Speakers List, Their Abstracts, Photos and brief Curriculum Vitae

1. Dr. Ganesh M Kishore: CEO of Malaysian Government Investment Branch focused on investing biotechnology projects in India. He is also the Managing Director of Burrell and Burrell Life Sciences Investment Company. Here are his suggested topics.

Title of the talk: Life Sciences - delivering on its commitments; a broad overview describing incredible progress across health and wellness; industrial and environment; agriculture and nutrition sectors and describing emerging areas of importance; impact on businesses. ABSTRACT:

Biotechnology and Life Sciences have had an incredible run during the last three decades. The unraveling of the human genome and the genomes of several organisms, the synthesis of whole genome of bacteria and our understanding of the complexity of biological processes and systems, have laid the foundation for a discipline that will serve humanity for generations to come. Biotechnology industry is already addressing multiple societal needs – feeding the world in a cost effective and sustainable fashion, revitalizing the environment, decentralized production of portable energy and addressing issues of human health and wellness. Bioeconomy is estimated to be approximately a fourth of global GDP by 2025 and already attracting a third of global R&D investments.

Until recently, development and deployment of bioindustrial products have been extensions of chemical business paradigm. In health care, it has been about production and use of biomolecules vs. xenobiotics; in agriculture, it has been about discovering genes and proteins for their ability to bring about effective pest control and in industrial biotech, it has been largely geared towards biocatalyst and bioprocess discovery for making known molecules. Essentially, a $100 Billion revenue industry with market capitalization approaching $500 billion (and~ $1 Trillion including acquired companies) has been built.

With emergence of the genomics era, the life sciences industries are entering a new Biotech 2.0 phase. Biotech 2.0 is both enlarging the scope and sectors of the

Bioeconomy as well as the complexity of problems that are being addressed. Biotech 2.0 is transforming biology as a scientific discipline and making a profound impact on peoples’ lives. Critical to the deployment of biotech are regulatory policies – there is global paucity of fact based decision making in this arena and regions that establish a transparent, logical process will have a significant edge in nurturing a robust bioeconomy. Many of these aspects will be discussed during the presentation.

Description of G. Kishore: Dr. Ganesh Kishore has been active in the science and business of biotechnology for more than three decades. Following his PhD in biochemistry from Indian Institute of Science and postdoctoral research fellow at The University of Texas at Austin, he joined Monsanto. During his early years at Monsanto, he developed manufacturing processes for the synthesis of Aspartame, the active ingredient of NutraSweet and Equal. Subsequently, he served as the leader of the plant biotechnology efforts including the discovery and development of ROUNDUP READY technology. This latter technology is the single largest selling Ag bio product and has created (~$40 billion) nearly 60% of all value created by the Ag bio industry to date. Dr. Kishore was one of the architects of the transformation of Monsanto from a commodity chemical giant to an innovation driven life sciences business. Dr. Kishore retired from Monsanto as its Distinguished Science Fellow, Chief Biotechnologist and President of Nutrition in late 2000.

In 2002, Dr. Kishore joined DuPont as the Chief Technology Officer for its Agriculture and Nutrition Platform and subsequently took over the role of Chief Biotechnology Officer for DuPont. During his tenure at DuPont, he forged several strategic relationships and developed a biotechnology strategy within the company. He left DuPont in 2007 to join Burrill & Company as a Managing Director. Dr. Kishore currently serves as the CEO of Malaysian Life Sciences Capital Fund that makes global investments in life sciences. The Fund has made investments ten direct investments in US and Malaysian companies and eighteen indirect investments through the Burrill Life Sciences Fund. These investments include health care, Ag and industrial biotech companies.

Dr. Kishore has authored sixty technical publications and a named inventor on seventy issued patents. He is the recipient of Queeny Award, a prize awarded by Monsanto Company for outstanding contributions to science and technology that are translated to useful societal products. He is a fellow of the St. Louis Science Academy, a member of the Board of Trustees of St. Louis Science Academy, an

Emeritus Board of Overseers of Tufts University, and R & D Advisory Committee of Washington University at St. Louis and other nonprofit institutions. He serves on the Board of Gevo, Glori Oil, Abunda and a board observer of Light Sciences Oncology. He is an Advisor to the Board, Advanta and an entrepreneur who has founded three biotechnology companies, two in USA and one in India. He and his family reside in St. Louis, USA.

Contact Information: G. M. Kishore, 11966 Sackston Ridge Drive, St. Louis, MO 63141. Phone: 314-994-2156-Hme; 314-994-2156-Home-2; 314-308-0399-Cell. Email: (gkishore@aol.com)

2. Peter J. Hudson: FRS, Director of the Huck Institutes of the Life Sciences, Pennsylvania State University, PA 16802, USA.

Title of the talk: Growing issues in the emergence of zoonotic diseases.

Abstract:

There is growing concern about the rate of emergence of novel infections. The zoonotic infections will be reviewed with the objective of identifying pressing needs for research. Most of these infections have spilled over from wildlife and domesticated animals to infect humans but we know very

little about the process of spillover. The data available show a need for more work on vector borne infections, chronic infections and infections that exhibit latency. Disease dynamics is all about transmission and yet we can rarely record the process let alone identify why a small proportion of infectious hosts are responsible for much of the transmission events. These and related issues will be discussed in detail.

Biographical sketch of Peter J. Hudson:

Education and Qualifications

1. Department of Zoology, University of Leeds. UK. 1974. BSc (Hons) 2. Magdalen College, University of Oxford, UK 1979 DPhil

Edward Gray Institute of Ornithology, Department of Zoology

3. Elected fellow of The Royal Society- 2008 FRS

Appointments

1. Director of Huck institutes of Life Sciences 2006-To Date 2. Director Center for Infectious Disease Dynamics 2004-2006 3. Willaman Chair in Biology, Penn State University. 2002- To Date 4. Personal chair in Animal Ecology: University of Stirling UK 1998-2002 5. Reader in Animal Ecology, University of Stirling UK 1995-1998

Five Influential Publications (selected from >200):

1. Hudson, P.J. Dobson, A.P. & Newborn, D. (1998). Prevention of population cycles by parasite removal. Science 282, 2256-2258

2. Boots, M., Hudson, P.J. & Sasaki, A. 2004. Virulence in spatially structured populations. Science 303: 842-844

3. Cattadori, I.M., Haydon, D. & Hudson, P.J. 2004. Parasites and climate synchronize red grouse populations. Nature 433 737-741

4. Lello, J. B. Boag, A. Fenton, I. R. Stevenson, & P. J. Hudson. 2004. Competition and mutualism among the gut helminths of a mammalian host Nature 428, 840-8

5. Hudson, P.J., Rizzoli, A.P., Grenfell, B.T., Heesterbeek, H. & Dobson, A.P. 2002 Ecology of Wildlife Diseases. Oxford University Press, Oxford.

Five Recent Publications

6. Lloyd Smith, J. O., George, D., Pepin, K.M. Pitzer, V.E., Pulliam, J.R., Dobson, A.P., Hudson, P.J. & Grenfell, B.T. 2009. Epidemic dynamics at the human animal interface. Science December 2009

7. Perkins S.E., Cagnacci, F., Stradiotto, A, Arnoldi, D. & Hudson, P.J. (2009). A comparison of social networks derived from ecological data: implications for inferring infectious disease dynamics. Journal of Animal Ecology 78, 1015-1022

8. Rohr, J.R., Raffel, T.R., Romansic, J.M., McCallum, H. & Hudson, P.J. (2008). Evaluating the links between climate, disease spread and amphibian decline. PNAS 105 17436-17441

9. Vandegrift, K.V. Raffel, T. & Hudson, P.J. (2008). Parasites prevent mouse breeding. Ecology 78: 202-9.

10. Luong, L. T., D. A. Grear, and P. J. Hudson. 2009. Male hosts are responsible for the transmission of a trophically transmitted parasite, Pterygodermatites peromysci, to the intermediate host in the absence of sex-biased infection. International Journal for Parasitology 39:1263-1268.

Full list at: http://www.personal.psu.edu/pjh18/ Synergistic Activities:

Public understanding of Science: Recent articles in Newsweek, New York Times, Boston Globe. Daily Telegraph, London Times, Guardian. Radio: NPR news, BBC World at one and Today program. A number of TV programs on the grouse research for BBC, ITN, Sky TV.

Recent professional committees: Contributions to Science Development: National Academy Committee and author of NSF report on the National Ecological Observatory Network (NEON). 2003. Chair of the NERC funding committee on Terrestrial Science, 1998-2001. Royal Society Tropical committee 2003. NSF Visions committee 2006. Member of the NSF climate change delegation to Japan 2005. Contributions to Peer review: Editorial Board of Journal of Animal Ecology, Journal of Helminthology, Ecological Health, Frontiers in Ecology. Special editor PLOS. Regular reviewer for Nature & Science.

Principal Collaborators: Dr Brian Boag Soil and crop protection (retired Scotland), Dr Mike Boots, Biology, University of Sheffield, UK; Dr Andrew Dobson, EEB, Princeton University; Dr Ernie Gould, CEH Oxford, UK; Dr SM Redpath, CEH Banchory UK, Dr Annapaola Rizzoli CEA, Italy; Dr Arne Skorping, Bergen, (Norway); Dr Tellervo Valtonen (Jyvaskyla, Finland).

Graduate and post doctoral advisors:

Graduate advisor: Dr Chris Perrins (EGI, Oxford). retired

Post doctoral advisor: Dr G R Potts (Game Conservancy Trust, Fordingbridge). Retired

Thesis advisor (completed)

Dr Isabella Cattadori: Penn State, Dr Jonathan Fairburn: EcoAdvisor, Dr Susan Haysom, Scottish Natural Heritage, , Dr Justine Irvine: MLURI Edinburgh, Dr Alan Kirby, Dr Jo Lello: University of Wales, Dr Sarah Perkins: University of Wales, Prof Stephen Redpath, University of Aberdeen, Dr Zoe Russell, RSPB, Dr Roberto Rosa: Centro di Ecolgia Alpina, Italy.Dr Harold Watson, Pest Control, London, Dr Peter White: Imperial College, University of London, Dr Laura Saunders: PPD Cambridge, Dr Amanda Fox: Office of the chief scientist, DEFRA, London. Kurt Vandegrift: Wildlife Trust.

Post doctoral workers: Dr Isabella Cattadori, University of Sheffield, Dr Andrew Fenton, Department of Zoology, University of Cambridge, Dr Lucy Gilbert, University of St. Andrews Dr Sandra Lass, Penn State, Dr Grainne Long, Penn State, Dr Lien Luong, Penn State, Dr Karen Laurenson, Serengeti, Tanzania, Dr Kirsty Park, University of Stirling, Dr Sarah Perkins, Cardiff, Wales, Dr Dan Tompkins, Pest control, New Zealand, Dr Simon Thirgood, McLURI, Scotland. Dr Lien Luong, Penn State. Dr Beth Viglotti. Penn State.

Contact Information: Peter J. Hudson, FRS, Willoman Pofessor of Biology, 201 Life Science Building, University Park, PA 16802. Phone: 814 865 6057 Email: pjh18@psu.edu. www.cidd.psu.edu www.huck.psu.edu.

3.Vivek J. Kapur: Professor and Head, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA 16802, USA.

Title of the Talk: Microbial Pathogenomics – A translational approach to developing the next generation of animal and human vaccines and diagnostics. Abstract:

Louis Pasteur, the father of modern microbiology, epitomizes the scientist who conducted “use-inspired research” – basic research that was motivated by practical applications and utility. This two-centuries old approach is helping set the modern day research agenda at the intersection of basic and applied research. During this presentation, I will describe how microbial genomics and its related translational research activities provide excellent examples of the virtues of use-inspired research, the results of which lead not only to a better understanding of basic life processes, but also to improvements in the technology used to enhance animal and human health. BIOGRAPHICAL SKETCH OF DR. VIVEK KAPUR

EDUCATION/TRAINING:

Univ. Agricultural Sci., Bangalore, India BVSc 1981-86 Veterinary Medicine Univ. Agricultural Sci., Bangalore, India MVsc 1986-89 Poultry Science Pennsylvania State University, Univ. Park, PA, PhD 1988-91 Veterinary Science

Baylor College of Medicine, Houston, TX, Post-doc 1992-95 Microbial Pathogenesis Professional Positions: 1991-1993: Postdoctoral Research Associate, Department of Pathology, Baylor College of Medicine, Houston, Texas 1993-1995: Research Instructor, Department of Pathology, Baylor College of Medicine, Houston, Texas. 1995-1999: Assistant Professor, Department of Veterinary Pathobiology, University of Minnesota, St.Paul, Minnesota. 1995-present: Director, Advanced Genetic Analysis Center, University of Minnesota, St. Paul, Minnesota. 1999-2003- Associate Professor, Departments of Microbiology and Veterinary Pathobiology, University of Minnesota, St. Paul, Minnesota. 2003-2007: Director, BioMedical Genomics Center, University of Minnesota, Minneapolis, Minnesota 2003-2007- Professor, Department of Microbiology, University of Minnesota, St. Paul, Minnesota. 2007-present- Adjunct Professor, Department of Microbiology, University of Minnesota, St. Paul, Minnesota. 2007-present- Professor and Head, Department of Veterinary. & Biomed. Sciences., Penn State, University Park, PA. Awards, Honors and Other Professional Activities: 2009 Chair, NIH IDM-Q (50) Microbiomes Study Section; co-Chair, IDM-M12 Study Section; Chair, Scientific Advisory Board PI2 program, Genome Canada 2008 Pasteur Veterinary Award, International Society for Genomics and Evolutionary Microbiology; Elected, ISOGEM Distinguished Fellow; Chair, NIH IDM-R Study Section; Member, IDM-12 (Phase 2) Study Section; Member (ad-hoc) IDM-P Study Section; Chair, Scientific Advisory Board PI2 program, Genome Canada 2007-Chair, NIH IDM-R Study Section; co-chair, Microbial Genomes 2007, Cambridge UK; Chair, Scientific Advisory Board PI2 program, Genome Canada 2006: Chair, NIH IDM-M Study Section; Chair, NIH IDM-R Study Section 2005: co-Organizer, Genomes 2005, Halifax, Nova Scotia; Chair, NIH IDM-M Study Section; USDA-NRI Study Section Member 2004: co-Organizer, Genomes 2004, Cambridge, UK; Chair, NIH IDM-M Study Section; SSS-Q Study Section Member; co-founder Syntiron, LLC 2003: NIH SBIR/ZRG-1 Study Section Member; NIH RCE in Bio-defense Special Study Section Member; USDA-National Research Initiative Study Section Member; co-Founder, ANDX Inc. 2002-3: co-chair ASM/TIGR Microbial Genomes Conference; NIH Genome Study Section Member (ad-hoc) 2002: Schofield Medal, University of Guelph

2001-2: co-chair of USDA microbial genomics committee 2000: Pfizer Award for Research Excellence; Outstanding Achievement Award, University of Minnesota 1997: College of Veterinary Medicine Award for Graduate Education; Merck Award for Creativity in Teaching; co-Founder, Integrative Genomics, LLC 1990: B. Shivappa gold medal for overall academic achievement, and, University gold medal for overall academic achievement, Univ. Agric. Sci., India B. Selected Peer-reviewed Publications since 2004: 92. Abrahamsen MS, Templeton TJ, Enomoto S, Abrahante JE, Zhu G, Lancto CA, Deng M, Liu C, Widmer G, Tzipori S, Buck GA, Xu P, Bankier AT, Dear PH, Konfortov BA, Spriggs HF, Iyer L, Anantharaman V, Aravind L, Kapur V. 2004. Complete genome sequence of the apicomplexan, Cryptosporidiumparvum. Science 304(5669):441-5. 93. Stratmann J, Strommenger B, Goethe R, Dohmann K, Gerlach GF, Stevenson K, Li LL, Zhang Q, Kapur V, and Bull TJ. 2004. A 38-kilobase pathogenicity island specific for Mycobacterium avium subsp.paratuberculosis encodes cell surface proteins expressed in the host. Infect. Immun. 72:1265-74. 96. Motiwala AS, Amonsin A, Strother M, Manning EJ, Kapur V, Sreevatsan S. 2004. Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis isolates recovered from wild animal species. J.Clin.Microbiol. 42(4):1703-12. 98. Xu, P., G. Widmer, Y. Wang, L. S. Ozaki, J. M. Alves, M. G. Serrano, D. Puiu, P. Manque, D. Akiyoshi, A. J. Mackey, W. R. Pearson, P. H. Dear, A. T. Bankier, D. L. Peterson, M. S. Abrahamsen, V. Kapur, S. Tzipori, and G. A. Buck. 2004. The genome of Cryptosporidium hominis. Nature 431:1107-12. 103. Munir, S., J. M. Sharma, and V. Kapur. 2005. Transcriptional response of avian cells to infection with Newcastle disease virus. Virus Res 107:103-8. 107. Gadgil, M., W. Lian, C. Gadgil, V. Kapur, and W. S. Hu. 2005. An analysis of the use of genomic DNA as a universal reference in two channel DNA microarrays. BMC Genomics 6:66. 108. S. C. Olsen. 2005. Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis. J Bacteriol 187:2715-26. 109. Hirt, H., D. A. Manias, E. M. Bryan, J. R. Klein, J. K. Marklund, J. H. Staddon, M. L. Paustian, V. Kapur, and G. M. Dunny. 2005. Characterization of the pheromone response of the Enterococcus faecalis conjugative plasmid pCF10: complete sequence and comparative analysis of the transcriptional and

phenotypic responses of pCF10-containing cells to pheromone induction. J Bacteriol 187:1044-54. 112. Li, L., J. P. Bannantine, Q. Zhang, A. Amonsin, B. J. May, D. Alt, N. Banerji, S. Kanjilal, and V. Kapur. 2005. The complete genome sequence of Mycobacterium avium subspecies paratuberculosis. Proc Natl Acad Sci USA. 102:12344-12349. 115. Munir, S., K. Kaur, and V. Kapur. 2006. Avian metapneumovirus phosphoprotein targeted RNA interference silences the expression of viral proteins and inhibits virus replication. Antiviral Res. 69:46-51. 120. Motiwala, A. S., H. K. Janagama, M. L. Paustian, X. Zhu, J. P. Bannantine, V. Kapur, and S. Sreevatsan.2006. Comparative Transcriptional Analysis of Human Macrophages Exposed to Animal and Human Isolates of Mycobacterium avium Subspecies paratuberculosis with Diverse Genotypes. Infect Immun 74:6046-56. 123. Treff, N. R., R. K. Vincent, M. L. Budde, V. L. Browning, J. F. Magliocca, V. Kapur, and J. S. Odorico.2006. Differentiation of embryonic stem cells conditionally expressing neurogenin 3. Stem Cells 24:2529-37. 130. Bannantine, J. P., M. L. Paustian, W. R. Waters, J. R. Stabel, M. V. Palmer, L. Li, and V. Kapur. 2008. Profiling bovine antibody responses to Mycobacterium avium subsp. paratuberculosis infection by using protein arrays. Infect Immun 76:739-49. 136. Zhu X, Tu ZJ, Coussens PM, Kapur V, Janagama H, Naser S, Sreevatsan S. 2008. Transcriptional analysis of diverse strains Mycobacterium avium subspecies paratuberculosis in primary bovine monocyte derived macrophages. Microbes Infect. 10:1274-1282. Issued Patents: 5 issued US patents: 5,641,491; 6,030,835; 6,096,322; 6,495,334; 7,074,559; 7,141,033; and related International patents Current Research Support (partial listing of projects with 10% or greater effort only) 200735205-11626 Kapur (PI) 12/15/2009 – 12/31/2012 USDA-CSREES Molecular Responses to Respiratory Infections Role: PI Total Award: $375,0000 2008-35605-14243 Kapur (PI) 04/15/08– 04/14/2011 USDA-NRI-CSREES JDIP: Johne’s Disease Integrated Program in Research, Education and Extension

Role: PI Total Award: $4,800,000 2008-35605-14243 Kapur (PI) 04/15/08– 04/14/2011 USDA-APHIS Johne’s Disease Vaccine Development Program Role: PI Total Award: $550,000 Kapur (Enck) 07/01/2008-06/30/2009 PA Department of Agriculture Diagnose Disease of Animals and Coordinate Diagnostic Effort Role: Co-PI Total Award $3,200,000 N00014-04-1-0659 Kapur (Kennedy) 05/01/07 – 04/30/11 DHS/NAVY Post-Harvest Food Protection and Defense Center Role: Co-PI, Lead, Detection & Diagnostics Total Award $15,000,000

4. James E. Womack: Distinguished Professor, W.P. Luse Professor, Department of Veterinary Pathobiology and Faculty of Genetics. Director, Center for Animal Biotechnology and Genomics, Texas A&M University, College Station, Texas 77843, USA.

Title of the Talk: Global Challenges in Livestock Genomics: Mining Genomes for Biologically Important Variation.

Abstract:

Sequencing of whole genomes has been extended to several of our livestock species and high-resolution maps exist for others. Tools are available for functional genomics, as well as whole genome screening for single nucleotide polymorphisms (SNPs) and copy number variants (CNVs). Despite the availability of these and other genomics tools and the mapping of hundreds of

quantitative trait loci (QTL) in livestock species, very few variant genes underlying these trait differences have been identified. In this presentation, the development of the bovine sequencing project will be used as an example to illustrate the magnitude of the effort and the salient lessons learned from whole genome sequencing. The importance of comparative genomics and the functional biological significance of chromosomal breakage in mammalian evolution will be discussed.

The second part of this presentation will focus on our efforts to identify and characterize genomic variation in bovine genes involved in host-resistance to pathogens. Data will be presented on the Toll-like receptor (TLR) gene family and related genes. These data include SNP discovery, projections of protein alterations, and haplotype structure in Bos taurus, Bos indicus, and composite breeds. Data will also be presented on the family of cathelicidin (CATHL) genes that code antimicrobial peptides. Variation in these genes includes SNPs, insertion-deletions (INDELS) as well as CNVs. The potential impact of identification of genes for host-resistance will be discussed, both in terms of breeding for disease resistant animals and for discovery of new approaches to development of vaccines and therapeutics.

Description of James Womack:

Dr. James E. (Jim) Womack holds the title of Distinguished Professor at Texas A&M University and is the W.P. Luse Professor of Pathobiology in the College of Veterinary Medicine. He is a member of the Faculty of Genetics and holds a joint academic appointment in the Department of Medical Biochemistry and Genetics. He is Director of the Center for Animal Biotechnology and Genomics. Dr. Womack holds degrees from Abilene Christian University (B.S.1964) and Oregon State University (Ph. D.1968). He serves as Coordinator for the USDA-NRSP8 Cattle Genome Program, and has served as President of the International Society for Animal Genetics, the American Genetics Association and the Texas Genetics Society. He is a member of the National Academy of Sciences USA, a Fellow in the AAAS, and recipient of the 1994 CIBA Prize for research in animal health and the 2001 Wolf Prize in agriculture. He has published, with students and associates, more than 300 peer reviewed articles in scientific journals. His research interests are comparative genomics, mapping the bovine genome, and the genetic basis of disease resistance in mammals.

Biographical Sketch of James Womack:

NAME

Womack, James E.

POSITION TITLE

Distinguished Professor

EDUCATION/TRAINING

INSTITUTION AND LOCATION DEGREE YEAR

FIELD Y

Abilene Christian College B.S. 1964 Mathematics Ed.

Oregon State University Ph.D. 1968 Genetics

A. Positions and Honors 1968-1971 Assistant Professor, Abilene Christian College

1971-1973 Associate Professor, Abilene Christian College

1973-1975 Visiting Scientist, The Jackson Laboratory

1975-1977 Staff Scientist, The Jackson Laboratory

1977-1983 Associate Professor, Texas A&M University

1983- Present Professor, Veterinary Pathology, Texas A&M University

1987-Present W.P. Luse Professor, Texas A&M University

1989-1996 Director, Center for Animal Genetics, Institute of Biosciences & Technology

2001-Present Distinguished Professor, Texas A&M University Director, Center for Animal Biotechnology and Genomics, Texas A&M University Other Experience and Professional Memberships

1978-Present Texas Genetics Society (President, 1989)

1981-Present American Genetic Association (President, 1985; Executive V.P. 1996-2003)

1993-Present Coordinator, USDA-NRSP8 Bovine Genome Program

1988-Present International Society for Animal Genetics (President, 2000-Present)

1993-Present Human Genome Organization (HUGO)

2001 Nominating Committee, National Academy of Sciences USA

Editorial Boards (past and present)

Genomics, Journal of Heredity, Biochemical Genetics, Animal Genetics, Mammalian Genome, Genome Research, Animal Biotechnology

Honors

Alumni Citation Award, Abilene Christian University, 1983

Faculty Distinguished Achievement Award for Research, Texas A&M University, 1987

Carrington Award for Research in Cell Biology, 1990

McMaster Fellow, CSIRO, Australia, 1990

CIBA Prize for Research in Animal Health, 1993

Outstanding Texas Geneticist, Texas Genetics Society, 1996

Fellow, American Association for the Advancement of Science, 1999 National Academy of Sciences, USA, 1999

Wolf Prize in Agriculture, 2001

Editorial Boards (past and present)

Genomics, Journal of Heredity, Biochemical Genetics, Animal Genetics, Mammalian Genome, Genome Research,

Animal Biotechnology

Honors

Alumni Citation Award, Abilene Christian University, 1983

Faculty Distinguished Achievement Award for Research, Texas A&M University, 1987

Carrington Award for Research in Cell Biology, 1990

McMaster Fellow, CSIRO, Australia, 1990

CIBA Prize for Research in Animal Health, 1993

Outstanding Texas Geneticist, Texas Genetics Society, 1996

Fellow, American Association for the Advancement of Science, 1999 National Academy of Sciences, USA, 1999

Wolf Prize in Agriculture, 2001

Distinguished Service Award, Texas Genetics Society, 2006

Outstanding Alumnus of the Year, Abilene Christian University, 2006 Dean’s Impact Award, CVM, Texas A&M University, 2007 Bush Excellence Award for Faculty in International Research, Texas A&M University, 2008 Student-Led Award for Teaching Excellence, Texas A&M University System, 2009 Advisory Board for the College of Arts and Sciences, Abilene Christian University, 2009

B. Selected peer-reviewed publications (from total of 320+)

Rexroad, C.E. III, and Womack, J.E.: Parallel RH mapping of BTA1 with HSA3 and HSA21. Mammalian Genome 10:1095-1097, 1999.

O’Brien, S.J., Menotti-Raymond, M., Murphy, W.J., Nash, W.G., Wienberg, J., Stanyon, R., Copeland, Neal G., Jenkins, N.A., Womack, J.E., and Graves, J.A.M.: The promise of comparative genomics in mammals. Science 286:458-481, 1999.

Ozawa, A., Band, M.R., Larson, J.H., Donovan, J., Green, C.A., Womack, J.E., and Lewin, H.A.: Comparative organization of cattle chromosome 5 revealed by comparative mapping by annotation and sequence similarity and radiation hybrid mapping. Proc. Natl. Acac. Sci. USA 97:4150-4155, 2000.

Band, M.R., Larson, J.H., Rebeiz, M., Green, C.A., Heyen, D.W., Donovan, J., Windish, R., Steining, C., Mahyuddin, P., Womack, J.E., and Lewin, H.A.: An ordered comparative map of the cattle and human genomes. Genome Research 1359-1368, 2000.

Goldammer, T., Kata, S.R., Brunner, R.M., Dorroch, U., Sanftleben, H., Schwerin, M., and Womack, J.E.: A comparative radiation hybrid map of bovine chromosome 18 and homologous chromosomes in human and mice. Proc. Natl. Acad. Sci. USA, 99:2106-2111, 2002.

Takeda, H., Takami, M., Oguni, T., Tsuji, T., Yoneda, K., Sato, H., Ihara, N., Itoh, T., Kata, S.R., Mishina, Y., Womack, J.E., Moritomo, Y., Sugimoto, Y. and Kunieda, T.:

Positional cloning of the gene LIMBIN responsible for bovine chondrodysplastic dwarfism. Proc. Natl. Acad. Sci. USA, 99:10549-10554, 2002.

Winter, A., Krämer, W., Werner, F.A., Kollers, S., Kata, S., Durstewitz, G., Buitkamp, J., Womack, J.E., Thaller, G., and Fries, R.: Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proc. Natl. Acad. Sci. USA 97:9300-9305, 2002.

White, S.N., Kata, S.R., and Womack, J.E.: Comparative fine maps of bovine toll-like receptor 4 and toll-like receptor 2 regions. Mammalian Genome 14:149-155, 2003.

White, S.N., Taylor, K.H., Abbey, C.A., and Gill, C.A., and Womack, J.E.: Haploytype variation in bovine Toll-like receptor 4 and computational predication of a positively selected ligand-binding domain. Proc. Natl. Acad. Sci. USA 100:10364-10369, 2003.

Larkin, D.M., der Wind, A.E., Rebeiz, M., Schweitzer, P.A., Bachman, S., Green, C., Wright, C.L., Campos, E.J., Benson, L.D., Edwards, J., Liu, L., Osoegawa, K., Womack, J.E., de Jong, P., and Harris, A.L.: A cattle-human comparative map built with cattle BAC-ends and human genome sequence. Genome Research 13:1966-1973, 2003.

Raudsepp T., Lee, E.-J., Kata, S.R. Brinkmeyer, C., Mickelson, J.R., Skow, L.C., Womack, J.E. and Chowdhary, B.P.: Exceptional conservation of horse-human gene order on X chromosome revealed by high-resolution radiation hybrid mapping. Proc. Natl. Acad. Sci. USA 2386-2391, 2004.

Raudsepp, T., Santani, A., Wallner, B., Kata, S.R., Ren, C., Zhang, H.-B., Womack, J.E., Skow, L.C., and Chowdhary, B.P.: A detailed physical map of the horse Y chromosome. Proc. Natl. Acad. Sci. USA 101:9321-9326, 2004.

Everts-van der Wind, A., Kata, S.R., Band, M.R., Rebeiz, M., Larkin, D.M., Everts, R., Green, C.A., Liu, L., Natarajan, S., Goldammer, T., Lee, J.H., McKay, S., Womack, J.E. and Lewin, H.A.: A 1463 gene cattle-human comparative map with anchor points defined by human genome sequence coordinates. Genome Research 14:1424-1437, 2004.

Murphy, W.J,. Larkin D.M., Everts-van der Wind A., Borque G., Tesler G., Auvil L., Beever J.E., Chowdhary B.P., Galibert F., Gatzke L., Hitte C., Meyers S.N., Milan D., Ostrander E.A., Pape G., Parker G.H., Raudseep T., Rogatcheva M.B., Schook L.B., Skow L.C., Welge M., Womack J.E., O’Brien S.J., Pevzner P.A., and Lewin H.A.: Dynamics of mammalian chromosome evolution inferred from multispecies comparative maps. Science 309, 613-617, 2005.

Everts-van der Wind , A., Larkin, D.M., Green, C.A., Elliott, J.S., Olmstead, C., Chiu, R., Schein, J.E., Marra, MA, Womack, J.E., Lewin, H.A.: A high-resolution whole-genome cattle-human comparative map reveals details of mammalian chromosome evolution. Proc Natl Acad Sci USA;102:18526-18531, 2005.

Womack, J.E.: Advances in livestock genomics: Opening the barn door. Genome Research 15:1699-1705, 2005.

Sugimoto, M., Fujikawa A., Womack, J.E., Sugimoto Y.: Evidence that bovine forebrain embryonic zinc finger-like gene influences immune response associated with mastitis resistance. Proc Natl Acad Sci USA 103:6454-6459, 2006.

Womack, J.E.: The impact of sequencing the bovine genome. Australian Journal of Experimental Agriculture 46:151-153, 2006.

Callicott, R. and Womack, J.E.: Real-time PCR assay for measurement of mouse telomeres. Comp Med, 56:17-22, 2006.

Cargill, E.J., Paetzold, L., and Womack, J.E.: Radiation hybrid mapping and comparative sequence analysis of bovine RIG-I and MAVS genes. DNA Sequence 17:314-318, 2006.

Cargill, E.J. and Womack, J.E.: Detection of polymorphisms in bovine toll-like receptors 3, 7, 8, and 9. Genomics 89:745-755, 2007.

Ghebranious N, Burmester JK, Glurich I, McPherson E, Ivacic L, Kislow J, Rasmussen K, Kumar V, Raggio CL, Blank RD, Jacobsen FS, Faciszewski T, Womack J, Giampietro PF: Evaluation of SLC35A3 as a candidate gene for human vertebral malformations. Am J Med Genet Part A 140A:1346-1348, 2006.

Taylor, K.H., Taylor, J.F., White, S.N. and Womack, J.E.: Identification of genetic variation and putative regulatory regions in bovine CARD15. Mammalian Genome 17:892-901, 2006.

Seabury, C.M., Cargill, E.J., and Womack, J.E.: Sequence variability and protein domain architectures for bovine Toll-like receptors 1, 5 and 10. Genomics 90:502-515, 2007.

Seabury, C.M., Gill,C.A., Templeton, J.W., Dyar, J.B., Derr, J.N., Adelson, D.L., Owens, K.E., Davis, D.S., Kraemer, D.C., Womack, J.E.: Molecular Characterization of the Rocky Mountain (Cervus elaphus nelsoni) PRNP Putative Promoter. Journal of Heredity, 98:678-686, 2007.

Bovine Genome Sequencing and Analysis Consortium: 315 authors including Womack, J.E.: The genome sequence of taurine cattle: a window to ruminant biology and evolution. Science 324:522-528, 2009.

Bovine HapMap Consortium. 90 authors including Womack, J.E.: Genome-wide survey of SNP variation uncovers the genetic structure of cattle breeds. Science 324:528-532, 2009.

Goldammer, T., Brunner, R.M., Rebl, A., Wu, C.H., Nomura, K., Hadfield, T., Gill, C., Dalrymple, B.P., Womack, J.E. and Cockett, N.E.: A high-resolution radiation hybrid map of sheep chromosome X and comparison with human and cattle. Cytogenetics and Genome Res 125:40-45, 2009.

Gillenwaters, E.N., Seabury, C.M., Elliott, J.S. and Womack, J.E.: Sequence analysis and polymorphism discovery in 4 members of the bovine cathelicidin gene family. J.Hered. 100:241-245, 2009.

5. Harris A. Lewin: Gutgsell Endowed Chair, Director, Institute for Genomic Biology. Professor of Immunogenetics. University of Illinois. Urbana, IL-61801, USA.

Title of the Talk:

Abstract:

Description of Harris Lewin:

Harris Lewin is Center for Advanced Study and Gutgsell Endowed Professor of Immunogenetics, with a primary appointment in the Department of Animal Sciences at the University of Illinois at Urbana-Champaign. Lewin served as Director of the University of Illinois Biotechnology Center, Founding Director of the W. M. Keck Center for Comparative

and Functional Genomics, and is currently Founding Director of the Institute for Genomic Biology.

Professor Lewin’s current research interest is mammalian comparative and functional genomics. He and his team are studying how mammalian genomes evolve and the role of chromosome rearrangements in adaptation and speciation. Lewin’s research led to the identification of a gene responsible for resistance and susceptibility to bovine leukemia virus infection, the development of high-density comparative maps for mammalian genomes, and co-discovery of a gene affecting milk fat composition. In addition, his group produced the first large-scale cattle cDNA and oligo microarrays, which he and his collaborators have used to study the genomic effects of nuclear transfer cloning, the development and involution of the mammary gland, and the dietary effects on gene expression in the periparturient period. Lewin is Associate Editor of the journal Animal Biotechnology and serves on the Editorial Board of Physiological Genomics and Annual Reviews of Genomics and Human Genetics. In 2004, he was elected as a Fellow of the American Association for the Advancement of Science and in 2007 he was elected as a foreign member of the Royal Swedish Academy of Agriculture and Forestry.

BIOGRAPHICAL SKETCH

NAME Lewin, Harris A.

POSITION TITLE Professor of Animal Sciences Director, Institute for Genomic Biology

eRA COMMONS USER NAME EDUCATION/TRAINING

INSTITUTION AND LOCATION DEGREE YEAR FIELD OF STUDY

Cornell University, Ithaca, NY Cornell University, Ithaca, NY

University of California, Davis, CA

B.S.

M.S.

Ph.D.

1979

1981

1984

Animal Science Animal Breeding &

Genetics

Immunology

PROFESSIONAL EXPERIENCE:

Professor, Center for Advanced Study, University of Illinois, 2009-present

Director, The Institute for Genomic Biology, 2003-present

Research Professor, Microelectronics Laboratory, 2002-present

Gutgsell Endowed Chair, 1999-present

Professor of Animal Sciences, Department of Animal Sciences, University of Illinois, 1994-present

Director, The W. M. Keck Center for Comparative and Functional Genomics, 1998-2003

Director, The University of Illinois Biotechnology Center, 1996-2000

Visiting Associate Professor, Section of Molecular Biology, UCLA, 1991

Visiting Scientist, Dept. Animal Genetics, National Veterinary Institute, Oslo, Norway, Aug. 1989

RECOGNITIONS AND OUTSTANDING ACHIEVEMENTS:

Alltech Distinguished Lecturer in Nutrigenomics, University of Kentucky, Lexington, Kentucky, 2009; Co-organizer, Bovine Genome Consortium Meeting, Cold Spring Harbor, NY, 2009; Foreign Member, Royal Swedish Academy of Agriculture and Forestry, elected 2007; Co-chair, International Conference on Pathways, Networks and Systems Medicine, Greece, 2007-present; Fellow, American Association for the Advancement of Science, 2004, ACES Team Award for Excellence in Research, 2003; C. R. Henderson Lecture in Animal Breeding and Genetics, Cornell University, 2000; Session Chair (Comparative Genomics and Evolution), Genome Sequencing and Biology Meeting, Cold Spring Harbor, 2001; Burroughs Wellcome Visiting Professorship in Basic Biomedical Sciences (Washington State University), 1999-2000; Arnold O. Beckman Research

Award, University of Illinois at Urbana-Champaign, 1997; Paul A. Funk Recognition Award, College of Agriculture, Consumer and Environmental Sciences, University of Illinois at Urbana-Champaign 1996; H.H. Mitchell Award for Excellence in Graduate Teaching and Research, Department of Animal Sciences, University of Illinois,1995; University Scholar, University of Illinois at Urbana-Champaign, 1993; Young Faculty Award for Excellence in Research, College of Agriculture, University of Illinois, 1992.

MEMBERSHIPS (SELECTED):

American Association for the Advancement of Science; American Association of Immunologists; American Association of Veterinary Immunologists; International Society for Animal Genetics; American Association of Animal Science; Editorial Board: Physiological Genomics, 1999-present; Associate Editor: Animal Biotechnology, 1990-present; Editorial Committee: Annual Review of Genomics and Human Genetics, 2009-present.

OTHER ACTIVITIES (SELECTED):

Member, Domestic Animal Genome Sequencing Committee, International Society for Animal Genetics, 2002-current; Member, Advisory Board, Biosciences Division, Argonne National Laboratory, 2005-2007; Scientific Advisory Board, Pyxis Genomics, Chicago, IL, 2000-2007; Scientific Advisory Board, Burrill and Company, Animal Health Venture Fund, 1999-2002; Chairman, Scientific Advisory Board, GenoMar AS (Norway), 1997-2005; ISAG Standing Committee on Cattle, Sheep and Goat Gene Mapping (elected), 1996-present, Veterinary Immunology Subcommittee, American Association of Immunologists (appointed), 1993-1996; (reappointed), 1997-2000; Chairman, USDA Committee on Animal Genome Database System, 1997-1999; Governor’s Biotechnology Advisory Council, 1997-1999 (appointed); Scientific Program Committee for the 26th Meeting of the International Society for Animal Genetics, 1998, New Zealand (appointed); External Advisory Committee, Food Animal Biotechnology Center, University of Minnesota, 1994-2000; Editor: National Animal Genome Research Program (NAGRP) Newsletter (1993-1996); Peer Review Panel, USDA/CSRS National Research Initiative Competitive Grants Program (Animal Molecular Genetics and Gene Mapping), 1990, 1993; NAGRP Genome Executive Committee, 1993-1994.

SELECTED PUBLICATIONS: (selected since 1993, from a total of 152 papers and 21 book chapters)

Da, Y., R.D. Shanks, J.A. Stewart and H.A. Lewin. 1993. Milk and fat yields decline in bovine leukemia virus-infected dairy cattle with persistent lymphocytosis. Proc. Natl. Acad. Sci. (USA) 94:6538-6541.

Xu, A., C. Park and H. A. Lewin. 1994. Both DQB genes are expressed in a BoLA haplotype carrying a duplicated DQ region. Immunogenetics 39:316-321.

Jarrell, V. L., H. A. Lewin, Y. Da and M. B. Wheeler. 1995. Gene-centromere mapping of bovine DYA, DRB3, and PRL using secondary oocytes and first polar bodies: evidence for four-strand double crossovers between DYA and DRB3. Genomics 27: 33-39.

Mirsky, M. L., C. A. Olmstead, Y. Da and H. A. Lewin. 1996. The prevalence of proviral bovine leukemia virus in peripheral blood mononuclear cells of naturally infected cows at different stages of infection. J. Virol. 70:2178-2183.

Band, M., J. H. Larson, J. E. Womack, and H. A. Lewin. 1998. A radiation hybrid map of BTA23: identification of a chromosomal rearrangement leading to separation of the cattle MHC class II subregions. Genomics 53: 269-275.

Park, C. and H. A. Lewin. 1999. Fine mapping of a region of variation in recombination rate on BTA23 to the D23S22-D23S23 interval using sperm typing and meiotic breakpoint analysis. Genomics 59:143-149.

Ozawa, A., M. R. Band, J. H. Larson, J. Donovan, C. A. Green, J. E. Womack and H. A. Lewin. 2000. Comparative organization of cattle chromosome 5 revealed by COMPASS and radiation hybrid mapping. Proc. Natl. Acad. Sci. (USA) 11:4150-4155.

Band, M. R., C. Olmstead, R. E. Everts, Z. L. Liu and H. A. Lewin. 2002. A 3800 gene microarray for cattle functional genomics: comparison of gene expression in spleen, placenta and brain. Anim. Biotechnol. 13:163-172.

Mwangi, W., W. C. Brown, H. A. Lewin, C. J. Howard, J. C. Hope, T. V. Baszler, P. Caplazi, J. Abbot and G. H. Palmer. 2002. DNA encoded FLT3 ligand and GM-CSF increase dendritic cell recruitment to the inoculation site and enhance antigen-specific CD4+ T cell responses induced by DNA vaccination of outbred animals. J. Immunol. 169:3837-3846.

Larkin, D. M., A. Everts-van der Wind, M. Rebeiz, P. A. Schweitzer, S. Bachman, C. Green, C. L. , Wright, E. J. Campos, L. D. Benson, J. Edwards, L. Liu, K. Osoegawa, J. E. Womack, P. de Jong, and H. A. Lewin. 2003. A cattle-human comparative map built with cattle BAC-ends and human genome sequence. Genome Res. 13:1966-1972.

Lewin, H. A. 2004. The future of cattle genomics: the beef is here. Cytogenetics and Genome Res. 102:10-15.

Everts-van der Wind, A., S. R. Kata, M. R., Band, M.Rebeiz, D. M., Larkin, R. E. Everts, C. A. Green, L.Liu, S. Natarajan, T. Goldhammer, J. H. Lee, S. Mckay, J. E. Womack and H. A. Lewin. 2004. A 1,463 gene cattle-human comparative map with anchor points defined by human genome sequence coordinates. Genome Research 14:1424-1437.

Kumar, C. G., R. LeDuc, G. Gong, L. Roinishivili, H. A. Lewin and L. Liu. ESTIMA, a tool for EST management in a multi-project environment. 2004. BMC Bioinformatics 5:176.

Murphy, W. J., D. M. Larkin, A. Everts-van der Wind, G. Bourque, G. Tesler, L. Auvil, J. E. Beever, B. P. Chowdhary, F. Galibert, L. Gatzke, C. Hitte, S. N. Meyers, D. Milan, E. A. Ostrander, G. Pape, H. G. Parker, T. Raudsepp, M. B. Rogatcheva, L. B. Schook, L. C. Skow, M. Welge, J. E. Womack, S. J. O’Brien, P. A. Pevzner and H. A. Lewin. 2005. Dynamics of mammalian chromosome evolution inferred from multispecies comparative maps. Science 309:613-617.

Smith, S. L., R. E. Everts, X. C. Tian, F. Du, L.-Y. Sung, S. L. Rodriguez-Zas, B.-S. Jeong, J.-P. Renard, H. A. Lewin and X. Yang. 2005. Global gene expression profiles reveal significant nuclear reprogramming by the blastocyst stage after cloning. Proc. Natl. Acad. Sci. (USA)102:17582-17587.

Larson, J. H., B. M. Marron, J. E. Beever, B. A. Roe and H. A. Lewin. 2006. Genomic organization and evolution of the ULBP genes in cattle. BMC Genomics, 7:227-241.

Yang, X., S. L. Smith, X. C. Tian, H. A. Lewin, and T. Wakayama. 2007. Nuclear reprogramming in cloned embryos and its implications for therapeutic cloning. Nature Genetics 39:295-302..

Snelling, W. M., R. Chiu, J. E. Schein, M. Hobbs, C. A. Abbey, D. L. Adelson, J. Aerts, G. L. Bennett, I. E. Bosdet, M. Boussaha, R. Brauning, A. R. Caetano, M. M. Costa, A. M. Crawford, B. P. Dalrymple, A. Eggen, A. Everts-van der Wind, S. Floriot, M. Gautier, C. A. Gill, R. D. Green, R.Holt, O. Jann, S. J. M. Jones, P. J. de Jong, S. M. Kappes, J. W. Keele, D. M. Larkin, H. A. Lewin, J. C. McEwan, S. McKay, M. A. Marra, C. A. Mathewson, L. K. Matukumalli, S. S. Moore, B. Murdoch, F. Nicholas, K. Osoegawa, A. Roy, H. Salh, L. Schibler, R. Schnabel, L. Silveri, L. C. Skow, T. S. Sonstegard, J. Taylor, R. Tellam, C. P. Van Tassell, J. L. Williams, J. E. Womack, N. H. Wye, G.Yang and S. Zhao 2007. A physical map of the bovine genome. Genome Biology 8:R165.

Everts, R. E., P. Chavatte-Palmer, A. Razzak, I. Hue, C. A. Green, R. Oliveira, X. Vignon, S. L. Rodriguez-Zas, X. C. Tian, X. Yang, J.-P. Renard and H. A. Lewin. 2008. Aberrant gene expression patterns in placentomes are associated with phenotypically normal and abnormal cattle cloned by somatic cell nuclear transfer. Physiological Genomics. 33:65-77.

Mansouri-Attia, N., O. Sandra, J. Aubert, S. Degrelle, R. E. Everts, C. Giraud-Delville, Y. Heyman, L. Galio, I. Hue, X. Yang, X. C. Tian, H. A. Lewin and J.-P. Renard. 2009. Endometrium as an early sensor of in vitro manipulation technologies. Proc. Natl. Acad. Sci. (USA) 106:5687-5692.

Tellam, R. L., D. G. Lemay, C. P. Van Tassell, H. A. Lewin, K. C. Worley and C. G. Elsik. 2009. Unlocking the bovine genome. BMC Genomics 10:193.

The Bovine Genome Sequencing and Analysis Consortium, C. G. Elsik, R. L. Tellam and K. C. Worley. 2009. The genome sequence of taurine cattle: A window to ruminant biology and evolution. Science 324:522-527.

Mansouri-Attia, N., J. Aubert, P. Reinaud, C. Giraud-Delville, G. Taghouti, L. Galio, R. E. Everts, S. Degrelle, C. Richard, I. Hue, X. Yang, X. C. Tian, H. A. Lewin, J.-P. Renard and O. Sandra. 2009. Gene expression profiles of bovine caruncular and intercaruncular endometrium at implantation. Physiol. Genomics 39:14-27.

Lewin, H. A., D. M. Larkin, J. Pontius and S. J. O’Brien. 2009. Every genome sequence needs a good map. Genome Res.19:1925-1928.

Donthu, R., D. M. Larkin, M. P. Heaton and H. A. Lewin. 2009. Discovery, validation and characterization of 1039 cattle single nucleotide polymorphisms. Anim. Genet. (in press).

6. Rosane Oliveira, D.V.M., PhD, Department of Animal Sciences, University of llinois at Urbana-Champaign. Urbana-61801, USA.

We are waiting for her information Contact Information: 210 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801 Phone: (217) 244-6745; Fax: (217) 244-5617 E-mail: (rosane@illinois.edu).

7. Mitchell Abrahamsen: Vice President of Research for Hills Pet Nutrition. Email: (abe@umn.edu).

8. Bhushan Jayarao: Professor of Veterinary and Biomedical Sciences. Pennsylvania State University, University Park, PA 16802, USA. Title of the Talk: Antimicrobial Resistant Bacteria and Food Safety: An Emerging Global Issue Abstract:

Over the last two decades the use of antimicrobials in animal agriculture and the emergence of antibiotic resistant bacteria have received worldwide attention. In many countries, antimicrobials are used in food animal production for therapeutic, prophylactic, or growth promotion purposes. There are reports that state nearly 90% of the antimicrobials used in food animal production are for growth promotion and prophylaxis. This extensive use is perhaps one of the

likely contributors to the emergence, selection, and dissemination of antimicrobial-resistant bacteria and resistance determinants. Public health experts are of the opinion that use of antimicrobials in food animals creates an important source of antimicrobial-resistant bacteria that can spread to humans through the food supply. The World Health Organization states that improved management of the use of antimicrobials in food animals is an important step toward preserving the benefits of antimicrobials for people. In several countries, educational programs targeted at professionals (veterinarians and physicians), food animal producers and public on prudent use of antimicrobials has met with limited success. Further the discovery of new classes of antibiotics to treat and control infectious diseases had not kept pace with the increase in antibiotic resistance. Several organizations and researchers have focused their efforts on developing alternatives to antibiotics. These include probiotics, prebiotics, bacteriophages, natural products, bacteriocins and antimicrobial peptides. The focus of this presentation is to highlight some of the promising alternatives that could replace use of conventional antimicrobials for prophylaxis and growth promotion purposes in animal agriculture in the near future. Biographical Sketch of Dr. Bhushan Jayarao:

EDUCATIONAL BACKGROUND

BVSc & AH 1980 Bachelor of Veterinary Science and Animal Husbandry, Bombay Veterinary College, Mumbai, India.

MVSc 1982 Master of Veterinary Science in Food Hygiene and Veterinary Public Health, Bombay Veterinary College, Mumbai, India.

Ph.D. 1989 Food Microbiology and Epidemiology, University of Veterinary Sciences, Budapest, Hungary.

M.P.H. 1994 Occupational/ Environmental Health and Safety, The University of Tennessee, Knoxville, Tennessee.

CAREER HISTORY Sept. 2008- Aug. 2009 FASS/AAAS Congressional Science Fellow, House of

Representatives, Committee on Energy and Commerce, Subcommittee on Oversight and Investigations, 316 Ford Bldg. Washington DC.

2008- present Professor/ Extension Veterinarian / Interim Head, Bacteriology Section

2002-2007 Associate Professor / Extension Veterinarian, Dept. of Veterinary and Biomedical Sciences, Penn State.

1998-2002 Assistant Professor / Extension Veterinarian, Dept. of Veterinary and Biomedical Sciences, Penn State.

1995–1998 Assistant Professor, Dairy Science Department, South Dakota State University, Brookings, South Dakota.

1991–1995 Research Assistant Professor, Department of Animal Science, The University of Tennessee, Knoxville.

Research and/or Scholarly Publications Houser BA, Donaldson SC, Padte R, Sawant AA, DebRoy C, Jayarao BM. Assessment of phenotypic and genotypic diversity of Escherichia coli shed by healthy lactating dairy cattle. Foodborne Pathog Dis. 2008 Spring;5(1):41-51.

Brooks JW, Jayarao BM. Management practices used by white-tailed deer farms in Pennsylvania and herd health problems. J Am Vet Med Assoc. 2008 Jan 1;232(1):98-104.

Gurjar AA, Hegde NV, Love BC, Jayarao BM. Real-time multiplex PCR assay for rapid detection and toxintyping of Clostridium perfringens toxin producing strains in feces of dairy cattle. Mol Cell Probes. 2008 Apr;22(2):90-5. Epub 2007 Aug 19.

Kehoe SI, Jayarao BM, Heinrichs AJ. A survey of bovine colostrum composition and colostrum management practices on Pennsylvania dairy farms. J Dairy Sci. 2007 Sep;90(9):4108-16.

Gurjar AA, Yennawar NH, Yennawar HP, Rajashankar KR, Hegde NV, Jayarao BM. Expression, crystallization and preliminary X-ray diffraction studies of recombinant Clostridium perfringens beta 2-toxin. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt 6):484-7. Epub 2007 May 5.

Sawant AA, Hegde NV, Straley BA, Donaldson SC, Love BC, Knabel SJ, Jayarao BM. Antimicrobial-resistant enteric bacteria from dairy cattle. Appl Environ Microbiol. 2007 Jan;73(1):156-63. Epub 2006 Nov 10.

Straley BA, Donaldson SC, Hedge NV, Sawant AA, Srinivasan V, Oliver SP, Jayarao BM. Public health significance of antimicrobial-resistant gram-negative bacteria in raw bulk tank milk.Foodborne Pathog Dis. 2006 Fall;3(3):222-33. Review.

Jayarao BM, Donaldson SC, Straley BA, Sawant AA, Hegde NV, Brown JL. A survey of foodborne pathogens in bulk tank milk and raw milk consumption among farm families in pennsylvania. J Dairy Sci. 2006 Jul;89(7):2451-8.

Donaldson SC, Straley BA, Hegde NV, Sawant AA, DebRoy C, Jayarao BM. Molecular epidemiology of ceftiofur-resistant Escherichia coli isolates from dairy calves. Appl Environ Microbiol. 2006 Jun;72(6):3940-8.

Hegde NV, Cook ML, Wolfgang DR, Love BC, Maddox CC, Jayarao BM. Dissemination of Salmonella enterica subsp. enterica Serovar Typhimurium var. Copenhagen clonal types through a contract heifer-raising operation. J Clin Microbiol. 2005 Aug;43(8):4208-11.

Sawant AA, Sordillo LM, Jayarao BM. A survey on antibiotic usage in dairy herds in Pennsylvania.J Dairy Sci. 2005 Aug;88(8):2991-9.

Oliver SP, Jayarao BM, Almeida RA. Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodborne Pathog Dis. 2005 Summer;2(2):115-29. Review.

Jayarao BM, Pillai SR, Wolfgang DR, Griswold DR, Rossiter CA, Tewari D, Burns CM, Hutchinson LJ. Evaluation of IS900-PCR assay for detection of Mycobacterium avium subspecies Paratuberculosis infection in cattle using quarter milk and bulk tank milk samples. Foodborne Pathog Dis. 2004 Spring;1(1):17-26.

Jayarao BM, Pillai SR, Sawant AA, Wolfgang DR, Hegde NV. Guidelines for monitoring bulk tank milk somatic cell and bacterial counts. J Dairy Sci. 2004 Oct;87(10):3561-73.

Zhang W, Jayarao BM, Knabel SJ. Multi-virulence-locus sequence typing of Listeria monocytogenes. Appl Environ Microbiol. 2004 Feb;70(2):913-20.

Gillespie BE, Mathew AG, Draughon FA, Jayarao BM, Oliveri SP. Detection of Salmonella enterica somatic groups C1 and E1 by PCR-enzyme-linked immunosorbent assay. J Food Prot. 2003 Dec;66(12):2367-70.

Sharer MV, Su C, Hegde NV, Jayarao BM, Sordillo LM. Differential expression of the lactose transporter gene affects growth of Staphylococcus aureus in milk. J Dairy Sci. 2003 Jul;86(7):2373-81.

Jayarao BM, Wolfgang DR. Bulk-tank milk analysis. A useful tool for improving milk quality and herd udder health. Vet Clin North Am Food Anim Pract. 2003 Mar;19(1):75-92, vi. Review.

Sawant AA, Pillai SR, Jayarao BM. Evaluation of five selective media for isolation of catalase-negative gram-positive cocci from bulk tank milk. J Dairy Sci. 2002 May;85(5):1127-32.

Pillai SR, Jayarao BM. Application of IS900 PCR for detection of Mycobacterium avium subsp. paratuberculosis directly from raw milk. J Dairy Sci. 2002 May;85(5):1052-7.

Contact Information: 115 Henning, Penn State University, University Park, PA 16802. Phone: 814-863-5939; Email: (bmj3@psu.edu) .

9. Wansheng Liu: Professor of Production Animal Genomics. Department of Dairy and Animal Science, The Center for Reproductive Biology and Health,

College of Agriculture, The Pennsylvania State University, 305 Henning, University Park, PA 16802, USA.

Title Of the talk: Decoding the Bovine Y Chromosome.

Abstract:

The bovine genome has been sequenced. As the sequenced DNA was isolated from a cow, the bovine Y chromosome (BTAY) sequence and gene content, structure, order and function are not available. To decode the bovine Y, we have conducted a direct cDNA selection by hybridizing the bovine testis cDNA with the entire BTAY DNA as a probe. The selected cDNA was sequenced using the next generation sequencing technology (Illumina GA2), which resulted in a total of 13 million (M) reads (36 bp). Approximately 5 M reads matched to the BTAY BACs. Assembly of these Y-related reads yielded approximately 2000 contigs with size of >100 bp. After annotation, we identified 14 known Y genes that have orthologs in other mammalian Y chromosomes, and over 50 bovid lineage-specific novel Y genes/transcripts. Expression profiling indicated that the BTAY genes are expressed predominantly or exclusively in testis with sense and antisense mRNAs detected particularly in the late stage of spermatogenesis. Two of the novel lineage-specific Y gene families, ZNF280BY and PRAMEY, were originated from the transposition of a gene block ZNF280B-ZNF280A-PRAME on BTA17 and subsequently amplified on BTAY. The transposed gene families have acquired functional and evolutionary advantages for male spermatogenesis during

the evolution. We developed a pipeline to integrate these known genes and novel transcripts with BTAY-BAC sequences, and generated assemblies of several megabases of DNA sequence for the male-specific region (MSY). The assembled sequence revealed large blocks (30-110 Kb) of directed and inverted repeats with similarity ≥ 99.85%, signifying the genomic complexity of BTAY. We also found that HSFY and ZNF280BY are the most abundant gene families with over a hundred loci on BTAY, which spread over 2/3 of the Y as the main elements of the ampliconic region.

Biographical sketch of Wansheng Liu

EDUCATION:

B.S., 1982, Animal Science, Northwest Agricultural University, China.

M.S., 1987, Animal Breeding and Genetics, Northwest Agricultural University, China. Ph.D., 1993, Animal Breeding and Genetics, Northwest Agricultural University, China. Post-Doctorate, 1996-1999, Department of Genetics, Uppsala University, Sweden. Post-Doctorate, 1999-2003, Department of Animal Science, University of Minnesota.

PROFESSIONAL EXPERIENCE:

Associate Professor (2007-present): Department of Dairy and Animal Science, Pennsylvania State University. Associate Research Professor (2003-2007): Department of Animal Biotechnology, University of Nevada, Reno. Research Associate (1999-2003): Department of Animal Science, University of Minnesota. Researcher & Research Associate (1997-1999), Department of Genetics, Uppsala University, Sweden. Department of Cell and Molecular Biology (CMB), Medical Nobel Institute, Karolinska Institute, Stockholm, Sweden. Department of Medicine, University of California, San Francisco (UCSF). Visiting Scholar (1995-1996): Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden. Associate Professor (1994-1996), Institute of Developmental Biology, Department of Biology, Shandong University, Jinan, Shandong, China.

Associate Professor (1992-1993): Department of Animal Science, Northwest Agricultural University, Shaanxi, China. Lecturer (1987-1991): Department of Animal Science, Northwest Agricultural University, Shaanxi, China. Assistant Professor (1982-1986): Department of Animal Science, Northwest Agricultural University, Shaanxi, China.

RESEARCH INTERESTS:

My research interests are in the areas of animal genetics, structural, functional and comparative genomics, mammalian Y-chromosome and male fertility. My current research projects are: • Functional characterization of the bovine Y-chromosome (BTAY), with a focus on Y gene polymorphisms (haplotype) and male fertility; • Development of a high-resolution radiation hybrid (RH) map for the swine genome using a 12,000-rad whole genome pig-Chinese hamster RH panel; • Molecular mechanism involved in controlling placental efficiency in sheep.

PUBLICATIONS (last 10 years):

Liu, W.-S., Wang, A.-H., Yang, Y., Chang, T.-C., Landrito, E., Yasue, H. (2009) Molecular characterization of the DDX3Y gene and its homologs in cattle. Cytogenetic and Genome Research. (In press)

Porada, C.D., Sanada, C., Long, C.R., Wood, J.A., Desai, J.,Frederick, N., Millsap, L., Bormann, C., Menges, S.L., Hanna, C., Flores-Foxworth, G., Shin, T., Westhusin, M.E., Liu, W.-S., Glimp, H., Zanjani, E.D., Lozier, J.N., Pliska, V., Stranzinger, G., Joerg, H., Kraemer, D.C., Almeida-Porada, G. (2009) Clinical and molecular characterization of a re-established line of sheep exhibiting hemophilia A. Journal of Thrombosis and Haemostasis. (In press)

Liu, W.-S., Yang Y., Chang, T.-C. (2009) Isolation and characterization of male fertility-related genes on the bovine Y chromosome. Biology of Reproduction 81, 671.

Ma, J.-G., Yasue, H., Eyer, K., Hiraiwa, H., Shimogiri, T., Meyers, S.N., Beever, J.E., Schook, L.B., Beattie, C.W. Liu, W.-S. (2009) An integrated RH map of porcine chromosome 10 (SSC10). BMC Genomics 10, 211 doi:10.1186/1471-2164-10-211.

Colletti, E.J., Airey, J.A., Liu, W.-S., Zanjani, E.D., Porada, C.D., Almeida-Porada, G. (2009) Generation of tissue-specific cells by MSC does not require fusion or donor to host mitochondrial/membrane transfer. Stem Cell Research 2, 125–138.

Liu, W.-S., Yasue, H., Eyer, K., Hiraiwa, H., Shimogiri, T., Roelofs, B., Landrito, E., Ekstrand, J. Treat, M., Paes, N., Lemos, M., Griffith, A.C., Davis, D.L., Meyers, S.N., Yerle, M., Milan, D., Beever, J.E., Schook, L.B., Beattie, C.W. (2008) High-resolution comprehensive radiation hybrid maps of the porcine chromosome 2p and 9p compared with the human chromosome 11. Cytogenetic and Genome Research 120 (1-2), 157-163.

Wang, A.-H., Yasue, H., Li, L., Tagashima, M., Ponce de León, F.A., Liu, W.-S. (2008) Molecular charterization of the bovine chromodomain Y-like (CDYL) genes. Animal Genetics 39, 207-216.

Guo, H., Liu, W.-S., Takasuga, A., Eyer, K., Landrito, E., Xu, S.-Z., Gao, X., Ren, H.-Y. (2008) Mapping and association of GAD2 and GIP gene variants with feed intake and carcass traits in beef cattle. Arch. Tierz., Dummerstorf 51 (1), 33-41.

Guo, H., Liu, W.-S., Takasuga, A., Eyer, K., Landrito, E., Xu, S.-Z., Gao, X., Ren, H.-Y. (2008) Mapping, expression, and association study of the bovine PSMC1 gene. Biochemical Genetics 46, DOI 10.1007/s10528-008-9151-0.

Liu, W.-S., Wang, A., Uno, Y., Galtz, D., Beattie, C.W., Ponce de León, F.A. (2007) Genomic structure and transcript variants of the bovine DAZL gene. Cytogenetic and Genome Research 116 (1-2), 65-71.

Liu, W.-S., Ponce de León, F.A. (2007) Mapping of the Bovine Y Chromosome. Electronic Journal of Biology 3 (1), 5-12.

Guo, H., Liu, W.-S., Takasuga, A., Eyer, K., Landrito, E., Xu, S.-Z., Gao, X., Ren, H.-Y. (2007) Characterization and mapping of the bovine FBP1 gene. Asian-Australasian Journal of Animal Sciences 20(9), 1319-1326.

Rink, A., Eyer, K., Roelofs, B., Sharkey-Brockmeier, K.J., Lekhong, S., Karajusuf, E.K., Bang, J., Yerle, M., Milan, D., Liu, W.-S., Beattie, C.W. (2006) A radiation hybrid map of the porcine genome comprising 2035 EST loci. Mamm. Genome 17 (8), 878-885.

Guo, H., Liu, W.-S., Takasuga, A., Eyer, K., Landrito, E., Xu, S.-Z., Gao, X., Ren, H.-Y. (2006) Mapping of the CCK, PSMC2, PSMC4, PSMD1, CPB1 and PSPH genes in cattle. Animal Genetics 37 (1), 73-75.

Guo, H., Li, K., Liu, W.-S., Li, H.-D., Xu, S.-Z., Ren, H,-Y., Gao, X. (2006) An isolation, polymorphism and trait association study of bovine GAD1 and GAL genes. South African Journal of Animal Science 36 (4), 269-274.

Ponce de Leon, F.A. Liu, W.-S., Carpio, C. M. (2006) Understanding the organization of BTAY and male fertility genes. Chromosome Research 14, 219.

Liu, W.-S., Eyer, K., Yasue, H., Roelofs, B., Hiraiwa, H., Shimogiri, T., Landrito, E., Ekstrand, J. Treat, M., Rink, A., Yerle, M., Milan, D., Beattie, C.W. (2005)

A 12,000-rad porcine radiation hybrid (IMNpRH2) panel refines the conserved synteny between SSC12 and HSA17. Genomics 86 (6), 731-738.

Li, G.H., Liu, W.-S., Takasuga, A., Watanabe, T., Carpio, C.M., Rink, A., Sugimoto, Y., Ponce de León, F.A., Beattie, C. W. (2005) Characterization and RH mapping of microsatellites generated from a microdissected BTA20-specific DNA library. Animal Genetics 36 (2), 146-151.

Porada, C.D., Park, P.J., Almeida-Porada, G., Liu, W.-S., Ozturk, F., Glimp, H.A., Zanjani, E.D. (2005) Gestational age of recipient determines pattern and level of transgene expression following in utero retroviral gene transfer. Molecular Therapy 11(2), 284-293.

Liu, W.-S., Ponce de León, F.A. (2004) Assignment of SRY, ANT3 and CSF2RA to the bovine Y-chromosome by FISH and RH mapping. Animal Biotechnology 15, 103-109.

Liu, W.-S., Beattie, C. W., Ponce de León, F.A. (2003) Bovine Y chromosome microsatellite polymorphisms. Cytogenetic and Genome Research 102, 53-58.

Liu, W.-S., Mariani, P., Beattie, C. W., Alexander, L., Ponce de León, F.A. (2002) A radiation hybrid map for the bovine Y chromosome. Mamm.Genome 13, 320-326.

Liu, W.-S., Nordqvist, K., Lau Y-F.C. and Fredga, K. (2001) Characterization of the Xp21-23 region in the wood lemming, a region involved in XY sex reversal. J. Exp Zool (Mol Dev Evol) 290, 551-557.

Liu, W.-S., Fredga, K. (1999) Telomeric (TTAGGG)n sequences are associated with nucleolus organizer regions (NORs) in the wood lemming. Chromosome Research 7, 235-240.

Liu, W.-S., Erikssion, L. and Fredga, K. (1998) XY sex reversal is associated with deletion of Xp21-23 in the wood lemming as revealed by chromosome microdissection and FISH. Chromosome Research 6, 379-383.

Liu, W.-S., Harbitz, I., Gustavsson, I. and Chowdhary, B. P. (1998) Mapping of the pig Erythropoietin gene to chromosome 3p15-p16 and ordering of four subclones by fiber-FISH and DNA-combing. Hereditas 128(1), 77-81.

Liu, W.-S., Soldatov, N. M., Gustavsson, I. and Chowdhary, B. P. (1998) Fiber-FISH analysis of the 3'-terminal region of the human L-type Ca2+ channel alfa-1C subunit gene. Hereditas 129(2), 169-175.

Contact Information: 305 Henning, , University Park, PA 16802. Phone: 814-867-1673: Email: (wl12@psu.edu) .

10. Isabella Cattadori: Professor of Biology, Centre for Infectious Disease

Dynamics CIDD, The Pennsylvania State University, 508 Mueller Lab.,

University Park, PA 16802, USA.

Title of the Talk: Parasite Co-infections, Immunity & Host Heterogeneity- Key Issues for understanding Disease Transmission.

Abstract:

One of the conditions that affect host susceptibility and parasite transmission is the occurrence of multi species infections. Indeed, co-infections contribute to the severity of some of the most serious human infectious diseases including HIV, malaria and tuberculosis. The appreciation of the immunological mechanisms that affect concomitant infections and how they vary between individual hosts is increasingly recognized to be crucial for the understanding of the epidemiology of co-infections. By linking within-host immunological processes to between-hosts dynamics of infection it is possible to explain the conditions that allow for the invasion and survival of a parasite within a host and the consequences of host mediated responses to parasite transmission. I shall present results on the dynamics of co-infection based on two gastrointestinal nematodes and a bacterium of the respiratory tract, using the rabbit as a model system. I shall show how the host acquired immune response modulates these multi species infections by affecting bacteria replication and nematode development and fecundity. I shall highlight how the intensity and kinetic of the immune response changes with species composition. Finally I shall present evidence of how acquired immunity modulates bacteria and nematode shedding and discuss the relevance of these processes for the persistence of chronic infections.

Biographical Sketch of Dr. Isabella M. Cattadori:

Position 2009- Assistant Professor, Dept. Biology, Penn State University, PA

2006/08 Royal Society University Fellow, Fac. Veterinary Medicine, University of Glasgow (UK) & Research Associate, Dept. Biology, Penn State University, PA

Education and Qualifications 1999 Ph.D (Biological Sciences) University of Stirling (UK) 1990 BSc (= Hons, First Class) (Natural Sciences) University of Milan (Italy)

Fellowships, Awards & Honorary positions 2009- Present. Visiting Fellow Faculty of Vet Medicine, Univ Glasgow (UK)

2006/08 Royal Society University Research Fellowship, Univ Glasgow (UK) 2000/02 European Union Marie Curie Individual Fellowship, Univ Stirling (UK) 1994/96 Graduate Research Fellowship, Centro di Ecologia Alpina, CEA (Italy) 1992/94 Graduate Res Fellow, Istituto Nazionale Fauna Selvatica, INFS (Italy) Memberships

British Ecological Society (1997-present); Ecological Society of America

(2004-present)

AAAS (2005-present); Society of Nematologists (2006).

Publications

32 peer reviewed publications

4 book chapters

Contact Information: 208 MUELLER Laboratory, UNIVERSITY PARK, PA

16802. Phone: 814 865 9594 Fax: 814 865 9131, E-mail: imc3@psu.edu;

Website: http://www.cidd.psu.edu/people/bio_cattadori.html.

11. Troy Ott: Ph.D., PAS, Associate Professor of Reproductive Biology, Department of Dairy and Animal Science, Department of Veterinary and Biomedical Sciences, Center for Reproductive Biology and Health, Pennsylvania State University, University Park, PA 16802, USA.

Title of the Talk: Early Detection of Viral Infections Improves Biosecurity in Animal Agriculture.

Abstract:

Viral diseases cause substantial morbidity and mortality in animal production systems. In addition, several high profile viruses have caused economic devastation to regional animal production systems in recent years. Furthermore, the list of OIE reportable diseases is dominated by virus-caused diseases. A number of viral diseases are also zoonotic, creating concern about disease transmission to humans. Animal movement within and between States and Nations typically requires a Certificate of Veterinary Inspection which certifies the “general healthy appearance” of the animal. In addition, some locales require specific testing for regional or national diseases of concern. Of course, the general veterinary recommendation is to quarantine new arrivals so diseases in the early stages of infection can be detected before animals are mixed in with the herd/flock. Because of the large number of potential viral agents and the fact the many diagnostic tests provide only retrospective information (presence of Antibodies) regarding infection status, there is a significant unmet need for a broadly sensitive, rapid diagnostic to detect viral infections in asymptomatic animals. The presentation will provide background on a recently patented (US) technology for the early detection of viral infections in asymptomatic animals. Availability of a broadly sensitive viral diagnostic will facilitate the rapid diagnosis of virus-caused diseases, reduce their spread, and may help reduce the unnecessary use of antibiotics in animal agriculture. Finally, such a diagnostic may provide a critical first line of defense in emerging, or bioterrorist engineered virus disease detection and control.

Biographical Sketch of Troy Ott:

Dr. Troy L. Ott is a reproductive biologist in the Dairy and Animal Science Department at Penn State. He holds a courtesy appointment in the Department of Veterinary and Biomedical Sciences. Dr. Ott received his B.S. in Animal

Production from Penn State. He completed his Master of Science and Doctorate degrees specializing in reproductive biology, at Auburn University and the University of Florida, respectively. He spent 6 years as a Scientist in the Center for Animal Biotechnology at Texas A&M University before joining the faculty at the University of Idaho, Department of Animal and Veterinary Science. He returned to Penn State in

2006 as part of the Reproductive Biology Initiative that has hired 7 reproductive biologists between the Dairy and Animal Science and Poultry Science departments during the last three years. Dr. Ott’s research focuses on the physiology of early pregnancy with a primary focus on the signaling between the conceptus and uterus during this time. His current work includes studying the mucosal immune system and maternal immune responses to conceptus signaling. He is working to develop novel technologies to aid in managing reproduction and animal health, with a particular focus on the dairy industry. Dr. Ott has published over 75 scientific papers and 8 book chapters. His research has been funded by the USDA and NIH and he holds 4 patents.

Curriculum Vita:

Education /Training:

B. S: The Pennsylvania State University. 1980-1984. Animal science.

M. S. Auburn University. 1985-1888. Reproductive Physiology.

Ph. D. University of Florida. 1988-1992. Reproductive Physiology.

Research scientist. Texas A & M University. Reproductive Physiology.

Professional Experience:

1992-96 Assistant Research Scientist, Center for Animal Biotechnology, Institute of Biosciences and Technology, Texas A&M University, College Station, TX.

1996-98 Research Scientist, Center for Animal Biotechnology, Institute of Biosciences and Technology, Texas A&M University, College Station, TX.

1998-2004 Assistant Professor, Department of Animal and Veterinary Science, Center for Reproductive Biology, University of Idaho, Moscow, ID.

2004-2006 Associate Professor, Department of Animal and Veterinary Science, Center for Reproductive Biology, University of Idaho, Moscow, ID.

2006-present Associate Professor, Department of Dairy & Animal Science, Department of Veterinary and Biomedical Science, Center for Reproductive Biology and Health, Pennsylvania State University, University Park, PA.

Current Professional Societies and Activities:

Member, Society for the Study of Reproduction, American Society of Animal Science, American Society of Reproductive Immunology, American Association for the Advancement of Science.

Ad-hoc review: Biology of Reproduction, American Journal of Reproductive Immunology, Journal of Endocrinology, Journal of Animal Science, Reproduction, Fertility and Development, Domestic Animal Endocrinology, Theriogenology. Chair, LAC, 2010 American Society for Reproductive Immunology annual meeting, Pittsburgh, PA.

Relevant Publications:

Gifford, C.A., Assiri, A.M., Satterfield, M.C., Spencer, T.E. and Ott, T.L. (2008). Receptor transporting protein-4 (RTP-4), in endometrium, ovary and peripheral blood leukocytes of pregnant and cyclic ewes. Biology of Reproduction 79: 518-524.

Stevenson, J.L., Dalton, J.C., Ott, T.L., Racicot, K.E. and Chebel, R.C. (2007). Correlation between reproductive status and steady-state mRNA levels of the Myxovirus resistance gene, MX2, in peripheral blood leukocytes of dairy heifers. Journal of Animal Science. 85(9):2163-72.

Ocón-Grove, O.M, Cooke, F.N.T., Alvarez, I.M., Johnson, S.E., Ott, T.L. and Ealy, A.D. (2007). Ovine Endometrial and Conceptus Expression of Fibroblast Growth Factor (FGF)-2 and FGF Receptors during Early Pregnancy. Domestic Animal Endocrinology. 34(2):135-45.

Ferens, W.A., Halver, M., Gustin, K.E., Ott, T.L., Hovde, C.J. (2007) Differential sensitivity of viruses to the antiviral activity of Shiga toxin 1 A subunit. Virus Research 125: 104-108.

Assiri, A.M. and Ott, T.L. (2007). Cloning and characterizing of the ovine Mx1 gene promoter/enhancer region. Developmental and Comparative Immunology 31(8):847-57.

Gifford, C.A., Racicot, K., Clark, D.S., Austin, K.J., Hansen, T.R., Lucy, M.C., Davies, C.J., Ott, T.L. (2007). Regulation of Interferon Stimulated Genes in Peripheral Blood Leukocytes in Pregnant and Bred, Non-pregnant Dairy Cows. Journal of Dairy Science 90(1): 274-280.

Toyokawa, K., Carling, S.J. and Ott, T.L. (2007). Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): Ovine Mx1 is secreted by endometrial epithelial cells via an “unconventional” secretory pathway. American Journal of Reproductive Immunology 57:13-22.

Toyokawa, K., Leite, F. and Ott, T.L. (2007). Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): II. The oMx1 protein regulates secretion in an ovine glandular epithelial cell line. American Journal of Reproductive Immunology 57:23-33.

Williams, G.A., Ott, T.L., Michal, J.J., Gaskins, C.T., Wright, R.W. and Jiang, Z. (2007) Development of a novel model for mapping the genetic complexity of cryptorchidism in sheep and initial evidence for association of INSL3 with the defect. Animal Genetics 38(2):189-91.

Garcia, M.D., Michal, J.J., Gaskins, C.T., Reeves, J.J., Ott, T.L., Liu, Y. and Jiang, Z. (2006) Significant associations of the calpastatin gene with fertility and longevity in dairy cattle. Animal Genetics 37:304-305.

Hostetler, C.E., Michael, J., Robison, M.R., Ott, T.L. and Kincaid, R.L. (2006). Effect of selenium intake and fetal age on mRNA expression of two selenoproteins in porcine fetal and maternal liver. Journal of Animal Science 84:2382.

Hartt, L., Carling, S.J., Joyce, M.M, Johnson, G.A., Vanderwall, D.K. and Ott, T.L. (2005). Temporal and spatial associations of oestrogen receptor alpha and progesterone receptor expression in the endometrium of pregnant and non-pregnant mares. Reproduction, 130: 1-11.

Yankey, S.J., Hicks, B.A., Carnahan, K.G., Assiri, A., Sinor, S.J., Kodali, K., Stellflug, J.N. and Ott, T.L. (2001). Expression of the antiviral protein Mx in peripheral blood mononuclear cells of pregnant and bred, non-pregnant ewes. Journal of Endocrinology 170, R7-R11.

Singh B., Ott, T.L., Bazer, F.W., and de la Concha-Bermejillo, A. (2001). Phenotypic and Ultrastructural Characteristics of Bronchoalveolar Lavage Cells of Lentivirus-Infected Lambs Treated with Recombinant Ovine Interferon-tau. Journal of Interferon and Cytokine Research 21(9):677-686.

Ott, T.L. (2000). Interferon-tau: paracrine mediator of conceptus-maternal dialogue in ruminants. Annales, Universitatis Mariae Curie-Sklodowska. Sectio DD, Medicina Veterinaria. Vol. LV/B, pgs 1-5.

Juste, R.A., Ott, T.L., Kwang, J., Bazer, F.W. and de la Concha-Bermejillo, A. (2000). Effects of recombinant ovine interferon- on ovine lentivirus replication and progression of disease. Journal of General Virology 81(2): 525-532.

Kim, H-T, Stoica, G., Bazer, F.W. and Ott, T.L. (2000). Interferon-tau (IFN)-induced apoptosis in ovine hepatocytes. Hepatology 31:1275-1284.

Tuo, W., Ott, T.L., Liu, S-H. and Bazer, F.W. (1999). Intrauterine infusion of bacterial lipopolysaccharide (LPS) prior to mating has no adverse effects on fertility, fetal survival and fetal development. Journal of Reproductive Immunology 42:31-39.

Ing, N.H. and Ott, T.L. (1999). Estradiol up-regulates estrogen receptor messenger RNA by increasing its stability. Biology of Reproduction 60:134-139.

Ott, T.L., Yin, J., Wiley, A.A., Kim, H.-T., Gerami-Naini, B., Spencer, T.E., Bartol, F.F., Burghardt, R.C. and Bazer, F.W. (1998). Effects of the estrous cycle and early pregnancy on uterine expression of Mx protein in sheep (Ovis aries). Biology of Reproduction 59:784-794.

Singh, B., Ott, T.L., Bazer, F.W. and De La Concha-Bermejillo, A. (1998). Structural responses of pulmonary intravascular macrophages in lentivirus-infected and/or recombinant ovine interferon--treated lambs. The Anatomical Record 251:472-485.

Pontzer, C.H., Yamamoto, J.K., Bazer, F.W., Ott, T.L. and Johnson, H.M. (1997). Potent Anti-Feline Immunodeficiency Virus and Anti-Human Immunodeficiency Virus Effect of IFN-.

The Journal of Immunology 158:4351-4357.

Juste, R.A., Ott, T.L., Kwang, J., Bazer, F.W. and De La Concha-Bermejillo, A. (1996). Effects of recombinant interferon-tau on ovine lentivirus replication. Journal of Interferon and Cytokine Research 6:989-994.

Newton, G.R., Ott, T.L., Woldesenbet, S., Shelton, A.H. and Bazer, F.W. (1996). Biochemical and immunological properties of related small ruminant trophoblast interferons. Theriogenology 46:703-716.

Zhu, D., Ott, T.L., and Bazer, F.W. (1996). An enzyme-linked immunosorbent assay for ovine interferon-τ (oIFNτ). Journal of Interferon and Cytokine Research 16:147-150.

Spencer, T.E., Mirando, M.A., Mayes, J.S., Watson, G.H., Ott, T.L. and Bazer, F.W. (1996). Effects of interferon-tau and progesterone on oestrogen stimulated expression of receptors for oestrogen, progesterone and oxytocin in the endometrium of ovariectomized ewes. Reproduction Fertility and Development 8:843-853.

Pontzer, C.H., Ott, T.L., Bazer, F.W. and Johnson, H.J. (1994). Structure/Function studies with interferon tau: Evidence for multiple active sites. Journal of Interferon Research 14:133-141.

Tuo, W., Ott, T.L. and Bazer, F.W. (1993). Natural killer cell activity of lymphocytes exposed to ovine, type I, trophoblast interferon. American Journal of Reproductive Immunology 29:26-34.

Pontzer, C.H., Ott, T.L., Bazer, F.W. and Johnson, H.M. (1990). Localization of an antiviral site on the pregnancy recognition hormone, ovine trophoblast protein 1. Proceedings of the National Academy of Science, USA 87:5945-5949.

12. Avery August: Professor of Immunology, Molecular Virologist with expertise in animal models for human medicine, Center for Molecular Immunology & Infectious Disease, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA 16802, USA.

Title of the Talk: Animal models of human respiratory disease. Abstract:

Animal models of human disease have played an important role in understanding the pathology and mechanisms of these diseases. In

particular, murine models have made significant contributions, due to the wealth of available reagents and the ability to fairly easily genetically manipulate these animals. However, whether these models are exact parallels of human disease varies dependent on the disease. I will discuss our recent efforts in modeling allergic asthma in mouse models of allergic inflammation, and address several critical findings that emerge from these studies and their applicability to the human disease. Biographical Sketch of Dr. Avery August:

Education and Training:

1987-1988--Graduate Research Assistant, Dept. of Chemistry and Biochemistry, California State University, Los Angeles, CA. Advisor: Prof. Phoebe Dea.

1988-1994--Graduate Research Assistant, Immunology Graduate Program, Weill Graduate School of Medical Sciences, Cornell University and Memorial Sloan Kettering Cancer Center, New York, NY. Advisor: Dr. Bo Dupont.

1994-1997--Post-Doctoral Fellow, Laboratory of Molecular Oncology, The Rockefeller University, New York, NY. Advisor: Dr. Hidesaburo Hanafusa.

1997-1999--Scientist, Immunology Drug Discovery, The R.W. Johnson Pharmaceutical Research Institute, Raritan, NJ.

1999-2004-- Assistant Professor of Immunology, Department of Veterinary and Biomedical

Science, The Pennsylvania State University, University Park, PA.

2004-2008-- Associate Professor of Immunology (with Tenure), Department of Veterinary and Biomedical Science, The Pennsylvania State University, University Park, PA.

2004-Present--Chair, Molecular Medicine Graduate Program, The Pennsylvania State

University, University Park, PA.

2007-Present--Director, Center for Molecular Immunology & Infectious Disease, The Pennsylvania State University, University Park, PA.

2008-Present-- Professor of Immunology (with Tenure), Department of Veterinary and Biomedical Science, The Pennsylvania State University, University Park, PA.

Awards and Honors 1987 Dean's Honor List, California State University, Los Angeles 1987-1988 RIMI Graduate Scholar 1991 NIGMS Minority Pre-Doctoral Fellowship 1994 ASHI Scholar in Basic Sciences 1995-1997 NSF Minority Post-Doctoral Fellow 2000 Johnson & Johnson Focused Giving Program Awardee 2000 Leukemia Research Foundation Investigator.

2002-2009 NIH Study Section Review Panel, CSR SEP-CSR Special Emphasis Panels, (F32

pre-doctoral fellowship programs).

2002, 2003 Ad Hoc member, NIH Study Section Review Panel, Lung Biology and Pathology (LBPA). 2004-2009 Permanent Member, NIH Study Section Review Panel, Lung Cell and Molecular Immunobiology (LCMI). 2004-Present Editorial Board, The International Journal of Biochem & Cell Biology. 2006-2011 Board of Scientific Counselors, National Institute on Aging, NIH 2008-2011 Chair, Board of Scientific Counselors, National Institute on Aging, NIH 2007 Community of Scholars Teaching Award Recipient 2007-2008 AAFA Investigator Grant Award Recipient

B. PUBLICATIONS (Selected from a total of 72 published, in press or submitted): 1. A. August, S. Gibson, Y. Kawakami, T. Kawakami, G.B. Mills and B. Dupont. “CD28 is associated with and induces the immediate tyrosine phosphorylation and activation of the Tec family kinase ITK/EMT in the human Jurkat leukemic cell line.” (1994) Proc. Natl. Acad. Sci. USA. 91:9347. 2. S. Gibson*, A. August*, D. Branch, B. Dupont and G.B. Mills. “Functional LCK is required for optimal CD28-mediated activation of the TEC family tyrosine kinase EMT/ITK.” (1996) J. Biol. Chem., 271: 7079. (* equal contributors). 3. S. Gibson, A. August, Y. Kawakami, T. Kawakami, B. Dupont, G.B. Mills. “The EMT/ITK tyrosine kinase is activated during T cell receptor signaling: LCK is required for optimal activation of EMT.” (1996) J. Immunol., 156:2716. 4. A. August and B. Dupont. “Association of MAPK and the ζ chain of the TCR to the SH2,3 domain of Lck: differential regulation by TcR crosslinking.” (1996) J. Biol. Chem., 271:10054. 5. C. Zong, R. Yan, A. August, J.E. Darnell, Jr., J.E., and H. Hanafusa. “Unique signal transduction of Eyk: constitutive stimulation of the JAK-STAT pathway by an oncogenic receptor-type tyrosine kinase.” (1996) Embo. J., 15:4515. 6. A.N.A. Monteiro*, A. August*, and H. Hanafusa. “A transcriptional activation function for BRCA1 C-terminal region.” (1996) Proc. Natl. Acad. Sci. 93: 13595. (*equal contributors). 7. P.D. King, J.M.C. Teng, A. Sadra, X.-R. Liu, A. Han, A. Selvakumar, A. August, and B. Dupont. “Analysis of CD28 cytoplasmic tail residues as regulators and substrates for protein tyrosine kinases, EMT and LCK.” (1997) J. Immunol. 158: 581. 8. A. August, A. Sadra, B. Dupont and H. Hanafusa. “Src induced activation of Inducible T cell Kinase (ITK) requires PI3 kinase activity and the Pleckstrin Homology domain of inducible T cell kinase.” (1997) Proc. Natl. Acad. Sci. 94: 11227. 9. T. Ouchi, A.N.A. Monteiro, A. August, S.A. Aaronson, and H. Hanafusa. “BRCA1 regulates p53-dependent gene expression.” (1998) Proc. Natl. Acad. Sci 95: 2302. 10. M.L. Woods, W.J. Kivens, M.A. Adelsman, Y. Qui, A. August and Y. Shimizu. “Distinct functions for Phosphatidylinositol 3-kinase and p56lck in the regulation of β1 Integrin function by the tyrosine kinase ITK.” (2001) Embo. J. 20:1232. 11. J. Cook, A. August and A. Henderson. “Recruitment of Phosphatidylinositol-3-kinase to CD28 inhibits HIV transcription by a Tat-dependent mechanism.” (2002) J. Immunol. 169:254-260. 12. C. Mueller and A. August. “Attenuation of Immunological symptoms of Allergic Asthma in mice lacking ITK.” (2003) J. Immunol. 170:5056. 13. S. Hao, T. Kurosaki and A. August. “Differential regulation of NFAT and SRF by the B cell

Receptor via a PLCγ/Ca2+ dependent pathway” (2003) Embo. J. 22:4166. 14. J.A. Cook, L. Albacker, A. August and A.J. Henderson. “CD28-dependent HIV-1 Transcription is associated with Vav, Rac, and NF-κB Activation” (2003) J. Biol. Chem. 278:35812. 15. A.M. Fischer, J.C. Mercer, A. Iyer, M.J. Ragin and A. August. “Regulation of CXCR4 mediated migration by the Tec family tyrosine kinase ITK” (2004) J. Biol. Chem. 279:29816-20. *Recommended by the Faculty of 1000. 16. A. Wittke, V. Weaver, B.D. Mahon, A. August and T. Cantorna. “Vitamin D receptor deficient mice fail to develop experimental allergic asthma” (2004) J. Immunol. 173:3432-6. 17. S. Hao, and A. August. “Actin depolymerization transduces the strength of B cell Receptor stimulation.” (2005) Mol. Biol. Cell 16:2275-2284. 18. T. J. Ferrara¶, C. Mueller¶, N. Sahu¶, A. Ben-Jebria, and A. August. “Reduced airway hyperresponsiveness and Tracheal responses during allergic asthma in mice lacking tyrosine kinase ITK.” (2006) J. Allergy & Clin. Immunol. 117:780-6. (¶equal contributors). 19. Q. Qi, N. Sahu and A. August. “Tec kinase Itk forms membrane clusters specifically in the vicinity of recruiting receptors”. (2007) J. Biol. Chem 281, 38529-38534. 20. Q. Qi and A. August. “Keeping the (kinase) party going: SLP-76 and ITK dance to the beat” (2007) Science STKE. 396:pe39. 21. J. Hu, N. Sahu, E. Walsh and A. August. “Memory phenotype CD8+ T-cells with innate function selectively develop in the absence of active Itk”. (2007) Eur. J. Immunol. 37:2892-2899. 22. M. Sun, S.M. Fuentes, K. Timani, D. Sun, C. Murphy, Y. Lin, A. August, M. N. Teng, and B. He. “Akt plays a critical role in replication of non-segmented negative stranded RNA viruses.” (2008) J. Virol. 82:105-14. 23. . N. Sahu, C. Mueller, A. Fischer, and A. August. “Differential sensitivity to Itk kinase signals for T helper 2 cytokine production and chemokine mediated migration.” (2008) J. Immunol. 180(6):3833-8. 24. J. Hu and A. August. “Naïve and Memory phenotype CD4+ T-cells have different requirements for active Itk for their development” (2008) J. Immunol. 180:6544-52. 25. A. Iyer and A. August. “The Tec kinase Itk differentially controls mast cell responses.” (2008) J. Immunol. 180:7869-77. 26. E.R. Walsh, N. Sahu, J. Kearley, E. Benjamin, B.H. Kang, A. Humbles and A. August. “Strain specific requirement for eosinophils in the recruitment of T cells to the lung during the development of allergic asthma.” (2008) J. Exp. Med. 205:1285-92. 27. . J. A. Readinger*, G. Schiralli*, J-K. Jiang, C. Thomas, A. August, A. J. Henderson, and P. L. Schwartzberg. “Selective Targeting of ITK blocks multiple steps of HIV Replication” (2008) Proc. Natl. Acad. Sc. (USA) 105:6684-89. 28. A. B. Strasner, T. Doman, D. Key, A. August, A. J. Henderson. “The Src Kinase Lck is required for Efficient Assembly of HIV-1 at the Plasma Membrane” (2008) J. Immunol. 181:3706-13. 29. N. Sahu*, A. M. Venegas*, D. Jankovic, W. Mitzner, J. Cannons, C. Sommers, P. Love, A. Sher, P. L. Schwartzberg*, and A. August*. “Tec kinases, Txk and Itk, differentially regulate Th1 and Th2 responses by selective expression, not function.” (2008) J. Immunol. 181:6125-31. 30. D. Bruce, A. August, M. A. McDowell, and M. T. Cantorna. “Vitamin D receptor is not required for primary or secondary immune responses to Listeria monocytogenes” (2009) Int. Immunol 21:113-22. 31. . Q. Qi, M. Xia, A. Iyer, E. Hicks, N. Xiong, and A. August. “Enhanced development of CD4+ γδ T cells in the absence of Itk results in elevated IgE production” (2009) Blood 114:564-71. *Recommended by the Faculty of 1000. 32. . N. Sahu and A. August. “Itk inhibitors in inflammation and immune-mediated disorders” (2009) Curr. Top. Med. Chem. 9:690-703. 33. A. August. “ITK finds another (dance) partner….TFII-I” (2009) Eur. J. Immunol. 39:2354-7. 34. . Q. Qi and A. August. “The Tec family kinase Itk exists as a folded monomer in vivo.” (2009) J.

Biol. Chem. 284:29882–29892. 35. J. Gomez-Rodrigues, N. Sahu, R. Handon, T.S. Davidson, S.M. Anderson, M.R. Kirby, A. August, and P.L. Schwartzberg. “Differential Expression of Interleukin-17A and -17F Is Coupled to T Cell Receptor Signaling via Inducible T Cell Kinase.” (2009) Immunity 31:587-97. 36. E. R. Walsh and A. August. “Eosinophils and allergic airways disease: There is more to the story” (2009) Trends in Immunology In press. 37. . J. Mercer*, Q. Qi*, L. F. Mottram, A. Iyer, D. Bruce, M.-K. Law, J. L. Morales, H. Shirai, H. Yamazaki, B. R. Peterson and A. August. “Chemico-genetic identification of the actin binding protein Drebrin as a regulator of extracellular calcium uptake” Submitted. 38. . Q. Qi, M. Xia, S. Yu, M. Cantorna, and A. August. “iNKT cell development is partially independent of the kinase domain of Itk” Submitted 39. P. Khanna, T. Yunkunis, H. S. Muddana, H.-H. Peng, A. August, and C. Dong. “Regulation of VE-cadherin Disassembly via p38 MAP Kinase phosphorylation during Melanoma Transendothelial Migration.” Submitted. 40. J. Hu*, Q. Qi*, and A. August. “Itk derived signals regulate the expression of Th-POK and controls the development of CD4+ T cells” Submitted.

Research Support Ongoing R01-AI051626 August (PI) 2/1/08-1/31/2013 NIH/NIAID Tyrosine Kinase ITK in Experimental Allergic Asthma This project involves analysis of ITK in T helper cell differentiation and the development of Allergic Asthma induction in transgenic and knock out mice. 3R01AI051626-07S1 August (PI) 7/14/09-9/30/2010 NIH/NIAID Tyrosine Kinase ITK in Experimental Allergic Asthma This is a supplement to support 2 high school students, 2 undergraduate students and a high school teacher on the project analyzing ITK in T helper cell differentiation and the development of Allergic Asthma induction in transgenic and knock out mice. 3R01AI051626-07S2 August (PI) 9/23/09-9/22/2012 NIH/NIAID Tyrosine Kinase ITK in Experimental Allergic Asthma This is a minority supplement to support post-doctoral fellow Dr. J. Luis Morales on the project analyzing ITK in T helper cell differentiation and the development of Allergic Asthma induction in transgenic and knock out mice. R01-AI065566 August (PI) 7/01/05-12/31/11 NIH/NIAID ITK, mast cells and allergic airway responses This project involves analysis of the role ITK in modulating mast cells during allergic airway responses. R25GM078675 August (PI) 9/1/06-8/30/10 (NCE) NIH/NIGMS Alcorn State University: Penn State University Bridges to the Doctorate Program. This is a program to enhance the research experience of Masters students at Alcorn State University and transition them onto PhD program at Penn State University. R01-AI073955 August (PI) 6/1/08-5/31/13 NIH/NIAID Eosinophils and Allergic Airway Responses This project involves analysis of the role of eosinophils in regulating T cell functions during the development of Allergic Asthma. 3R01AI073955-02S1 August (PI) 9/17/09-8/31/2011 NIH/NIAID Eosinophils and Allergic Airway Responses This is a minority supplement to support graduate student Kindra Stokes on the project involving analysis of the role of eosinophils in regulating T cell functions during the development of Allergic Asthma.

Investigator Award August (PI) 9/1/09-8/31/10 Penn State Clinical Translational Institute T cells, macrophages and bacteria in asthma This project involves analysis of the role of eosinophils in T cell, macrophages and select bacteria in patients with Asthma and COPD. Contact Information: 115 Henning, University Park, PA 16802. Phone: 814 863 3539; Email: (axa45@psu.edu) . 13. Biao He: Associate Professor and GRA-Distinguished Investigator.

Department of Infectious Diseases, University of Georgia, Athens, GA

Title of the Talk: A novel platform for developing vaccine against H5N1

Abstract:

Influenza A virus causes significant morbidity and mortality each year. On the horizon is another potentially pandemic strain of influenza, H5N1. This avian influenza virus has most notably emerged in Southeast Asia and threatens to become the next pandemic. Currently, the only FDA-approved vaccine against H5N1 has serious limitations, particularly as it has to be given twice and requires substantial higher concentrations of the vaccine to achieve a moderate level of efficacy compared to conventional influenza vaccines. Conventional vaccines utilizing the HA and NA of H5N1 viruses have been poorly immunogenic and have safety and production issues. A live-attenuated H5N1 vaccine has been generated by reverse genetics, but the risk of generating a reassortant prohibits use of this vaccine in most instances. Inactivated virus vaccines have also been derived by reverse genetics and produced in large quantities, but preliminary results from NIAID clinical trials suggest that efficacy will require both multiple immunizations and 6 times the standard influenza virus antigen dose while only providing protection in a subset (~50%) of vaccinated individuals. Thus, there is a rationale need for new vaccine strategies that provide increased immunogenicity and safety.

Parainfluenza virus 5 (PIV5), a paramyxovirus, causes kennel cough in dogs, but is not known to cause any illness in humans. PIV5 can be produced in high titers in

Vero cells, which are WHO-approved for vaccine production. Previously, we have reported that a single dosage of 104 PFU of a live recombinant PIV5 expressing a HA gene from the H3 subtype provided immunity against influenza A virus subtype 3 infection in mice. In this work, we expressed H5N1 antigen using PIV5 and found that the recombinant PIV5 expressing H5 effective in protecting mice against H5N1 challenge.

Biographical Sketch of Bioa He

BIOGRAPHICAL SKETCH

NAME Biao He

POSITION TITLE Associate Professor of Virology

EDUCATION/TRAINING

INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY

Wuhan University, Wuhan, China B.S. 1987 Virology State University of New York Downstate Medical

Brooklyn, NY Ph.D. 1996 Molecular Biology Howard Hughes Medical Institute Northwestern University, Evanston, IL 1996-2001 Molecular Virology

A. POSITIONS:

2009-present: Associate Professor and GRA-Distinguished Investigator

Department of Infectious Diseases, University of Georgia, Athens, GA

2007-2009: Associate Professor, Pennsylvania State University, University Park, PA

2001-2007: Assistant Professor, Pennsylvania State University, University Park, PA

1996-2001: Associate, Howard Hughes Medical Institute

Department of Biochemistry, Molecular Biology and Cell Biology

Northwestern University, Evanston, IL

1990-1996: Graduate Student (Ph.D. in Molecular Biology)

Department of Microbiology & Immunology

State University of New York Health Science Center at Brooklyn

1987-1989: Researcher, National Institute of Vaccine and Serum, Beijing, P. R. China

1983-1987: Undergraduate Student (B.S. in Virology), Department of Virology &

Molecular Biology, Wuhan University, P. R. China

B. PUBLICATIONS:

1. He, B., Durbin, R. K. and McAllister, W. T. (1995) Phage RNA polymerase expression vectors that allow efficient expression of cloned genes in both prokaryotic and eukaryotic cells Gene 164, 75-79.

2. He, B., Rong, M., Lyakhov, D., Gartenstein, H., Diaz, G., Castagna, R., McAllister, W. T. and Durbin, R. K. (1997) Rapid mutagenesis and purification of phage RNA polymerases Protein Expression and Purification 9, 142-151.

3. He, B., Rong, M., Durbin, R. K. and McAllister, W.T. (1997) A mutant T7 RNA polymerase that is defective in RNA binding and blocked in the early stages of transcription J. Mol. Biol. 265, 275-288.

4. Lyakhov, D., He, B., Zhang, X., Studier, F. W., Dunn, J. J. and McAllister, W. T. (1997) Mutant T7 RNA polymerases with altered termination properties J. Mol. Biol. 269, 28-40.

5. Lyakhov, D., He, B., Zhang, X., Studier, F. W., Dunn, J. J. and McAllister, W. T. (1998) Pausing and termination by bacteriophage T7 RNA polymerase J. Mol. Biol. 280, 201-213.

6. Rong, M., He, B., McAllister, W. T. and Durbin, R. K. (1998) Promoter specificity determinants of T7 RNA polymerase Proc. Natl. Acad. Sci. U.S.A. 95, 515-519.

7. He, B., Paterson, R. G., Ward, C. D. and Lamb, R. A. (1997) Recovery of infectious SV5 from cloned DNA and expression of a foreign gene Virology 237, 249-260.

8. He, B., Kukarin, A. Temiakov, D., Chin-Bow, S. T., Lyakhov, D., Rong, M., Durbin, R. K. and McAllister, W. T. (1998) Characterization of an unusual, sequence-specific termination signal for T7 RNA polymerase J. Biol. Chem. 273, 18802-18811.

9. He, B., Leser, G. P., Paterson, R. G., and Lamb, R. A. (1998) The Paramyxovirus SV5 small hydrophobic (SH) protein is not essential for virus growth in tissue culture cells Virology 250, 30-40.

10. He, B., and Lamb, R. A. (1999) Effect of inserting the paramyxovirus SV5 gene junctions at the HN/L gene junction: Analysis of accumulation of mRNAs transcribed from rescued viable viruses J. Virol. 73, 6228-6234.

11. Pekosz, A., He, B., and Lamb, R. A. (1999) Reverse genetics of negative strand RNA viruses: Closing the circle Proc. Natl. Acad. Sci. U.S.A. 96, 8804-8806.

12. Schmitt, A.P., He, B., and Lamb, R. A. (1999) Involvement of the cytoplasmic domain of the simian virus 5 hemagglutin-neuramindase protein in paramyxovirus assembly J. Virol. 73, 8703-8712

13. Young, D.F., Chatziandreou, N., He, B., Goodbourn, S., Lamb, R.A. and Randall, R.E. (2001) A single amino acid substitution in the V protein of SV5 differentiates its ability to block IFN signaling in human and murine cells J. Virol. 75, 3363-3370.

14. He, B., Lin, G. Y., Durbin, J. E., Durbin, R. D., and Lamb, R. A. (2001) The SH integral membrane protein of the paramyxovirus SV5 is required to block apoptosis in MDBK cells J. Virol. 75, 4068-79

15. Poole, E., He, B., Lamb, R.A., Randall, R.E. and Goodbourn, S. (2002) The V proteins of simian virus 5 and other paramyxoviruses inhibit induction of interferon-β. Virology, 303, 33–46

16. He, B., Paterson, R.G., Stock, N., Durbin, J.E., Durbin, R. K., Goodbourn, S., Randall, R.E. and Lamb, R.A. (2002) Recovery of paramyxovirus simian virus 5 with a V protein lacking the conserved cysteine-rich domain: The multifunctional V protein blocks both interferon-β induction and interferon signaling. Virology, 303, 15–32

17. Lin, Y., Bright, A.C., Rothermel, T.A. and He, B. (2003) Induction of apoptosis by paramyxovirus simian virus 5 lacking small hydrophobic gene. J. Virol. 77(6): 3371-83

18. Sun, M., Rothermel, T.A., Shuman, L., Aligo, J.A., Xu, S., Lin, Y., Lamb, R. A. and He, B. (2004) Conserved cysteine-rich domain of paramyxovirus simian virus 5 V protein plays an important role in blocking apoptosis J. Virol. 78:5068-78

19. Lin, Y., Horvath, F.J., Wilson, R.L. and He, B. (2005) The role of simian virus 5 V protein on viral RNA synthesis Virology 338(2): 270-80

20. Wilson, R.L., Fuentes, S. and He, B. (2006) Functions of Small Hydrophobic Proteins of Paramyxovirus J. Virol. 80 (4): 1700-9

21. Tompkins, S.M., Lin, Y., Leser, G.P., Kramer, K.A., Haas, D.L., Durbin, R.K., Durbin, J.E., Tripp, R., Lamb, R.A. and He, B. (2007) Recombinant parainfluenza virus 5 (PIV5) expressing the influenza A virus hemagglutinin provides immunity in mice to influenza A virus challenge Virology, 362(1): 139-150

22. Fuentes, S., Tran, K.C., Teng, M.N. and He, B. (2007) Function of the respiratory syncytial virus small hydrophobic protein J. Virol. 81(15): 8361-6 (selected as the spotlight for the August 15th issue)

23. Tran, K.C. He, B., and Teng, M.N. (2007) Replacement of the respiratory syncytial virus nonstructural proteins NS1 and NS2 by the V protein of parainfluenza virus 5 Virology 368:72-83

24. Lin, Y., Sun, M., Keim, C. and He, B. (2007) Inhibition of interleukin-6 expression by the V protein of parainfluenza virus 5 Virology 368:262-272

25. Sun, M., Fuentes, S., Timani, K., Murphy, C., Lin, Y., August, A., Teng M.N. and He, B. (2008) Akt plays a critical role in replication of non-segmented, negative-stranded RNA viruses J. Virol. 82:105-14

26. Timani, K., Sun, D., Sun, M., Keim, C., Lin, Y., Schmitt, P., Schmitt, A.P. and He, B. (2008) A single amino acid residue change in the P protein of parainfluenza virus 5 (PIV5) elevates viral RNA replication J. Virol. 82:9123-33

27. Luthra, P., Sun, D., Wolfgang, M., and He, B. (2008) Activation of NF-κB Through an AKT1-dependent Pathway by the L protein of parainfluenza virus 5 (PIV5) J. Virol. 82:10887-95

28. Li, M., Schmitt, P.T., Li, Z., He, B., and Schmitt, A.P. (2009) Requirements for the Production of Mumps Virus-Like Particles J. Virol. 83:7261-72

29. Sun, D., Luthra, P., Li, Z., and He, B. (2009) PLK1 down-regulates parainfluenza virus 5 gene expression PLoS Pathogens 5(7):e1000525

30. Cox, R., Green, T. J., Qiu, S., Kang, J., Tsao, J., Prevelige, P.E., He, B. and Luo, M. (2009) Characterization of a Mumps Virus Nucleocapsid-like Particle. J. Virol. epub

31. Fuentes, S. M., Sun, D., Schmitt, A. P. and He, B. (2010) The role of phosphorylation of P protein in paramyxovirus gene expression Future Micro. (In press)

Contact Information: 0115 HENNING BUILDING, UNIVERSITY PARK, PA 16802. Phone: 814-863-8533; Email: (bxh40@psu.edu)

14. Dr. Ramesh Ramachandran: Associate Professor, Department of Poultry

Science, Pennsylvania State University, University Park, PA 16802, USA

Title 0f the Talk: Novel Adipose Tissue Hormones in Metabolic Diseases and Animal Biotechnology

Abstract:

Adipose tissue is a major endocrine gland in addition to acting as a reservoir of lipids Lower blood levels of adiponectin, a major adipose tissue hormone, is associated with incidence of Type 2 diabetes in humans. Our laboratory is investigating the role of adiponectin in glucose and lipid metabolism using chicken, an animal that is naturally hyperglycemic and exhibit rapid body growth. We have cloned the chicken genes that encode for adiponectin and its receptors, AdipoR1 and AdipoR2. Using custom-generated antibodies, we found adiponectin and its receptors are widely expressed in various organs. Using gel filtration column chromatography, we found adiponectin in chicken plasma and adipose tissue to be predominantly a 669 kDa protein, a heavy molecular weight (HMW) isoform that is correlated with incidence of metabolic disorders in humans. Mass spectrometric analysis of chicken adiponectin revealed a unique collagenous domain containing 22 glycine-X-proline repeats with twice as many lysine residues compared with human adiponectin. We developed an immunoassay to quantify plasma adiponectin in chickens and found that plasma adiponectin levels were significantly lower in 8-week-old compared to 4-week-old male chickens and inversely related to abdominal fat mass. We generated recombinant chicken adiponectin in mammalian cell culture and found that the recombinant adiponectin increased glucose uptake. Collectively, our data provides novel evidence that adiponectin in chicken plasma and tissues is predominantly a HMW multimer, suggesting the presence of unique multimerization possibly due to hyperglycemia and low insulin activity in the chicken. Our research will lead to finding a method to increase the blood levels and activity of HMW adiponectin

isoform that is most beneficial to reduce the incidence of Type 2 diabetes in humans. Furthermore, the recombinant chicken adiponectin could help to improve animal production by increasing nutrient utilization and decreasing visceral fat.

Description of Ramesh Ramachandran:

Dr. Ramesh Ramachandran is an Associate Professor in the Department of Poultry Science at The Pennsylvania State University, University Park, Pennsylvania. He received a BVSc degree from Madras Veterinary College, India, and an M.S in Animal Science and a Ph. D from the University of Maryland, College Park, Maryland. Before joining Penn State in 2002, Dr. Ramachandran conducted research at National Institutes of Health and in US Department of Agriculture both institutions located in Maryland, USA. His area of expertise is studying the role of endocrine system on body growth and reproduction.

Dr. Ramachandran’s research interests and publications can be found at http://www.personal.psu.edu/faculty/r/u/rur11/index.htm.

Biographical Sketch of Dr. Ramesh Ramachandran:

Professional Preparation Madras Veterinary College-- BVSc (DVM)-1980-1986, Veterinary Medicine Univ Maryland (College Park, MD) MS 1992-1994 Poultry Science Univ Maryland (College Park, MD) Ph. D 1995-1997 Animal Science USDA (Beltsville, MD) Post-doc 1998-2000, Endocrinology National Institutes of Health -- Post-doc 2000-2002, Neuroendocrinology Appointments 2008 to present--Associate Professor, Department of Poultry Science, Pennsylvania State University 2002 to 2008-- Assistant Professor, Department of Poultry Science, Pennsylvania State University Publications Hendricks III GL, Hadley JA, Krzysik-Walker SM, Vasilatos-Younken R,

Ramachandran R (2009) Unique profile of chicken adiponectin, a predominantly heavy molecular weight multimer, and relationship to visceral adiposity. Endocrinology, 150:3092-3100.

Ocón-Grove OM, Krzysik-Walker SM, Maddineni S, Hendricks III GL, Ramachandran R (2009) NAMPT (visfatin) in the chicken testis: Influence of sexual maturation on cellular localization, plasma levels, gene and protein expression. Reproduction (In Press).

Bédécarrats GY, McFarlane H, Maddineni, SR, Ramachandran R (2009) Gonadotropin-inhibitory hormone receptor signaling and its impact on reproduction in chickens. General and Comparative Endocrinology, (In Press).

Krzysik-Walker SM, Ocón-Grove OM, Hendricks III GL, Ramachandran R (2008) Is visfatin an adipokine or myokine? Evidence for greater visfatin expression in skeletal muscle than abdominal fat in chickens. Endocrinology, 149(4):1543-1550.

Ocón-Grove OM, Krzysik-Walker SM, Maddineni S, Hendricks III GL, Ramachandran R (2008) Adiponectin and Its receptors are expressed in the chicken testis: Influence of sexual maturation on testicular AdipoR1 and AdipoR2 mRNA abundance. Reproduction, 136:627-638.

Maddineni S, Ocón-Grove OM, Krzysik-Walker SM, Hendricks III GL, Ramachandran R (2008) Gonadotropin-inhibitory hormone (GnIH) receptor expression in the chicken pituitary gland: Potential influence of sexual maturation and ovarian steroids. Journal of Neuroendocrinology, 20:1078-1088.

Maddineni S, Ocón-Grove OM, Krzysik-Walker SM, Hendricks III GL, Ramachandran R (2008) Gonadotropin-inhibitory hormone (GnIH) receptor gene is expressed in the chicken ovary: Potential role of GnIH in follicular maturation. Reproduction, 135(2):267-274.

Krzysik-Walker SM, Ocón-Grove OM, Maddineni SR, Hendricks III GL, and Ramachandran R (2007) Identification of calcitonin expression in the chicken ovary: Influence of gonadal steroids. Biology of Reproduction, 77:626-635.

Maddineni SR, Krzysik-Walker SM, Ocón-Grove OM, Hendricks III GL, and Ramachandran R (2007) Calcitonin is expressed in the chicken pituitary gland: Influence of gonadal steroids and sexual maturation. Cell and Tissue Research, 327:521-528.

Ramachandran R, Ocón-Grove OM, Metzger SL (2007) Molecular cloning and tissue expression of chicken AdipoR1 and AdipoR2 complementary deoxyribonucleic acids. Domestic Animal Endocrinology, 33:19-31.

Ocón-Grove OM, Maddineni SR, Hendricks III GL, Elkin RG, Proudman JA, Ramachandran R (2006) Pituitary progesterone receptor expression and plasma gonadotrophin concentrations in the reproductively dysfunctional mutant restricted ovulator chicken. Domestic Animal Endocrinology, 32:201-215.

Proszkowiec-Weglarz M, Richards MP, Ramachandran R, McMurtry JP (2006) Characterization of the AMP-activated protein kinase pathway in chickens. Comparative Biochemistry and Physiology, Part B 143:92-106.

Maddineni SR, Metzger SL, Ocon OM, Hendricks III GL, Ramachandran R (2005) Adiponectin gene is expressed in multiple tissues in the chicken: food deprivation influences adiponectin messenger ribonucleic acid expression. Endocrinology 146:4250-4256.

Hollon TR, Bek MJ, Lachowicz JE, Ariano MA, Mezey E, Ramachandran R, Wersinger SR, Soares-da-Silva P, Liu ZF, Grinberg A, Drago J, Young WS, 3rd, Westphal H, Jose PA, Sibley DR (2002) Mice lacking D5 dopamine receptors have increased sympathetic tone and are hypertensive. Journal of Neuroscience, 22:10801-10810.

Ramachandran R, Kuenzel WJ, Proudman JA (2001) Increased proliferative activity and programmed cellular death in the turkey hen pituitary gland following interruption of incubation behavior. Biology of Reproduction, 64:611-618.

Weatherly KL, Ramachandran R, Strange H, Waite KL, Storrie B, Proudman JA, Wong EA (2001) The turkey transcription factor Pit-1/GHF-1 can activate the turkey prolactin and growth hormone gene promoters in vitro but is not detectable in lactotrophs in vivo. General and Comparative Endocrinology, 123:244-253.

Ramachandran R, Kuenzel WJ, Buntin JD, Proudman JA (2000) Identification of growth-hormone- and prolactin-containing neurons within the avian brain. Cell and Tissue Research, 299:371-383.

Ramachandran R, Solow R, Proudman JA, Kuenzel WJ (1998) Identification of mammosomatotrophs in the turkey hen pituitary: increased abundance during hyperprolactinemia. Endocrinology, 139:781-786.

Ramachandran R, Proudman JA, Kuenzel WJ (1996) Changes in pituitary somatotroph and lactotroph distribution in laying and incubating turkey hens. General and Comparative Endocrinology, 104:67-75.

Ramachandran R, Proudman JA, Kuenzel WJ (1995) Changes in pituitary somatotrophs and lactotrophs associated with ovarian regression in the turkey hen (Meleagris gallopavo). Comparative Biochemistry Physiology, Part C, 112:327-334.

(d) Synergistic Activities

• Member of the USDA NRI competitive Grants Program Review Panel, Reproductive Biology Section, 2006, 2007, and 2008.

• Associate Editor, Poultry Science journal (2006 to present) • Ad hoc reviewer for national and international funding agencies: National

Science Foundation, USDA-BARD grant program, Israel Science Foundation, and Smithsonian Institution.

Contact Information: Ramesh Ramachandran, Associate Professor of Endocrinology, Department of Poultry Science, 211 Henning, Pennsylvania State University. University park, PA 16802. Phone: 814-865-5202; Email: (rameshr@psu.edu).

15. Matthew Ferrari: Center for Infectious Disease Dynamics, Department

of Biology, 208 Mueller Lab, The Pennsylvania State University

University Park, PA 16802

Title of the Talk: Using computational models to inform vaccine policy in West Africa. ABSTRACT:

Public health and vaccine policy for measles in western Africa is faced with both logistical and dynamical challenges. Practically, there are powerful constraints on distribution of vaccine and access to care that make the application of uniform strategies a challenge. Dynamically, strong seasonal forcing and high birth rates lead to locally unstable outbreak dynamics. Consequently, large-scale regional persistence of measles depends on spatial coupling among communities across huge areas

and strong demographic and seasonal gradients. Understanding these dynamics and their implications for regional heterogeneities in population level immunity is key

to developing effective public health policy. We use computational statistics to analyze long-term surveillance data for measles in Niger to identify the key factors that contribute to the persistence of measles in the region. We then use simulation models to explore novel vaccination strategies for the region and explore the consequences of new advances in vaccine technologies. The unique combination of high birth rates and locally unstable epidemic dynamics suggest that vaccine strategies based on a conventional understanding of measles epidemiology will be inefficient compared to the experience in Europe and North America. Consequently, efficient vaccine strategies should be locally reactive to temporal and spatial variation in the population level distribution of immunity. This further suggests that investment in logistical infrastructure to facilitate efficient distribution of vaccine and reactive strategies is a key challenge in this region.

Biographical Sketch of Dr. Matthew Ferrari:

EDUCATION:

2002-2006 Ph.D. Ecology, the Pennsylvania State University, University Park, PA. Advisor: Dr. Ottar Bjørnstad

1999-2002 M.Sc. Statistics, Montana State University, Bozeman, MT Advisor: Dr. Steve Cherry

1996-1999 M.Sc. Fish and Wildlife Management Program, Montana State University, Bozeman, MT. Advisor: Dr. Robert Garrott

1992-1996 B.A. Biology, Magna Cum Laude Colby College, Waterville, ME

PROFESSIONAL POSITIONS:

2007-current . Research Associate, Center for Infectious Disease Dynamics,

Department of Biology, the Pennsylvania State University

2006-2007 Post-doctoral researcher, Center for Infectious Disease Dynamics,

Department of Biology, the Pennsylvania State University

FELLOWSHIPS AND AWARDS:

2009-2012- USDA-BRAG: Development and Validation of a Model for the Spread and Management of an Escaped Virus Resistant Transgene in Wild Populations of Cucurbita. (PI Andrew Stephenson, Co-PI Ferrari), $397,000.

2000-2012- Evaluating candidate vaccine technologies using computational methods. Bill and Melinda Gates Foundation. (Sub-contract to Penn State, Co-PI). $622,912.

2009-2010-WHO contract to develop statistical methodology for estimating global burden of measles mortality. PI: Ferrari, $34,900.

2010-2011- NIH contract # HHSN275200900401P. Research contract under Research and Policy in Infectious Disease Dynamics (RAPIDD) program. PI: Ferrari, $25,000.

2007 -NIH Grant #GM83606. Novel statistical models for synthesizing social networks and epidemic dynamics. $1,400,000

2004-2006 Penn State Academic Computing Fellowship $75,000

2002-2004 Braddock Award $12,000

2002-2003 Penn State University Graduate Fellowship $22,250

2004 Brian Horton Award, Penn State IGDP in Ecology $1,500

PUBLICATIONS:

Stack, J.C., D. Welch, M.J. Ferrari, B. Shapiro, B.T. Grenfell. Protocols for samping viral sequences to study epidemic dynamics. (in review).

Ferrari, M.J. A. Djibo, R.F. Grais, N. Bharti, B.T. Grenfell, O.N. Bjornstad. Rural urban gradient in seasonal forcing of measles transmission in Niger (in review).

Bharti N, R.F. Grais, M.J. Ferrari, O.N. Bjørnstad, A. Tatem, C. McCabe, A. Djibo, B.T. Grenfell. (in press) Measles hotspots and epidemiological connectivity in Niger. Epidemiology and Infection.

Sasu, M, M.J. Ferrari, D. Du, J.A. Winsor, A.G. Stephenson. 2009. Indirect costs of a non-target pathogen mitigate the direct benefits of a virus resistant transgene in wild Cucurbita. Proceedings of the National Academy of Science106: 19067-19071 -- doi: 10.1073/pnas.0905106106

Ferrari, M.J., A. Djibo, R.F. Grais, B.T. Grefell, O.N. Bjørnstad. (in press) Episodic outbreaks bias estimates of age-specific force of infection: a corrected method using measles in Niamey, Niger as an example. Epidemiology and Infection.

Perkins, S.E., M.J. Ferrari, P.J. Hudson. 2008. The effects of social structure and sex-biased transmission on macroparasite infection. Parasitology doi: 10.1017/S0031182008000449.

Ferrari, M.J., R.F. Grais, N. Bharti, A.J.K. Conlan, O.N. Bjørrnstad, L.J. Wolfson, P.J. Guerin, A. Djibo, B.T. Grenfell. 2008. The dynamics of measles in sub-Saharan Africa. Nature 451: 679-685.

Grais, R.F., A.J.K. Conlan, M.J. Ferrari, O.N. Bjørnstad, B.T. Grenfell, A. Djibo, P. Strebel. 2008. Time is of the Essence: Exploring a Measles Outbreak Response Vaccination in Niamey, Niger. Journal of the Royal Society Interface 5: 67-74. DOI 10.1098/rsif.2007.1038.

Finkelman, B.S., C. Viboud, K. Koelle, M.J. Ferrari, N. Bharti, G.T. Grenfell. 2007. Global Patterns in Seasonal Activity of Influenza A/H3N2, A/H1N1, and B from 1997 to 2005: Viral Coexistence and Latitudinal Gradients. PLoS ONE 2(12): e1296. doi:10.1371/journal.pone.0001296.

Graham, A. I.M. Cattadori, J. O. Lloyd-Smith, M.J. Ferrari, O.N. Bjørnstad. 2007. Transmission consequences of co-infection: cytokines writ large? Trends in Parasitology. 23: 284-291.

Ferrari, M.J., D. Du, J.A. Winsor, A.G. Stephenson. 2007. Inbreeding depression of plant quality reduces incidence of an insect-borne pathogen in a wild gourd. International Journal of Plant Sciences. 168: 603-610.

Ferrari, M.J., A.G. Stephenson, M.C. Mescher, C.M. De Moraes. 2006. Inbreeding effects on blossom volatiles in Cucurbita pepo ssp. texana. American Journal of Botany 93: 1768-1774.

Ferrari, M.J., O.N. Bjørnstad, J.L. Partain, J. Antonovics. 2006. A gravity model for the spread of a pollinator-borne plant pathogen. The American Naturalist. 168: 294-303. Ferrari, M.J. S. Bansal, L.A. Meyers, O.N. Bjørnstad. 2006. Network frailty and the geometry of herd immunity. Proceedings of the Royal Society B: Biological

Sciences 273: 2743-2748.

Grais, R.F., M.J. Ferrari, C. Dubray O.N. Bjørnstad, B.T. Grenfell, A. Djibo, F. Fermont, P.J. Guerin. 2006. Estimating the reproductive ratio for a measles epidemic in Niamey, Niger: lessons for intervention. Transactions of the Royal Society of Tropical Medicine and Hygiene. 100(9): 867-873.

Ferrari, M.J., Bjørnstad,O.N. and Dobson, A.P. 2005. Estimation and inference for R0 of an infectious disease using a removal method. Mathematical Boisciences. 198(1): 14-26.

Ferrari, M.J. and R.A. Garrott. 2002. Bison and elk: brucellosis seroprevalence on a shared winter range. Journal of Wildlife Management 66(4): 1246-1254.

THESiS:

Ferrari, M.J. 2006. Mixing models and the geometry of epidemics. Ph.D. Dissertation. The Pennsylvania State University, University Park, PA.

Ferrari, M.J. 1999. An assessment of the risk of inter-specific transmission of Brucella abortus from bison to elk on the Madison-Firehole winter range. Master's Thesis. Montana State University, Bozeman, MT.

PROFESSIONAL DEVELOPMENT:

2007- NCEAS working group on Unifying approaches to statistical inference in ecology.

2005- Robustness of networks to evolving agents. Working Group. Santa Fe Institute. Santa Fe. NM.

2004- Short Course, Uncertainty and variability in ecological inference, forecasting, and decision-making. Duke University

Contact Information:208 Mueller Laboratory, University Park, PA 16802. Phone: 814-865-6080; Email: (mjf283@psu.edu) or (mferrari@psu.edu)

Please note that Sagarika is listed under the following session: Anticancer Drugs Issues and Challenges – Session Biopharmaceuticals VI 16. Sagarika Kanjilal, Departments of Pharmacology, College of Medicine, Pennsylvania State University at Hershey and Department of Veterinary and Biomedical Sciences, Penn State University, University Park, PA 16802. Title of the Talk: Fibrosarcoma: Molecular Profiling of Disease Progression through Comparative Studies Abstract:

The treatment of cancers is complicated both by the development of resistance and systemic toxicity to existing drugs, as well as the paucity of approved effective targeted therapeutics. The problem is particularly acute for certain cancers such as adult-onset fibrosarcomas, which are highly invasive tumors of mesenchymal origin. These tumors are notoriously refractory to treatment with existing therapeutic agents (such as doxorubicin), and are associated with substantial morbidity and mortality. A better understanding of the molecular mechanisms underlying tumor development and progression is necessary in order to develop new and rational targets for therapeutic intervention of this disease. In order to overcome the deficit of appropriate and well-characterized tissue samples from human fibrosrcoma patients, we adopted a comparative approach and assembled a tissue model of fibrosarcoma progression in domestic cats (a mammalian species whose genome bears a remarkable degree of syntenic conservation with that of humans). Tissue samples were collected from primary tumors, post-surgical local recurrences, and distant metastases from individual feline patients, and characterized using various in vitro and in vivo assays of tumor cell aggressiveness in order to validate the tissue-model as a clinically relevant platform for our studies on malignant progression. Matched feline fibrosarcoma samples (along with appropriate controls) were subjected to genomics and proteomics based analyses and the results verified in both model animal as well as

human tissues. The results of these investigations provide strong leads for cytokines and cellular signal transduction molecules responsible for diverse aspects of tumorigenesis including inflammation, cellular proliferation, hypoxia, angiogenesis, migration, invasion, drug resistance, and metastasis. Ongoing analysis of upstream and downstream aspects of the specific signal molecules and pathways so identified will enable the development of individualized intervention targets for fibrosarcoma. Biographical Sketch of Sagarika Kanjilal: NAME: Sagarika Kanjilal POSITION TITLE: Associate Professor

EDUCATION/TRAINING

INSTITUTION AND LOCATION DEGREE

(if applicable)

YEAR(s) FIELD OF STUDY

St. Xavier’s College, Calcutta, India Bsc 1982 Chemistry (Honors) Univ. of Calcutta, Calcutta, India MSc 1984 Biochemistry

Univ. of Calcutta, Calcutta, India PhD 1988 Biochemistry

Pennsylvania State University, Univ. Park, PA

PhD 1992 Molecular & Cell Biology

Positions 2009-present: Graduate Faculty, Integrative Biosciences Programs in Molecular Medicine, Bioinformatics & Genomics, Molecular Toxicology and Carcinogenesis, and Pathobiology, Penn State, Univ. Park, PA.

2008-present: Assoc. Prof., Depts. of Pharmacology & Vet. & Biomed. Sciences, Penn State, Univ. Park, PA.

2004-2007: Associate Professor, Dept. of Medicine, Medical School, Univ. of Minnesota, Twin Cities, MN.

2001-2007: Steering Committee, Biomedical Genomics Center, Univ. of Minnesota, MN.

1996-2004: Assistant Prof., Depts. of Dermatology & Vet. Pathobiology, Univ. of Minnesota, Twin Cities, MN.

1992-96: Res. Assoc., Immunology, Epidemiology,& Dermatology, M. D. Anderson Cancer Center, Houston, TX.

1991-92: Postdoctoral Research Associate, Dept. of Pathology, Baylor College of Medicine, Houston, TX.

Honors 2002: Certificate of Appreciation for extraordinary service in animal health, Morris Animal Foundation

1996: Women in Cancer Research Brigid G. Leventhal Award sponsored by Hoechst Marion Roussel

1993-94: R. E. Smith Research Foundation Fellowship

1990-91: Penn State Univ. Graduate School Award

1991: Procter and Gamble Travel Award

1989-90: Penn State Univ. Graduate School Award

1989: Brookhaven National Laboratory Symposium Fellowship

1984: Presidents Medal for excellence in studies towards a Masters degree in Biochemistry

Selected Publications Y. Sohni, S. Kanjilal, and V. Kapur. 2008. Performance evaluation of five commercial real-time PCR reagent systems using TaqMan assays for B. anthracis detection. Clinical Biochemistry 41: 640-644.

Y. Sohni, S. Kanjilal, and V. Kapur. 2008. Cloning and development of synthetic internal amplification control for B. anthracis real-time polymerase chain reaction assays. Diagn Microbiol Infect Dis. 61: 471-5.

N. Banerji, V. Kapur, and S. Kanjilal. 2007. Association of Germ-line Polymorphisms in the Feline p53 Gene with Genetic Predisposition to Vaccine-Associated Feline Sarcoma. J. Hered. 98:421-7.

N. Banerji and S. Kanjilal. 2006. Somatic alterations in the p53 gene in vaccine-associated feline sarcoma. Am. J. Vet. Res. 67:1766-72.

L. Li, S. Munir, J.P. Bannantine, S. Sreevatsan, S. Kanjilal, and V. Kapur. 2006. Rapid expression of Mycobacterium avium subsp. paratuberculosis recombinant proteins for antigen discovery. Clin Vaccine Immunol. 14:102-5.

L. Li, J.P. Bannantine, Q. Zhang, A. Amonsin, B.J. May, D. Alt, N. Banerji, S. Kanjilal, and V. Kapur. 2005. The Complete Genome Sequence of Mycobacterium avium subsp. paratuberculosis. Proc. Natl. Acad. Sci, USA. 102:12344-9.

N. Banerji, X. Li, J.S. Klausner, V. Kapur, and S. Kanjilal. 2002. In vitro chemosensitivity of vaccine-associated feline sarcoma cell lines to vincristine and paclitaxel. Am. J. Vet. Res. 63:728-32.

L. Williams, N. Banerji, J.S. Klausner, V. Kapur, and S. Kanjilal. 2001. Establishment of two vaccine-associated feline sarcoma cell lines and in vitro testing of chemosensitivity to doxorubicin and mitoxantrone. Am. J. Vet. Res. 62:1354-1357.

S. Kanjilal, B.R. Nelson, N. Banerji, and V. Kapur. 1999. Rapid Detection of p53 Mutations in Basal Cell Carcinoma Margins. J. Invest. Dermatol. 112:558.

A. Chatterjee, S. Kanjilal, A.K. Bhattacharyya. 1999. Purification of human seminal acrosin inhibitor and its kinetics. J. Biol. Sci, 24 : 323-328.

L. Williams, J. Zinggeler, J. Klausner, and S. Kanjilal. 1998. Field Cancerization. Vet. Can. Soc. News 4:8-9.

S. Kanjilal and M. Duvic. 1997. Cutaneous carcinogenesis: Molecular progression model of cancer development. In Cutaneous oncology: Pathophysiology, Diagnosis, & Treatment. S.J. MIller, M.E. Maloney (eds.), Blackwell Sc. Co. Pp 19-29.

H. N. Ananthaswamy and S. Kanjilal. 1996. Oncogenes and tumor suppressor genes in photocarcinogenesis. Photochem. Photobiol. 63: 428-430.

S. Kanjilal, S.S. Strom, G.S. Clayman, R.S. Weber, A.K. El-Naggar, V. Kapur, K.K. Cummings, L.A. Roth, M.R. Spitz, M.L. Kripke, and H.N. Ananthaswamy. 1995. p53 mutations in nonmelanoma skin cancer of the head and neck: molecular evidence for field cancerization. Cancer Res. 55: 3604-3609.

S. Kanjilal and H.N. Ananthaswamy. 1995. Molecular biology of skin cancer. In Basal and squamous cell skin cancers of the head & neck. R. S. Weber, M. Miller, H. Goepfert (eds), Lea & Febiger, PA. pp 25-36.

S. Kanjilal and H.N. Ananthaswamy. 1995. Epidermal malignant neoplasms: photocarcinogenesis. In Cutaneous medicine & surgery: an integrated program in dermatology. K.A. Arndt, P.E. LeBoit, J.K. Robinson, B.U. Wintroub (eds), W.B. Saunders, P/A. pp 1363-1377.

S. Kanjilal and H.N. Ananthaswamy. 1995. The role of oncogenes and tumor suppressor genes in UV carcinogenesis. In Skin cancer: mechanisms and human relevance. H. Mukhtar (ed), CRC Press, FL. pp 305-316.

V. Kapur, S. Kanjilal, M.R. Hamrick, T.S. Wittam, S. Sawyer, and J.M. Musser. 1995. Molecular population genetic analysis of streptokinase gene of Streptococcus pyogenes:mosaic alleles generated by recombination. Mol. Microbiol. 16:509-519.

S. Kanjilal, W.E. Pierceall, K.K. Cummings, M.L. Kripke, and H.N. Ananthaswamy. 1993. High frequency of p53 mutations in ultraviolet radiation-induced murine skin tumors: Evidence for strand bias and tumor heterogeneity. Cancer Res. 53: 2961-2964.

S. Kanjilal, W.E. Pierceall, and H.N. Ananthaswamy. 1993. Ultraviolet radiation in the pathogenesis of skin cancers: Involvement of ras and p53 genes. Cancer Bull. 45: 205-211.

J.M. Musser, V. Kapur, S. Kanjilal, U. Shah, N.L. Barg, K.H. Johnston, P.M. Schlievert, et. al. 1993. Geographic and temporal distribution and molecular characterization of two highly pathogenic clones of Streptococcus pyogenes expressing allelic variants of pyrogenic exotoxin A (Scarlet fever toxin). J. Infect. Dis. 167: 337-346.

J.M. Musser, K. Nelson, R.K. Selander, D. Gerlach, J.C. Huang, V. Kapur, and S. Kanjilal. 1993. Temporal variation in bacterial disease frequency: Molecular population genetic analysis of Scarlet fever epidemics in Ottawa, Canada, and eastern Germany. J. Infect. Dis. 167: 759-762.

A.K. Bhattacharyya, S. Kanjilal, H. van der Ven, K. Diedrich. 1993. Some biochemical parameters controlling sperm-egg interaction. Medical Bulletin: Institute of Reproductive Medicine. 9: 20-32.