herbev presentation

Natural Preservation f Bof Beverages

with HerBev™

Challenge:

Microbiological spoilage of Microbiological spoilage of

lightly flavoured beverages.

Stabilization Methods

• Use of synthetic chemical preservatives

Stabilization Methods

y p(unwanted side effects eg hyperactivity, food sensitivity, risk of cross reaction eg benzene formation)

• Hot filling, pasteurisation and aseptic filling, paste ri ation cooling carbonation fillingpasteurization, cooling, carbonation, filling(residual yeasts can still be a challenge, also potential of contamination by end users on opening)contamination by end users on opening)

• Commercial sterilisation with HerBev™(simple dump and stir solution, on-going protection)

Properties of HerBev™• Composition: essential oils from culinary herbs and spices

p

• Regulatory status: GRAS, components widely used as flavours

• Appearance:Appearance:Concentrate: transparent light olive color, spicy odourIn use: colourless liquid, no flavour

• Label identification: natural flavours

• Ideal application: low pH, low particulate, pasteurised beverages

• Application rate 5 to 10ml/1000 litres (0.001%)

USP PET Challenge tests 1.gHerBev™ at 0.0005%, pH 4.5

Aspergillus Candida Pseudomonas Staphylococcus Escherichia p gniger

cfu/galbicans

cfu/gaeruginosa

cfu/g

p yaureus

cfu/gcoli

cfu/g

Initial 230,000 400,000 370,000 400,000 520,000Challenge

14 days 60,000 <10 <10 <10 <10

28 days 110,000 <10 <10 <10 <10

Method 3.38.3.1 3.38.3.2 3.38.3.3 3.38.3.4 3.38.3.5

The sample was found to PASS the antimicrobial effectiveness test according to the requirements of USP 31/NF for Category 2 products.

USP PET Challenge tests 2.gHerBev™ at 0.001%, pH 4.5

Aspergillus Candida Pseudomonas Staphylococcus Escherichia p gniger

cfu/galbicans

cfu/gaeruginosa

cfu/g

p yaureus

cfu/gcoli

cfu/g

Initial 230,000 400,000 370,000 400,000 520,000Challenge

14 days 25,000 <10 <10 <10 <10

28 days 10,000 <10 <10 <10 <10

Method 3.38.3.1 3.38.3.2 3.38.3.3 3.38.3.4 3.38.3.5

The sample was found to PASS the antimicrobial effectiveness test according to the requirements of USP 31/NF for Category 2 products.

USP PET Challenge tests 3.gHerBev™ at 0.002%, pH 4.5

Aspergillus Candida Pseudomonas Staphylococcus Escherichia p gniger

cfu/galbicans

cfu/gaeruginosa

cfu/g

p yaureus

cfu/gcoli

cfu/g

Initial 230,000 400,000 370,000 400,000 520,000Challenge

14 days 40,000 <10 <10 <10 <10

28 days <10 <10 <10 <10 <10

Method 3.38.3.1 3.38.3.2 3.38.3.3 3.38.3.4 3.38.3.5

The sample was found to PASS the antimicrobial effectiveness test according to the requirements of USP 31/NF for Category 2 products.

Recommendations from USP PET Challenge Tests:

• Pasteurization recommended (for cold formulations use Herbal-Active™)

• Maintain pH below 3.0 using glucono delta lactone and/or other organic acids eg citric, malicg g ,

• Maximize concentration of HerBev™ which imparts zero t t ithi th 5 t 10 l/1000 littaste within the range 5 to 10ml/1000 litres

• Add with concentrates, flavours, sweeteners and then dilute , ,to final volume

Some Benefits of Using HerBev™Some Benefits of Using HerBev

• no limitation to the type of low pH, pasteurized beverages

eg. carbonated / still, low / high juice content, sweetened /

unsweetened)

• no impact on taste odour or colour of treated beverages no impact on taste, odour or colour of treated beverages

• excellent effectiveness against yeasts

• no development of resistent germ-species

More Benefits of Using HerBev™

• compatible with all types of containment

More Benefits of Using HerBev

compatible with all types of containment

eg. glass, metal, plastic (PET, PVC), pure pack

•• no cost for dosing equipment, dump and stir solution

• comparable cost to benzoate, sorbate, metabisulphite

systems

• no environmental or negative health impactno environmental or negative health impact

• potential health benefits

i id i b i i C dideg. antioxidant, immune boosting, anti-Candida

Contact:

Cherikoff BioactivesA division of Vic Cherikoff Food Services Pty Ltd

functionals@cherikoff.net+61 2 9554 9477