KLEU College of Pharmacy, BelagaviB.Pharm V-Semester (RS2) (2015-16)

Pharmaceutical Biotechnology- Multiple Choice Questions

SI. No.

Content Answer

Unit- I/ Applied Microbial Technology 1. Unicellular organism:

A)Yeast C) Protozoa

B) BacteriaD) Virus

2 Viruses are:A) Parasite C) Obligate intracellular parasite

B) SaprophiteD) Obligate intracellular saprophite

3 Alcohol is produced form:A) Yeast B) Mold

C) BacteriaD) Actinomycetes

4 The Gram stain and acid-fast stain are based on chemical properties of the:A) Plasma membrane C) Cell walls

B) Outer membrane D) Periplasmic space

5 Primary screening of organic acid and organic amine producing organisms identified by:A) pH indicating dyeC) Dilution method

B) Crowded plate techniqueD) Gradient plate technique

6 Microorganism isolation is:A) Purification of culture C) Separation of a single colony

B) Introduction of inoculums D) To grow microorganisms on surface

7 Streptomycin is produced from:A)Streptomyces griseus C) Streptomyces aureofaciens

B) Micromonospora purpureaD) Streptomyces erythreus

8 Pencillin is produced from the organism:A) Penicillium chrysogenum C) Penicillium griseofulvin B) Cephalosporium acrimonium

D) Streptomyces venezuelae9 Streptomycin is an:

A) Aminoglycoside C) Quinolones

B) Beta lactam antibioticsD) Macrolide

10 Actinomycetes are:A)Eukaryotes B) C) Quinolones

C) Beta lactam antibioticsD) Macrolide

11 The best medium for the production of Penicillin isA) Nutrient agar B) Corn steep liquorC) Sulfite waste liquor D) Whey

12 Penicillin is recovered from the fermentation broth by:A) Precipitation C) Chromatography

B) Solvent extractionD) Adsorption on charcoal

13 Riboflavin is produced from:A) Clostridium butyricum C) Saccharomyces cerevisae

B) Acetobacter suboxydans D) Torula lactosa

14 Penicillin is discovered by:A)Robert koch C) Louis pastuer

B) Edward jennerD) Alexander fleming’s

15 Vitamin c is produced by fermentation using the microorganism:A)Acetobacter suboxydansC) Saccharomyces pombe

B) Aerobacter aerogenes D) Saccharomyces cerevisiae

16 Penicillin is sterilized by:A) Moist heat C) Autoclave

B) Dry heat D) Chemical agent

17 Streptomycin is produced from:A)Molds C) Virus

B) Protozoa D) Actinomycetes

18 Industrial production of streptomycin involves:A)Anaerobic submerged technique C) Anaerobic Surface culture

B) Aerobic submerged technique D) Aerobic surface culture

19 Highest yield of streptomycin requires:A) Nitrogen source C) Carbon source B)Meat extract

D) Minerals 20 Proteolytic activity of Streptomyces griseous releases :

A)Ammonia C) Calcium

B) Carbon D) Glucose

21 Streptomycin is available as:

A) Sulphate form B) Hydrochloride form

C) Sodium salt form D) Base form

22 Industrial production of streptomycin involves:

A)Anaerobic submerged technique B) Aerobic submerged technique

C) Anaerobic Surface culture D) Aerobic surface culture

23 Enzymes responsible for alcoholic fermentation:  A) Ketolase B) Zymase

C) Peroxidase D) Oxidase

24 Which type of spores are produced sexually?:

A) Conidiospores B) Sporangiospores

C) Ascospores D) Chalmidospores

25 In the citric acid production, the pH to be maintained in the fermentor is:A) 3.5 B)5.0 to 6.0C) 8.0 to 9.0 D) 1.0 to 6.0

26 Cobalt is added as precursor in the fermentation of : 

A) Vitamin B 12 B) Vitamin C

C) Vitamin B 6 D) Vitamin D

27 The required temperature for the production of citric acid is:

A) 10oC – 80oC B) 30oC – 50oC

C) 20oC – 50oC D) 25oC – 30oC

28 The by-product during streptomycin production is:A) Vitamin A B)Vitamin B2

C) Proline D) Vitamin B 12

29 β-lactum ring is present in:

A) Erythromycin Disk plate method B) Streptomycin

C) Penicillin D) Tetracyclines30 Aspergillus niger is used generally for the production of:

A) Ethanol B) Penicillin

C) Citric acid D) Lactic acid31 The raw material for citric acid production is:

A) Corn B) MolassesC) Starch D) Whey

32 Citric acid is produced in aerobic conditions by the fungi:A) Aspergillus niger B) SaccharomycesC) Agaricus bisporus D) Chrysogenum notatum

33 Phages are:A) Bactria B) Fungi

C) Protozoa D) Virus34 Citric acid is used as:

A) Acidulant B) As an antioxidant C) As preservative D) Pickling agent

35 In the production of ethanol industrially the yeast used is:A) K.pneumoniae B) Kluyreromyces fragilis C) S. cerevisiae D) Aspergillus niger

36 Clostridium acetobutylicum is used for the production of:A) Acetone – Butanol B) Ethanol

C) Vitamin-B12 D) citric acid

37 Citric acid is used as:A) Flavoring agent in food B) As an antioxidant C) As preservative D) Pickling agent

38 The most common substrate used in distilleries for the production of ethanol is:

A) Molasses B) Corn meal

C) Soya meal D) Ground gram

39 Industrial Production of Vitamin-B12 is from:A) Propionibacterium sps. B) Pseudomonas sps.

C) Pseudomonas sps.+ Propionibacterium sps. D) Candida sps

40 The penicillin produced in large scale submerged fermentation is:

A) Penicillin A B) Penicillin G

C) Penicillin D D) PAP

41 The pH to be maintained for the production of penicillin is:

A) 7.5 B) 6.5

C) 8.0 D) 5.0

Unit _II / Enzyme Technology42 Support material used for immobilization of enzyme by adsorption method:

A) Cellulose B) Gelatin

C) Alginate D) Glutaraldehyde

43 Advantage of immobilization of enzyme:

A) High rate of reaction B) less stable

C) Continuous use D) Low rate of reaction

44 Medium containing all nutrient s in excess but controlling one nutrient is referred as :

A) Turbidostat B) Chemostat

C) Continuous culture D) batch culture

45 Methods used to get immobilized enzymes:

A) Adsorption B) Absorption

C) Chemical reaction D) Ion exchange

Unit –III/Bioprocess Technology

46 First Pasteur conducted fermentation experiments in: 

A) Milk C) Fruit juices

C) Fruit juices D) Milk and fruit juices

47 Fixed bed fermentor is also known as:

A) Immobilized cell fermentor B) Fed batch fermentor

C) Tower fermentor D) Cyclone column fermentor

48 Tower fermentors are used for the production of:

A) Enzymes B) Beer

C) Antibiotic D) Vitamins

49 Downstream processing involves:

A) Purification B) extraction

C) Production D) extraction and purification

50 Upstream processing involves:

A) Development & production B) medium selection

C) Production D) recovery of product

51 The capacity of laboratory fermentor is:

A) 12-15 lt B) 2 lt

C) 500 lt D) 10 lt

52 The overheating of fermentor during fermentation is controlled by

A) Cooling agent B) Steam

C) Cool air D) Air bubbling

53 In fermentor the top portion left without broth is called:

A) Shaft B )Impeller

C) Sparger D) Head space

54 The purification and recovery of product after fermentation is called:

A) Upstream processing B) Downstream processing

C) Surface fermentation D) Submerged fermentation

55 Production of desirable product in the fermentor

is called:

A) Upstream processing B) Downstream processing

C) Surface fermentation D) Submerged fermentation

56 Batch fermentation is also called:

A) Open system B) Fed batch

C) Closed system D) Continuous fed batch

57 Antifoam agent is

A) Silicon compound B) Corn oil

B) Soyabean oil C) All the above

Unit- IV/Genetic Engineering

58 Restriction endonuclease is employed for cutting:

A) A single stranded DNA B) Double stranded DNA

C) RNA fragment Anaerobic Surface culture D) mRNA culture

59 Restriction endonucleases are:

A) Used in genetic engineering for uniting two DNA molecules

B) Used for in vitro DNA synthesis

C) Present in mammalian cells for degeneration of DNA of dead cells

D) Synthesized by bacteria for their defense

60 Structure of DNA was given:

A) Kornberg B) Nirenberg

C) Watson and Crick D) Holley and Nirenberg

61 The basic unit of a nucleic acid is

A) Pentose sugar B) Nucleoid

C) Nucleoside D) Nucleotide

62 The enzyme taking part in joining two ends of DNA is:

A) Ligase B) Polymerase

C) Gyrase D) Helicase

63 In callus culture, roots can be induced by the supply of:

A) Auxin and no cytokinin B) Higher concentration of auxin and lower concentration of

cytokinin

C) Higher concentration of cytokinin and lower concentration of auxin

D) Both auxin and cytokinin in equal proportions

64 In double helix of DNA, the two DNA strands are:

A) Coiled around a common axis B) Coiled around each other

C) Coiled differently D) Coiled over protein sheath

65 It is now possible to breed plants and animals with desired characters through:

A) Genetic engineering B) Chromosome engineering

C) Ikebana technique D) Bonsai technique

66 Plasmids are vectors for gene cloning because they

A) Replicate freely outside bacterial cells B) Self replicate in bacterial cells

C) Can be multiplied in cultures D) Can be multiplied in laboratories using enzymes

67 DNA is composed of repeating units:

A) Ribonucleosides B) Deoxyribonucleosides

C) Ribonucleotides D)Deoxyribonucleotides

68 The gene transfer occurs by:

A) Transformation B) Transduction

C) Conjugation D) Cell fusion

69 Gene is segment of:

A) RNA B) DNA

C) RNA or DNA D) Both DNA and RNA

70 Bacterial plasmid contains:

A) RNA B) RNA + protein

C) DNA D) Photosynthetic structures

71 Choose the correct statement:

A) DNA is hereditary material B) RNA is hereditary material

C) DNA is hereditary material but where it is absent RNA can function as hereditary material

D) Both DNA and RNA are hereditary materials

72 In recombinant DNA technology a plasmid vector is cleaved by:

A) Modified DNA ligase B) A heated alkaline solution

C) The same enzyme that cleave the donor DNA

D) The different enzyme other than that cleave the donor DNA

73 The most common plasmid vector used in genetic engineering is:

A) PBR 328 B) PBR 322

C) PBR 325 D) PBR 330

74 Eco RI is an

A) Ligase B) Polymerase

C) Restriction enzyme D) Gyrase

75 Extranuclear genetic material is found in:

A) Plastid and nucleus B) Mitochondria and plastids

C) Nucleus and cytoplasm D) Mitochondria and nucleus

76 The molecular formulae of deoxyribose sugar and ribose sugar respectively are:

A) C5 H10 O4 and C5H10O6 B) C5 H10 O4 and C5H10O5

C) C5 H10 O5 and C5H10O4 D) C5 H10 O5 and C6H10O4

77 DNA differs from RNA in:

A) Presence of deoxyribose sugar B) Presence of thymine base

C) Property of replication D) All the above

78 RNA contains:

A) Hexose C) Fructose

B) Ribose D) Glucose

79 Similarity between DNA and RNA is that both have:

A) Similar sugars C) Similar pyrimidines

B) Similar mode of replication D) Polymers of nucleotides

80 A small, circular DNA molecule used as a vector to transmit foreign DNA is :

A) Plasmid B) Liposome

C) Ring chromosome D) Primer

81 The first drug produced using recombinant DNA technology was:

A) Streptokinase B) Insulin

C) Erythropietin D) Human growth hormone

82 The polymerase chain reaction is used to: 

A) Create millions of copies of an interesting piece of DNA

B) Make more copies of DNA polymerase

C) Speed the rate of DNA replication in cells D) Copy protein into RNA

83 Genetic engineering manipulates gene products at the level of: 

A) The protein B) Amino acids

C) DNA D) RNA

84 A molecule that consists of a piece of DNA from one organism combined with the DNA from a member of another species is called: 

A) Transgenic DNA B) Bioengineered DNA

C) Recombinant DNA D) Restricted DNA

85 The functions of plasmid are: 

A) DNA replication B) Protein synthesis

B) Protein synthesis D) Genetic engineering

86 Annealing in genetic engineering is:

A) Joining of DNA B) Cutting of DNA

C) Joining of RNA D) Slicing of RNA

87 Which of the following bacteria is not a source of Restriction endonuclease

A) Haemophilus influenzae B) Escherichia coli

C) Agarobacterium tumefaciens D) Bacillus amyloli

88 The first step in polymerase chain reaction (PCR) is

A) Denaturation B) Primer extension

D)Annealing E) Cooling

89 What is another name for a restriction enzyme?:

A) Vector B) Plasmid

C) Restriction endonucleases D) Restriction sequence

Unit-V/Biologicals and Biopharmaceuticals90 In tissue / bacterial culture nutrients are sterilized through:

A) Water bath at 200° C B) Dry air oven at 200° C

C) Dehumidifier D) Autoclave

91 In tissue culture, callus can be induced to form shoot or root by altering the ratio of:

A) Auxin to cytokinin B) Cytokinin to ethylene

C) Auxin to gibberellin D) Gibberellin to cytokinin

92 Genetically engineered bacteria are being used in commercial production of:

A) Melatonin B) Testosterone

C) Human insulin D) Thyroxin

93 A totipotent cell means:

A) An undifferentiated cell capable of developing into a system or entire plant

B) An undifferentiated cell capable of developing into an organ Testosterone

C) An undifferentiated cell capable of developing into complete embryo

D) Cell which lacks the capability differentiate into an organ or system

94 Callus is

A) Tissue that forms embryo B) An insoluble carbohydrate

C) Tissue that grows to form embryoid D) Unorganized actively dividing mass of cells

95 Development of shoot and root in tissue culture is determined by:

A) Cytokinin and auxin B) Enzymes

C) Temperature D) Plant nutrients

96 Raising of plants from a small tissue in culture is known as:

A) Macro production C) Tissue culture

B) Micro propagation D) Mass production

97 Which of the following is not tool of genetic engineering?:

A) Vectors C) Foreign DNA

B) Enzymes D) GMO

98 Industrially important Antibiotic producing organisms shall be isolated by:

A) Disk plate method C) Direct plate method

B) Serial dilution method D) Crowded plate method

99 Vaccines are prepared from killed microbes, they are

A) Inactivated (killed) vaccine C) Autogenous vaccine

B) Attenuated vaccines D) Toxoids

100 Vaccines prepared from toxins and chemicals are:

A) Cellular vaccines C) Attenuated vaccines

B) Sub-cellular vaccines D) Heterologous vaccines

101 Hybridoma technique was first discovered by:

A) Kohler and Milstein C) Herelle

B) Robert Koch D) Land Steiner

102 The capacity of a given strain of microbial species to produce disease is known as: 

A) Pathogen C) Infection

B) Virulence D) Disease

103 Term vaccine was coined by: 

A) Robert Koch B) Needham

B) Pasteur D) Land Steiner

104 The bacterial cells are at their metabolic peak during: 

A) Lag phase C) Stationary

B) Log D) Decline

105 Toxins or enzymes which are not produced by streptococcus pyrogens: 

A) Hyaluronidase C) Hemolysin

B) Phosphate D) Streptokinase

106 Attenuation means: 

A) Killing of the bacteria (microorganism) C) Inactivation of bacteria

B) More activating the bacteria D) killing and inactivation of bacteria

107 The method used to maintain a continuous culture:

A) Chemostat C) Thermostat

B) Autostat D) Chemostat and thermostat

108 The organisms which can grow both in presence and absence of oxygen:

A) Aerobes C) Faculative anaerobes

B) Anaerobes D) Strict aerobes

109 In which medium the hydridoma cells grow selectively?:

A) Polyethylene glycol C) Hypoxanthine aminopterin thyminine

B) phosphoribosyl transferase D) Molasses

110 The enzymes which are commonly usedin genetic engineering are:

A) Exonuclease and ligase C) Ligase and polymerase

B) Restriction endonuclease and polymerase D) Restriction endonuclease and ligase

111 A successful hybridoma was produced by fusing: 

A) Plasma cells and plasmids C) Myeloma cells and plasmids

B) Plasma cells and myeloma cells D) Plasma cells and bacterial cell

112 Plasmids are ideal vectors for gene cloning as: 

A) They can be multiplied by culturing Hyalauronidase

C) They can replicate freely outside the bacterial cell

B) They can be multiplied in the laboratory using enzymes

D) They are self replicating within the bacterial cell of the DNA

113 Self-replicating, small circular DNA molecules present in bacterial cell are known: 

A) Plasmids C) Plasmomeros

B) Cosmids D) Plastids

114 Monoclonal antibodies are produced by: 

A) Hybridoma technology C) Fermentation Technology

B) Biotechnology D) Biosynthesis

115 Enzymes are chemically: 

A) Lipids C) Carbohydrates

B) Proteins D) Fats

116 Hybridoma technique is used for:

A) Monoclonal antibodies C) Proteins

B) Polyclonal antibodies D) Antigens

117 Phyto-pharmaceuticals are produced from:

A) Bacteria C) Plants

B) Fungi D) Animals

118 Protoplast fusion involves:

A) Fusion of DNA C) Fusion of cells without cell wall

B) Fusion of nucleus D) Fusion of Cells with cell wall

119 Enzymes used in the Protoplast isolation:

A) Pectinase and Cellulase C) Amylase and pectinase

B) Catalase and Lipase D) Zymase and Amylase

120 Bio-pharmaceuticlas are:

A) Proteins or nucleic acids C) Amino acids or antibiotics

B) Lipids or carbohydrates D) Amino acids or fats

121 Biopharmaceuticals currently produced by genetic engineering in either:

A) E coli or animal cell C) Virus or animal cell

B) Plant or animal cell D) E coli or virus

122 Erythropoietin is secreted by:

A) Cells in macula densa C) Juxtaglomerular cells

B) Cells in the proximal tubules D) Cells in peritubular capillary bed

Unit- VI/Medicinal Plant Biotechnology and Animal Cell Technology 123 The enzymes required to obtain wall-free / naked protoplasts are:

A) Cellulase and proteinse B) Cellulase and Pectinase

C) Cellulase and Amylase D) Amylase and Pectinase

124 Polyethylene glycol is:

A) Fusogenic material B) Electro fusion stimulant

C) Callus stimulant D) differentiation stimulant

125 Plant tissue culture technique is a redefined method of:A) Vegetative propagation B) Asexual reproduction

C) Hybridization D) Selection

126 Somatic hybridization is achieved through:

A) Grafting B) Protoplast fusion

C) Conjugation D) Recombinant DNA technology

127 The first transgenic crop was:

A) Pea B) Tobacco

C) Flax D) Cotton

128 The technique of hybridoma formation is from:

A) Spleen cell – Myeloma cell B) Spleen cell – Spleen cellC) Myeloma cell – Myeloma cell D) kidney cell - Myeloma cell

129 The transgenic animals are those which have:

A) Foreign DNA in some of their cells B) Foreign DNA in all of

C) Foreign RNA in all of their cells D) Foreign RNA in some of their cells

130 Polyethylene glycol method is used for

A) Energy production from sewage B) Gene transfer without a vector

C) Seedless fruit production D) Biodiesel production

131 Which part would be most suitable for raising virus free plants for micropropagation

A) Meristem B) Node

C) Bark D) Vascular tissues

132 Transgenic animals are for the improvement in quality of

A)Milk B) Meat

C) Egg D) All the above

133 The first crop plant sequenced is,

A) Rice B) Tobacco

C) Maize D) Cotton

134 DNA finger printing was developed by

A) Francis Crick B) Khorana

C)Alec Jeffery D)James Watson

Unit-VII /Proteomics and Genomics

135 The proteome is

A) only useful to be studied in conjunction with the phenomena

B)refers to the entire complement of proteins

C) is what functional genomics is primarily interested in understanding

D) is now most commonly studied using RNA microarrays

136 A Map Unit refers to

A) the relative distance between genes on a chromosome

B) the chromosomes that exchange parts during meiosis

C) the percentage of recombination D) A and C

137 Genetics is the science of

A)Genes B) Heredity

C) the variation of organisms D) genes, heredity & the variation of organisms

138 Genomics is the study of

A)genetic patterns of genome of species B) genes

C) genes synthesizing proteins D) genes as diagnostic tools

E)

139 Proteomics is the study of

A)codons B) proteins

C) proteins and genes D) genes

140 Human genome project was completed in

A)January 2003 B) April 2013

C) April 2003 D) January 2013

141 Proteomics was coined by

A)Robert Koch B) Marc wilkins

C) Thomas Rederick D) Elizabeth Bugie

142 Genomics was coined by

A)Marc wilkins B) Alexander Fleming

C) Thomas Rederick D) Albert Schatz

Unit-VIII / Nanotechnology and Nanomedicine

143 Who coined the word 'nanotechnology'?

A) Eric Drexler B) Richard Feynmann

C) Sumio Tijima D) Richard Smalley

144 Which formulation contains nanoparticles prepared by using biologically processed metal ores.A)Homeopathic medicines B) Modern antibiotics

C) Ayurvedic 'Bhasmas' D) Modern cosmetics

145 The processing of separation, consolidation and deformation of materials by one atom or one molecule is called as:

A) Biotechnology. B) Physics.

C) Nanobiotechnology. D) Chemistry.

146 Which is the surface science and physical chemistry that focuses on fabrication of structures in carbon, silicon or other materials?

A) Wet nanobiotechnology B) Computational nanobiotechnology

C) Drug nanobiotechnology D) Nan biotechnology

147 Which metal is used with nanoparticles for antibiotic delivery?

A) Gold B) Titanium

C) Zinc D) Silver

148 Nano shells are used in the treatment of which of the following disease?

A) Alzheimer’s B) Cancer

C) HIV D) Parkinson’s

149 A Nano biological recognition component, which is involved in interacting with the analyte molecule is called as:

A) Biosensor B) Probe

C) Nanobiosensor D) Quantum Dots

150 Tiny semiconductor Nanoparticles with fascinating light-emitting properties are called as:

A) Nanoparticles B) Nanopores

C) Bucky Balls D) Quantum Dots