©2014 waters corporation 1 enhanced ms with the turn of key
TRANSCRIPT
©2014 Waters Corporation 1
ENHANCED MS WITH THE TURN OF KEY
©2014 Waters Corporation 2
Introducing ionKey/MS
What does ionKey/MS™ deliver?
• 2-40x sensitivity vs. your current MS
•UPLC® performance and reliability
•Standardization and consistency
• Flexibility
©2014 Waters Corporation 3
In Search of a Step Change in
Sensitivity?
©2014 Waters Corporation 4
Maximizing signal
Maximizing robustness
Xevo® TQ-SA Step Change in Sensitivity
©2014 Waters Corporation 5
Time1.80 1.90 2.00 2.10 2.20 2.30
%
0
Time1.80 1.90 2.00 2.10 2.20 2.30
%
1
Xevo TQ
Xevo TQ-SEnhanced sensitivity
>25X increase in peak area>5X increase in signal:noise
Rela
tive ion a
bundance
Reserpine (50fg) UPLC/MRM
Xevo TQ-SA Step Change in Sensitivity
©2014 Waters Corporation 6
Where Next to Look for
Sensitivity?
©2014 Waters Corporation 7
Where to Next?
75µm ID 150µm ID 300µm ID +
Very Sensitive
Flow rates too low for routine
use
Very Fast
Marginal sensitivity increase
0
Fast and Sensitive
Useable routinely
©2014 Waters Corporation 8
THE FUTUREOF MS
UPLCPERFORMANCE
YOU KNOW
MORE SENSITIVITY
PLUG ANDPLAY
ROBUSTREPRODUCIBLE
RELIABLE
©2014 Waters Corporation 9
THE FUTUREOF MS
UPLCPERFORMANCE
YOU KNOW
MORE SENSITIVITY
PLUG ANDPLAY
ROBUSTREPRODUCIBLE
RELIABLE
©2014 Waters Corporation 10
More Sensitivity
ionKey/MS
20X Increase
Standard 2.1mmcolumn
©2014 Waters Corporation 11
More Sensitivity
ionKey/MS Standard Flow LC-MS
ionKey/MS
2 Fold suppression in signal due
to Matrix Effects
2.1mm
24 Fold suppression in signal due
to Matrix Effects
©2014 Waters Corporation 12
THE FUTUREOF MS
UPLCPERFORMANCE
YOU KNOW
MORE SENSITIVITY
PLUG ANDPLAY
ROBUSTREPRODUCIBLE
RELIABLE
©2014 Waters Corporation 13
UPLC Performance You Know
©2014 Waters Corporation 14
THE FUTUREOF MS
UPLCPERFORMANCE
YOU KNOW
MORE SENSITIVITY
PLUG ANDPLAY
ROBUSTREPRODUCIBLE
RELIABLE
©2014 Waters Corporation 15
Sensitivity
ReproducibilityRobustnessUser Expertise RequirementApplicability to Routine
Capillary scale UPLC can be challenging
©2014 Waters Corporation 16
ionKey/MS is Plug and Play
ENHANCED MS WITH THE TURN OF KEY
©2014 Waters Corporation 17
THE FUTUREOF MS
UPLCPERFORMANCE
YOU KNOW
MORE SENSITIVITY
PLUG ANDPLAY
ROBUSTREPRODUCIBLE
RELIABLE
©2014 Waters Corporation 18
Robust, Reliable Reproducible
Retention Time Reproducibility
15 QCs over 500 crashed plasma injections
Reproducibility of the same compound over:
1.64% RSD
5 Systems18 iKeys
5 Users
©2014 Waters Corporation 19
Validated Assay
Teriparadite (Forteo®) Avg MWT 4117.7
Teriparatide Concentration
(pg/mL)Teriparatide/IS Ratio Response
Calculated Teriparatide
Concentration (pg/mL)
Mean Accuracy
10.00 0.07 10.56 105.6320.00 0.14 20.53 102.6340.00 0.29 38.99 97.5860.00 0.43 57.58 95.97100.00 0.73 97.00 97.00300.00 2.17 286.39 95.50600.00 4.75 626.81 104.451000.00 8.05 1061.49 106.153000.00 22.31 2937.14 97.95
Teriparatide QC
Concentration (pg/mL)
Mean (N=5) Calculated
Concentration (pg/mL) SD %CV
Mean Accuracy
25 25.8887 1.32 5.09 103.6
50 51.4236 1.91 3.72 102.8
80 83.8803 2.15 2.56 104.9
200 202.3569 6.49 3.20 101.2
500 511.1018 15.23 2.98 102.2
©2014 Waters Corporation 20
THE FUTUREOF MS
UPLCPERFORMANCE
YOU KNOW
MORE SENSITIVITY
PLUG ANDPLAY
ROBUSTREPRODUCIBLE
RELIABLE
©2014 Waters Corporation 21
The Future of MS
Already over 25 publications and presentations in leading scientific journals and conferences such as:
Journal of Chromatography, Bioanalysis Journal, Journal of Proteomics, ASMS, HPLC, EBF
CPSA, and more.
©2014 Waters Corporation 22