2011 lab 1 enzymology.pdf

8/14/2019 2011 Lab 1 Enzymology.pdf

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LABORATORY 1

LABORATORY GUIDE BIOSCI 101

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LABORATORY 1

Enzymology

Enzymes are proteins which catalyse chemical reactions and they enable these reactions to proceed

rapidly at the comparatively low temperatures of living cells.

In this practical session you will use a prepared homogenate of potato which contains many

enzymes. One enzyme in particular will be chosen to enable you to study its:

rate of reaction

sensitivity to heat

substrate specicity

requirement for a co-factor

Suggested readings in Campbell: 9th edn Chapter 8, pp. 198-206; 8th edn Chapter 8, pp. 151-159

In the following experiments you will examine some of the properties of a plant enzyme called

polyphenol oxidase. This enzyme catalyses the following reaction:

Work in pairs

Part 1 Practise measuring and dispensing volumes using a micropipette

(1 1/2 hours)

1.2 Rate of reaction

1.3 Denaturation 1.4 Substrate 1.5 Cofactor requirements

specifcity

Part 2 Undergraduate Computer Laboratory Session in B14

(1 1/2 hours)

Preparation for Laboratory 2

NOTE: Some lab classes will start with the undergraduate computer laboratory session.

Follow instructions given by your tutor.

Flow chart for Laboratory 1

(Source: Vivian Ward, SBS)


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1.4 LABORATORY 1

BIOSCI 101 LABORATORY GUIDE

Use of a micropipette

Operation: Place a tip on the shaft of the pipette. Press the tip on rmly using a slight twisting

motion to ensure a positive, airtight seal.

Aspiration: Holding the pipette vertically, depress the pushbutton down to the FIRST STOP

and immerse the tip into the sample liquid. Release the pushbutton SLOWLY and smoothly

to aspirate the sample. Wait one second and then withdraw the tip from the liquid.

Dispensing: Place the end of the tip against the inside wall of the vessel at an angle of 10-

40 degrees. Push the pushbutton SLOWLY to the rst stop. Wait one second. Press the

pushbutton to the SECOND STOP to expel any remaining liquid. Keeping the pushbuttonpressed to the end, remove the pipette by drawing the tip along the inside surface of the

vessel. Release the pushbutton.

Using an automatic pipette to measure and

transfer volumes of liquid

Please Note: Each pipettor costs around $500. Please handle them carefully, and

avoid dropping them etc.

Exercise: (work individually)

Using the appropriate pipettor, pipette the following volumes of liquid onto aspotting tile.

Dye: 10 l of red dye 15 l green dye 20 l yellow dye

Water: 0.5 ml (500 l) 1.0 ml (1000 l) 4.0 ml (4000 l)

UNITS

MASS VOLUME

g mg g L mL L

CONVERSIONS

1 g = 1,000 mg =

1,000,000 g

1L = 1,000 mL =

1,000,000 L

0.000001 g =

0.001 mg = 1 g

0.000001 L =

0.001 mL = 1 L

NOTE: Incorrect use of units has resultedin the failure of many experiments, makesure you understand the differences in scalerepresented in the table above.

Examples: for 0.5 mL ... use a p1000 for 2 mL .......use a p5000 for 10 L ........use a p20 for 50 L use a p100

Pipettor p20 p100 p1000 p5000

Maximumvolume

measured20 L/

0.02 mL100 L/0.1 mL

1000 L/ 1 mL

5000 L/5 mL

Maximum

dialsetting

2

0

1

00

1

000

5

000


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LABORATORY 1


1.5

The rate of reaction in enzyme experiments can be affected by temperature, pH and ionic

strength, which all need to be kept constant.

Control experimentsare a necessary part of the protocol in all biological experiments.

Controls enable the operator to discern if the effects observed are real or artifacts. Anexample of such artifacts involving enzymes could be contamination of the enzyme

preparation or reactions mediated by substances in the reaction mixture other than the

enzyme. Control experiments are set up by substituting heat inactivated enzyme samples for

active ones, or preparing reaction mixtures without one of the essential components.

Enzymes

Kinetic experiments may be used to study the effect on the reaction of factors such as

substrate concentration, enzyme concentration, physical and chemical modiers of

enzyme activity, involvement of intermediate complexes and structural or conformational

changes which may take place in the enzyme itself. For kinetic studies the enzyme andsubstrate are mixed and the substrate or product concentration is recorded as a function

of time. A typical progress curve is shown below.

Fig. 1

The maximum rateof the reaction is on a tangent to the steepest region of the curve

passing through to. The maximum rate is known as the initial velocityof the reaction.

Progress curve for the enzyme catalysed reaction S P

(Source: Vivian Ward, SBS)


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1.1 Potato: Preparation of homogenate

The technician has peeled a potato, washed it

in deionised water and sliced it into a solution

of phosphate-buffered saline (PBS) at pH

6. Enzymes are usually prepared in buffered

solutions which maintain the pH at a constant

value. The PBS also contains sodium uoride

(NaF) which prevents the oxidation of variousnaturally occurring compounds in the potato

by inhibiting the enzymes responsible for

oxidation: it does not inhibit polyphenol

oxidase.

The sliced potato is homogenised in cold buffer

in a blender and the homogenate ltered to

remove incompletely homogenised debris.

The extract is diluted with three volumes of

buffer.

Each group has been given 20ml of this extract.

Place at 0oC in the ice bucket provided.

Note the colour of the extract given toyou.

Colour ofextract: ______________________

Note: There are three important technical points to observe when carrying out these

experiments.

1. Allow adequate time for temperature equilibration (3 min) when you remove reagents

from the ice bath, before initiating a reaction.

2. During the course of the enzymatic reaction, test-tubes must be shaken every 30

seconds to ensure adequate aeration (oxygen is one of the substrates).

3. WARNING! Sodium uoride (NaF), catechol and phenol are all poisonous. Phenol

especially must be handled with a maximum of care since splashes to the skin cause

serious burns which can be fatal. Use water to remove phenol from skin. Wash down

with cold water if spilled on bench.

Polyphenol oxidase

We will study the polyphenol oxidase in a

crude extract of potato. The crude extract

contains many other enzymes. One of

these appears to convert benzoquinone to

highly coloured reddish compounds. Thus

benzoquinone is an intermediate, not a

stable product, in this system. While this

complication makes it difcult to obtain

quantitative data, it does represent a

common situation. Most reactions in the

cell are part of a long chain of reactions (a

metabolic pathway) where the product of

one reaction is the substrate for a further

enzyme. In this way a compound can be

converted into a seemingly unrelated species

with quite a different structure through a

series of small modications. For the purposeof this class experiment, the side reactions

have been minimised by carrying out the

reaction at pH6.

In order to study the properties of our

enzyme more adequately its purication

would be necessary (this task is well beyond

our scope). Once puried, the structure of

polyphenol oxidase could be studied, and

its catalytic properties could be examinedwithout the complicating effects of the

subsequent metabolism of benzoquinone.


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LABORATORY 1


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1.2 Rate of reaction

This experiment will establish the timing for all the experiments you will perform this

morning.

CAUTION: Use a pipettor for all enzyme additions (with tip).

Label three test-tubes A, B and C and add the following

Immediately following the addition of the second component, shake tube and continueto do so every 30 seconds. Watch carefully for the appearance of a yellow colour.Ideally the colour will start appearing after 15-20 seconds and will be a strong yel-low (as distinct from gold) after 2-3 minutes, but the enzyme activity varies greatlywith the age of the potato. Record the time taken to produce a strong yellow colour.Check with a demonstrator if it takes less than 2 minutes. In the table below identify

(tick) what you placed in each tube.

RESULTS (to be used in future readings):

Note: Tubes B and C are controls. What purpose do they serve?

Tube B ________________________________________________________

Tube C ________________________________________________________

Tube A Tube B Tube C

Enzyme Extract 1 mL (1000 l) 1 mL - Buffer (PBS/NaF) - - 1 mL

Tube A Tube B Tube C Catechol (1 molL-1) 50 l - 50 l Buffer (PBS/NaF) - 50 l -


Enzyme ExtractSubstrate

Buffer


Appearance ofYellow Colour (Yes/No)

Time Taken

After these solutions have come to room temperature, make the followingadditions using an automatic pipette:


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1.3 Denaturation of polyphenol oxidase

Test the heat stability of polyphenol oxidase by pipetting 1ml (1000 l) of enzymeextract into each of two test-tubes. Heat one in a boiling water bath for twominutes, then allow it to cool to room temperature. Keep the other test-tube at

room temperature throughout the experiment (control). Then add 50l of 1 mol.l-1

catechol to both tubes and observe whether a reaction occurs.

RESULTS____________________________________________________________________

_____________________________________________________________________________

1.4 Substrate specicity of polyphenol oxidase

While some enzymes are absolutely specic for one substrate, others are capable of catalysing

reactions using several closely related substrates.

This experiment will test the specicity of polyphenol oxidase by using three polyphenols

(phenol, catechol and hydroquinone) as substrates. Each of these closely relately compounds

forms coloured products when oxidised.

Add the following to new, clean test tubes labelled A, B and C:


Enzyme 1 ml 1 ml 1 ml

Phenol 50l - -

Catechol - 50l -

Hydroquinone - - 50l

Note whether any reaction occurs with each substrate.


Substrate

Reaction(Yes / No)


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1.5 Cofactor requirements of polyphenol oxidase

Many enzymes require other small molecules (cofactors) in order to catalyse reactions. Copper

ions are cofactors for certain enzymes. We will test whether polyphenol oxidase has such a

requirement by adding phenylthiourea to the reaction mixture. This organic compound has

the ability to form complexes with copper ions.

Pipette 1ml (1000l) of enzyme extract into each of two test-tubes: - add 250l of the saturated phenylthiourea solution to one tube - add 250l of water to the other tube (control)Shake the tubes to ensure good mixing. Now add 50l of 1 mol. l-1catechol to eachand observe whether a reaction occurs.

Results: _____________________________________________________________________

Preparation for Laboratory 2

During this practical streak your three unknown strains of E. colionto the plates provided. The

procedure will be explained and supervised by a demonstrator and is outlined in 2.4, Laboratory

2. The plates will be incubated and returned for analysis during your next laboratory.

WASH YOUR HANDS WELL BEFORE LEAVING THE LABORATORY

Computer Laboratory Session

The Tutor-in-charge of your stream will indicate when your class can proceed to the Under-

graduate Computer Laboratory, Room B14 in the basement of this building.

During this session you will learn about the following:

DELNA and complete the assessment

how to use SBS computers and use NetLogin

nding your way around the electronic campus including using your email

how to use the learning management system CecilTM as a tool to support your

learning.

Virtual experiment. Use an interactive animation investigating enzyme (polyphenol

oxidase) activity at different pH levels.

Exercises will be available in CecilTM during this session and together with your tasks on the

assignment sheet are the assessment for this laboratory.


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Enzymology Hazardous Substances

Chemical Formula Hazard Additional Information

Catechol C6H6O2 Corrosive/Harmful Readily absorbed throughskin. Target organs- liver/

kidneys. If inhaled can

be very harmful to the

respiratory system.

Sodium Fluoride NaF Toxic Very Toxic. Ingestion can

be fatal. Do not inhale or

touch skin.

Sodium Chloride NaCl

Di-SodiumHydrogen

Phosphate

Na2HPO4 May be harmful i fswallowed.

Sodium di-

Hydrogen

Phosphate

NaH2PO

4Irritant Eye and skin irritant

Hydroquinone C6H

6O

2Toxic May cause cancer. Skin and

respiratory irritant.

Phenol C6H

5OH Poison Acute poisoning will

result from swallowing orinhalation. Causes severe

burns. Skin contact may

lead to death.

Phenylthiourea C7H

8N

2S Toxic Fatal if swallowed. Eye

and skin irritant.

CLEANING UP INSTRUCTIONS

1. Place a) colour coded test tubes

b) colour coded pasteur pipettes c) white tiles d) boiling tubes(glass test tube with red label around rim)

e) potato extract ask f) white/blue/yellow pipette tips

in labelled containers by sink.

2. Place glass test tubes (not the test tube rack!) in bin on trolley by sink.3. EMPTY ice bucket and stack on side bench.4. Stack white tray on side bench.

Tip all liquids down sink.Do not rinse any glassware.

WASH YOUR HANDS


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Computer Laboratory Session

1. DELNA

In the rst part of this session you will complete the DELNA (Diagnostic English Language

Needs Assessment). More information about this assessment is on the next page.

To begin the assesment, follow the tutor directions. When you nished, request one of the

demonstrators log your computer out of the secure website. Then proceed to the next part,

"Learning Technologies" and complete at your own pace.

2. Learning Technologies.

Complete the following tasks using the "Guide to the Undergraduate Computer Laboratory"

log into your computer and then log into NetLogin

log into Cecil.

read announcements.

nd where your marks will be displayed

view powerpoints

view animations

attempt Quiz 1

visit the discussion forum and make a posting

complete survey

vote for student representation

log into MasteringBiology and activate your access code if not already done

this


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DELNA

Diagnostic English Language Needs Assessment

English for university study

All university students need to develop their academic writing skills. Many students will do

this through extensive reading and listening but others may need to attend writing work-

shops or take other forms of instruction. In order to help those who may need to work on

their academic English language skills as they begin their studies the University has students

take DELNA.

All rst-year students will do the DELNA screening assessment at the beginning of their rst

semester. This is a computer-based assessment made up of two tasks: vocabulary and rapidtext-editing and will take no more than 30 minutes.

If your screening results suggest that you may need some assistance with the demands of

academic English you will then be asked to do the DELNA diagnosis. This assessment gives

you a great opportunity to have important areas of communication assessed: reading, listen-

ing and writing. It takes 2 hours, it is free and only for enrolled students at UoA. Your results

are for your guidance only and do not appear on any academic record.

You will be given advice about which courses, tutorials, workshops, or self access courses you

could take to increase your chances of academic success and get you off to a ying start withyour studies.

You do the screening once only. Once you have done it we let all your First-Year Course Coor-

dinator know.

For examples of the assessment components go to: www.delna.auckland.ac.nz

During the computer session you will complete the DELNA. This conveniently ena-bles you to complete the assessment early in the semester and benet from anyfollow up support if required.


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Guide to the Undergraduate Computer Laboratory

There are a number of computers available for undergraduate students to use in the School of Biological

Sciences, mostly MS Windows compatible PCs machines. These include approximately 80 PC computers

in the Undergraduate Computer Lab (UCL) Room B14. During semesters, including mid semester break

and study break prior to exams, the lab is open from 9-5 pm weekdays and help is available in the lab

if you have any problems or queries related to using the computers. As this lab is booked sometimes

for teaching, it is recommended to check the whiteboard by the entrance for current teaching bookings

& hours. Additionally, you can use the Kate Edger Information Commons where 500+ computers are

available.

The topics covered in this section are:

1. Logging in to the SBS computers.

2. Logging in to NetLogin.

3. Printing at the university

4. Saving Work and AFS5. Electronic Campus (EC) and starting a web browser.

6. Using your Student Email Account

7. Logging out of the Computer

1. Logging in to the SBS computersTo use the computers you must rst log in. Press CTRL-ALT-DEL.

This should bring up the login page. Type in your UPI (also known

as a NetID) and NetPassword (this is the same as you nDeva login

information). Left click on the OK button. Left click on the OK button

for any further messages.

2. NetLogin

The University operates Internet access, email and on campus printing

services for all students. These services are collectively known as

NetAccount services, and provide: access to the Internet from within the University; access to University

resources from outside the University; printing and photocopying on campus (both charged separately

from internet charges).

To use NetAccount you need to log in to NetLogin using your NetLogin

username and password (usually this happens automatically, but

in some cases you may have to log in manually by opening the Net

Login.exe programme). Your username identies you as a student of the

University of Auckland and can be referred to as your NetLoginID, NetID,

User ID, or UPI.

To change your NetPassword: Left click on NetLogin, then left click on 'Change Password' and

follow the instructions. DON'T change your password in the rst week as this often causes problems.

If you are enrolled in a course at the University access to the Internet is free by logging on

to NetLogin. This connection - the Undergraduate Plan - provides access to University web

resources and online resources provided by the Library. You also get high-speed access to all

non-University websites with a 200 MB monthly data allowance. Additional data amounts


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1.14 LABORATORY 1


can be purchased if you reach your 200 MB monthly limit (100 MB is $2).

More info: http://ec.auckland.ac.nz/docs/net-student.htm.

3. Printing at the University

For printing and photocopying you will need to add money (credit) to your netAccount Printing Account.

There are three different ways to add credit to your NetAccount:

1. Using an Autoloader (cash only);

2. ePOS (self-service; EFTPOS only);

3. At the IC Helpdesk (Level 2, Kate Edger IC, Grafton IC or Epsom IC) or other NetAccount service

points (EFTPOS & credit card only).

More info: http://ec.auckland.ac.nz/docs/addmoney.htm.

You can transfer credit you have

purchased between your accounts at:

https://admin.ec.auckland.ac.nz/

Account/transferQuota.cgi)

To print:

1. Make sure you are logged in to NetLogin.

2. Send a job from the application you are

running to be printed (in the Biology

Undergraduate Computer Lab choose

'SBS NetAccount Printer' for black and

white or SBS Colour NetAccount Printer

for colour).

3. Swipe your card through any of the

NetPrinter computer stations (also

known as EC print stations) located in

the Biology Building (Room B14) or login with the keyboard.

4. Choose the document you wish to be printed. The document will then print from that printer and

be debited against your NetLogin Account (please be patient as sometimes it will take time to get

through the network).

You can also print double sided or print multiple pages per sheet, to nd out more about this ask at the

help desk in the computer lab.

4. Saving work

The University provides 250MB of network le storage space to all enrolled students to store their les

and documents. This storage space is available from within most computers labs run by Science, from

the Information Commons, and over the Internet. It is recommended you always have a second copy

of your work and save your les regularly.

In the Biology Undergraduate Computer Lab the space is available from the desktop (as the User Home

Directory icon) as long as you are logged in to the AFS client (see AFS below). The table lists the commonaccess links for you storage space in different labs around the campus.


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Computer Lab Science Storage Location

Information Commons My Documents

Biological Sciences/ CompSci / Maths /

Stats

User Home Directory icon on the

desktop or H: drive under My Computer

Anywhere else on campus or on the web

(including the Biology UCL)

Point your web browser at

https://afsweb.ec.auckland.ac.nz

AFS client login:

To access your User Home Directory you need to have logged in to what is known as the AFS client.

Again, this login is your NetID and Net password. You may be automatically logged in to AFS or a login

screen may appear when you rst log in to the computers. If it does not you can click on the little key

located in the bottom right of the screen and then click on 'Obtain

New Tokens' where you will then be able to enter your details.

5. Electronic Campus

The Electronic Campus (EC) provides computer services for students such as electronic

mail (email), internet browsing and electronic libraries. It is delivered via the Internet,

therefore to access it you need to open a web browser such as Internet Explorer or

Mozilla Firefox. To do this left click twice on the icon called Internet Explorer on the Desktop. This

will start up the browser. Make sure you are logged in to NetLogin before you open up the browser if

you want to go out into the internet.

In the address bar type in the address: http://www.auckland.ac.nz. This will bring you to the universitys

home pages. Put the cursor above the Current student link on the left and click. The current student

page is where you will nd resources available to you as a student (and lots of information). All Internet

access and email within the university is free (i.e. with the address auckland.ac.nz). Everything else will

be charged to your NetLogin Account. For current NetLogin internet access and email rates check with

the IC helpdesk in the Kate Edger Information Commons or look under the word "help" in NetLogin.

6. Using your student Email

The University provides enrolled students with an email address ([email protected]) and a

mailbox with 6GB+ hosted by Google. The email address is your NetID characters with the addition

of @aucklanduni.ac.nz, for example: [email protected].

The University will use this email address to communicate with you. Even if you have your own email

address, you must check your new University EC email inbox, as this address is often used by lecturers,

tutors, administrators and the Library to contact you. The address for email is: https://webmail.

ec.auckland.ac.nz/webmail.html.

7. Log out of the computer.

Shut down all of your windows. Press Ctl-Alt-Del. Choose log off. Left click on the Yes button.It is important to log out of the computer when you have nished to avoid accidentally leaving your

account information active for others to potentially use.


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Using Computers to access information and learning resources

1. What is Cecil and Logging in from EC

Cecil is the learning management system used within the university to provide

students with access to specic course resources such as on-line tests, lecture

resources, course discussion groups, and course marks. To access Cecil leftclick on the words Cecil in the quicklinks of the main university homepage.

This will bring you to the main Cecil page. Alternatively you can type in the address: http://www.cecil.

auckland.ac.nz. Then you just enter your information (UPI and password) and left click on the word

Login.

2. Cecil nding your way around.

There are several key areas in Cecil. When you have logged in, you will

see "What's new in you courses' and "Current Studying" in the window

on the left. If you click on the '+' beside 'Current Studying' this will open

to display the current courses you are enrolled in. To view course specicinformation you can click on a course name. You can also click on the 'All

courses" button above this at any time and it will open a list of courses

for you to select.

There are a number of main buttons at the bottom left of the page. The

information below relates to what may be seen for specic courses. Please

note - which buttons you can see will depend on what you have currently

selected ('All Courses' or a specic course) and also what has been set up

for a course.

CalendarA visual representation of activities and events for your courses. Click on the Calendar button to see the

calendar. This can be viewed in a number of ways and you can add your own personal event by clicking

on the 'New' button.

Announcements

If you left click on the Announcements button, you can access announcements, either viewing all

announcements, today's announcements or the past seven or 30 days. Whichever you choose to see,

you can read a particular announcement by left clicking on the title of that announcement and it will

appear on the right of the screen.

DiscussionTo get into a discussion forum for a particular course you need to left click on the Discussions button.

If you look at the discussion forums for biology courses, you will nd that the course coordinators have

The topics covered in this section are:

1. Cecil, logging in.

2. Cecil at a glance.

Calendar, Announcements, Discussion, Activities &Marks,Resources, Personal Settings

logging out of Cecil

3. Mastering Biology - see course information pages

4. The Bio Library and SRC Resources


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1.17 LABORATORY 1


often set up a list of topics for you to post questions and

answers into (if you like). To view a particular discussion,

click on the discussion title. You can create new posting by

click on the new button or reply to a posting by click on the reply button. You can also have all postings

to a discussion forum sent to the email address recorded in Cecil (the email address under the personal

settings) by ticking the subscribe box.

Activities & Marks

This is where you access activities and marks for activties, such as lecture information and on-line tests.

Left click on the Activities & Marks button. A list of the activities will appear on the right of the screen

near the top. For lecture resources such as lecture outlines or additional overheads you could look for

under the heading lecture and then left click on the name of the resource/le you wish to view.

Most stage 1 biology courses have online tests, which contribute to the nal mark. These online tests are

designed to help you identify the areas you need to revise. To start a test, click on Activties & Marks, click

on the activity, click on the test you wish to do and then click on the 'Start CSL Test' button (the text

on this button will change depending on which activity you are in). Sometimes

you need a password to sit a test. The person running your test session will give

this to you if necessary. When you have answered all of the questions in the

test, left click on 'Submit test'.

Resources

Under the Activities & Marks button you can access downloadable les such as lecture notes and

animations (these can also be found under the Resources button). To view or download the resource,

choose the course, click on the Activities & Marks button, click on the relevant lecture or lab; click on

the name of the le and choose to either open or save it.

Personal Settings

You can change your personal settings (e.g. your email address and preferred name) by left clicking on

the Personal Settings button.

Log out of Cecil

To log out of Cecil, left click on the Log off button near the top right, then close the web browser by left

clicking on the cross at the top right of the screen.

NB: It is important that you sit a test before the deadline. Check the end DATE and TIME for the test in Cecil

and your course guide. PLAN AHEAD and DONT RUSH IN AT THE LAST MOMENT as you cannot access the tests

after the shut off date. Most of the on-line tests you will do can be done more than once so you have a chance

to get a better understanding of the lectures if you do these reading tests before and then again after the lectures.


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3. Mastering Biology - see course information pages

4. Library Services and Resources for Biological Sciences

The SRC (Student Resources Centre) is a good rst point of inquiry for any questions you have regarding

your courses. It is also where you will hand in and pick-up most assignments for your biology courses.

Lecturers typically put information such as lecture overheads and past exams on desk copy in theResource Centre. These can be borrowed for an hour.

Biological Sciences library resources (books, journals etc.) are housed on Level M of the General Library,

5 Alfred Street, City Campus. There is a large collection of Biological Sciences library resources available

online from: http://www.library.auckland.ac.nz/subjects/bio/biosubj.htm

Finding Recommended Text Books in the Library The Short Loan Collection

The Library makes available all the recommended textbooks and required reading as well as any

additional material recommended by your lecturers in the Short Loan Collection. The Library puts the

required reading material into the Short Loan Collection. The purpose of the Short Loan collection isto make high demand items accessible to all students doing a course and when the library only has two

copies of a book the Short Loan Collection is the fairest way to do this. You can borrow items from the

Short Loan Collection for two hours at a time. The Short Loan Collection is housed on level 1 of the

Kate Edger Information Commons, on the corner of Symonds and Alfred Streets. Some readings in the

Short Loan Collection are available as on-line readings and can be viewed over the internet.

To nd Short Loan material (including on-line readings) you need to do a Course Materials Search

on Voyager (the Library Catalogue). You can access Voyager at home or in the computer labs (http://

voyager.auckland.ac.nz/) and you can also use Voyager on any computer in the Library.

To do a Course Materials Search:

1. Go to Voyager, the Library Catalogue: http://voyager.auckland.ac.nz

2. Click on the Course Material Search tab

3. From the Course drop down box select your course/paper number, eg. BIOSCI101

4. Click on the Search button

5. This screen shows you a list of all the items in the Short Loan Collection for the

paper BIOSCI101.

Under the title of each item is:

The location eg. SHORT LOAN(2 Hour) - in Kate Edger Information Commons

(Level 1), The Call Number e.g. 570 c18 2002

The Status eg. Available or On Loan - Due on 25/03/08 11:15am

6. You can only borrow Short Loan items with a status of Available. If the item you

want is On Loan this means another student is using the item, in the above exam-

ple the item is due back at 11.15am on the 25thof March. If you want an item that

is On Loan you can add your name to a waiting list for this item, ask at the desk

about

Where can I get help if I need it?

You ask for help with using the Short Loan Collection at the desk in the Short Loan Collection.

There is also an Enquiry Desk on Level 1 of the General Library and staff on this desk will

attempt to answer any question you may have.


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You can also contact John Lavas, Biological Sciences Subject Librarian, by email, phone or in

person:

email: [email protected]

phone: 373-7599 ext 87247

in person: Room M13, Level M, General Library.

Use of electronic resources and facilities is governed by a set of guidelines. Important points to

note from these guidelines include:

use of the resources for university related work

respect other users, and

respect the resources.

More detail on these points and the computer use guidelines are available in the Current Studentpages under the Technical Support - Computer Security heading or at

http://www.security.auckland.ac.nz/

Remember: The University views misuse of computers as a serious matter, and may restrict access

to its facilities even if the user is unable to complete course requirements as a result.


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