alaska king crab research.ppt

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    Alaska King Crab Research,

    Rehabilitation & Biology Program

    An Introduction

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    The AKCRRAB Science Team

    Ginny Eckert UAF/UAS

    Brad Stevens U. Mass. Dartmouth

    Brian Allee Alaska Sea GrantJeff Hetrick Alutiiq Pride Shellfish Hatchery

    Sara Persselin NOAA NMFS

    Celeste Leroux UAF/Alaska Sea GrantBen Daly Alaska Sea Grant

    Jim Swingle Alaska Sea Grant

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    Background Red King Crab Fishery

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    Background Blue King Crab Fishery

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    Early Enhancement Activities in Alaska

    1992 International King CrabRehabilitation and EnhancementSymposium

    1990s-2000s Extensive research

    by Dr. Brad Stevens in Kodiakand Japan

    2000s Continued research byNMFS Kodiak

    2006 Alaska Crab StockEnhancement and RehabilitationWorkshop

    Small scale experiment setup

    S. Persselin and B. Stevens, Kodiak AK

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    AKCRRAB - Structure

    Steering Committee

    Science Team

    Partnerships

    Research collaborators

    Advisory Board

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    Crab and Lobster Enhancement Initiatives-A Few Examples-

    Swimming crab, Japan

    Red King Crab, Japan

    American Lobster, New England

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    Crab and Lobster Enhancement Initiatives-A Few Examples-

    Blue crab, Chesapeake Bay

    Excellent model for

    investigating feasibility of

    enhancement

    European lobster, Norway Commercial sea ranching

    very successful

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    Prezoea

    Crab Instar 1Glaucothoe

    Zoea 4Zoea 3Zoea 1 Zoea 2

    Egg Day 1 Egg Day 395

    King Crab Life Cycle

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    King Crab Life Cycle

    Female with extruded eggsMating behavior

    Juvenile podding behavior Adult solitary behavior

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    Broodstock Collection

    St. Paul, Pribilof Islands

    November 2006Alitak Bay, Kodiak

    July 2006

    Anchorage

    Seward

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    Hatchery Setup

    Alutiiq Pride Shellfish HatcherySeward Marine Center

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    Hatchery Setup Larval culture

    Larval Rearing Modules at APSH

    Flow-through system Conical bottom tanks

    Circulation with air stones

    Uniform lighting of tanks

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    Larval Rearing Food Production

    Algae production

    24 hr. oldArtemia nauplius

    Artemia sp. production

    Isochrysis galbana algae

    Artemia sp. (brine shrimp) Algae

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    AKCRRAB 2007 Experiments

    Red King Crab Larval diet study

    Larval density

    hatchery trials

    Blue King Crab

    Larval diet/handling

    stress study

    Larval density study

    Juvenile trial rearing

    Water quality

    investigation P. Platypus ZIs swim in Petri dish

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    RKC Larval Rearing Diet StudyMarch 7, 2007 May 3, 2007

    Purpose:

    To test effect of algae or diatom enrichmenton RKC larval survival and growth toglaucothoe stage.

    Diet Treatments:

    Isochrysis galbana Tahitian strian (T-ISO)

    Pavlova lutheri(PAV)

    Thalassiosira pseudonana (3H)No enrichment,Artemia sp. only (Art.)

    P. camtschaticus ZI

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    RKC Larval Rearing Survival

    Percent Survival of RKC Larvae

    0%

    10%

    20%

    30%

    40%

    50%

    60%

    70%

    80%

    90%

    100%

    Z1 Z2 Z3 Z4 early G late G

    3H Art Iso Pav

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    BKC Larval Handling Stress/Diet studyMay 14, 2007 June 12, 2007

    Purpose:

    To test effects of live algae vs. algae

    concentrate as dietary enrichment

    and effect of handling stress on

    BKC larval survival and growth tothe glaucothoe stage.

    Treatments:

    Diet: Live T-ISOConcentrate T-ISO

    Handling: Once per zoeal stage

    No handling

    P. platypus ZIs

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    BKC Larval Rearing - Survival

    Percent survival per diet treatment

    -handling treatment only-

    0

    10

    20

    30

    40

    50

    60

    70

    80

    90

    100

    Z1 Z2 Z3 Z4 G

    MEAN LIVE MEAN CONCENTRATE

    Percent Survival of all BKC ZI to G

    0

    20

    40

    60

    80

    100

    Z1 G

    Handling, live algae No handling, live algae

    Handling, algae concentrate No handling, algae concentrate

    C & C G

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    RKC & BKC Growth

    Average dry weight of larvae

    0.000

    0.200

    0.400

    0.600

    0.800

    1.000

    1.200

    ZI ZII ZIII ZIV

    Weightinmilligrams

    RKC BKC

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    Average Temp. of RKC Larval Rearing Tanks

    3

    4

    5

    6

    7

    8

    9

    10

    Duration of experiment

    DegreesCe

    lcius

    Average temperature of BKC larval rearing tanks

    3

    5

    7

    9

    11

    13

    Duration of experiment

    DegreesCelcius

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    Larval Rearing Survival - Discussion

    Concern Course of Action

    Water Quality Monitor for hydrocarbons

    Microbiological contamination Monitor forVibrio bacteria

    Temperature fluctuation Improve water systemExcessive aeration Decrease aeration

    Nutritional deprivation Fatty acid analysis &

    Further diet investigations

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    Other Hatchery Studies

    Larval rearing density studies Water quality investigations

    Juvenile trial rearing

    Pathology & Genetics

    Larval fatty acid analysis

    Larval growth measurements

    Ongoing Projects

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    2007 Research Findings Summary

    Hatchery scale red and blue king crab larval and juvenilerearing is possible.

    Tremendous accomplishment in areas of technology

    transfer, hatchery setup, preliminary research and

    baseline data.

    Need to refine setup in identified ways to improve

    survival, as expected.

    P. camtschaticus glaucothoe

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    AKCRRAB Long-term vision

    Artificial Propagation

    Habitat Studies

    Genetics & Pathology

    Goal: Assess biological & economicfeasibility of red and blue king crab

    population rehabilitation through ocean

    ranching.

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    Thanks

    Alaska Sea Grant College Program

    AKCRRAB Science Team & Steering Committee

    NOAA Aquaculture Program

    NOAA National Marine Fisheries Service UAF School of Fisheries and Ocean Sciences

    Alutiiq Pride Shellfish Hatchery

    Alaska Department of Fish and Game

    United Fishermans Association Central Bering Sea Fishermans Association

    United Fishermans Marketing Association

    and all of our supporters nationwide

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    Questions

    R d ki b (P t h ti ) S d Al k