LC/MS/MS

VARIAN, INC.

325-MS

www.varianinc.com

TRIPLE QUADRUPOLE MASS SPECTROMETER

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Small Molecules – Big ChallengesVARIAN, INC.

Your samples are precious and turnaround times are critical to the

success of your organization. A rugged instrument that provides

day-in, day-out performance is a cornerstone to your workflow.

The Varian 325-MS is a robust, reliable option for many applications,

including: environmental, food safety, clinical research and forensics.

Designed to handle difficult small molecule analyses, it offers a choice

of ionization modes and scanning techniques to ensure you obtain the

information you are looking for.

The Varian 325-MS innovative technologies facilitate increased

productivity in a high throughput environment.

It features Varian’s patent pending vESI™ ion source, a vortex API, and

patent pending Gold Guard™ ion optics that jointly enhance sensitivity,

ruggedness and throughput. The higher sensitivity means you can

analyze more compounds in a single run. The more rugged design

means less downtime for cleaning the source. That adds up to an

extremely productive solution.

The standard configuration contains: 325-MS with vESI interface, 212-LC pumps

and choice of autosampler (shown here with the 460-LC autosampler).

The Varian 325-MS Provides:• Accurate and reproducible results in difficult matrices

• Highest sensitivity among comparable triple quadrupole instruments

• Easy-to-use vESI interface

• Increased number of target analytes possible in multi-residue methods

• Reduced need for front-end maintenance

For more than 40 years, Varian has provided high quality instrumentation

to the scientific community. We are committed to providing high

performance and reliable analytical instruments. Our main objectives are to

satisfy your current needs and future workflow requirements.

Tap into Varian’s expertise throughout the lifetime of your instrument.

Our network of expert engineers and application specialists are ready

to assist you from system installation to method development, all

geared towards improving your productivity and lowering your overall

cost of ownership.

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Redefining Benchtop LC/MS/MS Performance

Features of the 325-MSvESI™ Ion Source

The patent pending vESI ion source employs a novel vortex

mechanism for improved heat transfer and greater desolvation

efficiency of sample droplets. More ions and less matrix are

transferred into the mass spectrometer, increasing sensitivity and

reducing contamination.

Gold Guard™ Ion Optics

The most robust ion optics system currently available, Gold Guard

is engineered to withstand repeated analyses in complex matrices

without a loss of signal, resulting in less maintenance and greater

uptime.

Skimmer Cone and Q0 Assemblies

The redesigned skimmer cone and ion guide increase ion

sampling and transmission efficiency, resulting in improved gas

conductance, lower ion guide pressure and increased signal.

180° Collision Cell

A 180° lens-less assembly with square geometry reduces noding and

increases ion transmission for enhanced sensitivity. A long collision cell

path length provides for a wide CID energy range. The geometry of the

assembly removes the line-of-sight path to the detector for neutrals,

photons and meta-stable ions, reducing noise.

On-Axis Detector

An on-axis detector (with respect to Q3) at ±5 kV avoids poor

sensitivity for negative ions and inherent noise in conversion

dynode systems.

Dual-Stage Turbomolecular Pump

The single pump design efficiently evacuates the source and

analyzer regions for simplicity and lower cost. The Varian-designed

and manufactured pump allows for high foreline tolerances and

sustainable throughput with lower operating temperatures.

180° Collision CellvESI Ion Source

On-Axis Detector

Skimmer Cone and Q0 Assemblies

Gold Guard

Ion Optics

Dual-Stage Turbomolecular Pump

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vESI™ Ion Source: Increased SensitivityVARIAN, INC.

Novel API Design Features a Highly Efficient Vortex Electrospray Ionization (vESI) Interface The vESI source for the 325-MS system maximizes ionization efficiency

by application of a heated auxiliary gas rotating with respect to the

nebulizing gas flow; it is used to accelerate evaporation of the liquid

droplets. The droplet stream, nebulizing gas and circular gas form a

vortex, which enhances heat exchange between liquid droplets and

hot gas. The increased ion yield in the gas phase equates to greater

sensitivity (10x) and reduced noise.

The vESI source provides greater efficiency in ionization over a wide

range of liquid flow rates and mobile phase combinations routinely

used in food safety, forensic, toxicology, environmental and drug

discovery applications.

Photos of light scattered by liquid droplets within the vESI ion source with the vortex gas off (left) and on (right). With the vortex gas on, less light is scattered due to faster evaporation, demonstrating greater desolvation efficiency. This leads to increased sensitivity.

Caffeine and carbamazepine were separated on-column using a gradient elution. Representative chromatograms of caffeine and carbamazepine show improvement with the vESI source, when compared to standard ESI operation. Conditions used for both methods were fully optimized.

The vESI source delivers improved heat transfer and desolvation of sample drop-lets, over a wide range of mobile phase flow rates and compositions. More ions are transferred into the mass spectrometer, increasing sensitivity and reducing noise.

vESI gas off vESI gas on

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peak

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injection number

100% Water 10% Methanol 20% Methanol 30% Methanol 40% Methanol 50% Methanol 60% Methanol 70% Methanol 80% Methanol 90% Methanol

Gold Guard™ Ion Optics: Ultimate Resistance Against Contamination

Effectively Limit Front-End Contamination The patent pending Gold Guard ion optics provide enhanced

contamination resistance when analyzing compounds, particularly those

derived from complex matrices. After repeated injections of analytes

suspended in a complex matrix, the 325-MS requires little-to-no

cleaning to maintain a high level of reproducibility and accuracy.

The resistance against contamination provided by the Gold Guard ion

optics means that downtime for cleaning and servicing the API interface

and front-end ion optics is greatly reduced.

The complex, dirty matrix that accumulates on the spray shield (left) is easily removed. Cleaning the Gold Guard with a moist wipe restores the chemically resistant shield to its original condition (right).1,050 total flow injections (105 injections at each of 10 mobile phase conditions)

of chlorpheniramine in a QuEChERS carrot extract, RSD=5.31%. The data were collected with a gradient method that performed 10 flow-injection events (evenly spaced) that progressed from 100% water to 100% methanol.

As a test of system robustness, a carrot mixture was extracted using

the QuEChERS approach, without any cleanup. Flow injections of

chlorpheniramine were made directly into the vESI™ source with no

observable changes in signal intensity or precision of the measurements.

After the injections were complete, the spray shield was noticeably

fouled but the contamination did not affect instrument performance,

due to the protection provided by the Gold Guard ion optics. The Gold Guard and patented off-axis angle of the capillary combine to reduce noise and matrix contamination.

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Ease of UseVARIAN, INC.

Increase Productivity The Varian 325-MS system provides cost-effective, rapid and confident

sample analysis for a variety of analytical applications. The easy-

to-use vESI ion source requires practically no user intervention or

optimization, facilitating data acquisition over a wide range of flow

rates with no flow split. Furthermore, the vESI ion source delivers

unprecedented robustness for demanding analytical applications, while

maintaining a high level of reproducibility and accuracy.

One of the major problems faced by ESI is rapidly delivering thermal

energy into liquid droplets in order to quickly desolvate the solvent-

solute clusters. The problem is exacerbated with higher liquid flows

and/or higher fractions of hydrophilic solvents. The graph below

represents flow injections of rhodamine 6G, ephedrine, chlorpheniramine,

phenylpropanolamine and creatinine at a linear velocity of 400 µL/min

in 75% H2O: 25% acetonitrile, while adjusting the vESI gas temperature.

Even at a flow rate of 400 µL/min in predominantly aqueous solvent,

the analyte signal intensity continues to maintain a steady response,

representing a broad optimization range.Methods can be developed to allow one set of vESI source conditions

to be used throughout a method. The MRM chromatograms above

depict the detection of 11 compounds in a 15-minute run. The

method contains over 25 MRM transitions containing both positive

and negative ions, but only one set of vESI conditions for this diverse

compound set. For very labile compounds or unusually large changes in

mobile phase composition, the temperature can be adjusted if needed.

The 325-MS is equipped with SelecTemp™ control, which affords

changing the drying gas and vESI gas temperatures throughout an

analytical run.

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Rhodamine 6G

Vortex temperature, degrees C

EphedrineChlorpheniramine

Phenylpropanolamine

Creatinine

Permethrin

Acephate

Carbendazim

Thiabenzadol

Carbaryl

Oxamyl

Imazalil

Methidathion

Pirimiphos-methyl

Chlorpyrifos-methyl

Chlorpyrifos

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Setup, Acquire, Analyze and Report

The Complete Solution… The vast majority of mass spectrometry-based methods require various

forms of sample preparation and chromatographic techniques prior to

mass analysis. Varian can offer this exact blend of sample preparation,

chromatography systems, HPLC columns, software and support

required to meet the rigorous needs of your application.

MS Workstation SoftwareInstrument control and data processing are simple with Varian MS

Workstation software.

• Complete single-point control of pumps, autosamplers

and other accessories

• Fully automated tuning and calibration

• Superior data transfer rate with dual processors

• Simple navigation for reviewing and processing result

• Full featured network compatibility for file management, printing

and remote access

• Extensive reporting for complete analyses

• Automated MS/MS optimization

HPLC Pumps212-LC pumps deliver pulse-free flow at low flow rates, ideal for

analyzing limited amounts of sample on a small (2 mm) diameter

column. The low flow rates reduce maintenance on the spectrometer

and increase the sensitivity for analyses of small samples.

AutosamplersHTS PAL™ is a low loss, high speed, high capacity, robust autosampler

offering outstanding flexibility and productivity.

The 460-LC handles well plates and sample vials, open or sealed.

Internal standard addition, sample dilution or derivatization steps

are easily programmed to reduce sample preparation time and errors

associated with manual procedures.

HPLC ColumnsPursuit™ columns are available in a variety of phases and deliver

faster separations without sacrificing resolution. Pursuit Diphenyl

offers unique pi-pi selectivity for aromatic and double bond containing

analytes and improved LC/MS sensitivity. Pursuit PFP delivers optimal

separation of polar analytes under high aqueous conditions.

Polaris™ columns provide unique bonded phases that maximize

polar retention and selectivity, while virtually eliminating silanol

activity. Polar-modified chemistries of this class are very effective for

separations that are difficult on conventional alkyl-bonded phases, and

they are stable in all commonly used HPLC and LC/MS eluents from pH

1.5 to 10.0.

Varian 325-MS LC/MS with SRM transitions offers high quality quantification with more than 10 data points per peak and an analysis of 48 pesticides in less than 11 minutes.

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TRIPLE QUADRUPOLE MASS SPECTROMETER

VARIAN, INC.

325-MS

SI-2227 10/09 Printed in U.S.A.

Varian Care Program Our goal is to help you increase your productivity, maximize your uptime and

achieve the highest return possible on your investment. Our experienced and

highly-qualified support organization is strategically located throughout the world

to ensure rapid response.

Be confident knowing that your instrument will deliver maximum performance,

your users are fully trained and expert technicians will respond quickly to your

support needs — because Varian cares.

Varian offers robust instrumentation, application-based consumables, and customer-focused services, backed by our global team of product and applications experts, ready to help you solve your analytical challenges. Whether you’re monitoring impurities in drinking water, designing new therapeutic drugs, or developing cleaner fuels, our solutions deliver the sensitivity, flexibility and productivity your laboratory requires.

vESI, Gold Guard, SelecTemp, Pursuit, Polaris, Varian, the Varian logo and the Varian Care logo are trademarks or registered trademarks of Varian, Inc. in the U.S. and other countries. HTS PAL is a trademark of CTC Analytics AG. © 2009 Varian, Inc.

Shop OnlineVarian offers the added convenience of purchasing products online. The products

you need are just a few clicks away at www.varianinc.com

We are committed to meeting your needs with a full range of consumable

products and supplies. Call today to request your free copy of our comprehensive

analytical supplies catalog or download it from our web site.

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Varian, Inc. www.varianinc.com North America: 800.926.3000, 925.939.2400Europe The Netherlands: 31.118.67.1000Asia Pacific Australia: 613.9560.7133Latin America Brazil: 55.11.3238.0400

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