year: first year semester: i course: microbial diversity and ......year: first year course:...

Year: First Year

Course: Microbial Diversity and Taxonomy

Teaching

Scheme

(Hrs/Week)

Continuous Internal Assessment

L T P C CIA-1 CIA-2

4 0 - 4 10 20

Max. Time, End Semester Exam (Theory)

Prerequisite • Introduction

Objectives

1 To expand knowledge on Evolution and microbial diversity

2 To understand bacterial taxonomy

3 To study various classes of fungi in terms of their characteristic features.

4 To assimilate the concepts of unculturable bacterial diversity

5 To expand knowledge on Evolution and microbial diversity

Unit

Number

1

Differences in concept

Definition of species in prokaryotes.

Types of ‘species’

Evolution of species and concepts of speciation (in sexual

organisms)

Types of evolution (neutral, co

k selection; molecular clocks; phylogeny

2

The expanse of microbial diversity

Estimates of total number of species

Species Divergence and the measurement of microbial diversity.

Measures and indices of diversity

3

Introduction to Bacterial Taxonomy

The 5-Kingdom classification system

The 3-Domain classification system

Bergey’s Manuals and the classification of prokaryotes.

Determinative Bacteriology (Phenetic Approach)

Systematic Bacteriology (Phylogenetic

Polyphasic Approach

School of Science M.Sc. Microbiology

Semester: I

Microbial Diversity and Taxonomy Course Code:

Continuous Internal Assessment (CIA) End Semester

Examination

2 CIA-3 CIA-4 Lab Theory Lab

10 10 - 50 -

End Semester Exam (Theory) - 3Hrs.

Introduction to microbiological concepts, terms etc.

To expand knowledge on Evolution and microbial diversity.

To understand bacterial taxonomy.

study various classes of fungi in terms of their characteristic features.

To assimilate the concepts of unculturable bacterial diversity.

To expand knowledge on Evolution and microbial diversity.

Details

Differences in concept of ‘species’ in eukaryotes and prokaryotes.

Definition of species in prokaryotes.

Evolution of species and concepts of speciation (in sexual and asexual

Types of evolution (neutral, co-evolution); Types and levels of selectio

k selection; molecular clocks; phylogeny and molecular distances

The expanse of microbial diversity

Estimates of total number of species

Species Divergence and the measurement of microbial diversity.

Measures and indices of diversity

Introduction to Bacterial Taxonomy

Kingdom classification system

Domain classification system

Bergey’s Manuals and the classification of prokaryotes.

Determinative Bacteriology (Phenetic Approach)

Systematic Bacteriology (Phylogenetic Approach

Pa

ge1

Semester: I

Course Code: PMI101

Total

100

Hours

and asexual

of selection; r and

12 L

12 L

12 L

Course Outcome

Students should able to

CO1 Student will be able to understand the concepts

nomenclature.

CO2 Student will be able to

microorganisms from variety of


methods.


microbial species from different sources.

CO5 Student will be able to understand

4

The 6 Classes of Fungi.

The differentiating characters among different Classes of fungi.

The importance of morphological characters in fungal differentiation and

classification.

5

Concept of ‘unculturable’

Strategies for culture of ‘unculturable’ bacteria.

Culture independent molecular methods for identifying unculturable bacteria.

Methods of extracting total bacterial DNA from a habitat and metagenome

analysis.

Resources

Recommended

Books

1. Jacquelyn G. Black (2013) Microbiology: Principles and

6th Edition, John Wiley & Sons, Inc.,

2. Microbial Diversity: Form and Function in

Online: 30 NOV 2007.

3. Ridley Mark (2004). Evolution. Blackwell Science Ltd.

4. Breed and Buchanan. Bergey’s Manual of Determinative

8th Edition, 1974.

5. Breed and Buchanan. Bergey’s Manual of

9th Edition, 1982.

6. Breed and Buchanan. Bergey’s Manual of Systematic

Edition, (Volumes. 1

Reference

Books

1. Sykes, G. and F. A. Skinner (Eds). Actinomycetales:

Practical

2. Bacteriology Symposium Series No. 2, Academic Press. 1973.

3. Jacquelyn G. Black (2013) Microbiology: Principles and


4. Lodder J. (1974). The Yeasts: A Taxonomic Study, North

Publishing Co. Amsterdam.

Student will be able to understand the concepts microbial taxonomy and methods of

Student will be able to use the knowledge for isolation and identification of

microorganisms from variety of sources.

Student will be able to classify the microorganisms based on classical and advanced

Student will be able to understand microbial diversity, species and estimation of various

microbial species from different sources.

Student will be able to understand evolution of microbial species.

The 6 Classes of Fungi.

The differentiating characters among different Classes of fungi.


Concept of ‘unculturable’ bacterial diversity.

Strategies for culture of ‘unculturable’ bacteria.



Jacquelyn G. Black (2013) Microbiology: Principles and


Microbial Diversity: Form and Function in Prokaryotes,

Online: 30 NOV 2007. DOI: 10.1002/9780470750490.ch1

Ridley Mark (2004). Evolution. Blackwell Science Ltd.

Breed and Buchanan. Bergey’s Manual of Determinative

8th Edition, 1974.

Breed and Buchanan. Bergey’s Manual of Determinative

9th Edition, 1982.

Breed and Buchanan. Bergey’s Manual of Systematic

Edition, (Volumes. 1 – 5) (2001 – 2003).

Sykes, G. and F. A. Skinner (Eds). Actinomycetales: Characteristics and

Practical Importance. Society for Applied

Bacteriology Symposium Series No. 2, Academic Press. 1973.

Jacquelyn G. Black (2013) Microbiology: Principles and


Lodder J. (1974). The Yeasts: A Taxonomic Study, North

Publishing Co. Amsterdam.

Pa

ge2

microbial taxonomy and methods of

use the knowledge for isolation and identification of

classify the microorganisms based on classical and advanced

understand microbial diversity, species and estimation of various


12 L



12 L

Total 60

Jacquelyn G. Black (2013) Microbiology: Principles and Explorations,

Prokaryotes, Published

DOI: 10.1002/9780470750490.ch1

Ridley Mark (2004). Evolution. Blackwell Science Ltd.

Breed and Buchanan. Bergey’s Manual of Determinative Bacteriology.

Determinative Bacteriology.

Breed and Buchanan. Bergey’s Manual of Systematic Bacteriology. 2nd

Characteristics and

Bacteriology Symposium Series No. 2, Academic Press. 1973.

Jacquelyn G. Black (2013) Microbiology: Principles and Explorations,

Lodder J. (1974). The Yeasts: A Taxonomic Study, North Holland

Year: First Year

Course: Instrumentation and Molecular Biophysics

Teaching

Scheme

(Hrs/Week)

Continuous Internal Assessment (CIA)

L T P C CIA-1 CIA-2

4 0 - 4 10 20


Prerequisite • Introduction and basic concepts of

• Basic concept

Objectives

1 To create general understanding of

2 To familiarize the students with principles of bioinstrumentation used in qualitative and

quantitative analysis.

3 To develop the concepts and train the students to increase their competency

4 To develop analytical skills of students

5 To orient students towards biological research with the help of bioinstrumentation

techniques.

Unit

Number

1 Laboratory Instruments:

meter, Laminar air flow, Centrifuge machine types and Centrifugation:

Differential, Rate Zonal, Isopycnic, Density gradient, Rotor types and Ultra

centrifugation. Phase Contrast Microscope; Fluorescent Microscope; Scanning

and Transmission Electron Microscopy.

2 Chromatography Techniques

of Paper Chromatography,TLC, HPTLC, Gel Filtration Chromatography, Ion

Exchange Chromatography, Affinity Chromatography, Gas Chromatography,

and HPLC.

Electrophoretic Techniques

Electrophoresis, Poly Acrylamide Gel Electrophoresis (PAGE), Agarose Gel

Electrophoresis. Principle and Applications of: Iso

3

Radio-isotopic Techniques

Biological Applications, Radioactive Decay

Principles and Applications of GM (Geiger Muller) Counter, Solid and Liquid

Scintillation Counter, Autoradiography, Radioimmunoassay (RIA), Radiation

Dosimeters.

School of Science M.Sc. Microbiology

Semester: I

Instrumentation and Molecular Biophysics Course Code:


Examination


10 10 - 50 -


Introduction and basic concepts of instrumentation, analytical techniques

Basic concept related to biochemical and biophysical methods

understanding of various bioanalytical methods.

To familiarize the students with principles of bioinstrumentation used in qualitative and

To develop the concepts and train the students to increase their competency

develop analytical skills of students.

To orient students towards biological research with the help of bioinstrumentation

Details

Laboratory Instruments: Theory, Principle, Working and applications of: pH

Laminar air flow, Centrifuge machine types and Centrifugation:



and Transmission Electron Microscopy.

Chromatography Techniques: Theory, principle, operation and applications



Electrophoretic Techniques: Theory, Principle and Applications of Paper


Electrophoresis. Principle and Applications of: Iso-electric Focusing

isotopic Techniques : Introduction to Radioisotopes and th

Biological Applications, Radioactive Decay–Types and Measurement,



Pa

ge1

Semester: I

Course Code: PMI102

Total

100

instrumentation, analytical techniques

related to biochemical and biophysical methods

To familiarize the students with principles of bioinstrumentation used in qualitative and

To develop the concepts and train the students to increase their competency.

To orient students towards biological research with the help of bioinstrumentation

Hours

Theory, Principle, Working and applications of: pH

Laminar air flow, Centrifuge machine types and Centrifugation:



1

: Theory, principle, operation and applications



heory, Principle and Applications of Paper


2

: Introduction to Radioisotopes and their

Types and Measurement,



3

4 Molecular Biophysics:

of size of proteins, Physical nature of non

Conformational properties of proteins, Ramachandran plot, secondary, super

secondary, tertiary and quaternary structures of

dimensional structures of proteins (motifs and fold domains) Protein

structure/properties determination.

5 Spectroscopies of Biomolecules and Biophysical Techniques

1. UV/Visible spectroscopy

Lamberts Law, Bathochromic and hypsochromic shifts.

2. Fluorescence spectroscopy

FRET, Binding and Folding studies.

3. Infrared spectroscopy

and its advantages

4. Circular Dichroism (CD)

absorbance, Cotton Effect.

5. Mass spectroscopy: Principles of operation and types of spectrometers,

ionization, Ion fragmentation, Mass analyzers, GC

MALDI-TOF

6. X-ray crystallography: Isolation and purification of proteins, crystallization

of proteins, instrumentation, acquisition of the diffraction pattern, basic

principles of x-ray diffraction, Phase determination

7. NMR spectroscopy: Basic Principles of NMR, Chemical shift, Intensity, Line

width, Relaxation parameters, Spin

NMR Applications in Biology

Course Outcome


CO1 Student will be able to demonstrate an understanding

required in biological processes.

CO2 Student will be able to demonstrate the basic

bilogical studies.

CO3 Student will be able to demonstrate the basic idea and applications of

techniques

CO4 Student will be able to introduce

these concepts and ideas in technology


approach.

Resources

Recommended

Books

• Clive Dennison (2002)

Publishers

• Pattabhi, V. and Gautham, N. (2002)

Publishers, New York and NarosaPublishingHouse, Delhi.

Biophysics:Theoretical and experimental methods for determination

of size of proteins, Physical nature of non-covalent interactions,

Conformational properties of proteins, Ramachandran plot, secondary, super

secondary, tertiary and quaternary structures of proteins, Classification of three


structure/properties determination.

Spectroscopies of Biomolecules and Biophysical Techniques

UV/Visible spectroscopy- Instrumentation, Molar Absorptivities, Beer and

Lamberts Law, Bathochromic and hypsochromic shifts.

2. Fluorescence spectroscopy- Instrumentation, Quantum Yield, Quenching,

FRET, Binding and Folding studies.

Infrared spectroscopy- Principle , Instrumentation, Absorption bands

4. Circular Dichroism (CD) – Instrumentation, Circular polarization, Delta

absorbance, Cotton Effect.


ionization, Ion fragmentation, Mass analyzers, GC-MS, Biological applications,

ray crystallography: Isolation and purification of proteins, crystallization


ray diffraction, Phase determination

spectroscopy: Basic Principles of NMR, Chemical shift, Intensity, Line

width, Relaxation parameters, Spin-spin coupling, Nuclear Overhauser Effect,

NMR Applications in Biology

Student will be able to demonstrate an understanding of various instruments that are

required in biological processes.

Student will be able to demonstrate the basic understanding of the instruments used for

to demonstrate the basic idea and applications of

Student will be able to introduce principle of basic techniques and train them to apply

these concepts and ideas in technology .

Student will be able touse the knowlegde of techniques and develop the problem

Clive Dennison (2002) A guide to protein isolation, KluwerAcademic

Pattabhi, V. and Gautham, N. (2002) Biophysics.

New York and NarosaPublishingHouse, Delhi.

Pa

ge2

Theoretical and experimental methods for determination

covalent interactions,

Conformational properties of proteins, Ramachandran plot, secondary, super-

proteins, Classification of three


4

Absorptivities, Beer and

Instrumentation, Quantum Yield, Quenching,

Principle , Instrumentation, Absorption bands, FTIR

Instrumentation, Circular polarization, Delta


Biological applications,

ray crystallography: Isolation and purification of proteins, crystallization


spectroscopy: Basic Principles of NMR, Chemical shift, Intensity, Line

spin coupling, Nuclear Overhauser Effect,

5

Total 60

of various instruments that are

of the instruments used for

to demonstrate the basic idea and applications of the instruments or

basic techniques and train them to apply

knowlegde of techniques and develop the problem-solving

, KluwerAcademic

Biophysics. KluwerAcademic

• David J Holme, Hazel Peck (1998)

Prentice Hall, Pearson Education Limited, HarlowEngland.

• Rodney F. Boyer (2000

edition,Benjamin Cummings.

• Nölting, B. (2006)

Germany.

• Wilson Keith and Walker John (2005)

Biochemistry and Molecular Biology,

New York.

• Pattabhi, V. and Gautha


• Rolf Ekman, Jerzy Silberring, Ann Westman

(2009) Mass spectrometry: instrumentation,interpretation, and applications

John Wiley & Sons

• Irwin H. Segel (1976)

SolveMathematical Problems in General Biochemistry,

Wiley & Sons.


Germany.

• Pattabhi, V. an


• Cavanagh John

Practice, Academic Press.

• Keeler, J. (2002)

England.

• Drenth, J. (2007)

Springer, Germany.


Springer,Germany.

• Cotterill, R. M. J. (2002)

England.

Reference Books

• David J Holme, Hazel Peck (1998)


• Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006)

Edition. Freeman, New York.

• Garrett, R. H. and Grisham, C. M. (2004)

Cole, Publishing Company, California

• Cotterill, R. M. J. (2002)

England.

• Christof M. Niemeyer and Chad A. Mirkin

Wiley & Sons.

• Daniel L. Feldheim and Colby A. Foss, Jr.

synthesis and characterization and applications

• Mahendra Rai and Nelson Duran (2011)

inMicrobiology

David J Holme, Hazel Peck (1998) Analytical Biochemistry


Rodney F. Boyer (2000) Modern Experimental Biochemistry

edition,Benjamin Cummings.

Nölting, B. (2006) Methods in modern biophysics. SecondEdition. Springer,

Wilson Keith and Walker John (2005) Principles andTechniques of

Biochemistry and Molecular Biology, 6th Ed.Cambridge University Press,



Rolf Ekman, Jerzy Silberring, Ann Westman-Brinkmalm,AgnieszkaKraj

Mass spectrometry: instrumentation,interpretation, and applications

John Wiley & Sons,Inc.,Canada.

Irwin H. Segel (1976) Biochemical Calculations: How to

SolveMathematical Problems in General Biochemistry,

Wiley & Sons.

Nölting, B. (2006) Methods in modern biophysics. SecondEdition. Springer,



Cavanagh John et.al. (1995) Proteins NMR Spectroscopy:Principles and

, Academic Press.

Keeler, J. (2002) Understanding NMR Spectroscopy. JohnWiley& Sons,

Drenth, J. (2007) Principles of protein X-ray crystallography.

Springer, Germany.

Nölting, B. (2006) Methods in modern biophysics.

Springer,Germany.

Cotterill, R. M. J. (2002) Biophysics: An Introduction. JohnWiley& Sons,

David J Holme, Hazel Peck (1998) Analytical Biochemistry


Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006)

Freeman, New York.

Garrett, R. H. and Grisham, C. M. (2004) Biochemistry.

Cole, Publishing Company, California

Cotterill, R. M. J. (2002) Biophysics: An Introduction. JohnWiley& Sons,

Christof M. Niemeyer and Chad A. Mirkin (2006)Nanobiotechnology

Wiley & Sons.

Daniel L. Feldheim and Colby A. Foss, Jr. (2002) Metalnanoparticles

synthesis and characterization and applicationsMarcel Dekker, Inc.

Mahendra Rai and Nelson Duran (2011) Metal nanoparticles

inMicrobiology, Springer Verlag Berlin Heidelberg.

Pa

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Analytical Biochemistry,3rded .,

) Modern Experimental Biochemistry3d

SecondEdition. Springer,

Principles andTechniques of

6th Ed.Cambridge University Press,


Brinkmalm,AgnieszkaKraj

Mass spectrometry: instrumentation,interpretation, and applications,

Biochemical Calculations: How to

SolveMathematical Problems in General Biochemistry, 2nd Edition.John

SecondEdition. Springer,


Proteins NMR Spectroscopy:Principles and

JohnWiley& Sons,

ray crystallography.3rd Ed.

Methods in modern biophysics. SecondEdition.

JohnWiley& Sons,

Analytical Biochemistry,3rd Ed.,

Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006)Biochemistry. 6th

Biochemistry. 3rd

Ed. Brooks/

JohnWiley& Sons,

Nanobiotechnology, John

(2002) Metalnanoparticles

Marcel Dekker, Inc.

Metal nanoparticles

Year: First Year

Course: Cell and Molecular Biology

Teaching

Scheme

(Hrs/Week)


L T P C CIA-1 CIA-2

4 0 - 4 10 20


Prerequisite • Introduction and basic concepts of

Objectives

1 To introduce the fundamental

molecular level.

2 To familiarize the students with the importance of cellular and molecular process for

encouragement towards research and develop scientific thinking

3 To develop proficiency and technical skills of students.

4 To apply the knowledge

various problems.

5 To develop research driven approach for better understanding of

biological processes.

Unit

Number

1

Structural organization Membrane and Communication of cell

1.1- Introduction, History and General organization of Prokaryotic cell

Mycoplasma, Viruses, Viroids and Bacteria (

1.2 General organization of Eukaryotic cell (Plant cell)

1.3 Composition of cell membrane

1.4 Molecular modelsmosaic Model.

1.5 Functions of cell membrane

1.6General principles of cell signaling.

1.7 G-protein linked receptors.

1.8 Enzyme linked receptors.

1.9 Mitosis and Meiosis

Structural organization, functions of cell organelles and Cytoskeleton

2.1 Nucleus-Ultrastructure, Nuclear envelop, Pore complex

School of Science

M.Sc. Microbiology

Semester: I

Cell and Molecular Biology Course Code:


Examination


10 10 - 50 -


Introduction and basic concepts of living cell, cellular system etc

fundamental concepts and processes of living organisms at cellular

To familiarize the students with the importance of cellular and molecular process for

encouragement towards research and develop scientific thinking.

To develop proficiency and technical skills of students.

pply the knowledge cellular and molecular mechanism of biological system

develop research driven approach for better understanding of

Details

Structural organization Membrane and Communication of cell

Introduction, History and General organization of Prokaryotic cell

Mycoplasma, Viruses, Viroids and Bacteria (E.coli).

1.2 General organization of Eukaryotic cell (Plant cell)

1.3 Composition of cell membrane

Molecular models-Unit membrane, Daniely and Dowson͛s Model

1.5 Functions of cell membrane

1.6General principles of cell signaling.

protein linked receptors.

1.8 Enzyme linked receptors.

1.9 Mitosis and Meiosis

Structural organization, functions of cell organelles and Cytoskeleton

Ultrastructure, Nuclear envelop, Pore complex Pa

ge1

Semester: I

Course Code: PMI103

Total

100

living cell, cellular system etc.

and processes of living organisms at cellular and

To familiarize the students with the importance of cellular and molecular process for

cellular and molecular mechanism of biological system to address

develop research driven approach for better understanding of the applications of

Hours

Introduction, History and General organization of Prokaryotic cell-

Daniely and Dowson͛s Model, Fluid

12 L

Structural organization, functions of cell organelles and Cytoskeleton 12 L

2 2.2 Mitochondria-Morphology, molecular structure, membranes, molecular

organization, Biogene

Genetic code.

2.3 Chloroplast-Morphology, Ultra structure, molecular organization of

thylakoids, Biogenesis, DNA, Semi

2.4 Golgi complex- Morphology, cytochemistry

2.5 Endoplasmic reticulum

3

Structural organization, functions of cell organelles and:

Cytoskeleton: 3.1Ribosomes

3.2Structure and functions of Lysosomes, Vacuoles, Peroxisomes.

3.3Microtubules-cillia, flagella, & centrioles.

3.4Intermediate filaments.

3.5Microfilaments and cell motility.

4

Deoxyribose Nucleic Acid

4.1 DNA-chemical composition, bases, sugar, phosphate, Nucleosides,

Nucleotides, Polynucleotides, Bonding systems, Watson and Crick double

helix model of DNA, Types

4.2 DNA Replication

Origin of replication (OriC in

synthesis, Leading strand, Lagging strand, Okazaki fragments, prokaryotic

and Eukaryotic enzymes involved in DNA

Eukaryotic enzymes involved in DNA

bidirectional DNA- replication,

4.3-Replication in closed circular DNA

replication.

4.4-DNA-damages and Repair:

4.6 Significance of DNA

5

Ribose Nucleic acid, Gene Regulation and Transposons

5.1-Structure and functions of tRNA, rRNA, mRNA

5.2-Transcription-Capping, elongation, and termination, Role of RNA

polymerases.

5.3-Processing of RNA

5.4 Constitutive and regulated genes, Positive and negative con

transcriptional control, translational control, and post

5.5 Gene regulation in Prokaryotes

operon-trp operon.

Course Outcome


CO1 Student will be able to d

CO2 Student will be able to e

Morphology, molecular structure, membranes, molecular

organization, Biogenesis, DNA, Symbiotic hypothesis Termination codon,

Morphology, Ultra structure, molecular organization of

thylakoids, Biogenesis, DNA, Semi-autonomous nature.

Morphology, cytochemistry

reticulum-Morphology, Biogenesis, protein segregation

Structural organization, functions of cell organelles and:

3.1Ribosomes-types, structure.

ctions of Lysosomes, Vacuoles, Peroxisomes.

a, flagella, & centrioles.

3.4Intermediate filaments.

3.5Microfilaments and cell motility.

Deoxyribose Nucleic Acid

chemical composition, bases, sugar, phosphate, Nucleosides,


helix model of DNA, Types-A, B, and Z DNA.

4.2 DNA Replication-Replicon, Replisome, Primosome, Replication fork,

Origin of replication (OriC in E. coli) RNA-primer, Semidiscontinuos

nthesis, Leading strand, Lagging strand, Okazaki fragments, prokaryotic

and Eukaryotic enzymes involved in DNA- fragments, prokaryotic and

Eukaryotic enzymes involved in DNA- replication, Unidirectional and

replication,

in closed circular DNA- θ-mode, σ-mode and D

damages and Repair:-Damages-Dimer formations, mismatch pair,

4.6 Significance of DNA.

Ribose Nucleic acid, Gene Regulation and Transposons

Structure and functions of tRNA, rRNA, mRNA

Capping, elongation, and termination, Role of RNA

Processing of RNA- RNA-editing, splicing, polyadenylation

5.4 Constitutive and regulated genes, Positive and negative con

transcriptional control, translational control, and post-translational control

5.5 Gene regulation in Prokaryotes- Inducible operon-lac operon, Represible

be able to describe fundamental processes of cell.

Student will be able to explain the cellular processes of biological system

Pa

ge2

Morphology, molecular structure, membranes, molecular

sis, DNA, Symbiotic hypothesis Termination codon,

Morphology, Ultra structure, molecular organization of

Morphology, Biogenesis, protein segregation.

12 L

chemical composition, bases, sugar, phosphate, Nucleosides,


Replicon, Replisome, Primosome, Replication fork,

primer, Semidiscontinuos

nthesis, Leading strand, Lagging strand, Okazaki fragments, prokaryotic

fragments, prokaryotic and

replication, Unidirectional and

mode and D-loop

Dimer formations, mismatch pair,

12 L

Capping, elongation, and termination, Role of RNA-

5.4 Constitutive and regulated genes, Positive and negative control,

translational control

lac operon, Represible

12 L

Total 60

cellular processes of biological system


system.


sciences


of biological challenges

Resources

Recommended

Books

1. Strickberger. Genetics. 3rd Ed. 1990.

2. A. K. Sharma and A. Sharma. 1990. Chromosome techniques

3. P.K. Gupta. 1990. Genetics.

4. Principles & Methods in Plant Molecular

Biology,Genetics&Biochemistry,Agrobios. Prathibha Devi

5. A. K. Sharma and A. Sharma. 1990. Chromosome techniques.

Reference Books

1. E.D.P. De Robertis and E. M. F. De Robertis. 1987. Cell and Molecular

biology8th Ed(Indian Ed

2. G. M. Cooper. 1997. The Cell and Molecular approach. ASM Press. Ed.

3. Snustad and Simmons. 1997. Principles of Genetics. Ed.

4.Benjamin Lew

5. Daniel Hartl. 1994. Basic Genetics. Ed

6.Winter, Hicky and Fletcher. 1999. Instant notes in Genetics. Ed.

7.A.V.S.S. Sambamurthy. 1999. Genetics.

8.P.K. Gupta. 1990. Genetics.

9. Twyman. 1998. Advanced Molecular Biology.

10.Turner,

MolecularBiology.

11.Primrose. 1999. Molecular Biotechnology.

12.Stansfield. 1996. Theory & Problems in Genetics. Schaum’s Series.

McGraw & Hill.

Student will be able to understand the molecular mechanism involved in

Student will be able to apply the knowledge to solve problems related to biological

Student will be able to understand gene regulation mechanism to address number

of biological challenges

1. Strickberger. Genetics. 3rd Ed. 1990. Ed.

A. K. Sharma and A. Sharma. 1990. Chromosome techniques

P.K. Gupta. 1990. Genetics.

Principles & Methods in Plant Molecular

Biology,Genetics&Biochemistry,Agrobios. Prathibha Devi


E.D.P. De Robertis and E. M. F. De Robertis. 1987. Cell and Molecular

biology8th Ed(Indian Ed


3. Snustad and Simmons. 1997. Principles of Genetics. Ed.

4.Benjamin Lewis. 1999. Genes VII.

5. Daniel Hartl. 1994. Basic Genetics. Ed


7.A.V.S.S. Sambamurthy. 1999. Genetics.

8.P.K. Gupta. 1990. Genetics.

9. Twyman. 1998. Advanced Molecular Biology.

10.Turner, Mclennon, Bates and White. 1999. Instant notes in

MolecularBiology.

11.Primrose. 1999. Molecular Biotechnology.


McGraw & Hill.

Pa

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understand the molecular mechanism involved in biological

related to biological

understand gene regulation mechanism to address number

A. K. Sharma and A. Sharma. 1990. Chromosome techniques

Principles & Methods in Plant Molecular


E.D.P. De Robertis and E. M. F. De Robertis. 1987. Cell and Molecular



Mclennon, Bates and White. 1999. Instant notes in


Year: First Year

Course: Enzymes and Microbial Metabolism

Teaching

Scheme

(Hrs/Week)


L T P C CIA-1 CIA-2

4 0 - 4 10 20


Prerequisite 1. Introduction and basic concepts of

2. Basic concept

Objectives

1 To impart the knowledge of microbial physiology and biochemistry that

develop the concepts and skills related to subject.

2 To understand and critically evaluate research that addresses physiological and

biochemical processes of microbes.

3 To encourage application of the theories related to microbial metabolism

bio-products and to address current problems.

4 To impart the knowledge of

5 To develop an understanding of metabolic processes carried out in all kind of living

systems

Unit

Number

1 Enzyme Kinetics

Purifications of enzyme, purification chart, kinetics of single substrate

enzyme catalyzed reaction.

Kinetics of reversible inhibitions enzyme catalyzed reactions, King Altman

approach to derive –

substrate enzyme catalyzed reactions, concept of allosterism, positive and

negative co-operatively, models of allosteric enzymes (Monod, Wyamann

and Changuaxmodel,Koshland, Nemethy and Filmer model), kinetics of

allosteric

enzyme, Hill plot, examples of allosteric enzymes and their significance in

allosteric regulation

2 Bio-energetic

Laws of thermodynamics, entropy, enthalpy, free energy, freeenergy and

equilibrium constant, Gibbs free energy equation,determination o

School of Science

M.Sc. Microbiology

Semester: I

Microbial Metabolism Course Code:


Examination


10 10 - 50 -


Introduction and basic concepts of enzymes

Basic concept of microbial physiology

To impart the knowledge of microbial physiology and biochemistry that

develop the concepts and skills related to subject.

To understand and critically evaluate research that addresses physiological and

biochemical processes of microbes.

To encourage application of the theories related to microbial metabolism

products and to address current problems.

To impart the knowledge of enzymes, its kinetics, extraction and applications.

develop an understanding of metabolic processes carried out in all kind of living

Details


enzyme catalyzed reaction.


two substrate enzyme catalyzed reactions, types of two


operatively, models of allosteric enzymes (Monod, Wyamann




equilibrium constant, Gibbs free energy equation,determination o

Semester: I

Course Code: PMI104

Total

100

To impart the knowledge of microbial physiology and biochemistry that needed to

To understand and critically evaluate research that addresses physiological and

To encourage application of the theories related to microbial metabolism for production of

enzymes, its kinetics, extraction and applications.

develop an understanding of metabolic processes carried out in all kind of living

Hours



catalyzed reactions, types of two


operatively, models of allosteric enzymes (Monod, Wyamann



12 L


equilibrium constant, Gibbs free energy equation,determination of free

12 L

energy of hydrolytic and biological oxidation reduction reactions, under

standard and non-standardconditions, high energy compounds, coupled

reactions,determination of feasibility of reactions, Atkinson’s energy charge,

phosphorylation potential and

3 Membrane Transport

Aerobic and anaerobic respirationmitochondrial electron transport chain,

structure and function of ATPase, generation and maintenance of proton

motive force,oxidative phosphorylation, inhibitors and un

electrontransport chain and oxidative phosphorylation.

Concept of anaerobic respiration, components of electrontransfer system and

energy generation of bacteria where nitrate,sulfate and carbonate acts as

terminal electron acceptors

4 Nitrogen metabolism

Biochemistry of biological nitrogen fixation, properties of nitrogenase and

its regulation, ammonia assimilation with respect to glutamine synthetase,

glutamate dehydrogenase, glutamate synthetase, their properties and

regulation,Biosynthesis of

Biosynthesis of purine and pyrimidine bases

Photosynthesis

Structure of chloroplast, energy consideration in photosynthesis, light and

dark reaction, electron carriers in photosynthesis, Organization of

photosystem I and II, cyclic and non

reaction, photolysis of water, C

Regulation of photosynthesis, Bacterial photosynthesis: scope, electron

carriers, Photosynthetic reaction center, cycl

photophosphorylation in various groups of phototrophic bacteria, electron

donors other than water in anoxygenic photosynthetic bacteria

5 Biosynthesis of carbohydrates in plants and bacteria

Calvin cycle and its

membrane, Synthesis of starch and sucrose,Photorespiration, C

pathways, synthesis of celluloseand peptidoglycan, integration of

carbohydrate metabolism inplant cell.

Lipid Biosynthesis

Synthesis of storage lipids: Fatty acids and triacylglycerols,Synthesis of

membrane lipids: Glycerophospholipids, sphingolipids, sterols, Lipids as

signal molecules such asphosphatidyl inositol, eicosanoids, Vitamins, A, D,

K, and E,Dolichols.

Course Outcome


CO1 Student will be able to understand the concepts of microbial physiology

CO2 Student will be able to understand the metabolic processes carried out in microorganisms


standardconditions, high energy compounds, coupled


phosphorylation potential and its significance

Membrane Transport



motive force,oxidative phosphorylation, inhibitors and un-couplers

electrontransport chain and oxidative phosphorylation.



terminal electron acceptors

metabolism




regulation,Biosynthesis of five families of amino acids and histidine,

Biosynthesis of purine and pyrimidine bases



tem I and II, cyclic and non-cyclic flow of electrons, Z scheme, Hill

reaction, photolysis of water, C3, C4 CAM plants, Photorespiration,


carriers, Photosynthetic reaction center, cyclic flow of electrons, bacterial


donors other than water in anoxygenic photosynthetic bacteria

Biosynthesis of carbohydrates in plants and bacteria

Calvin cycle and its regulation, Transport of solute acrosschloroplast

membrane, Synthesis of starch and sucrose,Photorespiration, C4 and CAM


carbohydrate metabolism inplant cell.

storage lipids: Fatty acids and triacylglycerols,Synthesis of



understand the concepts of microbial physiology

understand the metabolic processes carried out in microorganisms


standardconditions, high energy compounds, coupled




couplers of



12 L




five families of amino acids and histidine,



cyclic flow of electrons, Z scheme, Hill

CAM plants, Photorespiration,


ic flow of electrons, bacterial


12 L

regulation, Transport of solute acrosschloroplast

and CAM


storage lipids: Fatty acids and triacylglycerols,Synthesis of



12 L

Total 60

understand the metabolic processes carried out in microorganisms


microbial metabolism


enzymes from biological systems

CO5 Student will be able to integrate

Resources

Recommended

Books

1. Cox M. M., Nelson D. L., (2008) Lehninger Principles ofBiochemistry,

Fifth edition, W. H. Frreman and CompanyNew York

2. Garrett, R. H. and Grisham, C. M. (2004)

Brooks/Cole, Publishing Company, California.

3. Hall D. D. and Rao K. K. (1996)

University Press

of Bacterial Growth

4. Michael T. Madigan, John M. Martinko, David A. Stahl,David P. Clark

(2012) Brock Biology of Microorganisms

Cummings, San Francisco.

5. Moat Albert G. and Foster John W. (1988)

John Wileyand Sons New York.

Reference Books

6. Nelson D. L. and Cox M. M. (2005)

Fourth edition, W. H. Freeman & Co. NewYork.

7. Palmer Trevor (2001)

chemistry, Horwood Pub.

8. White David (2000)

Oxford University Press, New York.

Student will be able to understand the role of enzymes in biological systems including

Student will be able to study enzyme kinetics, extraction and purification methods of

enzymes from biological systems

to integrate the fundamental processes with their applications

Cox M. M., Nelson D. L., (2008) Lehninger Principles ofBiochemistry,

Fifth edition, W. H. Frreman and CompanyNew York

Garrett, R. H. and Grisham, C. M. (2004) Biochemistry.

Brooks/Cole, Publishing Company, California.

Hall D. D. and Rao K. K. (1996) Photosynthesis 5th Ed.,Cambridge

University PressMandelstam Joel and McQuillen Kenneth (1976)

of Bacterial Growth, Blackwell ScientificPublication London.

Michael T. Madigan, John M. Martinko, David A. Stahl,David P. Clark

Brock Biology of Microorganisms,Thirteenth edition, Benjamin

Cummings, San Francisco.

Moat Albert G. and Foster John W. (1988) MicrobialPhysiology

yand Sons New York.

Nelson D. L. and Cox M. M. (2005) Lehninger’s Principles ofBiochemistry

Fourth edition, W. H. Freeman & Co. NewYork.

Palmer Trevor (2001) Enzymes: Biochemistry, Biotechnologyand Clinical

, Horwood Pub. Co. Chinchester,England.

White David (2000) Physiology and Biochemistry ofProkaryotes.

Oxford University Press, New York.

understand the role of enzymes in biological systems including

study enzyme kinetics, extraction and purification methods of

the fundamental processes with their applications

Cox M. M., Nelson D. L., (2008) Lehninger Principles ofBiochemistry,

Biochemistry. 3rd

Ed.

5th Ed.,Cambridge

Mandelstam Joel and McQuillen Kenneth (1976)Biochemistry

ion London.

Michael T. Madigan, John M. Martinko, David A. Stahl,David P. Clark

,Thirteenth edition, Benjamin

MicrobialPhysiology2nd Ed.

Lehninger’s Principles ofBiochemistry,

Enzymes: Biochemistry, Biotechnologyand Clinical

Physiology and Biochemistry ofProkaryotes. 2nd Ed.

Year: First Year

Course: Microbial Diversity and Taxonomy A

and Molecular Biophysics Laboratory

Teaching

Scheme

(Hrs/Week)


L T P C CIA-1 CIA-2

0 0 4 2 - -


Objectives

1 To acquire proficiency in identification of mi

2 To train the students in advance microbial techniques.

3 To impart the knowledge of analytical methods in biological sciences

Sr.

No.

1 Isolation of the following types of bacteria from natural sampl

Identification of the bacteria to at least the Genus level using the Bergey’s Manuals:

The identification key must be designed for each isolated and identified bacterium. 2 Isolation of the following types of fungi from natural samples.

Identification of the fungi. Molds (Saprophytic); Yeasts

The identification key must be designed for each isolated and identified fungus. 3 Isolation and identification of any one type of cyanobacterium from a natural sample.

The identification key must be designed4 Preparation of buffers.

5 Chromatography: separation of sugar and amino acids by paper and thin layer

chromatography. 6 Colorimetry and spectrophotometry: Estimation of sugar and total carbohydrates,

estimation of protein by lowry. Bradford and UV spectrophotometry. 7 Electrophoresis: Agarose gel electrophoresis,

8 Electrophoresis: SDS PAGE of proteins.

Term Work:

Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Ter

continuous assessment based on

journal/record book, oral/viva, respectively

At the end of the semester, the final grade for

performance of the student and is to be submitted to the University.

School of Science

M.Sc. Microbiology

Semester: I

ial Diversity and Taxonomy And Instrumentation

Laboratory

Course Code:


Examination


- - 50 - 50


To acquire proficiency in identification of microorganisms using Bergey’s manual

To train the students in advance microbial techniques.

To impart the knowledge of analytical methods in biological sciences.

Description

Isolation of the following types of bacteria from natural samples.


The identification key must be designed for each isolated and identified bacterium.

Isolation of the following types of fungi from natural samples.

ion of the fungi. Molds (Saprophytic); Yeasts

The identification key must be designed for each isolated and identified fungus.

Isolation and identification of any one type of cyanobacterium from a natural sample.

The identification key must be designed for each isolated and identified cyanobacterium.

Chromatography: separation of sugar and amino acids by paper and thin layer

Colorimetry and spectrophotometry: Estimation of sugar and total carbohydrates,

estimation of protein by lowry. Bradford and UV spectrophotometry.

Electrophoresis: Agarose gel electrophoresis,

Electrophoresis: SDS PAGE of proteins.

Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Ter

continuous assessment based on attendance, good laboratory practice (GPL), timely completion,

journal/record book, oral/viva, respectively. It should be assessed by course teacher of the institute.

At the end of the semester, the final grade for a Term Work shall be assigned based on the

performance of the student and is to be submitted to the University.

Semester: I

Course Code: PMI111

Total

100

croorganisms using Bergey’s manual.


The identification key must be designed for each isolated and identified bacterium.

The identification key must be designed for each isolated and identified fungus.

Isolation and identification of any one type of cyanobacterium from a natural sample.

for each isolated and identified cyanobacterium.

Chromatography: separation of sugar and amino acids by paper and thin layer

Colorimetry and spectrophotometry: Estimation of sugar and total carbohydrates,

Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Term work is

attendance, good laboratory practice (GPL), timely completion,

teacher of the institute.

a Term Work shall be assigned based on the

Notes

1 The regular attendance of the

marks will be given accordingly (

2 Good Laboratory Practices (

3 Timely Completion (10 Marks)

4 Journal / Record Book (10 Marks)

5 Oral / Viva (10 Marks)

Practical/Oral/Presentation:

Practical/Oral/Presentation shall be conducted and assessed jointly by at least

appointed as internal and external

mark/grade sheet in the format as specified by the University, authenticate and seal it. Sealed envelope

shall be submitted to the head of th

Notes

1 One experiment from the regular practical syllabus will be conducted (40 Marks).

2 Oral/Viva-voce (10 Marks).

the students during semester for practical course will be monitored and

rks will be given accordingly (10 Marks).

Good Laboratory Practices (10 Marks)

Timely Completion (10 Marks)

Journal / Record Book (10 Marks)

Practical/Oral/Presentation shall be conducted and assessed jointly by at least

internal and external examiners by the University. The examiners will prepare the


shall be submitted to the head of the department or authorized person.

One experiment from the regular practical syllabus will be conducted (40 Marks).

practical course will be monitored and

Practical/Oral/Presentation shall be conducted and assessed jointly by at least a pair of examiners

The examiners will prepare the



Year: First Year

Course: Cell and Molecular Biology and Enzyme & Microbial

Metabolism Laboratory

Teaching

Scheme

(Hrs/Week)


L T P C CIA-1 CIA-2

0 0 4 2 - -


Objectives

1 To understand various cell activities using microscopic techniques.

2 To create the general understanding among students related to production of bio

3 To give the overview for development of scientific methods of screening, characterizing

microbes and their use in production of microbial products.

Sr. No.

1 Studying the stages mitosis in growing tip of onion root cells

2 Demonstration of mounting of embryo

stages on permanent slides

3 Isolation and characterization of bacterial pigment

4 Isolation and estimation of chromosomal DNA of bacteria

5 Isolation and characterization of (as nitrogen fixers) Rhizob

6 Detection of siderophore production by

7 Production and extraction of bacterial enzyme. Purification of enzyme by ammonium

sulphate precipitation and dialysis.

8 Isolation and characterization of biodeg

Term Work:


continuous assessment based on Attendance, G

Journal/Record book and Oral. It

the semester, the final grade for a Term Work shall be assigned based on the performance of the

student and is to be submitted to the University.

Notes

1 The regular attendance of the

marks will be given accordingly (10

School of Science

M.Sc. Microbiology

Semester: I

and Molecular Biology and Enzyme & Microbial Course Code:


Examination


- - 50 - 50


To understand various cell activities using microscopic techniques.

To create the general understanding among students related to production of bio

for development of scientific methods of screening, characterizing

microbes and their use in production of microbial products.

Description

Studying the stages mitosis in growing tip of onion root cells.

Demonstration of mounting of embryos (frog and fruit fly) at various developmental

stages on permanent slides.

Isolation and characterization of bacterial pigment.

Isolation and estimation of chromosomal DNA of bacteria.

Isolation and characterization of (as nitrogen fixers) Rhizobium or

Detection of siderophore production by Azospirillum and Pseudomonas

Production and extraction of bacterial enzyme. Purification of enzyme by ammonium

sulphate precipitation and dialysis.

Isolation and characterization of biodegradable bacteria.


based on Attendance, Good Laboratory Practice (GLP)

Journal/Record book and Oral. It should be assessed by subject teacher of the institute. At the end of


student and is to be submitted to the University.

the students during semester for practical course will be monitored and

rks will be given accordingly (10 Marks).

Semester: I

Course Code: PMI112

Total

100

To create the general understanding among students related to production of bio-products.

for development of scientific methods of screening, characterizing

s (frog and fruit fly) at various developmental

ium or Azospirillum

Pseudomonas

Production and extraction of bacterial enzyme. Purification of enzyme by ammonium


(GLP), Timely Completion,

d be assessed by subject teacher of the institute. At the end of


practical course will be monitored and

2 Good Laboratory Practices (

3 Timely Completion (10 Marks)

4 Journal / Record Book (10 Marks)

5 Oral / Viva (10 Marks)

Practical/Oral/Presentation:


appointed as internal and external



Notes

1 One experiment from the regular practical syllabus will be conducted (40 Marks).

2 Oral/Viva-voce (10 Marks).

Good Laboratory Practices (10 Marks)

Timely Completion (10 Marks)

Journal / Record Book (10 Marks)


internal and external examiners by the University. The examiners will prepare the

as specified by the University, authenticate and seal it. Sealed envelope




The examiners will prepare the

as specified by the University, authenticate and seal it. Sealed envelope


year: first year semester: i course: microbial diversity and ......year: first year course:...

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