paul; vrienden van het eerste uur. Jullie houden me met beide benen op de grond. Ons weekend in een kasteel in Duitsland liet maar weer eens zien hoe bijzonder onze band is. Iris en Anthe, dankzij jullie ben ik bij Joppe terecht gekomen, dank hiervoor en voor de leuke avonden samen.

Lieve ouders, jullie hebben altijd achter me gestaan. Altijd benieuwd hoe het met me ging, wat me bezig hield en heel uniek dat jullie beiden mij hebben kunnen

Dankwoord

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opzoeken in New Haven. Ik ben blij dat ik jullie kan laten stralen. Mijn broers en zusje Boris, Janne en Hanneke; ieder erg verschillend maar een leuk stel samen. De avonturen die we de afgelopen jaren samen hebben beleefd, zoals de jaarlijkse wintersport, waren de afgelopen jaren een belangrijke uitlaatklep voor me.

Lieve Janneke, je bent het mooiste dat ik op het lab heb kunnen vinden. Je staat altijd voor mij klaar en ik ben blij dat we tussen de drukte door veel avonturen met elkaar beleven en lol hebben met elkaar. Ik ben dankbaar dat ik de laatste (en leukste) fase van mijn promotie en mijn tijd als coassistent met jou mag beleven. Ik weet zeker dat je harde werken mooie resultaten gaat opleveren. Ik hou van je.