vce vet laboratory skills · 3 2018 vet lab skills exam section a continued turn over question 5 a...
TRANSCRIPT
VCE VET LABORATORY SKILLSWritten examination
Wednesday 21 November 2018 Reading time: 9.00 am to 9.15 am (15 minutes) Writing time: 9.15 am to 10.45 am (1 hour 30 minutes)
QUESTION AND ANSWER BOOK
Structure of bookSection Number of
questionsNumber of questions
to be answeredNumber of
marks
A 20 20 20B 17 17 80
Total 100
• Studentsarepermittedtobringintotheexaminationroom:pens,pencils,highlighters,erasers,sharpeners,rulersandonescientificcalculator.
• StudentsareNOTpermittedtobringintotheexaminationroom:blanksheetsofpaperand/orcorrectionfluid/tape.
Materials supplied• Questionandanswerbookof19pages• Answersheetformultiple-choicequestions
Instructions• Writeyourstudent numberinthespaceprovidedaboveonthispage.• Checkthatyournameandstudent numberasprintedonyouranswersheetformultiple-choice
questionsarecorrect,andsignyournameinthespaceprovidedtoverifythis.• AllwrittenresponsesmustbeinEnglish.
At the end of the examination• Placetheanswersheetformultiple-choicequestionsinsidethefrontcoverofthisbook.
Students are NOT permitted to bring mobile phones and/or any other unauthorised electronic devices into the examination room.
©VICTORIANCURRICULUMANDASSESSMENTAUTHORITY2018
SUPERVISOR TO ATTACH PROCESSING LABEL HEREVictorian Certificate of Education 2018
STUDENT NUMBER
Letter
2018VETLABSKILLSEXAM 2
SECTION A – continued
Question 1Whatinformationshouldbeincludedonthelabelforanagarstreakplatethathasbeenstreaked?A. employeename,dateofbirth,sampleidentificationB. employeename,clientname,dateofsamplereceiptC. dateofsamplereceipt,laboratoryname,roomtemperatureD. employeename,dateofsamplepreparation,sampleidentification
Question 2Whenusingacompoundmicroscopewithitshigh-powerobjectivelens,whichoneofthefollowingshouldnot beadjusted?A. coarsefocusB. diaphragmC. stageclipsD. finefocus
Question 3 Laboratorystaffhavebeeninformedbythelaboratorysupervisorthatanewmodelofsterilisingequipmentwillbeinstalledinthelaboratorythefollowingweek.Basedonthisinformation,whatactionshouldaninexperiencedtechniciantake?A. Waitforthelaboratorysupervisortotellthemwhenthetrainingforthenewequipmentmodelwilltake
place.B. Researchinformationaboutthenewequipmentmodelbeforeitarrives,tohelptraintheircolleagueson
howtouseit.C. Researchinformationaboutthenewequipmentmodel,thenaskthelaboratorysupervisorabouttraining
opportunities.D. Researchinformationaboutthenewequipmentmodelandinformthelaboratorysupervisorthattheydo
notrequireanytraining.
Question 4Thecorrectprocedureisbeingusedtoprepareprimarystandardsolutionsaccurately.Duringthisprocedure,quantitativetransferofasolidisusedinordertoA. transferanexactmassofsoluteduringpreparationofthesolution.B. operatethebalanceaccordingtothemanufacturer’sinstructions.C. findaprobableestimateofthefinalconcentrationofthesolution.D. ensurethecorrectmaterialsarechosenforpreparationofthesolution.
SECTION A – Multiple-choice questions
Instructions for Section AAnswerallquestionsinpencilontheanswersheetprovidedformultiple-choicequestions.Choosetheresponsethatiscorrectorthatbest answersthequestion.Acorrectanswerscores1;anincorrectanswerscores0.Markswillnotbedeductedforincorrectanswers.Nomarkswillbegivenifmorethanoneansweriscompletedforanyquestion.
3 2018VETLABSKILLSEXAM
SECTION A – continuedTURN OVER
Question 5A dilution of potassium chloride, KCl, is to be prepared in the laboratory. 100 mL of a 0.1 M solution of KCl is transferred to a 200 mL volumetric flask and deionised water is added to the mark. The deionised water is best identified as theA. liquid.B. solute.C. solvent.D. solution.
Question 6A laboratory technician receives a call from a client’s partner, requesting the client’s test results.What is the most appropriate action that should be taken by the laboratory technician? A. Retrieve the test results and then pass the details on to the client’s partner efficiently and politely. B. Request further identifying details about the client and their test, and inform the partner that the client’s
results are not yet ready to be released. C. Request further identifying details about the client and tell the partner that they will be informed of the
test results once the results are released. D. Explain that the test results cannot be released to anyone other than the client and request that the client
calls the laboratory for the results.
Question 7Duplicate sample test values were found to be out of the expected range.When this occurs, the sample test values should beA. retested every time. B. ignored as an odd occurrence.C. reported and noted in the workplace recording system. D. treated as a serious breakdown in quality control.
Question 8Samples from a potential new supplier of hazardous chemical reagents for a laboratoryA. should be tested and evaluated before use.B. do not need to be supplied with safety data sheets (SDS).C. should only be reordered if there are significant cost savings.D. should not be used, in order to avoid changes to important standard operating procedures (SOP).
Question 9What is the correct ergonomic posture for a laboratory technician working with a microscope in a seated position?A. straight back and neck, arms level with the bench, knees bent 45° and toes stretched to touch the floorB. straight back and neck, arms level with the bench, knees bent 45° and feet resting level on the floorC. straight back and neck, arms leaning forward onto the bench, knees bent 45° and feet resting level on
the floorD. straight back, arms leaning forward onto the bench, neck stretched towards the microscope eyepieces,
and legs relaxed and hanging from a high stool
2018VETLABSKILLSEXAM 4
SECTION A – continued
Question 10 Whenrotatingtheobjectivelensonamicroscopetoahigher-poweredlens,theA. diaphragmmustbeclosed.B. lightintensitymustbeincreased.C. lightintensitymustbedecreased.D. workingdistancemustbeincreased.
Question 11 Afoodsamplerequiringurgentbacterialcontaminationtestshasarrivedatalaboratory.ThemostappropriateactionforalaboratorytechniciantotakewouldbetoA. informthelaboratorysupervisoraboutthetestrequestandwaitforthesupervisortoassigntheworkto
alaboratorytechnician.B. testthefoodsampleimmediately,butonlyafterreferringtothetestrequestspecifications.C. testthefoodsampleimmediatelywithageneralgrowthmediatoseewhatgrows.D. checkwiththelaboratorysupervisorfirstbeforeanytestingisdone.
Question 12Thebestwaytosteriliseaninoculatingloop,whileminimisingtheformationofaerosols,istoA. soaktheloopinasterilisingsolutionfor24hours.B. placetheloopdirectlyintheblueflameofaBunsenburnerandholditthereforafullminute.C. firstheatthetipoftheloopgraduallyintheflameofaBunsenburner,beginningwiththehandleendof
thewire.D. placetheloopdirectlyintheorangeflameofaBunsenburnerandpassitbackandforthuntiltheloop
glowsred.
Question 13Alaboratorytechnicianpreparessomeagarstreakplatesfromabrothculture.Duringthenextshift,anotherlaboratorytechniciancheckstheagarstreakplatesafterincubationandobservesconfluentgrowthinallsectorsoftheplates.ThebestexplanationforthisobservationwouldbethatthepreviouslaboratorytechnicianA. didnotflametheneckofthebrothbottle.B. forgottoflametheloopbetweensectors.C. wasnotworkingneartheBunsenburnerflame.D. forgottocleanthebenchbeforestartingtheprocedureforpreparingtheagarstreakplates.
Question 14GlasswareandothersolidmaterialsthatrequireautoclavingA. donotrequirelabellingasliquidswould.B. mustonlybesterilisedwiththeautoclavesetonadrycycle.C. mustonlybesterilisedwiththeautoclavesetonawetcycle.D. canbesterilisedinamixedloadwiththeappropriateautoclavesettings.
5 2018VETLABSKILLSEXAM
SECTION A – continuedTURN OVER
Question 15Whichoneofthefollowingwouldcontributethemosttoreducingtheshelflifeofaworkingchemicalsolution?A. returninganydecantedsolutiontothebottleB. makingupasolutionimmediatelybeforeitisrequiredC. keepingthesolutioninarefrigeratorwhileitisnotbeingusedD. wrappingthesolutioninaluminiumfoilwhenitislight-sensitive
Question 16Alaboratorytechnicianwouldidentifythephysicalhazardsignforanoxidisingsubstancebywhichoneofthefollowingpictograms?
A. B. C. D.
Sources:StandardStudio/Shutterstock.com(A.,C.,D.); Migrenart/Shutterstock.com(B.)
Question 17Alaboratorytechnicianhasbeenaskedtocarryoutastarchtestonapotatosample.Whichofthefollowingpairsofstainandcolourstructureswouldshowapositiveresultwhenexaminedunderamicroscope?A. Gramstain,pinkgranulesB. iodinestain,browngranulesC. iodinestain,blue-blackgranulesD. Gramstain,purple-blackgranules
Question 18Thelaboratorysupervisorinanenvironmentaltestinglaboratoryinstructsatechniciantoasepticallytransfersomesoilsamplesformicrobialevaluation.TransferringasepticallyinthisinstancewouldbestbeachievedbyA. sterilisingeachsamplebyheatundersteampressure.B. storingculturesinaculturecollectionforfuturestudyorreference.C. applyingatreatmentthatresultsindeathorremovalofalllivingorganismsinthesamples.D. usingproceduresforhandlingcultures,mediaandequipmentthatensurenocontamination,andthat
onlythedesiredorganismsarepresent.
2018VETLABSKILLSEXAM 6
END OF SECTION A
Question 19ApathologylaboratoryreceivesanoticefromtheNationalAssociationofTestingAuthorities(NATA),Australia,aboutanauditvisit.TheinformationthattheauditorsarelikelytoconcentrateonaretheA. makesandmodelsofthelaboratoryequipment.B. laboratoryresources,proceduresanddocumentation.C. profitabilityandefficiencyofthetestingprocedures.D. speedandefficiencywithwhichthelaboratorytechnicianscompletetheirtasks.
Question 20Whatisthevolumeofa50×stocksolutionthatisrequiredtoprepare200mLofa1.0×workingsolution?A. 4.0mLB. 40.0mLC. 400mLD. 0.04L
7 2018VETLABSKILLSEXAM
SECTION B – continuedTURN OVER
Question 1 (6marks)a. Apathologylaboratorytechnicianreceivesacallfromaveterinarysurgeonrequestingan
urgentresultonasubmittedbloodsample.
Listthreepiecesofinformationthatthetechnicianwillneedtoobtainfromtheveterinarysurgeonwhilestillonthephone. 3marks
1.
2.
3.
b. Theveterinarysurgeonhasrequestedthatthesamplebetestedforbacteria.
Whatlaboratorytestwouldneedtobecarriedouttohelpidentifythepossiblestrainofbacteria? 1mark
c. Thetestcarriedoutinpart b.isexpectedtoprovideoneoftwopossibleresults.
Namethetwopossibleresultsandgiveonecharacteristicthatwouldhelptoidentifyeachresult. 2marks
Result1
Characteristic
Result2
Characteristic
SECTION B – Short-answer questions
Instructions for Section BAnswerall questionsinthespacesprovided.
2018VETLABSKILLSEXAM 8
SECTION B – continued
Question 2 (3marks)Alaboratorytechniciansuspectsthattheelectronicbalancebeingusedinthelaboratoryisinaccurate.
a. Suggestoneactionthatthetechniciancouldtaketosupportthelaboratory’s‘rightfirsttime’objective. 1mark
b. Identifytwoincidentsofwastagethattheinaccurateelectronicbalancecouldcreatewithinthecompany,ifthetechniciandidnotactpromptlytoaddressthesituation. 2marks
1.
2.
Question 3 (3marks)Fromthefollowinglist,identifythemostappropriatecleaningordisposalmethodforeachofthelaboratoryitemsgiveninthetablebelow.
biohazardwastebin sharpscontainer 1.0%hypochloritesolution
Laboratory item Appropriate cleaning or disposal method
glasscell-countingchamberorhaemocytometer
plasticflaskofcellculturemedia
usedscalpelbladesfrombiologicaldissection
9 2018VETLABSKILLSEXAM
SECTION B – continuedTURN OVER
Question 4 (5marks)Primarystandardsolutionsareusedextensivelyinanalyticalchemistrylaboratories.
a. Giveonereasonforusingprimarystandardsolutionsinlaboratorywork. 1mark
b. Whattwostepsshouldatechniciantaketomakethemselvesfamiliarwithalaboratory’sprotocolforpreparingprimarystandardsolutions? 2marks
1.
2.
c. Thequantitativetransferofsolidsisarequirementwhenpreparingprimarystandardsolutions.
Explainwhatismeantby‘quantitativetransferofsolids’andgiveonereasonforitsimportance. 2marks
2018VETLABSKILLSEXAM 10
SECTION B – continued
Question 5 (7marks)AlaboratorytechnicianhasbeenaskedtopreparebacterialsmearsandtostainthemusingtheGramstainmethodformicroscopicexamination.
a. Beforebeginningobservationoftheslides,whataretwochecksthatshouldbecarriedouttoensurethatthemicroscopeisfitforpurpose? 2marks
1.
2.
b. Identifytheobjectivelensthatshouldbeusedtoobservethebacterialsmearsonthepreparedslides. 1mark
c. Indicatewhichofthetwodiagramsofslidesbelowshowsthecorrectmicroscopeset-upformagnificationandforresolutionoftheimageonapreparedGramstainslide.Tick()theboxforyourchosenslide,AorB.
DiagramofSlideA
microscope lens
immersion oil
microscopeslide
DiagramofSlideB
microscope lens
immersion oil
microscopeslide
GivetworeasonsforyourchoiceofSlideAorSlideB. 2marks
1.
2.
d. Describetwoadvantagesofusingimmersionoilonaslide. 2marks
11 2018VETLABSKILLSEXAM
SECTION B – continuedTURN OVER
Question 6 (5marks)a. IdentifytwolaboratoryworktasksthatwouldbeincludedinaNationalAssociationofTesting
Authorities(NATA),Australia,auditonquality. 2marks
1.
2.
b. Givethreereasonswhyqualityauditsshouldbecarriedoutregularly. 3marks
1.
2.
3.
Question 7 (4marks)Thegeneraldutiesofalaboratorytechnicianincludethepreparationandpouringofnutrientagarstocksforgenerallaboratoryuse.
Whataretwoquality-controlstepsalaboratorytechniciancouldtaketoensurethatnutrientagarstocksaresterile?Giveyourreasons.
Step1
Reason
Step2
Reason
2018VETLABSKILLSEXAM 12
SECTION B – Question 9–continued
Question 8 (6marks)Workinginthemicrobiologysectionofalaboratorytoprepareagarstreakplatesisoneoftheresponsibilitiesofalaboratorytechnician.
a. Givetwowaystoidentifycontaminationonagarplatesthathavebeenstreakedforsinglecolonieswithpureculturesofbacteria. 2marks
1.
2.
b. Otherthantheuseofpersonalprotectiveequipment(PPE),givefourapproachesusedtomaintainhygienewhenworkinginthemicrobiologysectionofalaboratory. 4marks
1.
2.
3.
4.
Question 9 (5marks)Alaboratorytechnicianhasbeenaskedtoprepare250mLofaproteinsolutionof2.0%w/vbovineserumalbumin(BSA).ThetechniciannoticesthattheBSAproteinisaveryfinepowderandeasilybecomesairborne.
a. Givetwoactionsthatthetechnicianmusttakeinthissituationtoavoidpossiblesafetyhazards. 2marks
1.
2.
b. Thestandardoperatingprocedure(SOP)toprepareaBSAproteinsolutionspecifiesthatitsfinalpHmustbe7.6
IdentifyoneprecautionthatshouldbespecifiedwithintheSOPfortheprocessofdissolvingandadjustingthepHofthesolution. 1mark
13 2018VETLABSKILLSEXAM
SECTION B – continuedTURN OVER
c. BSAisabiologicalsubstanceisolatedfrombeefblood.Thisbloodmaynothavebeensourcedlocallybutfromcountriesthatmayhavehadoutbreaksofnotifiablediseases.
IdentifyoneadditionalhealthriskthatthissubstancemaypresentandsuggestwhattoavoidwhenhandlingBSA. 2marks
Healthrisk
Whattoavoid
Question 10 (3marks)IdentifyonehazardofworkingwithliquidnitrogenordryiceinalaboratoryandoneitemofPPEthatwouldneedtobeworn.Describetheriskthatmaybepresentintheenvironmentwhenworkingwiththesesubstances.
Hazard
ItemofPPE
Risk
Question 11 (2marks)Thebiosafetycabinetinalaboratoryhasbeendecontaminatedbycontractors.Thedecontaminationprocedureinvolvesusinghazardousformaldehydevapourandisolatingthelaboratorywherethecabinetislocatedforseveraldays.However,nonoticeofthisprocedurewaspostedattheentrancetothelaboratoryinthisinstance.Atechnicianhasbeenassignedtoperformaroutinecellcultureprocedureinthelaboratorythefollowingdayandnoticesthatthecabinethasbeensealedwithtape.
Suggesttwoapproachesthetechniciancouldtaketodealwiththissituation.
1.
2.
2018VETLABSKILLSEXAM 14
SECTION B – continued
Question 12 (5marks)a. Figure1belowshowsabloodsmearfromasample.
Circlethepartofthebloodsmearthatwouldidentifyseparatedbloodcellswhenobservingtheslidewithamicroscope. 1mark
slide
smear
Figure 1
b. OnFigure2below,drawalinetoidentifyandlabeleachofthefollowing:• awhitebloodcell• aredbloodcell• aplatelet 3marks
Figure 2
c. Suggestonemethodofcheckingthatablood-smear-stainedslidewillmeetthequality-controlstandardsrequiredbyalaboratory. 1mark
15 2018VETLABSKILLSEXAM
SECTION B – continuedTURN OVER
Question 13 (7marks)Atechnicianhasbeenaskedtorestockalaboratory’ssolutions.Thistaskinvolvesmakingupsomenewsolutionsofcalciumcarbonate,CaCO3,andpotassiumchloride,KCl.
a. Completethetablebelowbycalculatingthemassrequiredtomakeeachsolution.Includetheunitofmeasurementandshowyourworking. 4marks
Data
formulaweightofCaCO3 100g/mol
formulaweightofKCl 74g/mol
CalculationsformassofCaCO3
CalculationsformassofKCl
Chemical Mass Molarity Volume
CaCO3 0.4M 350mL
KCl 0.5M 100mL
b. WhatisthemostappropriatepieceofequipmentthatshouldbeusedtostoretheCaCO3andKClsolutions? 1mark
c. Thetechnicianalsomakesupa70%v/vethanolsolution.Thelabelledcontainerhasthefollowinginformation:70%v/vethanol.
Listtwootherpiecesofinformationthatwillneedtobeincludedonthislabel. 2marks
1.
2.
2018VETLABSKILLSEXAM 16
SECTION B – continued
Question 14 (6marks)Atechnicianusinga200–1000µLvariablepipettesuspectsthatthepipetteisnotalwaysdeliveringthecorrectvolume.Thetechniciandecidestoperformacalibrationprocedure.
a. Givetworeasonswhycalibrationproceduresareimportantinlaboratories. 2marks
1.
2.
b. Asthevariablepipetteismostlyusedfordelivering1000µLvolumes,thetechnicianperformsthecalibrationatthissetting,notingthattheroomtemperatureis20°Candmeasuringtheindividualmassesofdistilledwaterdeliveredbythepipettefor20deliveries.Thetechnicianthenconvertsthemassestovolumesusingtheformula
volume = massdensity
Ifthedensityofpurewaterisassumedtobe1.0000g/mLat20°C,calculatetheactualvolumedeliveredbythepipetteforadeliveredmassof0.900g. 2marks
c. Thetechniciannotesthatmostofthemassesdeliveredbythevariablepipetteareclosetothevalueof0.900gandfallwithintherangeof0.898gto0.906g.
Whatdeliveredmasswouldthetechnicianexpectifthepipettewasoperatingcorrectlyatasettingof1000µL? 2marks
17 2018VETLABSKILLSEXAM
SECTION B – continuedTURN OVER
Question 15 (3marks)Alaboratorytechnicianhasbeenaskedtocontributetoupdatingthelaboratory’sSOPforinoculatingsterilenutrientbroth(NB)fromaculturegrowingonanagarplate.Severalstepsarerequired.Thefirstfourstepsaregiveninthetablebelow.
Completethetablebyenteringtheremainingthreestepsoftheprocedure.
Step number Procedure
1 Disinfectthebenchwith70%v/vethanolorsimilar.
2 LighttheBunsenburner.
3 Gathermaterialsandensureallmaterialsarewithinreach.
4 Sterilisetheinoculatingloop.
5
6
7
2018VETLABSKILLSEXAM 18
SECTION B – continued
Question 16 (4marks)Alaboratorytechnicianispreparing250mLofa0.10Msolutionofcoppersulphate,CuSO4,using achemicalbottlewithlabelsasshownbelow.
CAUTION
Keep out of reach of children.
ACME Laboratories
Copper(II) sulphate.5H2O
Formula weight: 249.68
MIN
Assay 98.0%
Front label Side label
a. CalculatethemassofCuSO4thatwouldberequiredtoaccuratelyprepare250mLofa0.10MsolutionofCuSO4.Showyourworking. 2marks
b. ThetechnicianisrequiredtouseabottleofanhydrousCuSO4toprepareasecond250mLsolutionof0.10MCuSO4.
WouldthemassofCuSO4calculatedinpart a.stillbeaccurate?Giveyourreasoning. 2marks
19 2018VETLABSKILLSEXAM
Question 17 (6marks)Apathologylaboratoryisinvestigatingwaystoimproveitsproceduresandoutput.Oneoftheareasbeinginvestigatedistemperatureregulationinthelaboratory’sincubator.Thetemperatureoftheincubatorismonitoredandrecordedthroughoutanormalworkingday.Theresultsareshowninthegraphbelow.
8.00 am 9.00 am 10.00 am 11.00 am 12.00 noontime of day
1.00 pm 2.00 pm 3.00 pm 4.00 pm 5.00 pm
10
15
20
25temperature(°C)
30
35
40
a. Basedonthegraph,whatoperatingtemperaturehasthethermostatintheincubatorbeensettoinordertooptimiseculturegrowthatthebeginningofeachday?Expressyouranswertoonedecimalplace. 1mark
b. Thegrowthrateofculturesisnotaffectedbyatemperaturechangeof±2°C.
Usingtheinformationinthegraph,givethetwotimeperiodswhenthetemperaturedroppedbelowthisoptimumtemperaturerange. 2marks
c. Suggestonereasonforthetemperaturedropatthetwotimeperiodsgiveninpart b. 1mark
d. Usingtheinformationinthegraph,giveonereason,otherthanthetemperaturebeingoutsideofthenormalrange,foranobservedreductioninthegrowthrateoftheculturesandsuggestapossiblesolution. 2marks
END OF QUESTION AND ANSWER BOOK