dna mutations and repair
Post on 25-Feb-2016
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DNA Mutations and Repair
Chapter 17
Detecting mutations with the AMES test
SUMMARY OF AMES TEST
AMES TEST
• Saccharin
• Aflatoxin
2-aminofluorene
Aflatoxin B1
DNA Repair: general characteristics
• Double stranded DNA• Redundancy of pathways
Types of DNA repair
• Mismatch repair
• Direct Repair
• Base-Excision Repair
• Nucleotide Excision Repair
Mismatch repair
• Mut H– Recognize GATC hemi-methylation
• Mut S• Mut L
A closer look at mismatch repair
Direct Repair
DIRECT REVERSAL REPAIR
BASE EXCISION REPAIR
BASE EXCISION REPAIR
Nucleotide Excision Repair
Diseases of DNA repair!
Xeroderma pigmentosumAutosomal recessive
Hereditary Non-Polyposis Colon Cancer
• HNPCC
• 15% of all colon cancers
Li-Fraumeni
• P-53 mutation
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