VerePLEXTM Biosystem

Multiplexing Simplified

A versatile multiplexing platform for the rapid analysis of nucleic acids. Combining molecular biology, microfluidics and microelectronics to enable the integration of PCR and Microarray.

Temperature Control System (TCS)

The Temperature Control System (TCS) is a high precision thermal cycler that drives the VereChipTM. The TCS can run up to five different tests and protocols simultaneously or at different points of time without having to wait for all the modules to complete their cycles. It is modular and can be scaled to accommodate the needs of both large diagnostic settings and point-of-care applications.

Optical Reader (OR)

The Optical Reader is a compact instrument that captures and analyzes the microarray in a few seconds. The optimal optics settings are automatically selected and this makes it particularly suited for minimally-trained personnel and point-of-care applications.

VereChipTM Lab-on-Chip

VereChipTM is a silicon chip that integrates an ultra-fast miniaturized PCR reactor for amplification of nucleic acids and a customizable microarray. It allows users to apply the full benefits of molecular testing in real-world conditions, at a fraction of the time, cost and complexity needed to operate common lab equipment.

Biosystem Software

The software can provide a highly detailed microarray analysis report for expert users in a central lab or a simple diagnostic report for basic users in a point-of-care setting.

VerePLEXTM Biosystem consists of the following components:

• Temperature Control System (5 random access modules)

• Optical Reader

• Biosystem Software

• Barcode Reader

VerePLEXTM Biosystem combines molecular biology, microfluidics and microelectronics to bring the future of diagnostics and surveillance to you today. The VerePLEXTM Biosystem, along with the VereChipTM, is a breakthrough in innovation, integrating two powerful molecular biological technologies: PCR and Microarray.

VerePLEXTM Biosystem Operational Workflow

PCR mix loading01.

Running PCR02.

Washing05.

Reading06.

Hybridization mix loading

03.

Running hybridization

04.

• Processes 5 microarrays simultaneously

• Turnaround time ~ 2 hrs

VerePLEXTM Biosystem

Veredus Laboratories Pte Ltd83 Science Park Drive #04-02, The Curie, Science Park 1, Singapore 118258Phone +65 6496 8600 Fax +65 6779 2680 Email info@vereduslabs.com Website http://www.vereduslabs.com

A. A Breakthrough Innovation with the Integration of Two Powerful Molecular Biological Technologies:

• Polymerase Chain Reaction (amplification of DNA) and Microarray

• High accuracy and sensitivity

• Provides genetic information of infection

• Probe sequences can be updated to detect any current or new genetically modified biological agents

B. Miniaturization into a chip the size of a fingernail

• High level of automation and portability enable usage outside the traditional lab or hospital environment

SpecificationsTemperature Control System (TCS) • Average Ramping Rates –

Heating: typical 20°C/s; Cooling: typical 10°C/s

• Temperature Resolution: 0.1°C

• Communications: Local Area Network / TCP-IP connection

• Size and Portability: TCS measures 29cm×50cm×14cm and weighs 10kg

• Highly conductive material, integrated heaters and temperature sensors for outstanding control and speed of the PCR temperature cycles, resulting in more specific PCR products in 20-60 minutes

VereChipTM

• A 2.54cm×7.62cm slide present on VerePLEXTM Biosystem provides the necessary mechanical, thermal, electrical connections to drive the chip

• The microarray is spotted at semiconductor quality level

• Array Density—up to 500 individual probes

• Superior surface quality (microelectronic grade surface instead of micro-machined glass slide)

Principles of Technology Employed

Temperature Control SystemOptical Reader

Biosystem Software

Bio-applications VereChipTM

Combining Molecular Biology, Microfluidics and MicroelectronicsFeatures • Rapid sample to answer

• Minimal training required to operate

• Scalable with additional Temperature Control System modules

• Samples can be run independently or simultaneously

• Customizable PCR parameters

• 40% faster than conventional thermal cyclers

• Higher specificity due to outstanding annealing temperature precision

• Programmable temperature ramp rate

• Advanced microarray design

• Biosystem software performs automatic microarray grid alignment

• Microarray has optical interference layers resulting in greater sensitivity

• Optical Reader contains high powered LED light source