the two-hybrid method for identifying and analyzing protein-protein interactions

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The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions. TAD. TAD. Sst2 interacts with Ste2 in a DEP-dependent manner in vivo. nucleus. Ub C-terminal hydrolase. NubG. HIS3. TAD. HIS3. TAD. HIS3. HIS3 encodes imidazoleglycerol-phosphate dehydratase - PowerPoint PPT Presentation

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The Two-Hybrid Methodfor Identifying and AnalyzingProtein-Protein Interactions

TAD

TAD

Ub C-terminal hydrolase

nucleus

Sst2 interacts with Ste2 in a DEP-dependent manner in vivo

NubG

HIS3

HIS3

HIS3

TAD

TAD

HIS3 encodes imidazoleglycerol-phosphate dehydratase[D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate hydro-lyase, EC 4.2.1.19]

His1 His4B His4A His6 His7 His3 His5 His2 His4C His4C

3-AT

H20

C

CH2—CH—COOH

NH2

L-histidine 3-aminotriazole

imidazole ring triazole ring

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