xn-series and the reason(s) for selection the new capabilities of plt … · manual plt estimate...

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© 2015 Sysmex America, Inc. All rights reserved.

XN-Series Technology

“What’s Inside the Box?”

© 2015 Sysmex America, Inc. All rights reserved.© 2015 Sysmex America, Inc. All rights reserved.

OBJECTIVES

Describe the technologies available on the XN-Series and the reason(s) for selection

Utilize example case studies to illustrate the new capabilities of PLT-F technology used on the XN-Series

Describe the advanced reagent technology of the RU-20


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BEYOND TECHNOLOGY BEYOND EXPECTATIONS

Quality

Dependability

Accuracy


greater accuracy, specificity, productivity

NEXT GENERATION

DIAGNOSTICSsuperior insight and control, enhancing speed to treatment

ADVANCED TOOLS & TECHNOLOGIES

automatically balances work-flow and routine tasks

PROCESS OPTIMIZATION

driving improvement,supporting your staff,

helping you plan for the future

HARMONIZED SUPPORT


greater accuracy, specificity, productivity

NEXT GENERATION

DIAGNOSTICSsuperior insight and control, enhancing speed to treatment

ADVANCED TOOLS & TECHNOLOGIES

automatically balances work-flow and routine tasks

PROCESS OPTIMIZATION

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N E X T G E N E R AT I O N D I A G N O S T I C S

THE MOST ADVANCED HEMATOLOGY DIAGNOSTICS ON THE MARKET

Fluorescent Platelet

Rerun/Reflex Testing

Body Fluid Differentials

Advanced Clinical

Parameters


XN-SERIES

Same Analytical Module

XN-1000 XN-2000XN-3000 XN-9000

Up to 100 /hr /module

Common Reagent System

Common QC System

Common Software System

XN-1000

SA-01

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SINGLE MODULE – FULLY LOADED

Every XN Configuration can include:

All the parameters

All the features

All the capabilities


P R O C E S S O P T I M I Z AT I O N

EXCEPTIONS TO THE RULE: DIFFERENT XN-1000 SAMPLERS

Simple SamplerSA-01

Reflex SamplerSA-10



DIFFERENT XN-1000 SAMPLERS

Simple SamplerSA‐01

Reflex SamplerSA‐10

Smart Start

Reflex/Rerun capabilities

Rack bar code read capability

MAP tubes in rack mode

MAP tubes in manual mode

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XN WITH SIMPLE SAMPLER OPTIONS


XN-Series Technology

Analytical Technologies


SYSMEX XE VS. XN TECHNOLOGY

RBC, PLT, Hgb

WBC / BASO

NRBC DIFFRET / PLT‐O

RBC, PLT, Hgb

WNR WDF RET PLT‐F

NEW NEW NEW


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SYSMEX FOUNDATIONAL TECHNOLOGY

RBC & PlateletDC Detection / Hydrodynamic Focusing

HemoglobinAbsorbance / Sulfolyser



FLUORESCENT FLOW CYTOMETRY

WNR channel WDF channel PLT-F channel RET channel


Side Fluorescent LightDNA/RNA information

Side Scattered LightCell inside structure information

Forward Scattered LightCell size information

Dichroic Mirror

Laser Beam wavelength=633nm



WNR CHANNEL

BASO LYMPH MONO Granulocyte

NRBC RBC

Polymethine Dye

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WNR CHANNEL

NRBC

WBC

BASO

Debris

SFL

FS

C NRBCs with EVERY CBC



ADVANTAGE OF FLUOROCELL WNR

SFL

FS

C

SFL



NRBC CORRELATION DATA

Correlation Coefficient (R) = 0.9179 N = 171

Correlation Coefficient (R) = 0.9219 N = 171

Data from the University of Iowa / Dec 2011

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MEGAKARYOCYTES



MEGAKARYOCYTES



WNR CHANNEL

Maximized efficiencyNRBC with every CBC

No additional steps or reagents

Accurate WBC counts in the presence of NRBCs

Virtually eliminates interferences from

Lyse resistant RBCs

Lipids

Megakaryocytes

Improves turn around times

BASO

LYMPH

MONO

Granulocyte

NRBC

RBCDebris

FS

C

SFL

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WDF CHANNEL

LYMPH MONO NEUT EO

Atypical LYMPH Immature WBC

Polymethine Dye



WDF CHANNEL

LYMPH NEUT+BASO

EO

MONO

Debris

IG

SF

L

SSC



IG – XN DIFF ABSTRACT

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ENHANCED FLAGGING ALGORITHMS

(Sysmex Adaptive Flagging Algorithm

based on Shape-recognition)

LDA (Linear Discriminant Analysis)

Detects abnormal cells - (with high sensitivity)

SAFLAS method



Normal Abnormal (Blasts?) Abnormal (Abn Lympho?)

WDF SAFLAS METHOD



LOW WBC ANALYSIS MODE

WB (Whole Blood) mode LW (Low WBC) mode

Dilution Rate :Analysis Volume :Whole Counts *2 :

Dilution Rate :Analysis Volume :Whole Counts *2 :

x 61.158.2µL9,530

x 61.1174.6µL28,600

Analysis Time extended to 3 times the Whole Blood WBC count time

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LOW WBC ANALYSIS MODE

Analysis time extended to 3 times WB WBC count time

Better accuracy and precision on counts < 0.50 x 103

No Vote Outs – Differential results on all counts

Increased reportable differentials



WDF CHANNEL

Improved efficiency More reportable automated differentials

6-part differential with reportable IG%

Rapid TAT

Decreased manual interventions

Enhanced Flagging

Auto-correction of lymphswhen NRBC are presentLYMPH

MONO

NEUT

EO

Atypical LYMPH

Immature WBC

SF

L

SSC



BODY FLUID ANALYSIS

Cerebrospinal fluid (CSF)

Body fluid mode (target species)

Pleural fluid

Peritoneal fluid

Synovial fluid

HF-BF

MN

PMN

MO-BF

LY-BF

EO-BFNE-BF

WDF Scattergram

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BODY FLUID ANALYSIS

No pre-analysis preparation

No additional reagents

Automated background counts

Rapid analysis



PLT-F CHANNEL

RBC PLT IPF

Oxazine Dye



WHY A SECOND PLATELET CHANNEL?

Impedance platelet analysis (size) has limitations

Identification and discrimination of platelets from interfering particles with the same size

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False Increases

False Decreases

BacteriaImmune complexes

WBC fragments

RBC fragments

Microcytes

Giant platelets

Platelet clumps

INTERFERENCES INIMPEDANCE PLATELET COUNTING



PLT-F CHANNEL

RBC

PLT-F

IPF

FS

C

SFL



GIANT PLATELETS & PLT-F

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DxH 800 Platelet 114Manual plt estimate 200


DxH 800 Platelet 114Manual plt estimate 200



PLT-F CHANNEL

Second method of platelet

Fluorescent Dye specific for platelet organelleExtended count time (6 times) for accurate platelet enumeration, especially in low platelet counts

Good comparison with CD41/CD61

Minimizes interference from RBC fragments, microcytic RBC’s and WBC fragments

Automated reflex with on board rules“BPR systems” only

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RET CHANNEL

RET RBCWBC PLT

Polymethine Dye



RET CHANNEL

PLT

RET

RBC



RETICULOCYTE PARAMETERS

• Reticulocytes – # and % of immature RBC’s

• Immature Reticulocyte Fraction– Newly released from the

marrow, a direct cellular measurement of erythropoiesis

• Reticulocyte Hemoglobin– Direct cellular measure of iron

availability

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RETICULOCYTE CHANNEL

Clinical UtilityMonitor RBC development at the cellular level

IRF for Retic production

RET-He for effectiveness of hematopoiesis

Clinically relevant information for the management of anemia in conjunction with other available clinical information

Operational EfficiencyReduced Interference From:

WBCs

Howell Jolley Bodies

Parasites

Sickle cells

Quick and automatic



XN TECHNOLOGY SUMMARY

WNR ChannelMaximized efficiency

NRBC the first time – all the time

Accurate WBC Counts in the presence of NRBCs

WDF ChannelEnhanced Flagging

6-part differential (including IG)

Improved Sensitivity and Specificity



XN TECHNOLOGY SUMMARY

Low WBCBetter accuracy and precision on counts < 0.50 x 103

No Vote Outs – Differential results on all counts

Increased Reportable differentials

PLT-FFluorescent Dye specific for platelet organelle

Extended count time (6 times) for accurate low platelet enumeration

Good comparison with CD41/CD61

Automated reflex with on board rules

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XN Technology

Reagents – RU-20


A D VA N C E D T O O L S & T E C H N O L O G I E S

SMART REAGENT MANAGEMENT SYSTEM

RU-20 Reagent Management System25x concentrate

Reduces reagent changes and instrument idle time

Increases free space in the lab.

CELLPACK DCL compared with RU-20 and CELLPACK DST



WHY IT MATTERS……

1 cube of diluent weighs ~48lbsAt 300 samples/day you move about 156 cubes/year

RU-20 = 1 cube/50 days~7.2 cubes/year

Model that over 5 years With an average of 3 physical moves of each cube

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Why it matters……


XN Technology

Automation & Process Optimization




THE SYSMEX® XN-SERIES brings greater accuracy and efficiency throughout the entire testing chain

S Y S M E X X N - S E R I E S

X N - 1 0 0 0 ™X N - 2 0 0 0 ™

X N - 3 0 0 0 ™

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XN-SERIES ON-BOARD RULES

Enhanced Walk-Away CapabilitiesAutomated Reflex or Rerun testing

No Need To Search For Tube



CO-PRIMARY SYSTEM

Fully Automated Workload Balancing

Automatic re-run/reflex testing

XN-2000 and XN-3000



AUTOMATED SLIDE MAKING

4th generation system

Fully integrated

Fully reflexive slide preparation

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SLIDEMAKER / STAINER

Improves smear TATRules to define specific slide types

Non-batch staining

Consistent smear quality

Re-cycling reagent system

Provides positive patient ID:3 slide print options available

Low sample volume requirements:

Micro-sample port

Blood aspirated and slide made when indicated




THE SYSMEX® XN-SERIES brings greater accuracy and efficiency throughout the entire testing chain

S Y S M E X X N - S E R I E S

X N - 9 0 0 0 ™


Now You Know What’s Inside the Box

Thank You

xn-series and the reason(s) for selection the new capabilities of plt … · manual plt estimate...

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