lab presentation 2011

8/3/2019 Lab Presentation 2011

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Genomics Proteomics Core

Facility

PROTEOcore


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ORGANISATION PROTEOcore

ORGANISATION PROTEOcore

Cell culture

Transgenic animal

model

DNA Sequencing

Post-genomic

Histology, cytology,

morphology

Imaging

Glassware & Lab

maintenance

Microarray

laboratory

ProteomicsLaboratory

Bioinformatics &

Biostatistics

High throughput

analytical chemistry

Translational

research

System

biology


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Research & Co-operationResearch & Co-operation

Alliance of local members of the cluster bycompetition and supply relationships orcommon interests

examples at ILS:


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RESEARCH TOPICS


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Research TopicsResearch Topics


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omics

Transcriptomics

High throughput screening

Moleculebank

Genomics / proteomics

Sclerosis Multiplex, POCD,

Alzheimer

Oral antidiabetics

Safetypharmacology

ChemiotherapyNanotechnology

Proteomic platform

Microbial genomics /proteomics / transcriptomics

Fermentation/Bioprotect

DNS animal breeding

Molecular farming

Safety pharmacology

Microarray platformBiomedical

measurement

Agro-biotechnology

Metabolomics

Enriched egg

Technology Transfer ManagementBiomedical Informatics

Joint technology platforms


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Proteomics GenomicsEven more different

Gene/ RNA

dynamic

Protein

dynamic

Stable molecules

Handling cheap/ easy

Minimal modification

Works in isolation

Fragile molecules

Handling dependent

Labile modification

Protein-interaction

Localization dependent

Handle

Tech

HTP

Sequencing (established) MS related (not yet)

DNA array / genotyping/expression / CGH/

Protein Chip (not yet)

Antibodies array (not yet)


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PROTEOcore Research &Co-operation

PROTEOcore Research &Co-operation

Indication areas at ILS

Diseases of the Central Nervous System;

Inflammatory Diseases; Food Allergens

Cancer; Biomarkers (proteins, peptides)

Proteomics (food proteins and bioactive peptides)

Probiotics, Functional Food.

Indication areas are based on: Regional resources,know how and research co-

operations within the local institutes and companies

Global market expectations

Focus increases the efficiency of Innovation


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J Assouline, Genome-wide measurement of gene expression


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MIAME - cADNmicroarray

MIAME - cADNmicroarray

6STEPS of a Microarray Experiment

Array

Array design

Hybridization protocol

Statistical analysis

Data mining

Image acquisition and

quantification Database building

Filtering

Normalization

HybridizationSample

(S. Sansone)

MIAME Sample QC Sampletreatment

Metadata

mARNs

Extraction protocol

QC Target

preparation

(labeling) - QC

QC

QC

QC


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PROTEOMICS

Sample preparation

1st dimension (1D)

Gel-based Gel free

2nd dimension (2D)

Detection of protein spots Imaging analysis & 2D gel databases

Spot handling: excision, in gel digestion


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MIAPE-GE


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Proteomics


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Need of standards in Proteomics

HUPO-PSI Organization Standard data formats

MIAPEs

PEFF: A Common Sequence DatabaseFormat in Proteomics

PRIDE

Standard data format converters


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2011 dissemination


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Future visionFuture vision

Continue work on important data analysis problems in

bioinformatics and language technology

Applications, including fielded and commercialized ones

Theory and method development

Collaboration across units, disciplines, industry

Future emphasis on

Mining rich public biological databases

Discovery of patterns in complex irregular structures,

discovery of similarities and analogies

Methods forsemantic analysis of large text collections

language and domain-independence, efficiency


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The FUTURE RESEARCH

POLICY Enhance volunteer laboratory work

(undergraduates, graduates master and

PhD students, postdocs)


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USAMV Rector supports

Doru PAMFIL, PhD an accomplished professor of

genetics, he has expertise in the management of manynational and international projects and veteran of large,public research institutions.

Throughout his career he has focused onexpanding undergraduate research opportunitiesand improving the education and professionalexperience of graduate students, with a focus on

underrepresented groups.

Pamfils academic work focuses on Biotechnology(marker assisted selection, detection of GMOs,genetic transformation, micropropagation,microbiology). The GMOs Laboratory CERTOMG -was included in EUROPEANNETWORK OF GMOLABORATORIES (ENGL) in 2009

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"The problem solving, systems thinking, and teamwork aspects of biotechnology can benefit all students,whether or not they ever pursue an scientific career," said Doru PAMFIL, Rector of the University of

Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, head of INSTITUTE OF LIFE SCIENCES(LIS).

"An biotechnology education and science curricula that does not include at least some exposure tobiotechnology is a lost opportunity for students and for the nation."


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Helping Hands for AddressingHelping Hands for AddressingExcellence in Research, EducationExcellence in Research, Education

and Training Needs forand Training Needs for

BiotechnologyBiotechnology

For more information on

future partnerships, contactDr. Ramona SUHAROSCHI

Email: [email protected]

Tel/fax:+40 264 425575

Calea Floresti 64

400509, Cluj-Napoca

For more information on

future partnerships, contactDr. Ramona SUHAROSCHI

Email: [email protected]

Tel/fax:+40 264 425575

Calea Floresti 64

400509, Cluj-Napoca

lab presentation 2011

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