verification of lasting antiviral effect against covid-19 virus … · 2 days ago · the...

Verification of Lasting Antiviral Effect

against COVID-19 Virus (SARS-CoV-2)

by Takex Clean Extra

Takex Co., Ltd.

Takexco Malaysia Sdn. Bhd.http://takex-co.com

1

© 2021 Takex Co., Ltd. All Rights Reserved.

Company Name Takex Co., Ltd.

Established 8 May, 2017

Address 1-23-5, Esaka cho, Suita shi, Osaka Japan

President and

CEOKumi Okada

Capital JPY100,000,000

Business 1. Production and sales of sanitizer using bamboo as raw material

2. Production and sales of disinfectant agents using bamboo as raw material

3. Production and sales of food freshness-keeping agents using bamboo as raw material

4. Production and sales of food shelf-life extender using bamboo as raw material

5. Production and sales of deodorizing agents using bamboo as raw material

6. Sales of building materials using bamboo as raw material

7. Import, export and sales of halal food8. All operations incidental to each of the above

Website https://takex-co.com/

Company Name TAKEXCO Malaysia SDN. BHD. (1246074-T)*A subsidiary of Takex Corporation.

Established 8 September, 2017

Address Unit 08, Level 6, Menara MBMR, No.1, Jalan Syed

Putra, 58000 Kuala Lumpur, Malaysia.

Representative COO Kosei Sugawara

Capital MYR1,010,000.-

2

Company Profile


Takex places the product safety standards on “mother’s viewpoint” that is

the most demanding standard.We will support the mother’s wishes for the health of her children and family by producing safe and reliable products.

https://takex-co.com/

We have been dedicated to the research and development ofbamboo for more than 30 years since we had developed

antibacterial agents extracted from bamboo in 1987

Developed the antibacterial

agents from bamboo extracts

by the joint research with

Public Health, Kitasato

University Schoolof Medicine. In the same year, announced the research results in “The Society for Antibacterial and Antifungal Agents, Japan”.

The antimicrobial effect of the bamboo extract was confirmed against 14 bacteria.(joint research with Medicine Department of Bacteriology, Osaka City University)

Announced “Confirmation of bamboo extract of inactivation effect against virus“(joint research with Osaka Institute of Public Health)

Announced “Bamboo extract has 99.99999% effectiveness in reducing the viral infectivity rate of avian influenza virus” (joint research with the Avian Zoonoses Research Center, Faculty of Agriculture, Tottori University)

Announced "The effectiveness and the mechanism of action of the bamboo extract against feline calicivirus (a substitute for norovirus).“(joint research with Graduate School of Microbiology and Veterinary Science, Osaka Prefecture University)

Confirmed the effect of a bamboo extract agent against coxsackievirus (hand, footand mouth disease)(joint research with KitasatoResearch Center for Environmental Science)

Disinfectant was certified

as halal by JAKIM

Confirmed "The effects of incinerated ash mixture of bamboo and bergagainst avian influenza”(joint research with Tottori University).

Started operation of the factory.TAKEX CLEAN and TAKEX CLEAN EXTRA were certified as halal by JAKIM

Established a factory in Malaysia

Established Takexco Malaysia as a subsidiary of

Takex Japan.

Business History

1987

1996

2006

2009 2013

2016

2018

2018

2017

2017

3

2009


4

Product Features

Wide range of antibacterial effect

Effective even in wet conditions

Naturally derived and

human-friendly

Excellent

deodorizing effect

Combining the power of bamboo extract and ethanol,

Takex products cover the weaknesses of existing ethanol sanitizers.

Widely effective against viruses and bacteria that cannot be expected to be effective with existing ethanol sanitizers.

Effective even in wet conditions that cannot be expected to be effective with existing ethanol sanitizers.

Excellent durability

and sustainability

Confirmed that the antibacterial effect that could not be expected after volatilization sustains even on the 7th day. Prevents secondary infections.

All the ingredients used, including bamboo extract, are certified as raw materials for food additives in Japan and can be sprayed directly onto the ingredients.

The effect of bamboo extract decomposes and deodorizes various odors from the source.

Spray directly on foodstuffs, places and

things that you frequently touch.

doorknobs tables and chairs kitchen prams toys kitchen utensils shoes and boots beds and pillows

Recommended use


hands

5

What Is Bamboo Extract?

Bamboo extract is obtained by extracting bamboo skin powder of

bamboos grown in China and Japan with ethanol. Bamboo

extract is highly safe as it is officially categorized as a food

additive by Ministry of Health, Labour and Welfare in Japan.

Bamboo quinine powdercollected separatelyfrom bamboo skin.

Bamboo Extract

(List of existing Food Additives 323)

[Specific skill of Takex group]

Bamboo

The safety of bamboo extract

①Repeated-dose toxicity testA test to see the influence of continuous administration.

With the test using rats for 90 days, no toxicological effects were observed.

②Mutagenicity testA test to see if it would have chromosomal abnormality. No mutagenicity was observed.

③Acute toxicity testAs for the acute toxicity test conducted using rats, no acute toxicity was observed

at 5,000 mg / kg administration.

Conducted below safety verification tests.

①②Research on safety evaluation of existing additives②Conducted by Shinshu University (Department of Agriculture)③Conducted by Kitasato University (Department of Public Health)

[Test condition]

• heating temperature: 120℃• tryptone-glucose-yeast extract agar

(37℃ 24 hours)• paper-disk method

Effective for wide range of bacteria

Antioxidative effect Activation at hotenvironment condition

*Effective widely such as pathogenic bacteria, bacteria capable of causing food poisoning, putrefactive bacteria and virus.

Test report from Osaka City University

Graduate of Faculty of Human Life Science

Reported at Japan Society for Bioscience,

Biotechnology, and Agrochemistry in 1998

and 1999.

Efficacy of bamboo extract


Escherichia coli O-157 Legionella pneumophila

Salmonella MRSAMethicillin-resistant

Staphylococcus Aureus

Antioxidative effect of bamboolignan extract

Absorb

ance(5

00ppm

)

Control

Bamboolignan

days

1.Antibacterial effect

Inhibit bacterial growth2. Deodorant performance

resolve the origin of the odor

3. Antioxidative effect

Prevent oxidation

4. Others

[active ingredient]

2,6 Dimethoxyphenol,

-1,4 benzoquinone

[active ingredient]

•furofuran lignan

•polyphenol

[active ingredient]

•Phenol

•flavonoid

[active ingredient]

•Chlorophyll

•beta carotene

•xanthophyll

•violaxanthin

Effect of Bamboo Extract against Virus

6

Bamboo extract prevents infection by

inhibiting the cognitive action of spikes in

the virus.

(The virus infects by recognizing host

cells with spikes.)

spike

shell

Bamboo Extract

Host cell Host cell

infected Not infected Not acting on host cells.

Not acting on genes.

Gene（RNA）

Acting on spikes.

Gene（RNA）

Mechanism of action on virus

Inactivate 99.999684% of virus

Inactivate 99.999999% of virus

Inactivation test against Feline calicivirus, substitution of norovirus

Inactivation test against bird flu virus

[Test center] Graduate School of Microbiology and

Veterinary Science, Osaka Prefecture

University

[Test method] Let virus liquid, citrate buffer and bamboo

extract in vitro, and TCID50 (50% tissue

culture infective dose) was calculated by

the Reed-Muench method. Let virus liquid

and citrate buffer as a control test.

[Test virus] Feline calicivirus (F9 strain)

[Test sample] Add 5% of dry solid bamboo extract

[Test result] Inactivate 99.99% and more of viruses by

acting for 30 minutes.

[Test center] Avian Zoonoses Research Center, Faculty

of Agriculture, Tottori University

[Test method] Let virus liquid and bamboo extract act in

vitro, and EID50 (50% Embryo Infectious

dose) was calculated by the Reed-Muench

method. Let virus liquid and sterile water

as a control test

[Test virus] Bird flu virus (H5N3)

[Test sample] 5% of dry solid bamboo extract

[Test result] Inactivate 99.99% and more of viruses by

acting for 2 hours.


1. RT-PCR confirms that bamboo extract does not act on feline calicivirus gene

2. Confirm that bamboo extract does not act on host cell

Based on the above results and the structure of feline calicivirus, the site where bamboo extract works is

considered to be a virus spike. The existence of a site that recognizes the host cell has been reported in the

spike, and it is considered that the bamboo extract inhibits the recognition function thereof, thereby

suppressing the growth of the virus.

Takex Co., Ltd. confirmed lasting antiviral effect of Takex Clean Extra with the test conducted by Japan Textile Products Quality and Technology Center.*Destroy (eliminate the infectivity of) a microbe (a virus in this case)

Lasting Antiviral Effect* against COVID-19 Virus (SARS-CoV-2) by Takex Clean Extra

Sample Test virus Duration

Antiviral

activity

value

Virus

inactivation rate

(%)

Takex Clean ExtraCOVID-19 virus

(SARS-CoV-2)15 days ≧ 4.3 99.99% and more

100％

0

50

100

Control Takex Clean Extra

Inactivate 99.99% and more of virus

Less than 0.01%

Remove 99.99%

and more of virus

Takex Clean Extra

Inactivation effect against COVID-19 virus

[Test center] Japan Textile Products Quality and Technology Center

[Test method] Test standard: IS21702

Apply the test sample on 50㎜ x 50㎜ plates. After covering with a PE film, store it in a Petri dish. Leave it at 25℃ for 24 hours.Wash out the virus and determine the viral infectivity by plaque assay.

[Test virus] COVID-19 virus (SARS-CoV-2)

[Test liquid] Takex Clean Extra

[Test sample] Plates that have been applied with test liquid and dried (50㎜ｘ50㎜)Unprocessed plates were used as the control.

[Duration] 15 days

(Applied with virus suspension 15 days after preparing plates.)

[Test result] Confirmed that 99.99% and more of the virus became inactive by

letting the virus suspension for a certain period of time.

Test Details

We are also conducting a persistent antivirus verification test of

Takex Clean Extra against COVID-19 virus in Jiangsu Provincial

Centers for Disease Control and Prevention (China).* Jiangsu Provincial Centers for Disease Control and Prevention (briefly

as Jiangsu CDC, 江苏省疾病预防控制中心 in Chinese) is Jiangsu Institute For Public Health in Jiangsu, China. It belongs to The Chinese

Center for Disease Control and Prevention which is the center at the

government and national level. Its main duties are prevention and control

of infections diseases and emergency response.

http://www.jshealth.com/

Virus s

urv

ival ra

te (

%)

7© 2021 Takex Co., Ltd. All Rights Reserved.

http://www.jshealth.com/http://www.jshealth.com/

Brief Explanation of Lasting Antiviral Verification Test

Spray

Takex Clean

Extra and let it

to dry

How to

act on virus

Processing

methodPreparing test plate

UntreatedHow to

act on virus

Residual

active ingredients

for 10 days or 15 days

Start After acting on virus

No antiviral effects

Shows antiviral effects

Bamboo Extract

Bamboo extract remains on plate after ethanol

has evaporated.

8

Test Details


Sample Test virus Duration

Antiviral

activity

value

Virus

inactivation rate

(%)

Takex Clean Extra

Feline calicivirus (F-9)

10 days

4.4 99.99% and more

Influenza A virus (H3N2) ≧4.0 99.99% and more

100％

0

50

100


Inactivate 99.99% and more virus

Less than 0.01%

Remove 99.99％

and more virus

Takex Clean Extra

[Test center] Japan Textile Products Quality and Technology Center

[Test method] Test standard: IS21702

Apply the test sample on 50㎜ x 50㎜ plates. After covering with a PE film, store it in a Petri dish. Leave it at 25℃ for 24 hours.Wash out the virus and determine the viral infectivity by plaque assay.

[Test virus] Feline calicivirus (F-9)

Influenza A virus (H3N2)

[Test liquid] Takex Clean Extra

[Test sample] Plates that have been applied with test liquid and dried (50㎜ｘ50㎜)Unprocessed plates were used as the control.

[Duration] 10 days

(Applied with virus suspension 15 days after preparing plates.)

[Test result] Confirmed that 99.99% and more of the virus became inactive by

letting the virus suspension for a certain period of time.

Verification Test Results against other viruses

Lasting Antiviral Effect* against Viruses

*Feline calicivirus substitute of Norovirus

Feline calicivirus (F-9) / Influenza A virus (H3N2)


Takex Co., Ltd. confirmed lasting antiviral effect of Takex Clean Extra with the test conducted by Japan Textile Products Quality and Technology Center.*Destroy (eliminate the infectivity of) a microbe (a virus in this case)

Virus s

urv

ival ra

te (

%)

10

Sample Test bacteria Duration

Log-mean of

viable bacterial

count

Antibacterial

activity

value [R]

Antibacterial

activity

value (%)

Takex Clean Extra

Escherichia coli

10 days

＜－0.20 ≧5.499.999% and

more

Staphylococcus

aureus＜－0.20 ≧2.2 99% and more

100％

0

50

100


Remove 99.999% and more of virus

Less than 0.001%

Remove

99.999％and more

virus

Removal effect on Escherichia coli

[Test center]

Japan Textile Products Quality and Technology Center

It has been confirmed to reduce viable bacteria count by letting act on bacteria

10 days after applying Takex Clean Extra on plates. It can be said that Takex

Clean Extra has a lasting antibacterial effect (for 10 days).

Verification Test Results against Bacteria


Lasting Antibacterial Effect against Escherichia coli and Staphylococcus aureus

Surv

ival ra

te o

f via

ble

bacte

ria c

ount (%

)

11

External Test Results conducted by Research Industries

Japanese product effectiveness against wide range of viruses and bacteria


No. MicrobesRemarks:

test summaryTest laboratory

when started 30 sec. later 5 min. later 30 min. later log TCID50/ml

Takex Clean Biz 6.7 ND ND ND Not detected (ND): ＜2.5

purified water 6.7 *** *** 6.8 Not administered (NA): ***

when started 15 sec. later 30 sec. later 1 min. later log TCID50/ml

Takex Clean Biz (50 %) 6.5 ND ND ND Not detected (ND): ＜1.5

purified water 6.5 *** *** 6.5 Not administered (NA): ***

when started 1 min. later 5 min. later 15 min. later

Takex Clean Biz (x3 dilution) 4.9×10⁶ ND ND ND Not detected (ND): ＜10

phosphate-buffered dilution 4.9×10⁶ *** *** 4.9×10⁶ Not administered (NA): ***


Takex Clean Biz (x3 dilution) 3.9×10⁶ ND ND ND Not detected (ND): ＜10

phosphate-buffered dilution 3.9×10⁶ *** *** 3.0×10⁶ Not administered (NA): ***


TAKEX CLEAN Biz (x3 dilution) 7.3×105 ND ND ND Not detected (ND): ＜10

phosphate-buffered dilution 7.3×105 *** *** 5.4×10

5 Not administered (NA): ***






TAKEX CLEAN Biz (undiluted) 9.0×104 50 ND ND Not detected (ND): ＜10

phosphate-buffered dilution 1.0×10⁵ *** *** 9.0×104 Not administered (NA): ***


TAKEX CLEAN Biz (x3 dilution) 1.0×10⁶ ND ND ND Not detected (ND): ＜10

phosphorolysis buffered dilution 3%NaCl 1.0×11⁶ *** *** 1.0×11⁶ Not administered (NA): ***

number of test 22 days later 34 days later

TAKEX CLEAN Biz (dry & undiluted) first time 10 ND Not detected (ND):＜10

laboratory dish (nothing sprayed) first time 8.4×10⁵ 8.4×10⁵

TAKEX CLEAN Biz (dry & undiluted) second time ND ND Not detected (ND):＜10

laboratory dish (nothing sprayed) second time 6.5×105

6.5×105



purified water 2.0×105 *** *** 3.0×10



TAKEX CLEAN Biz (x3 dilution) 9.7×10⁶ ND ND ND Not detected (ND): ＜10

phosphate buffered saline 9.7×10⁶ *** *** 1.1×107 Not administered (NA): ***


TAKEX CLEAN Biz (undiluted) 3.0×104 ND ND ND Not detected (ND): ＜10



Japan Food Research Laboraroties

Osaka Food Hygiene Association

food analysis center

Japan Food Research Laboraroties

Osaka Food Hygiene Association

food analysis center

⑥

Methicillin-resistant

Staphylococcus aureus

（MRSA）⑨

⑫ Trichophyton

Vibrio parahaemolyticus

④ Escherichia coli O157-H7

⑩ Legionella pneumophila

Test result

➀ Feline calicivirus

③

Aspergillus niger⑦

② Influenza virus

Campylobacter jejuni

⑤

⑪ Pseudomonas aeruginosa

⑧

Staphylococcus aureus

Salmonella

* In addition to above below results, we also conducted third-party institutional tests and in-house tests.

12

At nursing home

To kill bacteria ondoorknobs and handrails

To kill bacteria and odorat toilet

To kill bacteria and odorof wheelchairs

To kill bacteria and odorof garbage

To kill bacteria at sinksTo kill bacteria and odorof beds

At restaurants

To kill bacteria of kitchen utensils

To kill bacteria ofto-go trays

To kill bacteria ontables and chairs

Recommended Use

To kill bacteria and odorof garbage

To kill bacteria at sinks

To kill bacteria and odorat toilet


Personal use

To kill bacteria on doorknobs

To kill bacteria on tables and chairs

To kill bacteria at kitchen

To kill bacteria of toys

To kill bacteria of kitchen utensils

To kill bacteria of washroom

To kill bacteria of prams

To kill bacteria on hands

13

Business Summary

Business Feature: We develop, produce and distribute sanitizers and food

shelf-life extenders containing active ingredients extracted

from bamboo.

Products

Target: We are expanding our business not only in Japan but also overseas

and Halal market.

[Antibacterial and Disinfectant]

To prevent infection and mass food poisoning

[Freshness Retention]

To extend the best before date of strawberries

[Food Shelf-LifeExtender]

To extend the best before date of processed food

[Sterilization Detergent]

To preserve the freshnessof fresh food


Products distributed in Malaysia and overseas

Berry Very

Protects strawberries from mold and damage and supports confectionery making• Trehalose × Bamboo Extract

TAKEX CLEAN EXTRA/ MAZTEX CLEAN EXTRA/ TAKETSUYU

• Food-grade halal ethanol sanitizer that supports

food safety and reliability• Certified as halal for sanitizer using food-grade

ethanol by JAKIM (Jabatan Kemajuan Islam Malaysia)

• Produced in a Malaysia factory• 18L / 5L / 1L(hand pump) / 500ml(spray,

photos on right) / 100ml(spray) sizes are available

14

Product Lineup

Distributed in Thailand[TAKETSUYU]

Distributed in Indonesia[MAZTEX CLEAN EXTRA]

Distributed in Malaysia and Singapore

[TAKEX CLEAN EXTRA]

Takex Fresh

Available for vegetable and marine product processing• Shelf-life extender containing ethanol• Prevents discoloration of vegetables after processing• Inhibits the growth of bacteria


Takex Keep

Available for cooked rice, side dishes, fish paste products,noodles, western and Japanese confectioneries

• Trehalose × Bamboo Extract• Improves storage life, texture and water retention. Ethanol free• Improves shelf life by inhibiting yeast and yeast or heat resistant

bacterial spores.

Thank you.


verification of lasting antiviral effect against covid-19 virus … · 2 days ago · the...

Documents