workshop manual and souvenir.pdf

National Seminar cum

Workshop on

National Seminar cum Workshop on

Applications of Green Technologies for the

Improvement of Agrobased System in India

- A Biotechnological Approach




Sponsored by:

Department of Biotechnology, Govt. of India, New Delhi

SERB, Govt. of India, New Delhi

NABARD, Govt. of India, Mumbai

Sponsored by:




Department of BiotechnologyDepartment of Biotechnology

Karpaga Vinayaga Karpaga Vinayaga College of Engineering and TechnologyCollege of Engineering and Technology

Chinna Kolambakkam, Padalam - 603308Madhuranthagam (Tk.), Kanchipuram (Dt.), TN


A Manual onPreparation of Organic Fertilizers

&

Souvenir


&

Souvenir

rd th23 & 24 July 2015

rd th23 & 24 July 2015

National Seminar cum Workshop on




Sponsored by:




Department of Biotechnology

Karpaga Vinayaga College of Engineering and Technology



&

Souvenir

rd th23 & 24 July 2015

Mrs. Meenakshi Annamalai, M.Sc. (Ph.D)

Director

Karpaga Vinayaga College of

Engineering and Technology

Message

I am happy to note that department of Biotechnology in

Karpaga Vinayaga College of Engineering and

Technology is Organizing a National Seminar cum

Workshop on “National Seminar cum workshop on

“Applications of Green Technologies for the

Improvisation of Agro Based System in India – A

Biotechnological Approach” on 23rd and 24th July, 2015

funded by DST, SERB and NABARD and going to

release the proceeding during this occasion

For past decades KVCET had been rendering Quality

service in the field of Engineering Education by

providing excellent Infrastructure Facilities and the right

team of well qualified staff. This makes KVCET to march

towards confidently in this competitive era.

I express my whole hearted congratulations on this

occasion to all the staff & students of Biotechnology

departments and wish the Seminar cum Workshop a

grand Success.

Director

Dr. SM. Kannan, Ph.D

Principal



Message

Warm and Happy greeting to all. I am immensely happy

that Biotechnology department of our college is

organizing a national Seminar cum workshop on

Applications of green Technologies for the improvisation

of agro based system in India – A biotechnological

approach on 23rd and 24th July, 2015 funded by DST,

SERB and NABARD and is going present a collection of

various technical papers in the proceedings.

Under the able guidance of our management KVCET

continues to march on the way of success with

confidence. The sharp, clear sighted vision and precise

decision making of our management has made the

college and students competitive.

I also congratulate HOD, staff members, students of

Biotechnology department, Participants for their efforts

in organizing and participating in this seminar cum

workshop and I wish them all the success.

Principal

Dr. VC. Ravichandran, Ph.D

Advisor



Message

I am extremely happy to understand that the Department

of Biotechnology is organizing a National Seminar cum

workshop on Applications of green Technologies for the

improvisation of agrobased system in India – A

biotechnological approach on 23rd and 24th July, 2015

funded by DBT, SERB and NABARD.

The applications of many advanced science and

engineering are to help the nation for its development.

Biotechnology in its present day scenario has a

challenge to provide reliable natural sources to farmers

from the green technology.

It is essential for those professionals of Biotechnology to

get new updates of energy and its advanced

applications to meet the challenges.

I hope this seminar cum workshop would certainly

induce innovative ideas among the participants paving

way for new inventions and new technologies in the

Biotechnology discipline.

Advisor

Dr. Sultan Ahmed Ismail

Director

Ecoscience Research Foundation

Chennai

Message

Earthworms have always been a subject of interest and

many ancient scientists, sages and poets have sung its

praises. While the Tamil poet Manonmanian

Sundaranar has extolled the earthworms as “those who

can remediate any type of difficult soil”, scientists have

praised the earthworms either as “the intestines of the

earth” (Aristotle) or as “nature‟s plough” (Darwin).

Earthworms have always been regarded as friends of

the farmers. Earthworms have a very positive effect on

the physical, chemical and biological parameters of the

soils. The earthworm is a soil biotechnologist and a solid

waste manager. Earthworms are known to consume

large quantities of organic litter or waste and convert

them into manure, which is used as valuable compost,

known as „vermicompost‟.

The significance of earthworms in soils is manifold.

They are unique among soil invertebrates as they bury

organic debris, mix organic and inorganic matter in their

excreta and modify the structure of the soil through their

burrow systems. Their importance in nutrient cycling,

soil structure, soil health including soil fertility,

productivity, agriculture, and their application in organic

waste management has been well established.

Therefore the earthworm is considered as the leader in

revitalization of the soil.

Though a group of beneficial organisms such as

Bacteria, fungi, actinomycetes, protozoans,

microarthropods (proturans, spring-tails,

pseudoscorpions, pauropods, symphylids), millipedes,

centipedes, slugs, snails, snakes, rodents, and a host of

flora join the earthworms in maintaining soil health, the

presence of earthworms is an indicator of soil health. To

diagnose soil health one has to feel the pulse of the soil.

Earthworms are the pulse of the soil. Healthier the

pulse, healthier is the soil.

I sincerely congratulate the Department of

Biotechnology, Karpaga Vinayaga College of

Engineering and Technology, Maduranthagam, for

organizing the National Workshop on "Application of

Vermicomposting Practices for Sustainable

Agricultural Development" sponsored by Department

of Biotechnology, Government of India, and wish the

team all success.

Sultan Ahmed Ismail, Ph.D, D.Sc.,

Dr. V. Karthikeyan HOD & Organizing Secretary Department of Biotechnology Karpaga Vinayaga College of


Message

It is with immense pride and anticipation that I invite

everyone to attend the National Seminar cum workshop

on Applications of green Technologies for the

improvisation of agro based system in India – A

biotechnological approach on 23rd and 24th July, 2015

funded by DST, SERB and NABARD.

The Workshop will bring forth cutting-edge topics and

distinguished experts as speakers to foster advanced

studies as well as lively discourse between hundreds of

expected delegates from different parts of the nation.

The Workshop will look forward to the advancement in

Organic farming practices to the farmers in our country.

This Workshop will be familiar and refreshing charms.

Head & Organizing Secretary


CONTENTS

Particulars Page No.

1. Panchakavyam 01

2. Amirtha karaisal 04

3. Meen Paagu 06

4. Bio Pesticide 08

5. Curd-Herbal Syrup 11

6. Cow Horn Manure 13

7. Cow Pat Pit 15

A Manual on Preparation of Organic Fertilizers

Sponsored by: DBT, SERB, NABARD 1

1. PANCHAKAVYAM

INGREDIENTS:

Cow Dung 5 kg

Cow’s Urine 3 litres

Cow’s Milk 2 litres

Curd 2 litres

Ghee 1 litre

Tender Coconut Water 2 litres

Jaggary 1 kg

Banana 10 Nos

Yeast 25g

Hone 1/2 litres.



PREPARATION:

Mix equal amounts of fresh cow dung and

ghee into a good consistency.

Store it at room temperature for 3 days[52

hours].

Pour the contents into a 25 litre plastic drum.

Mix well during morning and evening. [By

doing this the methane gas will get liberated].

During the fourth day add the remaining

contents namely milk, curd, tendercoconut

water, banana, honey and yeast.

Mix thoroughly until it reaches watery

consistency. Close the drum.

Mix the contents daily by swirling the contents

in clockwise and counter clockwise direction

for atleast 15 days.



METHOD OF USAGE:

Add 300 ml of the preparation mix to 10 litres of

water and sprinkle on the crops every morning or

evening.

For 1acre

around 100 litres of water is required.

3 litres of Panchakavya is essential.

For a 20 day interval,sprinkle the above mentioned

preparation 2 or 3 times to get best results.

Additionally, add 50g of Khadhi soap in water and

sprinkle it on the crops. It enhances crop growth

besides its potent role as a pesticide.



2. AMIRTHA KARAISAL

[JEEVA-AMIRTHAM]

INGREDIENTS:

200 litres Capacity plastic drum

Water 200 litres

Cow dung 10 kg


Jaggary 1 kg

Powdered pulses 2kg

Wet soil Handful

PREPARATION:

Add 10 kg of fresh cow dung, 10 litres of

cow’s urine, jaggary and powdered pulses to

200 litres of water.



Add handful of soil to it.Mix thoroughly for a

few days.

The preparation will be ready in 3 days.

METHOD OF USAGE:

Add 1 litre of the above preparation to 10 litres of

water and sprinkle it on the crops.

APPLICATION:

It increases the growth of microbes in the soil.

The roots of the crops will become dense.

The growth of Azospirillum, Rhizobium,

Acetobacter, Pseudomonas viridae and

Phosphobacteria will be enhanced.



3. MEEN-PAAGU [FISH AMINO ACID]

INGREDIENTS:

2 litre capacity plastic drum

Fish 1kg

Jaggary 1kg

Tender coconut water 1litre

PREPARATION:

Cut the fishes into small pieces and place them

in a clean vessel.

Add finely grated jaggary and tender coconut

water to it and mix well. Close the vessel

properly.

Allow it to stand undisturbed for 30 days.



Then, filter the contents carefully with a clean

nylon cloth and collect the filtrate .Collect it in

an air tight bottle.

The remaining contents {Fish pieces} can be

reused by repeating steps 1 and 2.Allow the

contents to stand for 15 to 20 days.Filter the

contents and collect the filterate.

METHOD OF USAGE:

Add 50 ml of the above preparation in 10 litres of

water.

Sprinkle them on all kinds of crops during

evening.

Sprinkling can be done on the crops once in 20

days.



4. BIO-PESTICIDE

INGREDIENTS:

100 litre capacity plastic drum

Neem leaves 1kg

Pongamiagladira leaves 1kg

Nochi leaves 1kg

Erukkam leaves 1kg

Pirandai leaves 1kg

Cow Dung 5kg

Cow’s Urine 10litres

Jaggary 1kg

Rag Bags 2 Nos



PREPARATION:

The above mentioned leaves are ground well

and tied properly in rag bags.

Take another rag bag and fill it with fresh cow

dung.

Add 90 litres of water, 10 litres of cow’s

urine,1 kg jaggary in a large plastic tankand

mix well.

Lower the two rags bags into the plastic tank

such that they are completely submerged in the

slurry.

Mix the contents thoroughly for 15 days

METHOD OF USAGE:

Take 1 litre of the above preparation in 10 litres of

water and sprinkle on the crops once In 25 days.



APPLICATION:

It acts as a pesticide and increases the growth rate

of the plants.



5. HERBAL SYRUP

INGREDIENTS:

Powdered Neem seed 1 Kg

Curd 2 litres


Kaddukai Powder 25g

Adhimaduram Powder 25g

Fenugreek Powder 25g

Water 5 Litres

PREPARATION:

Soak neem seeds by tying them using a clean

piece of cloth for 24 hours.

Filter the contents carefully.



To the filtrate add 3 litres of cow’s urine, 2

litres curd, ‘kaddukai’ powder,’adhimaduram’

powder and fenugreek powder. Mix well and

store it for 7 days.

METHOD OF USAGE:

1 litre of the preparation is mixed with 10 litres of

water.

During flowering time, sprinkle it on the crops

during morning and evening.

APPLICATION:

It enhances/induces flowering of crops.



6. COW HORN MANURE (BD 500)

Cow horn-manure, a humus mixture prepared

by filling the horn of a cow with cow manure

and burying it in the ground (40–60 cm below

the surface) and covered with good quality

compost. The pit should be kept cool by

mulching with debris, coconut fronds etc., the

preparation was ready after three- four months.

It is used at the rate of 25 grams in 13 litres of

water and stirred for one hour making a vortex

then be sprinkled with a broom sticks. With

regular application of BD 500 will give a soil to

improve humus formation, soil tilth, earthworm

activity.



7. COW PAT PIT

Cow pat pit known as CPP, is specialized type

of compost. It refers to cow manure mixed with

crushed egg shell and rock dust. Cow pat pit

(CPP) structures were constructed and BD

preparations (BD 502-507 ) inoculated in Cow

dung, egg shell and rock at the rate of 60kg,

200g, 300g respectively.CPP was turned once

every 15 days and moisture content maintained

by sprinkling water. Use as a ground

application at 1kg per acre CPP to 40 litres of

water and stir for 10 minutes. It contains a wide

range of microbial population, growth

promoting hormones, which can be very

helpful in many areas of agricultural and

horticulture.

nghUslf;fk;

1. gQ;rft;ak; 01

2. mkpHj fiury; 04

3. kPd; ghF 07

4. jhtu G+r;rptpul;b 09

5. japH %ypif fiury; 11

6. khl;L nfhk;G cuk; 13

7. %ypif rhz cuk; 15

A Manual on Preparation of organic fertilizers


1. gQ;rft;ak;

NjitahdnghUl;fs;:-

1. gRtpd; rhzk; - 5 fpNyh

2. gRtpd; rpWePH(Nfhkpak;) - 3 ypl;lH

3. gRtpd; ghy; - 2 ypl;lH

4. gRtpd; japH - 2 ypl;lH

5. gRtpd; nea; - 1 ypl;lH

6. ,sePH - 3 ypl;lH

7. nty;yk; - 1 fpNyh (3ypl;lH

jz;zPhpy; fiuj;Jf; nfhs;sNtz;Lk;)

8. thiog;gok; - 12 (1 l[d;)

9. <];l; - 25 fpuhk;

10. Njd; - miuypl;lH

jahhpf;Fk; Kiw:-

rhzk;> nea; ,uz;ilAk; ed;F gpire;J

fyf;fTk;. ,ij 52 kzpNeuk; (3 ehs;)



KOtJk; itj;jpUf;fTk;. gpwF 25 ypl;lH

gpsh];bf; buk;kpy; Nghl;L itf;fTk;. fhiy>

khiy ,uz;L NtisfspYk; ed;F gpire;J

fyf;fTk;. ,jpy; cs;s kPj;Njd; thA

ntspNawptpLk;. gpwF ehd;fhtJ ehs; Nkw;gb

nrhy;yg;gl;l nghUl;fs; mjhtJ> ghy;> japH>

,sePH>thiog;gok; (gpire;jJ)> Njd;> <];l;

NrHf;fTk;. ,it midj;Jk; jz;zPH gjj;jpw;F

tUk; tiu ePH NrHj;Jnfhs;sTk;. buk;ik %b

itf;fTk;. jpdKk; fhiy khiyNtisapy;

Fr;rpahy; 50 Rw;Wfs; tyJ> ,lJ Gwkhf

fyf;fp Rw;wTk;. njhlHe;J 15 ehl;fs;

nra;aNtz;Lk;.

gad;gLj;Jk; Kiw:-

10 ypl;lH jz;zPhpy; 300 kpyp fye;J fhiy

my;yJ khiyNtisapy; gapHfs; kPJ

njspf;fNtz;Lk;. Vf;fUf;F 10 Nlq;f; (100



ypl;lH jz;zPH) Njitg;gLk;. 3 ypl;lH

gQ;rft;ak; Njitg;gLk;. gapHfSf;F 20 ehl;fs;

,ilntspapy; ,uz;L my;yJ %d;W jlit

Njitf;Nfw;g njspf;fTk;. fhjpNrhg;G 50 fpuhk;

jz;zPhpy; fye;J xl;Lk; jputkhfTk;

gad;gLj;jyhk;.

,J XH rpwe;j gapH tsHr;rp Cf;fpahFk;.

G+r;rptpul;bahfTk; gad;gLj;jyhk;.

----------------------------------------------------------------



2. mkpHj fiury; ([PthmkpHjk;)

NjitahdnghUl;fs;:-

1. 200 ypl;lH nfhs;ssT nfhz;l gpsh];bf;

buk;

2. jz;zPH - 200 ypl;lH

3. gRtpd; rhzk; - 10 fpNyh

4. gRtpd; rpWePH (Nfhkpak;) - 10 ypl;lH

5. nty;yk; - 1fpNyh

6. gapHtifkhT

(fliykhT my;yJ cSe;JkhT - 2 fpNyh

7. epyj;jpd; <u kz; (tug;G Xuj;jpy; ,Uf;Fk;

kz;) xUifg;gpb

jahhpf;Fk; Kiw:-

200 ypl;lH jz;zPhpy; gj;J fpNyh rhzk;>

gj;Jypl;lH Nfhkpak;> nty;yk;> gapHtif khT

NrHj;J jz;zPhpy; fyf;fTk;. epyj;jpd;

ifg;gpbkz;izAk; NrHf;fTk;. gpwF jpdKk;



fhiy khiy Fr;rpahy; fyf;fTk;. 3 tJehs;

mkpHjfiury; jahuhfptpLk;.

gad;gLj;Jk; Kiw:-

gj;J ypl;lH jz;zPhpy; xU ypl;lH mkpHjfiury;

fye;J gapHfSf;F njspg;ghd; nfhz;L

njspf;fyhk; my;yJ jz;zPH ghAk; NghJ

tha;f;fhypy; fye;Jtplyhk;.



gad;fs;:-

epyj;jpy; Ez;Zaphpfs; ngUf;fkilAk;.

mlHj;jpahf NtHkz;lyk; cUthFk;. ,J

jior;rj;ij epiyg;gLj;j $baJ.

mNrh];ighpy;yk;> iuNrhgpak;> mrpl;Nlhghf;lH

Nghd;w Ez;Zaph;fs; kw;Wk; gh];Nghghf;Bhpah>

#NlhNkhdh]; tphpb Nghd;w Ez;ZapHfs;

epiwe;J fhzg;gLk;.

----------------------------------------------------------------



3. kPd; ghF (kPd; mkpNdhmkpyk;)

NjitahdnghUl;fs;:-

1. ,uz;L ypl;lH gpsh];bf; buk;

2. kPd; -1fpNyh

3. nty;yk; -1fpNyh

4. ,sePH -1ypl;lH

jahhpf;Fk; Kiw:-

kPid rpWJz;Lfshf ntl;b buk;kpw;Fs;

NghlTk;. ed;F nghb nra;j nty;yk; kw;Wk;

,sePH Mfpatw;iw ,jDld; NrHj;J Fr;rpahy;

fyf;fp %lTk;. ,ij 30 ehl;fSf;F

nehjpf;ftplTk;. xU khjk; fopj;J ,ij

tbfl;lTk;. Njd; Nghd;w ghF fiury;

fpilf;Fk;. ,ij xUghl;bypy;

Nrkpj;Jitf;fTk;.



,t;thW nra;j gpd;dH KOikahf rpijahj

kPd; Jz;Lfs; ,Uf;f tha;g;G cz;L. ,jpy;

Njitahd msT nty;yk;> ,sePH Nghd;wtw;iw

NrHj;J NkNy Fwpg;gpl;l Kiwapy; Fr;rpahy;

fyf;fp gj;J ehl;fs; my;yJ gjpide;J ehl;fs;

itj;J ,Uf;fTk;. gpd;dH tbfl;b Kjypy;

gpope;j kPd; ghFld; NrHj;J itj;Jnfhs;sTk;.

gad;gLj;Jk; Kiw:-

gj;J ypl;lH jz;zPhpy; 50 kpyp kPd; fiury;

NrHj;J khiy Ntisapy; vy;yh tifahd

gapHfSf;Fk; njspf;fyhk;. ,e;j kPd; fiuriy

20 ehl;fs; ,ilntspapy; gapHfspd; Nky;

njspg;gjhy; A+hpah Nghl;lJ Nghd;W gRikahf

,Uf;Fk;.

----------------------------------------------------------------



4. jhtu G+r;rptpul;b

NjitahdnghUl;fs;:-

1. 100 ypl;lH nfhs;ssT cs;s gpsh];bf;

buk;

2. Ntg;ge;jio -1fpNyh

3. Gq;fd;jio -1 fpNyh

4. nehr;rpj;jio -1 fpNyh

5. vUf;fd;jio -1 fpNyh

6. gpuz;il -1 fpNyh

7. gRQ;rhzk; -5fpNyh

8. gRq;Nfhkpak; -10 ypl;lH

9. rzy; Nfhzpg;ig - 2

10. nty;yk; -1fpNyh

jahhpf;Fk; Kiw:-

NkNy Fwpg;gpl;l jiofis ed;whf ,bj;J

nfhs;sNtz;Lk;. ,ij XH rzy;

Nfhzpg;igapy; %l;ilahf fl;bnfhs;sTk;.



gRQ;rhzj;ij kw;nwhU Nfhzpapy; %l;ilahf

fl;b nfhs;sNtz;Lk;. 90 ypl;lH jz;zPH> 10

ypl;lH Nfhkpak;> 1 fpNyh nty;yj;ij ,bj;J

gpsh];bf; buk;kpy; NrHf;fTk;. gpwF ,uz;L

Nfhzp %l;ilfisAk; buk;kpy; %o;fp

,Uf;FkhW ghHj;J nfhs;sNtz;Lk;. ehs;NjhWk;

Nfhzp %l;ilfis Ml;btplNtz;Lk;. ,jpy;

cs;s rhuk; jz;zPhpy; fiuAk;. Gjpide;J

ehl;fs; ,g;gb njhlHe;J nra;aTk;.

gad;gLj;Jk; Kiw:-

gj;J ypl;lH jz;zPhpy; xU ypl;lH jhtu G+r;rp

tpul;bia fye;J njspg;ghd; nfhz;L fhiy

my;yJ khiyNtisapy; njspf;fTk;.

gad;fs;:-

,ij gapHfs; Nky; njspf;Fk; NghJ jhtu

G+r;rptpul;bahfTk;> gapH tsHr;rp Cf;fpahfTk;



gad;gLfpwJ. 25 ehl;fs; ,ilntspapy; ,uz;L

my;yJ %d;W Kiw njspf;fTk;.

----------------------------------------------------------------

5. japH %ypif fiury;

NjitahdnghUl;fs;:-

1. Ntg;gq;nfhl;ilj;J}s; -1 fpNyh

2. gRe;japH -2ypl;lH

3. gRq;Nfhkpak; -3ypl;lH

4. fLf;fha; J}s; -25 fpuhk;

5. mjpkJuk; J}s; -25 fpuhk;

6. ngUq;fha J}s; -25 fpuhk;

7. jz;zPH -5 ypl;lH

jahhpf;Fk; Kiw:-

NkNy Fwpg;gpl;l Ntg;gq;nfhl;ilj;J}is

xUJzpapy; itj;Jfl;b 5 ypl;lHjz;zPhpy; 24

kzpNeuk; itj;jpUe;J gpwF ed;whf gpope;J



tbfl;bnfhs;sTk;. tbfl;ba Ntk;GfiurYld;

gRkhl;LNfhkpak;> gRe;japH> fLf;fha; J}s;>

mjpkJuk; J}s;> ngUq;fha J}s; Mfpatw;iw

NrHj;J ed;whf fye;Jnfhs;sTk;. VO ehl;fs;

%b itf;fTk;.

gad;gLj;Jk; Kiw:-

xU ypl;lH fiurypy; gj;Jypl;lH jz;zPH fye;J

gapHfs; G+f;Fk; jUzj;jpy; fhiy my;yJ

khiyNtisapy; njspf;fTk;. Fwpg;ghf fha;fwp

nrbfs;> G+Q;nrbfSf;F ey;y gad; jUk;. ,e;j

%ypif fiury; G+ nkhl;Lf;fis G+f;f

J}z;btpLk;.



6. khl;L nfhk;G cuk;

Njitahd nghUl;fs;:

fwit khl;bd; rhzk;

fwit khl;L nfhk;G

Njq;fha; ehHf;fopTfs;

jahhpf;Fk; Kiw:

gRkhl;L nfhk;gpid vLj;J mjDs;

Njitahd msT fwit khl;bd; rhzj;ij

mOj;jkhf epug;gTk;.

nfhk;gpd; tha;g;Gwj;ij Njq;fha; ehH

fopTfshy; fhw;WGfhjthW epug;gTk;.

khl;L nfhk;ig Gijf;Fk; Kiw:

40 Kjy; 60 nr.kP msT MoKs;s Fopia

Njhz;lTk;.



gpd;G Kd;dH epug;gp itj;j khl;Lf;nfhk;gpd;

tha;g;Gwk; G+kpia Nehf;fp ,Uf;FkhW

Gijf;fTk;.

%d;W Kjy; ehd;F khjq;fSf;F gpwF

Fopapid Njhz;b Gijj;J itj;j khl;L

nfhk;Gfis Nrfupf;fTk;.

gad;gLj;Jk; Kiw:

25fpuhk; cuj;ij 13 ypl;lH jz;zPhpy; ed;whf

fyf;fp fhiy my;yJ khiy Ntisapy;

gapHfSf;F njspf;fyhk;.

gad;fs;:

,J gapH tsHr;rpA+f;fpahfTk;> kz;

tsj;ijAk;> kz;GOtpd; nray;jpwid

Nkk;gLj;JfpwJ.

----------------------------------------------------------------



7. %ypif rhz cuk;

Njitahd nghUl;fs;:

khl;L rhzk; -60fpNyh

Kl;il XL -200fpuhk;

Mo;Jis fpzw;W kz; (NghHnty; kz;) -

300fpuhk;

jahhpf;Fk; Kiw:

khl;L rhzk;> Kl;il XL> fpzw;W kz;

Mfpatw;iw ed;whf fyf;fTk;.

Fop vLj;jy;:

xU kPl;lH mfyk; miu kPl;lH ePsk; kw;Wk;

45- 90nr.kP Mok; nfhz;l Fopapid

Njhz;lTk;.

mjpy; nrq;fw;fis Fopapd; ntspg;Gwj;jpy;

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Particulars

S.No. Content Pg.

No

1 STUDIES ON ETHANOL PRODUCTION

FROM SPOILED FRUITS BY BATCH

FERMENTATIONS

S.KARUNAKARAN, ARCHANA.R and

KOMATHISREE P.S

1

2 STUDIES ON ETHANOL PRODUCTION


FERMENTATIONS

S.KARUNAKARAN, ARCHANA.R, and

KOMATHISREE P.S

3

3 PRODUCTION OF VERMICOMPOST

AND VERMIWASH

SWATHI.A

5

4 ISOLATION AND

CHARACTERIZATION OF

ORGANOPHOSPHOROUS DEGRADING

BACTERIUM ISOLATED FROM

AGRICULTURAL SOIL

STEPHY MARY.A, PRIYA.T AND

MS.J.ESTHER HELLAN PRASANNA

8

5 SYNTHESIS AND

CHARECTERIZATION OF SILICA

10

NANOPARTICLES FOR DRUG

DELIVERY IN CANCER TREATMENT

S. DHANASEKARAN, S.

KARUNAKARAN, P. JAYANTHI AND P.

PRATHEEPA

6 STUDIES ON PRODUCTION OF

LACCASE FROM Pleurotus sp.

S.SUSHMITHA, S.MUSILIN WINMIKCA

AND PENNARASI

13

7 EFFECT OF AZOLLA AND SEA WEED

EXTRACT ON GERMINATION OF

VARIOUS CROPS – A COMPARATIVE

STUDY

R. Sripriya, S. Nancy Roy and

P.K.Gayathri

15

Nat. Appl. Green Tech. Impro. Agro in India – A Biotech. Approach


STUDIES ON ETHANOL PRODUCTION


FERMENTATIONS

S.KARUNAKARAN, ARCHANA.R and

KOMATHISREE P.S.


Vivekanandha College of Engineering for Women

Elayampalayam, Tiruchengodu

ABSTRACT:-

India is the second major producer of fruits and

vegetables. In the world, particularly grapeOrange, apple,

pineapple, banana, and papaya (Carica Papaya) some

microorganisms isolatedfrom the peels, pulp and juice,

ash, pH, moisture, lipid, especially Acetobacter aceti,

Clostridium butyrium, Bacillus sp and Micrococcus, some

fungal microbes. Aspergillus flavus, A.niger,penicillium,



chrysogenum, and Fusarium species were best

fermentation process and gives thenutritive values. The

maximum amount of nutritive compounds were present in

papaya, fruitsthere composition of papaya was moisture

content 8.5 to 93.1 calcium, Phosphorus 6.8 to

8.9%,Protein 0.47 to 2.15 and fat 0.04 to 0.5%

respectively. Papaya Juice had the best organileinicquality

however the most ethanol distillate was produced from

Papaya. The papaya fruit pulpfrom raw starch Hydrolate

are attractive resources for economical production of

ethanol.

Key words: Papaya fruits, sacharification, Alcoholic

fermentation, Distillation.



STUDIES ON ETHANOL PRODUCTION


FERMENTATIONS

S.KARUNAKARAN, ARCHANA.R, and

KOMATHISREE P.S.




ABSTRACT:-

India is the second major producer of fruits and

vegetables. In the world, particularly grape Orange, apple,

pineapple, banana, and papaya (Carica Papaya) some

microorganisms isolated from the peels, pulp and juice,

ash, pH, moisture, lipid, especially Acetobacter aceti,

Clostridium butyrium, Bacillussp and Micrococcus, some



fungal microbes. Aspergillus flavus, A.niger, penicillium,

chrysogenum, and Fusarium species were best

fermentation process and gives the nutritive values. The

maximum amount of nutritive compounds were present in

papaya, fruits there composition of papaya was moisture

content 8.5 to 93.1 calcium, Phosphorus 6.8 to 8.9%,

Protein 0.47 to 2.15 and fat 0.04 to 0.5% respectively.

Papaya Juice had the best organileinic quality however

the most ethanol distillate was produced from Papaya.

The papaya fruit pulp from raw starch Hydrolate are

attractive resources for economical production of ethanol.

Key words: Papaya fruits, sacharification, Alcoholic

fermentation, Distillation.



PRODUCTION OF VERMICOMPOST AND

VERMIWASH

SWATHI. A

Dept of Biotechnology,

Rajalakshmi Engineering College

Thandalam, Chennai

[email protected]

Abstract

Waste management is one of the major issues faced

all around the world.Vermicompost is emerging as

“environmentally sustainable” “economically viable” and

“socially acceptable” technology all over the world in

addressing this issue. Vermicomposting is performed to

convert domestic farmland waste into manure which has a

good NPK content and suitable for use. Vermicomposting

is a process in which earthworms are employed for

biodegradation of organic waste. Earthworms feed on

mailto:[email protected]



organic waste and its excreta enhances the nutrient

content of the manure. Special bins are used for

vermicomposting in order to collect Vermiwash which is

a potent liquid bio fertilizer. Also the bins are portable

and easy to handle hence providing a much advantageous

production method. In this process earthworms along with

alittle amount of soil and pre-degraded wastes were

layered in the bin. The whole process of degradation was

carefully monitored, keeping in check, all the key

parameters like predators for one month. The bio manure

along with the Vermiwash was collected regularly after

10 days. Vermicompost was collected at the end of this

period. The Vermiwash and Vermicompost was

experimented and the various results have been

documented including NPK content which was obtained

as 0.013%, 1.715%, 24 ppm and 1.25%, 0.19%,

0.28%respectively. The study shows various techniques



and difficulties faced during the process which resulted in

narrowing down to few efficient ones. The field

experiment studied on the weekly basis by implementing

this production of Vermicompost and Vermiwash proved

satisfactory, the results of which along with the avenues

for future development in it is also discussed in this paper.

KEY WORDS:

Vermicompost, Vermiwash, bins, nutrient value.



ISOLATION AND CHARACTERIZATION OF

ORGANOPHOSPHOROUS DEGRADING

BACTERIUM ISOLATED FROM

AGRICULTURAL SOIL

Stephy Mary.A, Priya.T and Ms.J.Esther Hellan

Prasanna


Vivekanandha College of Engineering for Women,


ABSTRACT

Organophosphorous pesticides are the world’s most

widely used pesticides in agriculture. Exposure of these

pesticides and its metabolites have been related to a

variety of nerve disorders in humans. Microbial

degradation is considered to be a cost effective method

for decontamination of toxic organophosphorous

pesticides from the environment. In this study we isolated



the bacteria from different agricultural field soil to

degrade the Organophosphorous pesticides

(Monocrotophos, Dichlorovos, chloropyrifos, diozinon)

as a soul carbon source. And the results of biochemical

tests shows that Bacillus sp. Proteus sp. serratia

sp.effectively degrade the organophosphorus pesticides.

16s rRNA Gene sequencing results show that Bacillus sp

is responsible for degrading MCP Pesticide than others.

Ussing thin layer chromatography technique find out the

RF values that confirms the genus. It is evident from the

results that the isolated Bacillus sp. could be used for

bioremediate the area contaminated with

organophosphorous pesticides. Thus we can remediate the

environmental pollution.



SYNTHESIS AND CHARECTERIZATION OF

SILICA NANOPARTICLES FOR DRUG

DELIVERY IN CANCER TREATMENT S. Dhanasekaran, S. Karunakaran, P. Jayanthi and P.

Pratheepa




ABSTRACT

Cancer is a potentially fatal disease caused mainly by

environmental factors that mutate genes encoding critical

cell-regulatory proteins Lung cancer is one of the most

common types of cancer and cases in increasing yearly

wordwide. The resultant aberrant cell behavior leads to

expansive masses of abnormal cells that destroy

surrounding normal tissue and can spread to vital organs

resulting in disseminated disease, commonly a harbinger

of imminent patient death. Cancer is a complex genetic



disease that is caused primarily by environmental factors.

The cancer-causing agents (carcinogens) can be present in

food and water, in the air,

and in chemicals and sunlight that people are exposed .

Nanoparticle drug delivery is one area of recent

research significance since incorporation of drug to nano

particles enhances therapeutic effectiveness and reduces

side effects of the drug payloads improving their

pharmacokinetics. In the present study Mesoporous silica

nanoparticles were synthesized, loaded with anticancer

drug 5FU and characterized by Zeta analysis to have a

average size of 288nm. The drug release studies shows

sustained release of drug. In vitro anticancer activity on

lung carcinoma cells shows increased cell death in a dose

dependent manner. Induction of apoptosis was evident

from fluorescent staining. From the results it can be

suggested that Mesoporous silica nanoparticles loaded



with 5 FU can be considered as a potent candidate for

chemotherapy.



Studies on production of laccase from

Pleurotus sp. S.Sushmitha, S.Musilin Winmikca and Pennarasi



Tiruchengodu

ABSTRACT

Lignin is the most abundant aromatic substance

present in biosphere mainly secondary cell walls of plants

and few algae.It is a non-carbohydrate aromatic

heteropolymer composed of phenylpropanoid units linked

through a variety of non hydrolysable C-C and C-O-C

bonds.Research on lignin is important for conversion of

solid organic waste into production of valuable

compounds like biofuel and maintenance of global carbon

cycling in the field of environmental engineering.This

recalcitrant organic compound has been effectively

degraded by microbes like fungi,actinomycetes and

bacteria through oxidation process.Laccasses are copper



containing lignin degrading enzymes often extracellular in

nature.They use molecular oxygen to oxidize a wide

range of aromatic compounds. A Pleurotus sp showed

positive for laccase, lignin peroxidase and manganese

peroxidase production. The growth of the test strain and

laccase production was high in lignin degrading enzymes.

Numerous substrates are used for enzyme activity.

Laccase isozymes have been purified 32.4-fold from the

crude enzyme protein through anion exchange

chromatography, preparative gel electrophoresis, and

electro elution. Their estimated molecular weights are

3.5–43 kDa. Pleurotus sp has potential applications for the

cleanup of industrial effluents in paper and pulp industry.



EFFECT OF AZOLLA AND SEA WEED

EXTRACT ON GERMINATION OF

VARIOUS CROPS – A COMPARATIVE

STUDY

R. Sripriya, S. Nancy Roy and P.K.Gayathri

Dept of Biotechnology,

Vel Tech High Tech Dr. Rangarajan Dr. Sakunthala

Engg. College, Chennai - 62

ABSTRACT

A comparative study of biofertilizers (Azolla and

seaweed) was carried out. Azolla (Azollapinnata) and

Seaweed (Enteromorphaintestinalis) contain

considerable amount of micronutrients and some plant

growth hormones which helps plant growth and also in

germination. Seeds of Abelmoschusesculentus,

Solanumlycopersicum and Vignaunguiculata were



treated withAzolla liquid fertilizer extracted from dried

Azolla. The result showed that20% concentration of

aqueous extract of Azolla promoted better germination

and growth. The above mentioned procedure was also

followed for seaweed extract . We analyzed nearly the

same germination rate for seaweed extract in

comparison with Azolla extract. However, the plant

growth observed in seaweed extract waslow. From this

experiment, we came to know that seaweed can also be

used as an alternate for the development of crops.

Keywords: Azolla, Seaweed, Biofertilizer,

Germination.

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