YOU BREAK IT YOU BUY IT

The MicroscopeParts of, Use of, and Care

The Compound Microscope

1. Is used to make the small, fine details of objects visible to the human eye.

The First Microscope

2. Was made by Anton van Leeuwenhoek.

The First Compound Microscope

3. Was made by Robert Hooke.

The Basic Microscope

Used the reflection of light off mirrors as a light source.

Now LED light source is used…

The Modern Day Microscope

Has come a long way in the last 300 years with new technology.

Electron Microscopes

5. Instead of light, electron microscopes beam electrons at the specimen and get a picture with greater magnification than possible with a light microscope

Pollen

Fly’s Foot

Ant

“LEARNING THE PARTS OF A MICROSCOPE IS LIKE LEARNING ABOUT THE PARTS OF A CAR.”

The Parts of a Microscope

Eye piece Body Tube Arm Nosepiece Stage ClipsObjectives Stage Stop Aperture StageCoarse Adjustment Knob DiaphragmLight SourceFine Adjustment Knob Power Switch Base

Quiz

“LEARNING HOW TO USE A MICROSCOPE IS LIKE LEARNING HOW TO DRIVE A CAR.”

The Use of a Microscope

Proper Use of Microscope

1. When moving your microscope, always carry it with two hands. Grasp the arm with one hand and place your other hand under the base for support.

 

2. Turn the nosepiece so that the lowest power objective lens is "clicked" into position and make sure stage is lowered.

3. Place a microscope slide onto the stage and fasten it with the stage clips.  Carefully move the specimen to the center of the stage (above the aperture).

4. Look through the eyepiece and adjust the light intensity by turning the diaphragm to allow the greatest amount of light to shine through.

Proper Use of Microscope

5. Looking from the side slowly turn the coarse adjustment knob so that the stage comes up almost to the lens. (DO NOT ALLOW THE MICROSCOPE LENS TO TOUCH THE SLIDE!)

6. Use the coarse adjustment knob to focus the image as you move the stage down and away from the lens. Stop when the image is clear.

Proper Use of Microscope

7. Readjust the microscope slide so that the image is in the center of the aperture and readjust the diaphragm for the clearest image.

8. Change to the next objective lens by “clicking” it into position. Use ONLY the fine adjustment knob to bring it back into focus.

Proper Use of Microscope

9. Switch to the next objective lens by “clicking” it into position. Again ONLY use the fine adjustment knob to focus.

10. When finished, lower the stage, “click” the lowest objective lens into position, remove the slide, wrap up the power cord, and carry it safely to the shelf.

Proper Use of Microscope

.”“YOU BREAK IT, YOU BUY IT!”(THESE ARE EXPENSIVE TOOLS.)

The Care for a Microscope

Microscope Rules

1. Set the microscope at least 4 inches from the edge of the desk or table.

2. Check over the microscope before you start to use it (report any problems to the teacher).

3. Plug-in the cord if needed and keep it out of your way.

Microscope Rules

4. Do not lean on the desk or table and do not make any sudden movements.

5. Be careful with all microscope slides, they are made of glass and can harm you.

6. Let me know if anything happens.

“SOME HELPFUL AND INTERESTING TIPS.”

The Workings of a Microscope

The Lenses

1. Are used for magnification (enlargement of the object) and resolution (making the object clearer).

Magnification of Objects

To determine how much larger an object appears:

(Multiply the eyepiece lens times objective lens.)

Eyepiece x Objective = Total Magnification

Magnification of Objects

1. Eyepiece = 10X

2. Objectives:Low power = 4XMedium power = 10XHigh power = 40X

Sample Problem

1. If the ocular is 10X and the objective is 43X, what is the total magnification?

(Eyepiece 10X) X (Objective 43X) = ______430

Resolution of ObjectsResolution of Objects1. Objects always appear inverted and reversed.

(upside down and backwards)

* If an “e” is placed in the stage in its normal position it will appear…

2. Only a thin layer of the object can be in focus at one time. (depth of focus)

Resolution of ObjectsResolution of Objects

Low power medium power high power

a. Size of the field of view decreases

b. Field of view is darker

c. Size of the image increases

d. Resolution increases

e. Working distance decreases

Non-Living Specimens

Resolution of ObjectsResolution of Objects

Living Specimens

Actual movement is opposite to appeared direction of movement. (If it looks like its going to the Right it’s really going to the Left.

Similarly if it appears to move Up it’s actually moving Down.

Any Questions?