Contents

SPECIAL LECTURES

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Integration of the Tools of Mendelian and Molecular Genetics in Crop ImprovementM. S. SwaminathanIn-vitro Induced Haploids in Higher PlantsBu BanGenetic Manipulation in Crops: A Symposium ReviewW. R. Scowcroft

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I. HAPLOID

1 New Strategies for Pollen Plant Formation in vitroE. Heberle-Bors

2 Induction of Pollen Plant Formation in Barley Anther CultureK. N. Kao and D. C. Horn

3 Haploid Production in Japanese Wheat Cultivars Crossed with Hordeum bulbosumM. N. lnagaki and M. Kamio

4 Anther and Ovary Culture of Helianthus annuus L.G. Mix

5 Haploid Plant Induction in Apple (Malus prunifolia) and Cytologicaland Histological StudiesWu Jiangyun, Huang Dingqiu and HanDongsheng

6 Inheritance in Rice Pollen Plants and Its Application in Rice ImprovementChen Ying and Li Liang-cai

7 The Application of Pollen Sporophytes of Winter Wheat in Breeding -theDevelopment of the New Variety 'Jinghua No.1'Hu Daofen, Yuan Chendung, Li Hongchao and Liu Jianping

8 Distinguishing Features of Anther Culture in Woody PlantsChen Zhenghua

9 Aneuploids Produced from the Progeny of Anther-cultured WheatPollen Plants (Triticum aestivum)Zhang Yu Ling, Li De Shen and Pan Wei Hua

10 Genetic Aspects of Induction and Use of Haploids fromWheat (Triticum aestivum) and Spelta (T. spelta)

11 Breeding; Evaluation and Utilization of Anther-cultured Varieties'Xin Xiou' and 'Hua Han Zao' in Rice (Oryza sativa L.)Zhang Zhenghua, Zheng Zhuling, Gao Yanhua and Cao Huaxin

12 Effects of Gamma Radiation on Anther Culture of RiceYin Dao-chuan, Wei Qi-jian, Yu Qiu-cheng and Wang Lu

13 Effects of Gamma Radiation on Wheat Pollen Development in Anther CultureVin Dan-chuan. Wei Oi-iian. Vu Oiu-chenl! and Wanl! Lu

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14 Application of Anther Culture to 'Convergent Improvement' in Rice BreedingLi Mei-fang, Ni Pi-chong and Shen Iin-hua

15 Genetic Improvement of Fruit Crops Through Tissue CultureA. F. Mascarenhas, S. Nair, R. S. 1yer and P. K. Gupta

16 Character Inheritance and Application of Pollen Plants Derived fromHybrid Rice (Oryza sativa subsp. Hsien)Zhu De-yao, Pan Xi-gan and Chen Cheng-yao

17 DNA Microphotometry of Hordeum vulgare Pollen During Cold PretreatmentXu Zhi-hong and N. Sunderland

18 Differentiation in Anther Culture of Two CucurbitsH. Kumar

19 Embryogenesis in Anther Cultures of MaizeKuo Chung-shen and Lu Wen-liang

20 Pollen Division in Early Stages of Anther Culture in WheatSun Ching-san, Chu Chih-ching and Li Shou-quan

21 Ultrastructural Features of Pollen Embryogenesis in Barley, Wheat and RiceHuang Bin

22 Biochemical Cytology of Pollen EmbryogenesisV. Raghavan

23 Influence of Genotype, Plant Growth Temperature and Anther IncubationTemperature on Microspore Embryo Production in Brassica napus ssp. oleiferaI. M. Dunwell, M. Cornish and A. G. L. De Courcel

24 Anther Culture in Cowpea (Vigna unguiculata L. Walp)T. A. 0. Ladeinde

25 1n-vitro Gynogenesis in Unfertilized Ovaries and OvulesYang Hong yuan, Zhou Chang, Tian Huigiao, Liu Zhonglai, Huang Qunfei,Gai Detian and Yan Hua

26 Genetic Analysis of the High Yield of Hybrid Rape of Gynogenetic Haploid OriginLuo Peng, Liang Minsan, Huang Min and Wu Suhui

27 Cytogenetics of Aneuploids Derived from Pollen Plants in Rice ( Oryza sativa L. )Chu Qiren and Zhang Lingnan

28 Chromosomal Variation in the Pollen Grains of a CultivatedVariety of Colocasia antiquorumG. N. Bhattacharya

29 The Cytological Characters of the Pollen Plants from Hybrid Flof 6x Triticale and Common WheatWang Xingzhi and Hu Han

30 Variation of Pollen-derived Plants in Wheat (Triticum aestivum)Z. Y. Xi, I. K. ling and Hu Han

31 Quantitative Inheritance of Differences Between Self-pollinated Androgenetic andGynogenetic Lines in Rice and BarleyY. Dattee, H. San, 1. Andre, A. Odjo and Y. Kouadio

32 Cytological Observations on Calluses and Cell Clones Originatingfrom Maize Pollen and Their Regenerated PlantsGu Mingguang

33 Characters of Regenerate Plants from Maize Haploid and DiploidEmbryonic Cells and Mutants of Stomatal ComplexesCao Ziyi, Leng Guanti and Guo Caiyue

34 Callus Initiation and Regeneration and Selection of Somaclones in RiceGo M. Reddy and P. B. K. Kishor

35 Induction and Genetic Expression of Haploid Poplar PlantsWu Kexian and Xu Miaozhen

36 Study on Pollen Plants and Their Clones in HeveaXu Xuen, Zhang Shijie, Ou Xiaohui, Pan~ Renshen~ and Chen Zhenf!hua

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37 Ploidy Variations in Plants of Datura innoxia Mill. , Arising from ExperimentalAndrogenesis or Somatic EmbryogenesisR. S. Sangwan, B. S. Sangwan-Norreel and G. Sergheraert

38 Genotypic Variation in Anther Culture of WheatI. W. Ouyang, S.E. Iia, S. M. Zhou, D. G. He and G. H. Feng

39 Triticale Anther CultureG. Schumann, E. R. I. Keller, R. Franke and H. Leike

40 In-vitro Gynogenesis in Some Cultivated SpeciesL. H. San, P. Gelebart, P. C;h. Hamelin, D. Hosemans,D. Bossoutrot and I. Andre

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II. MUTATION, MUTAGENESIS

1 Induced Variation in NaCI Tolerance with EMS in Trifoliate Orange in vitroH. Yamaguchi and K. Matsumoto

2 The Expression of M3 Plants of NaCI-tolerant Pollen Plants in RiceChen Shaolin, Tian Wenzhong and Zhang Guihua

3 Effect of Repeated Selection in vitro on Amino Acid Content in MaizeChen Shaolin, Zhang Guihua and Tian Wenzhong

4 A Study of the Nature, Magnitude and Frequency of MicromutationsInduced in Homozygous and Heterozygous Genetic Backgrounds in

Cowpea (Vigna unguiculata)D. M. Mahishi and G. Shivashankar

5 In-vitro Selection for Resistance to Mineral StressC. P. Meredith and A. I. Conner

6 Selection for Tolerance of Thiocarbamate Herbicides via Cell CultureS. M. Flashman

7 Selection of Wheat Resistant to Glume Blotch Disease Through Tissue CultureR. Brettell, A. Brown and B. Whan

8 The Use of Potato Protoplasts to Study Virus ResistanceM. I. Foxe and I. Prakash

9 Biochemical Characteristics of Nitrate Reductase Deficient MutantsChang Teh- Yi, Cheng Wei, Lu Iia-Ling, Yu Ling-Feng, He Wen-Zhuand Tang Yu-Wei

10 Induced Mutation in Brewing BarleyE. lanko

11 Manipulation of Gene Actions Through Other Genes and Environmental FactorsW. Gottschalk

12 Transcription and Translation Processes in Sugar-cane Cells Affected by Ametryn0. I. Crocomo, N. Ochoa-Alejo and E. T. Oliveira

13 New Achievement in Research on Soya-bean Mutations Induced by Gamma, RaysS. Sumtkupt, A. Wongpiyasatid and S. Lamseejan

14 Use of Phytotoxin and Tissue Culture in Evolving Disease-resistant Rice

GermplasmP. Vidhyasekaran, E. Borromeo, v. H. Ling and T. W. Mew

15 Study on the Cell Selection of Mutants Resistant to Phytophthoraparasitica var. nicotianaeZhou Iiaping, Huang He and Pang Lung

16 A Norleucine-resistant Mutant Cell Line from Haploid Nicotiana tabacum L.He Zhuopei (Ho Chue-pui), Xu Zhuyun, Xu Shuping and Loo Shihwei

17 Effects of Pingyangmycjn and Bleomycin As on the Inhibition of DNAReplication in Barley ChromosomesH. Yamaguchi, A. Tatara and Gu Aiqiu

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18 Streptomycin-resistant and Abnormally Flowered Mutants of TobaccoChen Jiayu, Xu Jinxiang, Wang Peitian, Zhao Shimin and Wang Lianqing

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III. SOMACLONAL V ARIA TION

1 Ribulose Bisphosphate Carboxylase Activity in Anther-derived Plants of AfricanViolet. .

R. H. Smith and S. Bhaskaran2 Somaclonal Variation and Wheat Improvement

P. Larkin, S. Ryan, R. Brettell and w. Scowcroft3 Somaclonal Variation and Rice Improvement

Zhao Cheng-zhang, Zheng Kang-le, Sun Zong-xiu and Qi Xiu-fang4 Somaclonal Variation and Genetic Manipulation of Potato

S. Bright, G. Ooms, A. Karp, D. Foulger, N. Fish, M. Jones and N. Evans5 Somaclonal Variation in Spike Characteristics of Wheat

B. B. Johnson and H. Nguyen6 Tissue Culture of US Rice Varieties: Development of Short-Stature Variants

T. P. Croughan7 Tissue Culture of Lily and Other Ornamentals

Huang li-ming8 Induction of Endosperm Plantlets of' Jinfeng' Pear in vitro and Their Ploidy

Zhao Huixiang9 Biotechnology of the Conservation of Germplasm and Its Implications in

Agriculture and ForestryY. P. S. Bajaj

10 Morphological and Chromosomal Changes of Regenerated Plants fromInflorescence Calluses of Hordeum vulgare X Triticum aestivum HybridChu Chih-ching, Sun Ching-san, Chen Xiao, Zhang Wen-xiang and Du Zhen-hua

11 Application of the Blue-grained Monosomic to Wheat BreedingLi Zhensheng, Mu Sumei, Zhou Hanping and Wu Iingke

12 Cool Temperature Seedling Vigour of R2 and M2 Short-Stature Rice (Oryzasativa L.) SelectionsK. S. McKenzie and T. P. Croughan

13 Variability Induced in Nicotiana sylvestris by Two Successive Cycles ofProtoplast CultureX. Q. Li, D. Prat, R. de Paepe and I. Pernes

14 Characteristics and Chromosomal Variation of Somaclones and Their Progeny inRice (Oryza sativa L.)Zhang Lingnan and Chu Qiren

15 Salt.tolerant Flax Plants Regenerated from Cells Surviving a Highly SalineCulture MediumA. McHughen

16 Somaclonal Variation in Some Agronomic and Physiological Characters of WheatS. Ryan

17 In-vitro Induction of Callus and Regeneration of Whole Plants from HypocotylExplants of Cowpea (Vigna unguiculata)A. N. S. Gowda and B. A. Satyan

18 Genotypic Differences in Callus Induction in the Genus MacrotylomaP. K. Ananda Rao, A. N. S. Gowda and B. A. Satyan

19 Advances in Somaclonal VariationM. R. Sondahl, W. Sharp and D. Evans

20 Influence of the Male-Sterility Soya-Bean Gene (msJ on the FemaleGametophyte: Frequencies of Haploids and PolyploidsI. C. Kennell, L. F. Chen. H. T. Horner and R. G. Palmer

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21 Cytogenetic Stability of Cell Cultures from Plants of Androgenetic OriginB. Zeppernick and K.-H. Neumann

IV. SOMA TIC MORPHOGENESIS

1 Molecular Mechanisms of Somatic EmbryogenesisA. Komamine and K. Nomura

2 Regulation and Role of Glyoxalase-I in-in-viR'o Cultures of Higher PlantsS. K. Sopory, 0. Ramaswamy, S. Pal and S. G. Mukherjee

3 Embryogenesis and Endogenous Hormone Content of Cell Cultures of SomeCarrot Varieties (Daucus carota L.)Li Tianran and K.-H. Neumann

4 Effect of Specific Inhibitors and of Chromosomal Dicentrics on Development ofSomatic Carrot (Daucus carota) EmbryosV. Nuti Ronchi, M. A. Caligo, F. Toncelli, G. Luccarini, M. Nozzolini andG. Martini

5 Physiology of Shoot Initiation in Excised Radiata Pine CotyledonsCultured in vitroT. A. Thorpe and V. M. Villalobos

6 Embryogenesis from Sugar-cane ProtoplastsYan Qiusheng, Zhang Xueqin and Gu Mingguang

7 Control of Morphogenesis by Hormones and Phytochrome in Tissue CultureS. Guha-Mukherjee, N. Bhalla-Sarin, S. Bagga, K. Manoharan and S. K. Sopory

8 Single-Cell Cultivation and Plantlet RegenerationZhou Gaozhi, Ge Koulin, Yang Iinshui, Wang Yunchu, Ye Mingming andCai Yixin

9 Somatic Cell Culture and Plant Regeneration in Indica RiceD. S. Brar, D. H. Ling and F..I. Zapata

10 Autotetraploid Rice Clones Derived from the Elite Plants of Hybrid ProgeniesR. Z. Qin, D. Y. Wu, Z. Y. Chen, W. C. Song, Y. H. Zhangand W. K. Pao

11 Tissue Culture Studies on Some Crop LegumesK. K. Nag and I. P. Sinha

12 Induction of Embryoids and Plantlets from Oil Palm CallusCui Yuanfang, Gong Zheng, Chen Xinghua, Shao Xingdi and Chu Chihching

13 Regeneration of Multiple Shoots from Shoot-tip Culture of Vigna mungo(L.) HepperM. M. Haque, M. I. Hoque, A. Begum and A. S. Islam

14 Shoot-tip Culture of Black currant (Ribes nigrum L.) in vitro and Its Cytologicaland Histological ObservationHuang Dingqin, Wu Iiangyun and Zhou En

15 Plantlet Regeneration and in-vitro Flowering in Arachis hypogaea L.G. M. Reddy and S. B. Narasimhulu

16 Micropropagation and Bulb Morphogenesis of Narcissus in vitroKuo Chung-shen and Lu Wenliang

17 Rapid Multiplication in vitro of Asparagus racemosusD. K. Kar and S. Sen

18 Clonal Propagation of Seedless WatermelonGao Xin Yi, Wang Yu Ying and Sun Bai Ling

19 Somatic Cell Culture as a New Technique in Porphyra MaricultureTang Yanlin, Liu Wanshun, Dai Iixun and Fang Tsungci

20 Clonal Propagatihn of Barley Intergeneric Hybrids by Tissue CultureV. K. Shumny and L. A. Pershina

21 Embryogenesis and Plant Formation from Embryos of Cucumis metuliferusX C. anguriaG. Fa.f.fuliotLf and B. V. Nelson

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22 Recent Advances in Somatic Morphogenesis in Coconut (Cocos nucifera) andCardamom (Elettaria cardamomum)C, R, Raju, P. Prakash, K, B. Kumar and R. D. Iyer

23 Induction of Salt-tolerant Rice Callus and Regeneration of PlantsRan I lung and Song Rak Gun

24 Somatic Embryogenesis and Cell Culture in the GramineaeWang Dayuan

25 Biochemical and Genetic Analysi's of Somatic EmbryogenesisZ. R. Sung, F. LoSchiavo and A. Fienberg

26 In-vitro Clonal Propagation of Crop Plants in ChinaLi Wenan, Xu Zhihong and Loo Shihwei

27 Somatic Embryogf!.nesis and Plant Regeneration in IR Lines of RiceD. R. Ling, D. S. Brar and F, I. Zapata

28 Investigations on the Endogenous Hormonal System of Carrot Cell CulturesDuring EmbryogenesisLi Tianran, K.-R. Neumann, F. Schiifer and B, Stiebeling

V.PROTOPLASTS

I Application of Somatic Hybridization Technology to Plant BreedingW. A, Keller, R. S. Pandeya, S. C. Gleddie and G, Setterfield

2 Isolation and Fusion of Protoplasts from Spindle Tissues of Saccharumspontaneum, S. officinarum and Ripidium arundinaceumM. R. Sedano and L. M. Engle

3 Electrofusion and Micro-injection for the Introduction of Genetic Informationto Plant CellsY. Yamada, R, Morikawa and Y. Rayashi

4 Observation of Somatic Hybrid Progeny Between Tobacco and PetuniaRuang Meijuan, Li Wenbin, Sun Yongru, Zhang Wei and Li Xianghui

5 Asymmetrical Somatic Cell Hybrids Through Protoplast FusionF. Sala, C. Morandi, M. G. Biasini, C. Sala, E. Nielsen and B, Parisi

6 Genetic Manipulation of Cultivated Cells and Protoplasts of PotatoR. G. Butenko, L. M. Chromova, G. V. Rassadina, A.B. Burgutin andA. A. Kuchko

7 Somatic Embryogenesis and Protoplast Culture of Polypogon fugaxNees et SteudRsia Chen-au and Chen Dong-fang

8 Regeneration of Somatic Embryos and Plantlets in Protoplast Cultures ofSandalwood (Santalum album L.)P. S. Rao and V, A. Bapat

9 Callus Formation of Mesophyll Protoplasts from Gentiana scabra BungeQian Ying-qian (Y. C. Chien), Zhou Yun-luo, Cai Qi-gui, Zhang Zhi-guo andYan Xian

10 Cultural Studies of Protoplasts Isolated from Cotyledons of Cucumis sativus,Lactuca sativa and Glycine maxLu De Yang, E. C. Cocking, M. R. Davey and D. Pental

11 Regeneration of Plantlets from Mesophyll Protoplasts of Cultivated TomatoFan Shenglan and Ma Yuxia

12 Callus Formation from Cotyledon Protoplasts of Cucumberlia Shirong, Fu Youying and Yang lianrong

13 Culture of Mesophyll Protoplasts from Common Onion (Allium cepa)Wang Guangyuan, Rsia Chenau and Wang Liufa

14 Isolation, Culture and Callus Formation of Sunflower Protoplastslingfen lia and I. Potrykus

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15 Isolation and Culture of Protoplasts in the Grain Legume Vigna radiataR. Majid, N. Gupta and G. K. Kaushik

16 Isolation and Cultural Behaviour of Pollen- Tube SubprotoplastsZhu Cheng, Xie Yi-Ming, Wang Pu and Hu Shi-yi

17 Advances in Plant Genetic ManipulationLi Xianghui

18 Segregation of the Progenies of Interspecific Somatic Hybrids of NicotianaChen Iiayu, Zhao Shimin, Xu Iinxiang; Wang Lianqing and Wang Peitian

19 Isolation, Culture and Fusion of Trifolium ProtoplastsI. W. Grosser and G. B. Collins

20 Plant Regeneration from Mesophyll Protoplasts of Brassica oleracea (Cabbage )Y. Yamashita and K. Shimamoto

VI. MOLECULAR BIOLOGY OF PLANT GENE AND GENETICMANIPULA TION

1 Genetic Engineering as Applied to Plants: Continuation of a Long-term Dispute 228Zhou Guang Yu

2 The Structure of Pea Storage Protein Genes 230D. Boulter, R. R. D. Croy, I. M. Evans, I. A. Gatehouse and G. W. Lycett

3 Purification and Complete Amino Acid Sequencing of B-Subunits of Legumin 232from Pea (Pisum sativum L.)Zhao Wenmingand D. Boulter

4 Cloning and Characterization of Two Coding Sequences for Storage Protein in the 234

Soya-bean (Glycine max)Xue Zhongtian, Xu Meilin, Zhuang Nailiang, Shen Wei and S. C. Shen

(Shen Shanchiang)5 DNA Sequences of Field Bean (Vicia faba) Legumins 236

G. Saalbach, R. lung, C. Lichtenfeld, W. Weschke and U. Wobus6 Synthesis and Cloning of cDNAs Coding for Alfalfa Leghaemoglobins 237

ling Yuxiang7 Brazil Nut Sulphur-rich Protein: Structure and Developmental Regulation 238

S. Sun, S. Altenbach, K. Pearson and F. Leung8 Isolation and Chemical Analysis of Metaphase Chromosomes from a Maize Cell Line 241

Mi lingjiu and Zhou Zhongxin9 Two-dimensional Electrophoretic Analysis of Thylakoid Membrane 242

Polypeptides of a Barley MutantLi ligeng, Li Yuxiang, Gen Yuxuan and Li Iayang

10 Intra- and Interspecific Gene Transfer Using Irradiated Pollen 245Kamla Kant Pandey

11 Phenotypic Variations of Chinese Cabbage (Brassica pekinensis) Induced by Exotic 247DNA of Purple Chinese Cabbage (Brassica chinensis var. purpurea)Zhong Wentian and Shen Minjuan

12 Mutation of Agrobacterium tumefaciens Ti Plasmids Affecting Their Oncogenicity 248Bai Yong-yan, Tang Ti, Mao Hui-zhu and Cao Xiang-ling

13 Molecular Aspects of Early Events in Crown Gall Tumour Formation 249E. Nester, S. Stachel, C. Douglas and M. Gordon

14 Molecular Cloning of Restricted Fragments of Ti Plasmid pTiS1 into Escherichia coli 251Wei Rongxuan, Li Xianghui and Tian Shaocheng

15 Establishment of Soya-bean Cell Lines Containing T -DNA 252Shao Qiquan, liang Xingcun, Zhou Zeqi, Yin Guangchu and

Wang Liangzhen16 Isolation, Restriction Mapping, and Cloning of Rice Chloroplast DNA 253

C. Ma~ill and C. Sheuring

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17 1n-vitro Transcription of Cloned Maize Mitochondrial DNABin Wang and R. I. Mans

18 Gene Transfer in Maize (Zea mays L.)I. C. Waldron, E. S. Dennis, W. I. Peacock, D. I. Llewellyn, E. A. Howardand I. G. Ellis

19 Chromosome-mediated Transformation via MicroinjectionR. I. Griesbach .

20 Regulation of Ribulose-l,5-Bisphosphate Carboxylase Gene Expressionin the C4 Dicot, Grain AmaranthD. F. Klessig, B. I. Nikolau, I. P. Carr and I. 0. Berry

21 Studies in China on Dedifferentiating Cell DivisionLu Wenliang and Kuo Chung-shen

22 Organization and Expression of the Mitochondrial Genome in Beta vulgaris L.L. E.A. Henry, I. I. Hansen and K. S. S9>rensen

23 In-vitro Transformation of Cultured Cells from Daucus carota with Agrobacteriumtumefaciens C58Liu Zhi, Fan Menkang and Cui (Tsui) Cheng

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VII. HAPLOID

1 In-vitro Anther Culture of Fritillaria ussuriensis Maxim and Induction of PollenPlantletsChen Zeguang, Yang Zhentang, Liu Zhidong, Du Lingge, Hou Yanhua,Zhao Yahui, Chang Weichun, Li Ansheng and Shao Qiquan

2 Use of Growth Hormones to Increase the Induction Rate of Anther Cultureof Hsien RiceChen Qing-lian and Zhou Xiong-tao

3 The Induction of Pollen Plants of Litchi (Litchi chinensis Sonn)Fu Lianfang and Tang Daoyi

4 Hybridization of Triticum aestivum with Tetraploid Hordeum bulbosumLi Dawei, Wei Xiuling, Cao Hualin and Hu Qide

5 A Study of the Medium for Direct Induction of Pollen Plantletsin Rice CultureLing Gon-song, Cheu Su-you and Wang Zhon-ko

6 The Salmon Method for Haploid Production in Common WheatK. Tsunewaki, Y. Mukai and M. Yamamori

7 Induction of Pollen Plants of Comfrey (Symphytum officinale Lo )Wang Guanling, Zhang linghua, Wang Xianzhi, Yin Enjie, liang Changyang,Huang ling, Zhang Yanmin and Wang Hongqing

8 Studies on the General Medium for Anther Culture in Rice (0. sativa)Yang Xuerong, Wang lienren, Huan lienzhang, Li Huanling and Li Youfang

9 Induction of Pollen plants of Intergeneric Hybrids of Triticum aestivum X

Triticum-AgropyronZhuang liajun, lia Xu, Chen Guoqingand Sun Shanchen

10 Breeding Fine Rice Strains by Anther CultureFan Ping-yi

11 Anther Culture and Its Application to Purification and Rejuvenation of aMale-sterile Line from Abortive Wild-type RiceGe Meifeng, Tan Changle and Yang Qiangping

12 'Huayu 15' -a High-yielding Rice Variety Developed Through Anther CultureLiang Shouyi and Huang Shouyin

13 Establishment of a Somatic Clone of Sorghum bicolor X Saccharum officinarumProgeny and Characters of the Clone-derived PlantsMu Ouihua. Tian Huixianfl and Zhanfl TianronJ{

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14 Anther Culture 28 -ANew Disease-resistant Line of Winter WheatZhao Youling, He Xianchang, Liu Man and Wang linlan

15 Effect of High-temperature Treatment on Anther Culture in Wheat (Triticumaestivum)Liang Sixin and Hu Han

16 Pollen Androgenesis in Field Bean (Viciafaba)

Thipmani Paratasilpin .17 Anther Culture and Androgenesis in vltro in Glycine max

Yin Guangchu, Zhu Zhiyin, Chen Li and Li Xuezhan18 Induction of Androgenesis in Anther Culture of Alfalfa (Medicago sativa L.)

N. Zagorska and V. Gancheva19 Effect of2-Deoxyglucose on Androgenesis in Rice (Oryza sativa subsp. Keng)

Anther CultureZeng lunzhi

20 In-vitro Culture and Cytological Observation of Unpollinated Ovary fromHybrids of Helianthus tuberosum x Helianthus annuusZhu Zhongchun, Wu Haishan and Qiao Yiaomin

21 Genetic Characters and Breeding Application of Progenies from Hsien Rice viaAnther CultureHan Guang-xi

22 Variation in Chromosome Number of Poplar Plants Obtained by Anther Culturein vitroLu Zhihua, Liu Yuxi and Zhang Peikao

23 Meiotic Studies on Pollen-derived Haploids from Fl Between OctoploidTriticum-Agropyron and Common WheatMiao Zhonghe, Hu Han and Zhuang liajun

24 Preliminary Study on Anther Culture of Hybrid Between Xinjiang Wheat (T.petropauloskyi Udaczet Micush) and Common Wheat (Triticum aestivum)Qi Jiahua, Yie Qinfen and Hu Ru

25 Comparison of Characters in Pollen-derived Hybrid Plants and Conventional F2Hybrids of RiceXu Di-xin, Wang Man-Ii and Hu Qiu-ying

26 Effect of Anther Culture Technique in Seed Production in Keng Rice andGenetic Analysis of Several Characters of PollenXue Qingzhong, Lin ling, Shen Zongtan, Zhang Quande and Gue Quansheng

27 Callus Induction Efficiency of Uni- and Binucleate Pollen of Two Rice Varietiesin Media ElO and G4S. T. Mercy, F. l. Zapata, L. B. Torrizo and R. R. Aldemita

28 New Virus-resistant (TUMV) Lines of Brassica pekinensis Rupr. Through

Haploid BreedingDeng Li Ping, Zeng Ye and Guo Yahua

29 Conditioning Agents for High-frequency Callus and Plantlet Formation fromAnther Cultures of WheatM. D. Lazar, G. W. Schaeffer and P. S. Baenziger

30 Induction of Longan Haploid Plantlets from Cultured PollenYang Yongqing and Wei Wenziong

31 Anther Culture and Plantlet Regeneration in Panax ginseng in vitroDu Lingge, Hou Yanhua, Chang Weichun, Zhao Yahui, Yang Zhentang,Chen Zeguang, Zhang Zengming, Liu Zhidong, Shao Qiquan and

LiAnsheng32 Androgenetic Haploid Induction and Their Use for\Tobacco Improvement

P. Minodora33 Factors Influencing Anther Response to Culture in Barley (Hordeum vulgare)

A. A. Marsolais and K. l. Kasha11Q

"tft1~n~

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34 Some Phenotypic Effects of Ethylmethanesulphonate on Tobacco Pollen PlantsM. Deeburee and T. Paratasilpin

VIII. MUTATION, MUTAGENESIS

1 Cell Survival and Mutation Frequency of Valine Resistance after Gamma-Irradiation of n or 2n N. plumba'ginifolia ProtoplastsE. Nielsen, E. Selva, C. Sghirinzetti af)d M. Devreux

2 In-vitro Induction and Screening of Mutants Resistant to Xanthomonas oryzaefrom Callus Culture in RiceSun Lihua, She Iianming and Lu Xuefeng

3 Para-Aminobenzoic Acid as a Reparagen in Chemical Mutagenesis0. Priilinn and T. Shnaider

4 Selection of Cadmium-tolerant Variants from Rice Cell CulturesLi Hai-min and Chen Ying

5 Tobacco Plants Resistant to Alternaria Toxin Obtained from Protoplast-derivedCallusesI. Furusawa, A. Mizuguchi, P. Thanutong, M. Kodama and S. Nishimura

6 Isolation of Biochemical Mutants in Brassica SpeciesS. I. Molnar and I. L. Grainger

7 Induced Mutations in Anther Culture of WheatZheng Qicheng, Zu Yaolan and Chen Wenhua

8 Production of Gynogenic Doubled Haploids in Oriental LiliesI. Prakash and K. L. Giles

9 Isolation of Some Economic Mutants in Sesame (Sesamum indicum)B. A. Satyan, C. V. Paramesha and P. Gopala Reddy

334

338

338

140

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IX. SOMACLONAL V ARIA TION

1 Chromosomal Behaviour Peculiarities in Two Related Stocks of Maize in vitroV. A. Kunakh and E. K. Savchenko

2 Paraquat-resistant Tobacco Calluses with Enhanced Superoxide Dismutase

Activity1. Furusawa, N. Yamaoka, A. Mizuguchi, K. Tanaka and K. Asada

3 Tissue Culture and Chromosome Analysis of Artemisia lactifloraLi Guozheng, Li Guofeng and Jiang Hongru

4 Establishment of Pollen-Embryonic Cell Clones of Maize (Zea mays) and TheirApplication in BreedingMu Qiuhua

5 Screening for Crop Ideotypes in Somatic Tissue-derived Plants of RiceSong Zongmiao, Liu Zulin and Chen Jingxian

6 Response of Young Panicles of Rice of Various Ploidies to Culture in vitroShu Lihui, Zhang Tingbi and Zhou Mingjie

7 Chromosomal Variation in Tissue Cultures and Regenerated Plants of Urgineaindica KunthS. Jha and ,\,. .\'en

350

x. SOMA TIC MORPHOGENESIS

1 Plantlet Regeneration from Young Ear Cultures of Wheat (Triticum aestivum)and Isolation of Embryonic CellsZhu Qinglin and Zhang Lude

2 Callus Induction and Plantlet Regeneration of Phragmites communisYe Hechun, Wu Guoliang and Li Guofeng

352

CONTENTS XVII

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355

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360

362

363

364

366

368

370

370

371

171

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377

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381

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3 In-vitro Culture of Leaves and Regeneration of Plants of Soya-bean(Glycine max)Yang Zhentang, Chen Zeguang, Liu Zhidong and Zhang Zenging

4 Embryoid Induction and Histocytological Studies on the Callus of Angelicasinensis (Oliv.) DielsZhang Shiyu and Cheng Kuochang

5 Plant Regeneration Through Single-cell Cloning ofCrown Gall Teratomas ofTobacco .Zhang lianming and Zhou liankui

6 Embryoid Regeneration of American Ginseng (Panax quinquefolium) in vitroSun Guodong and Zhang Qi

7 Establishment of Clones, Embryogenesis and Effects of Hormones onOrganogenesis in Elaeagnus angustifolia Lo in vitroPan lingli, Wang Puxuan, Gao Rulan, Fan Hui and Tu Lizhu

8 Tissue Culture of Shoot Apexes of Loquat ( Eriobotrya japonica Lindl)Yang Yongqing, Chen Guanglu and Tang Daoyi

9 The Development of Plantlets from the Endosperm of LoquatChen Zhenguang, Shunquan Lin and Qingliang Lin

10 Differentiation of Organs in Endosperm Culture of Chinese GooseberryGui Yao-lin, Gu Shu-rong and Xu Ting-yu

II Callus Induction and Plantlet Regeneration in GrapeWang Yunzhu, Ge Koulin, Zhou Goazhi, Yang linshui and Ye Mingming

/2 Somatic Embryogenesis and Plant Regeneration from Inflorescence ofEchinochloa crusgalli LoWang Dayuan and Kong Yan

13 Hypocotyl-derived Tissue Culture of M.edicago truncatula and Mo orbicularisLiXo Qo

14 In-vitro Regeneration of Plantlets from Immature Endosperms of Maize(Zea mays)Zhu Qinglin, Chen Xinmin, Li Wenxian and Chen Yofen

15 Quick Propagation of Guayule in vitroXu Huizhu and Qian Minzhi

16 Regeneration of Virus-free Plants from Inflorescences of Virus-infectedGladiolusZheng Wan Zhen, Huang liao Xiang, Guan Yu Lan, He Zhuan Qi,Xi Zhong Xing, Xu Shan Hwa, Cai Wen Qi, Zhao Shu Zhen and Mang Ke liang

17 Inflorescence Culture in vitro for Rapid Propagation of Hosta plantaginea var .plena Hort. (Fragrant Plantain Lily)Huang liao Xiang, Zheng Wan Zhen, Guan Yu Lan, Lung Ya Yi,Gong Wei Zhong, Fei Yan Liang and Wu En Hwa

18 Regeneration of Barley-Wheat Hybrid Plants from Immature Embryos andInflorescencesHsieh Su-Lun, Xu lie and Zhong Yu-Quan

19 Tissue Culture ~s a Tool in Soya-bean ImprovementEo lo Roth, Ro lo Honeycutt, Ko Go Lark, Ho Eo Heer and Ro Go Palmer

20 Chromosome Numbers of Endosperm Plants of Chinese WolfberryWang Li, Chen Shupin, Qin linsan, Wang Dazhen, Shao Qiquan andNiu Deshuei

21 Induction of Embryoids and Plants from Sta~ens and Unripe Seeds of LongYellow Day-Lily in vitroZhou Puhua, Hu lijin and Fan Hongzhi

22 Suspension Culture and Organic Differentiation of Populus CellsHu Qianiin, Han Yifan and Xu Weiyin

CONTENTSXVIII

23 Preliminary Establishment of Embryoid Clones of Yanhusuo (Corydalis remeta 384

Fisch. ex Maxim)Li Ansheng, Shao Qiquan, Du Lingge, Hou Yanhua, Chang Weichun,Chen Zeguang and Yang Zhentang

24 Plant Regeneration from Leaf Discs of Tomato 386Iia Shirong, Fu Youying, Yang Iianrong and Cheng Yun

25 Propagation of Chinese Gooseberry from Stem Explants in vitro 388Huang Zhenguang, Huangfu Youli, Xu Leyin and Wang Qimin

26 Micropropagation of Peony -_JLi Yulong, Wu Deyu, Pan Shulong, Xu Shaoli, Wei Zhiming and Xu Zhihong

27 Virus-free Sweet Potato by Shoot-tip Culture 390

Luo Hong yuan28 Callus Formation and Plant Regeneration from Young Stem Segments of JJ.I

Ricinus communis L.Zhou Genyu

29 Tissue Culture and Plant Regeneration of Banana --.

Ouyang Shu30 Induction of Embryoids and Plantlets from Oil Palm Callus JJJ

Cui Yuanfang, Gong Zheng, Chen Xinghua, Shao Xingdi and Zhu Zhiqing

XI. PROTOPLASTS1 Mesophyll Protoplast Fusion Induced by External Electric Fields -~~

Song Yonggen, Hsia Chen-au and Zhang Lingxiang2 Fusion of Male Sterile Protoplasts with Fertile Protoplasts JJJ

Yasuyuki Yamada and Yang Zhi-Qi3 Uptake of N2-fixing Cyanobacterium Gloeocapsa by Plant and Fungal Protoplasts ~v~

Chen Tingwei, Xie Yingxian, Chen Wanhua and Zhou Hongbin4 Interspecific Cell Hybrids and Their Succeeding Generations in Tobacco .vo

Gong Minliang, Luo Qizhang and Bu Guozhang5 Protoplast Isolation and Culture of Musk Melon (Cucumis melo var. makuwa) -.v-

and Hami Melon (Cucumis melo var. sol charinus)Zhang Da Li

6 Protoplast Culture of Mustard (Brassicajuncea) -.v-Li Wenbin, Chen Zhizheng, Zhang Dawei and Chen Zhenghua

7 Cultures of Leaf and Mesophyll Protoplasts of Astragalus sinicus Linn. -..,.

Gao Ming-xing and Xu Zhi-hong8 Plant Regeneration from Callus Derived from Isolated Protoplasts of Winged ~v,

Bean (Psophocarpus tetragonolobus)W. M. Wilson, N. Haq and P. K. Evans

9 In-vitro Production of Potato in China ~...Tao Guoqing, Yin Weiyi, Cui Cheng and Gong Guopu

10 Towards Somatic Hybridization of Clovers ~~I. Honkanen, P, Ryoppy and P. M. A. Tigerstedt

11 Isolation and Culture of Bract Protoplasts of Banana ..K. Matsumoto, L. Crepy, I. B. Teixeira and F. R. Ferreira

12 Applications of Protoplast Fusion to Plant Breeding ~~V. A. Evans, I. E. Bravo and S. S. Chase

XU. MOLECULAR BIOLOGY OF PLANT GENE AND GENETICMANIPULA TION

1 Teratoma Induction and Gene Transfer in Sugar-beet ~..Wang Yuqin, Liuo Chuie, Lian Youngyun, Shao Qiquan, Iiang Xingcun and

Zhou Zheai

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.-, Transfer and Teratoma Induction in Brassica oleracea L.liang Xingcun, Shao Qiquan and Zhou ZeqiInduction of Pollen Plants in Glycine max (L.) Merr .lian Yuyu, Liu Dupu, Luo Ximing and Zhao GuilanThe Teratomas of Solanum tuberosum and Brassica campestris and Regenerationof Tumour Tissues in vitroQin Changle and Li Xianghui .

5 In-vitro Study of Mitochondrial Protein SynthesisXie Youju, K. Storey and B. Gengenbach

6 Expression of Genes Coding for Vicia faba Storage ProteinsG. Saalbach, R. BassUner, H. Biiumlein, Ch. Horstmann,R. lung, C. Lichtenfeld, K. MUntz, W. Weschke and u. Wobus

7 Variations of Rice (Oryza sativa) Induced by Foreign DNA UptakeDuan Xiaolan and Chen Shanbao

8 Teratoma Induction in Sugar-beetLiuo Chuie, Wuang Yuqin, Li Yongfeng, Shao Qiquan, liang Xingcun andZhou Zheqi

9 The Use of DNA Methylases to Alter the Apparent Recognition Specificities ofRestriction EndonucleasesM. Nelson, C. Christ and I. Schildkraut

10 Differential Replication of Mitochondrial DNA in Hazel (Corylus avellana)Callus Cultures During Phytohormone-dependent Callogenic andOrganogenic DevelopmentF. Bernard-Durix and A. Kovoor

11 The Tumour-induced Reaction of Glycine Infected by Agrobacterium tumefaciensand T-DNA Transfer .

lian Yuyu, Zheng Huiyu, Su Zhongwu, Shao Qiquan, liang Xingcun andZhou Zeqi

Inclex of Authors 419