Weight Management Gene Expression Science

Advances in Gene Expression Science Allow Scientists to Compare & Contrast

Expression Patterns of two Biological States

Biological State #1 Biological State #2 State

Comparison of Genetic Profiles of Adipose Tissue in Identical Twins

Pietiläinen et al. PLoS Med. 2008 Mar 11;5(3):e51.

Same DNA

Different Expression of Genes

Normal Weight Twin Overweight Twin

Compared to their normal weight twin, overweight twins exhibited down regulation of gene expression pathways related to:

• Fat Regulation (storage versus utilization)• Muscle Protein Synthesis

• Nu Skin owns patented technologies to study anti-aging interventions through gene expression science

• Deep understanding of gene expression science

Identified Gene Expression Pathways Linked toFat Storage and Fat Metabolism

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LifeGen pre-clinical study of Adipose, Gastrocnemius muslce, Hypothalamus

Ghrelin

LeptinInsulin

References:

Gene Expression relates to the Appetite Control Center of the Brain

• Appetite Control• Eating Behavior• Regulation of energy intake

1. Smeets et al. Proceeding of the Nutrition Society. 2012;71:511-520.

2. Guthoff M, et al. J Clin Endocrinol Metab. 2010;95:748-755.

3. De Silva, et al. Cell Metab. 2011;14:700-706.

4. Malik S, et al. Cell Metab. 2008;7:400-409.

5. Farooqi IS, et al. Science. 2007;317:1355

6. Suzuki K, et al. Experimental Diabetes Research. 2012:1-19.

7. Viquerie N, et al. Diabetologia. 2005;48(1):123-131.

8. Mager et al. European Journal of Endocrinology. 2008;158:

9. Mutch DM, et al. Am J Clin Nutr. 2011;94(6):1399-1409.

Formulations Based on:• Proprietary gene Expression Research• The latest and most robust nutritional science

Eating Plan Based on:Protein Science & Nutrition Science

• Supports metabolically-active lean muscle and inhibits fat storage1

Reference: Wycherley TP, et al. AJCN. 2012;96(6):1281-98.

• An eating plan designed to complement the supplements.

Carbohydrates(260g)

Proteins(90g)

Fats(67g)

Typical Reduced Calorie Diet – 2000 kcalF

at Burning

Foods

Fat

Sto

ring

Foo

ds

Fat S

toring F

oods

Carbohydrates(260 g)

Proteins(90 g)

Fats(67 g)

Carbohydrates(260g)

Proteins(90g)

Fats(67g)

Typical Reduced Calorie Diet – 2000 kcalF

at Burning

Foods

Fat

Sto

ring

Foo

ds

Fat S

toring F

oods

Carbohydrates(180 g)

– 1200 kcal

Fats(33 g)

Proteins(45 g)

Carbohydrates(260g)

Fats(67g)

Proteins(90g)

Eating Plan – 1200 kcalF

at Burning

Foods

Fat

Sto

ring

Foo

ds

Fat S

toring F

oodsProteins

(90 g)

Carbohydrates(260 g)

Fats(67 g)

Carbohydrates(260g)

Proteins(90g)

Fats(67g)

Fat B

urning F

oods

Fat

Sto

ring

Foo

ds

Fat S

toring F

oods

Carbohydrates(120 g)

Fats(40 g)

Eating Plan – 1200 kcal

Proteins(90 g)

Eating Most of Your Protein with Only One Meal of the Day Limits Duration of Metabolic Burn

FA

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Time in Metabolic Burn Zone = Only 3 hours/day

Adapted from: 1. Paddon-Jones and Rassmussen. Curr Opin Clin Nutr Metab Care, 2009;12:86.2. Layman DL. Nutrition and Metabolism, 2009; 6:12.

Breakfast Lunch Dinner

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Eating Protein with Each Meal Accelerates Metabolic Burn

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Adapted from: 1. Paddon-Jones and Rassmussen. Curr Opin Clin Nutr Metab Care, 2009;12:86.2. Layman DL. Nutrition and Metabolism, 2009; 6:12.

Breakfast Lunch Dinner

Time in Metabolic Burn Zone = 9 hours/day

ZO

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Open Label Clinical Study

Participants Reported…- Lower levels of Hunger- Changes in body silhouette- Clothes fit better- Liked this program better than programs

they had tried previously

- 90 Days on ingredients-based program- 45 women and 21 men completed the study

Results of unpublished preliminary study 2013

ageLOC TR90 Summary• Healthy weight management and Body Shaping program.• Based on gene expression science, addresses key

factors related to weight management: - Healthy Metabolism, - Promotes Lean Muscle Maintenance - Appetite Control

• Studies on TR90 weight management program resulted in significant weight loss, fat loss and changes in gene expression.

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SIGNS & SYMPTOMS

NutritionScience

Gene Expression

Science

Antioxidant Science

LIFEPAK—THE PERFECT COMPANION

• Nutritional foundation is important– LifePak® provides that foundation

• Ensure adequate nutrition during reduced calorie regimen

• Dietary antioxidants for added protection from oxidative stress

LifePak Off-sets Oxidative Stress During DietingAv

erag

e SC

S fo

r stu

dy

parti

cipa

nts

10,000

20,000

30,000

40,000

36,00030,00029,000

Baseline 6 Weeks 12 Weeks

• Participants took LifePak

• Scanner Scores increase an average of 6,000 per person

Data from My Victory Open-label Study 2007

AGELOC R2 – The Perfect Companion

• Maintain energy levels

• Assist with cell renewal and cleansing during lipolysis

TR90 SUPPORT TOOLS

Create and communicate your personal transformation through a compelling story and transformation number that is completely unique to your ageLOC TR90 experience.

WHAT’S YOUR TRANSFORMATION NUMBER? Ninety nine