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Agilent Bio-Monolith Protein A and Protein G Affinity Columns
WIDER SELECTIVITY FOR mAb QUANTITATION AND ANALYSIS
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AGILENT BIO-MONOLITH PROTEIN A AND PROTEIN G AFFINITY COLUMNS
• Fast separations decrease method development time and costs and provide a larger window of opportunity for harvest.
• High binding capacity allows titer determination across relevant concentration ranges and enables purification for further characterization.
Wide concentration range
Figure 1. Linearity of IgG1 quantitation achieved using the Agilent Bio-Monolith Protein A column over the typical mAb titer range encountered with CHO cells.
DETERMINE MAB TITERS QUICKLY AND ACCURATELY
The ability to measure monoclonal antibody (mAb) titers and to purify small amounts of product candidate for further characterization is essential for the development of an economic production process.
Agilent Bio-Monolith Protein A and Protein G columns deliver consistently excellent performance under the conditions encountered during method development, optimization, and production. These columns are ideal for titer determination during process development, cell clone selection, and optimization of the fermentation conditions, and in production to determine the optimum time to harvest – helping to ensure correct cell line choice and identification of harvest times to increase process yield and economics.
• Now with the new Protein G Monolith column there is a wider range of selectivity for IgG subclasses.
• Long column life and minimal clogging, even with complex samples, means less frequent column cleaning and replacement.
An unusual shape? Perhaps. But Agilent Bio-Monolith Protein A and Protein G columns deliver the high capacity and performance you need for reproducible affinity chromatography.
R2 = 0.9999
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AGILENT BIO-MONOLITH PROTEIN A AND PROTEIN G AFFINITY COLUMNS
Proven specificity
Figure 2. Rapid and specific capture of IgG1 from cell culture supernatant using the Bio-Monolith Protein A column. (A) Escherichia coli cell lysate supernatant (2 ug of IgG1 and 38 ug of cell lysate proteins of ug total) was loaded onto the column.
Long column life3
lysate proteins
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wash elute Re-equilibrate
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baseline expansion
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Reliable accuracy with large capacity
Key:Lane 1: Whole serum prior to separationLane 2: IgG standardLane 3: Peak 1 (flow-through fraction)Lane 4: Peak 2 (protein A-bound fraction; i.e. IgG1 and IgG2)
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Figure 4. Rapid human polyclonal IgG quantitation from human plasma using the Bio-Monolith Protein A column. IgG was bound and a 100% buffer B step gradient was applied; IgG eluted at 1.5 minutes.
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Injection numberFigure 3. Lifetime of the Agilent Bio-Monolith Protein G column is demonstrated over 1000 cycles of IgG3 binding and elution. Reproducibility of peak area enables accurate quantitation of the IgG3 titer.
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Learn more http://www.agilent.com/chem/ AdvanceBio
Order your Agilent Bio-Monolith Protein A and Protein G columns now at www.agilent.com/chem/store
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For Research Use Only. Not for use in diagnostic procedures. This information is subject to change without notice.
© Agilent Technologies, Inc., 2015Published in the USA, May 23, 2015 5991-5961EN
Ordering informationAgilent Bio-Monolith Protein A and Protein G ColumnsDescription Part NumberBio-Monolith Protein A, 4.95 x 5.2 mm 5069-3639Bio-Monolith Protein G, 4.95 x 5.2 mm 5190-6900
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