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Volume 26 August 1977 Journal of CELL SCIENCE CAMBRIDGE UNIVERSITY PRESS For the Company of Biologists Ltd

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Page 1: Volume 26 August 1977 Journal of CELL SCIENCE · Journal of Cell Science is devoted to the study of cell organization. Papers will be published dealing with the structure and function

Volume 26 August 1977

Journal of CELLSCIENCE

CAMBRIDGE UNIVERSITY PRESSFor the Company of Biologists Ltd

Page 2: Volume 26 August 1977 Journal of CELL SCIENCE · Journal of Cell Science is devoted to the study of cell organization. Papers will be published dealing with the structure and function
Page 3: Volume 26 August 1977 Journal of CELL SCIENCE · Journal of Cell Science is devoted to the study of cell organization. Papers will be published dealing with the structure and function

Journal of Cell Science is devoted to the study of cell organization. Papers will be publisheddealing with the structure and function of plant and animal cells and their extracellular products,and with such topics as cell growth and division, cell movements and interactions, and cellgenetics. Accounts of advances in the relevant techniques will also be published. Contributionsconcerned with morphogenesis at the cellular and sub-cellular levels will be acceptable, as willstudies of micro-organisms and viruses, in so far as they are relevant to an understanding of cellorganization. Theoretical articles and occasional review articles will be published.

Subscriptions. The Journal of Cell Science is published six times a year, in February, April, June,August, October and December. Each issue is a complete volume. The subscription price ofvolumes 23-28, 1977, is £54.00 (U.S.A. and Canada US $135.00) post free. Individual vol-umes may be purchased at £10.00 (U.S.A. and Canada US $23.50) each, plus postage. Ordersmay t»e sent to any bookseller or subscription agent, or direct to Cambridge University Press,Bentley House, 200 Euston Road, London NWi 2DB or in the U.S.A. and Canada to CambridgeUniversity Press, 32 East 57th Street, New York, N.Y. 10022, U.S.A. Copies of the journal forsubscribers in the U.S.A. and Canada are sent by air to New York for delivery with the mini-mum delay. Second class postage paid at New York, N.Y.

Back numbers of the Journal of Cell Science are available from Cambridge University Press, at theaddresses given above. This journal is the successor to the Quarterly Journal of MicroscopicalScience, back numbers of which are obtainable at £8.00 per volume from Messrs William Dawson& Sons, Cannon House, Folkestone, Kent, U.K.

Enquiries about the reproduction of material from Journal of Cell Science should be sent to:Dr R. J. Skaer, Secretary, Company of Biologists Limited, Department of Zoology, DowningStreet, Cambridge CB2 3EJ, U.K.

ISI Tear Service, 325 Chestnut Street, Philadelphia, Pennsylvania 19106, U.S.A., is authorizedto supply single copies of separate articles for private use only.

© Company of Biologists Limited 1977

Page 4: Volume 26 August 1977 Journal of CELL SCIENCE · Journal of Cell Science is devoted to the study of cell organization. Papers will be published dealing with the structure and function
Page 5: Volume 26 August 1977 Journal of CELL SCIENCE · Journal of Cell Science is devoted to the study of cell organization. Papers will be published dealing with the structure and function

INFORMATION FOR CONTRIBUTORS

i Manuscripts should be sent to The Editors,Journal of Cell' Science, Department of Zoology,Cambridge CB2 3EJ, England.

a Manuscripts must be typewritten, in doublespacing throughout (including tables, references andlegends). Each table should be typed on a separatesheet. Legends to figures should be typed in asingle series and placed at the end of the manuscript.Papers must be fully corrected by the author, and acharge will be made for excessive alteration in proof.

3 A short title of not more than 40 characters, foruse as page headings, should be supplied if the fulltitle is longer than this.

4 Manuscripts must contain a Summary of notmore than 500 words, placed immediately after thetitle page. Contributors should also send two copiesof an Abstract for distribution to abstrating journals.The abstract must not be more than 100 words longand should be headed by the author's name andaddress and the title of the paper. Both summaryand abstract must be intelligible without referenceto the main text.

5 The list of References must be given inalphabetical order of authors' names. The titles ofjournals should be abbreviated in accordance withthe World List of Scientific Periodicals, 4th ed. (1963).The following style is used:

GRAY, E. G. & WILLIS, R. A. (1968). Problems ofelectron stereoscopy of biological tissue. J. CellSci. 3, 309-326.

MAZIA, D. (1961). Mitosis and physiology ofcell division. In The Cell, vol. 3 (ed. J. Brachet& A. E. Mirsky), pp. 77-412. New York andLondon: Academic Press.

Citations in the text are given in the following form:Jones & Smith (i960) or (Jones & Smith, i960).Where there are more than two authors the firstcitation should include all the names and subsequentcitations should be in the form (Jones et al. i960).Where more than one paper by the same author(s)

have been published in the same year they are citedas Jones (1960a), Jones (19606) etc.

6 Footnotes should be avoided wherever possible.

7 SI units should be used throughout in thepreparation of manuscripts.

8 Text figures and photographs should benumbered in a single series, in the order in whichthey are referred to in the text. Each individualdrawing or photograph should be numberedseparately (Fig. 1, Fig. 2 and so on).

9 Text figures should preferably be drawn abouttwice final size; very large drawings should beavoided. Original drawings are needed for reproduc-tion as photographic copies cannot always be used.The maximum printed size of a drawing is 200 mmby 125 mm. Lettering will be inserted by the printersand should be indicated on a tracing-paper overlayor a duplicate copy.

10 Photographs should preferably be submittedthe same size as they are to appear. The maximumfor a plate is 200 mm by 140 mm. Where severalphotographs make up a plate they should beaccurately mounted on one sheet of cardboard andirregular shapes avoided wherever possible. Letteringon plates will be inserted by the printers and shouldbe indicated either on a duplicate, marked set ofprints or on a tracing-paper overlay bearing accu-rately marked outlines of the objects indicated.Authors may be asked to contribute the cost of platesin excess of four.

n Authors will receive 50 offprints free of chargeand may order additional copies when proofs arereturned.

12 In order to give the Company of Biologistsauthority to deal with matters of copyright, authorswill be required to assign to them the copyright ofany article published in the journal. Authors willnot, however, need to ask permission to re-use theirown material in other publications appearing undertheir own name.

Page 6: Volume 26 August 1977 Journal of CELL SCIENCE · Journal of Cell Science is devoted to the study of cell organization. Papers will be published dealing with the structure and function
Page 7: Volume 26 August 1977 Journal of CELL SCIENCE · Journal of Cell Science is devoted to the study of cell organization. Papers will be published dealing with the structure and function

Published by the Syndics of the Cambridge University PressThe Pitt Building, Trumpington Street, Cambridge CB2 iRP

Bentley House, 200 Euston Road, London NWi 2DB32 East 57th Street, New York, NY 10022, USA

Company of Biologists Limited 1977

Printed in Great Britain at the University Press, Cambridge

Page 8: Volume 26 August 1977 Journal of CELL SCIENCE · Journal of Cell Science is devoted to the study of cell organization. Papers will be published dealing with the structure and function

CONTENTS VOLUME 26 AUGUST 1977

page i HEYWOOD, PETER. Evidence from serial sections that some cells contain largenumbers of mitochondria

9 MURRAY, D. R. and KNOX, R. B. Immunofluorescent localization of urease inthe cotyledons of jack bean, Canavalia ensiformis

19 STEWART, P. and PITT, D. Ultrastructural localization of acid phosphataseactivity in root tips by the />-(acetoxymercuric) aniline diazotate reagent anda comparison with a Gomori procedure

31 LARKIN, P. J. Plant protoplast agglutination and membrane-bound /9-lectins47 SCHAEFFER, BARRY E. and CURTIS, A. S. G. Effect9 on cell adhesion and

membrane fluidity of changes in plasmalemmal lipids in mouse L929 cells57 ALBERTINI, D. F., BERLIN, R. D. and OLIVER, J. M. The mechanism of

Concanavalin A cap formation in leukocytes77 WAHRMANN, J. P., RECOUVREUR, M. and FAVARD-SERENO, C. Development and

regulation of the phosphorylase-glycogen complex in myogenic cells of the L6 line93 OZATO, KENJIRO. Mitotic activity of differentiated goldfish erythrophores in

culture101 VAN DER SCHUEREN, BERNADETTE, CASSIMAN, JEAN-JACQUES and VAN DEN BERGHE,

HERMAN. Outgrowth of human fibroblast aggregates on a substratum triggersa wide variety of morphogenetic properties in the cells

119 CONRAD, GARY W., HART, GERALD W. and CHEN, YEH. Differences in vitrobetween fibroblast-like cells from cornea, heart, and skin of embryonic chicks

139 TICKLE, C. and TRINKAUS, J. P. Some clues as to the formation of protrusionsby Fundulus deep cells

151 TAKEUCHI, YUKO and YONEDA, MITSUKI. Synchrony in the rhythm of thecontraction-relaxation cycle in two plasmodial strands of Physarum polycephalum

161 WIGGLESWORTH, V. B. Structural changes in the epidermal cells of Rhodniusduring tracheole capture

175 LANE, NANCY J., SKAER, HELEN L E B. and SWALES, LESLEY S. Intercellularjunctions in the central nervous system of insects

201 TAPP, R. L. and HOCKADAY, AUSTIN. Combined histochemical and X-raymicroanalytical studies on the copper-accumulating granules in the mid-gut oflarval Drosophila

217 HALKKA, LIISA and HALKKA, O. Accumulation of gene products in the oocytesof the dragonfly Cordulia aenea. II . Induction of annulate lamellae within densemasses during diapause

229 SOLARI, ALBERTO J. and COUNCE, S. J. Synaptonemal complex karyotyping inMelanoplus differential's

251 SKAER, R. J. Interband transcription in Drosophila267 DAVIES, K. E. and WALKER, I. O. In vitro transcription of RNA in nuclei,

nucleoli and chromatin from Physarum polycephalum281 STACK, STEPHEN M., BROWN, DAVID B. and DEWEY, WILLIAM C. Visualization

of interphase chromosomes301 SKAER, R. J. and WHYTOCK, SUSAN. Chromatin-like artifacts from nuclear sap

311 MORRILL, GENE A., ZIEGLER, DAVID and ZABRENETZKY, VIVIAN S. An analysis oftransport, exchange, and binding of sodium and potassium in isolated amphibianfollicles and denuded oocytes

323 WASSARMAN, P. M., UKENA, T . E., JOSEFOWICZ, W. J., LETOURNEAU, G. E. andKARNOVSKY, M. J. Cytochalasin B-induced pseudocleavage of mouse oocytesin vitro. II . Studies of the mechanism and morphological consequences ofpseudocleavage

Page 9: Volume 26 August 1977 Journal of CELL SCIENCE · Journal of Cell Science is devoted to the study of cell organization. Papers will be published dealing with the structure and function

iv Contents

339 FREDERIK, P. M., VAN DER MOLEN, H. J., KLEPPER, D. and GALJAARD, H.Electron-microscopic autoradiography of tritiated testosterone in rat testis

347 ALTER, BLANCHE P., GOFF, SABRA C, HILLMAN, DIANE G., DEISSEROTH, ALBERT B.and FORGET, BERNARD G. Production of mouse globin in heterokaryons ofmouse erythroleukaemia cells and human fibroblasts

359 SCHUSTER, F. L. and CLEMENTE, JOHN S. 5-Bromodeoxyuridine-inducedformation of virus-like particles in Naegleria gruberi EGB

373 ROSIN, MIRIAM P. and ZIMMERMAN, A. M. The induction of cytoplasmic petitemutants of Saccharomyces cerevisiae by hydrostatic pressure