vito-abs offers toxicology and ecotoxicology services
TRANSCRIPT
12/02/2014
VITO, VIsion on TechnOlogy
Environmental Risk & Health (MRG) Team: Applied Bio & molecular Systems (ABS)
An Van Rompay and Jeroen Van Deun
2014
12/02/2014 2 © 2013, VITO NV
VITO, VIsion on TechnOlogy
» a leading European independent research and consultancy centre; » in the areas of cleantech and sustainable development; » elaborating solutions for the large societal challenges of today and
tomorrow.
12/02/2014 3 © 2013, VITO NV
More sustainable
industry
Reorganise our environment
Reduce fossil energy
dependency
Research on challenges of our society
Societal challenge
Economic opportunity
12/02/2014 4 © 2013, VITO NV
Themes
Sustainable Energy
Sustainable Chemistry
Sustainable Materials
Management
Sustainable Land Use
Sustainable Health
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Applied Bio & molecular Systems
WHO? WHAT? FOR?
9 Researchers (Biologist, Toxicologist, bio-engineers, statisticians)
6 Expert technicians 10 (Post-)docs
Toxicology Ecotoxicology
Government (Flanders, Belgium, Europe,…)
Industry
12/02/2014 6 © 2013, VITO NV
Toxicological assessments
ABS – Toxicological assessments
ECVAM validations Transfer of methods Performance assessment
Validation of alternative methods
Nanoparticles Omics (genomics and proteomics)
Sensitization Endocrine disruption
Neurodevelopment Embryotoxicity
R&D alternative methods & Biomarker discovery
Toxicity (Acute & chronic)
Biodegradation Water/soil/waste
CRO Ecotoxicity
testing
Skin corrosion Skin irritation
Skin Sensitization Eye irritation Genotoxicity Cytotoxicity
Endocrine disruption Inflammation responses
Embryotoxicity Neurodevelopment
CRO alternative
testing (replacing test
animals)
12/02/2014 7 © 2013, VITO NV
ABS – Quality labels • ISO 9001 = Quality • ISO 14001= Environment • OHSAS 18001 = Safety Good Laboratory Practice (GLP)
12/02/2014 8 © 2013, VITO NV
1200 m² of laboratory space: Operational Biological model systems for
human toxicology and ecotoxicology studies
Measurement devices
ABS – Facility
12/02/2014 9 © 2013, VITO NV
Toxicological assessments
ABS – Toxicological assessments
Skin corrosion Skin irritation
Skin sensitization Eye irritation Genotoxicity Cytotoxicity
Endocrine disruption Inflammation responses
Embryotoxicity Neurodevelopment
CRO alternative
testing (replacing test
animals)
12/02/2014 10 © 2013, VITO NV
Genotoxicity In vitro micronucleus test (OECD 487)* Ames II screen Ames MPF and Penta I test Comet test in human blood cells
Cytotoxicity Cell lines for safety or bio-compatibility testing (e.g. 3T3, Beas-2B, A549, THP-1, …) with multiple endpoints: NR-uptake, MTT, …
Skin sensitization VitosensTM test
Skin corrosion CorrositexTM test (OECD 435)* 3D-skin model RHE (OECD431)*
Skin irritation 3D-skin model RHE (OECD439)*
Developmental toxicity Zebrafish teratogenicity assay (larvae < 7 days)
Endocrine disrupting properties Human reporter cell lines (OECD 455, OECD 457)* hER transformed yeast cells
ABS – CRO Alternative testing
Eye irritation BCOP test (OECD 437)* 3D-eye model HCE test
Neurodevelopmental toxicity Zebrafish neurotoxicity assay (larvae < 7 days)
Inflammation responses Measurement of cytokines (e,g, IL-6, Il-8, Il-1β, TNF-α in cell lines
* These tests are approved for CLP and REACH
Endocrine disrupting tests only after license agreement
12/02/2014 11 © 2013, VITO NV
Toxicological assessments
ABS – Toxicological assessments
ECVAM validations Transfer of methods Performance assessment
Validation of alternative methods
12/02/2014 12 © 2013, VITO NV
ABS – Validation of alternative methods
• Ring tests (participation and organization) • EURL-ECVAM validations of alternative tests replacing test animals • Inter- and intra laboratory transfer of biological methods Examples of projects: • EU-Reprotect: validation of hEr and hAr cells – model for endocrine disruption
• Validation study of HCE (L’Oréal / EURL-ECVAM / Cosmetics Europe) – model for eye irritation
• Validation study of OS-REP (Henkel) – model for skin irritation
• Validation study of VITOSENS® in collaboration with Cosmetics Europe– model for skin sensitization
12/02/2014 13 © 2013, VITO NV
Toxicological assessments
ABS – Toxicological assessments
Toxicity (Acute & chronic)
Biodegradation Water/soil/waste
CRO Ecotoxicity
testing
12/02/2014 14 © 2013, VITO NV
Aquatic tests • Microtox test (ISO11348) • Algae test (OECD 201)* • Waterflea tests (OECD 202, 211)* • Fish tests (OECD 203, 204, 212, 236)*
Terrestrial tests • Microtox-SP test • Plant tests (OECD 208)* • Worm tests (OECD 207, 222)*
Biodegradation tests OECD 301b+d, 302*
ABS – CRO Ecotoxicity testing
* These tests are approved for CLP and REACH
12/02/2014 15 © 2013, VITO NV
Hazard characterization of Chemicals o Pharmaceuticals o Chemicals o Agrochemicals o Cosmetics o Veterinary drugs o Biocides o …
ABS – Application fields
Hazard profiling of food and environmental samples o Food
o Effluents
o Drinking water
o Ground and surface waters
o Waste water
o Soils and sediments
o Waste materials
o Air particles
o …
12/02/2014 16 © 2013, VITO NV
• Chemicals and products: o Testing according to GLP principles for registration purposes : ecotoxicity & in vitro
testing (CLP & REACH) for industry o Screening novel products for their intrinsic hazardous properties for industry
• Environmental assessment for industry and government: o Ecotoxicological effluent quality assessment as part of the environmental permit
conditions o Drinking water resources: looking for emerging compounds by the use of assays for
cytotoxicity, genotoxicity and endocrine disruption o Drinking water: sampling and looking for best purifications systems by the use of
genotoxicity o Air Particles: sampling and effect based measurements on particles and their extracts o Hazard characterization of complex waste materials by effect-based test methods o Hazard evaluation of industrial processes as a function of waste water quality to
direct/improve processes and waste water treatment and become a good end of pipe water quality
o Hazard characterization of excavated waste and air particles at landfill mining activities
ABS – Examples of projects