antibody-gated indicator releasing mesoporous...
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S1.2-AB
Cpermethrin / µg Kg-1
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CONTACT PERSON REFERENCES
ANTIBODY-GATED INDICATOR RELEASING MESOPOROUS MATERIALS: A POTENTIAL BIOSENSOR PLATFORM TO BE USED IN THE DEVELOPMENT OF RAPID TESTS
Estela Climent, Elena Costa, Kornelia Gawlitza, Wei Wan, Michael G. Weller, Knut Rurack Chemical and Optical Sensing Division, Bundesanstalt für Materialforschung und-prüfung (BAM
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MotivationKeeping in mind the high sensitivity offered by gated indicator-releasing micro- and nanoparticles due to their inherent features of signal
amplification, we embarked on system optimisation to develop a potential biosensor platform for use in rapid tests. Here, the insecticide
permethrin, a type-I pyrethroid, was selected as target analyte, because type-I pyrethroids play an important role in airplane disinfection.
Response in solution
1.- Analytical assay optimisation with S1.1-AB (FLU as dye)
200 nm 500 nm 200 nm S5S3 S4
Materials characterisation
Transmission electron microscopy (TEM)
N2 Adsorption/desorption isotherms and pore size distribution
Fig. 1 Increase in fluorescence at 522 nm (λexc = 490 nm) in the presence (red) and the absence
(black) of permethrin (6.6 mg kg–1) of the supernatants of S1.1-AB suspensions.
95: 5 10 mM PBS :
iPrOH,
95: 5 80 mM PBS : iPrOH, 90: 10
80 mM PBS : iPrOH,
Elemental (EA) and thermogravimetric (TGA) analysis
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S2
S3
S4
S5
pore diameter / nm
(dV
/dlo
gD
)/cm
3 g
–1
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S2
S3
S4
S5
P / P0V
ol. a
ds.
(cm
3 g
–1)
0.0010.01 0.1 1 10 100 1000
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0.3 permethrin
3-PBA
DF
/F0
cpyrethroid /µg kg–1
Fig. 2 Increase in fluorescence at 550 nm (λexc = 532 nm) in the presence (red)
and the absence (black) of 3-PBA (0.5 mg kg–1) of the supernatants of S1.1-AB
suspensions.
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S5.2a-AB(7 nm pores)
S2.2a-AB(9 nm pores)
S3.2a-AB(10 nm pores)
S4.2a-AB(17 nm pores)
S2.2b-AB(9 nm pores)
2.- Evaluation of pore size and different loadingroutes followed using SRG as dye
Solid C10%
µg Kg-1
AF
S1.1-AB 13 2.5
S1.2-AB 1 860
S2.2a-AB 2.5 1010
S2.2b-AB 4 197
S3.2-AB 4 12
S4.2-AB - -
S5.2-AB - -
EA TGA
FLU%
SRG%
HAPTEN %
PEG %
TOTAL%
TOTAL %
S1.1 6.6 – 2.0 – 8.6 9.4S1.2 – 29.4 2.0 – 31.3 29.6
S2.2a – 15.0 1.7 15.2 31.9 30.8S2.2b – 17.0 3.2 14.8 35.0 34.3S3.2a – 12.3 1.6 17.6 30.2 30.0S4.2a – 21.4 2.2 11.1 34.7 31.3S5.2a – 16.7 3.5 10.2 30.4 29.3
Signal amplification
factor
3.- Concentration-dependent studies (after 5 min of reaction)
Design and synthesis routes of materials
Antibody at the surface
Antibody inside of the pores
www.bam.de
Incorporation into differentsensing platforms
Microfluidic chips
Conclusions
✓ Increase in ionic strength and presence oforganic solvents accelerates release kinetics
✓ Indicator dye is decisive for the sensitivity
✓ Better material response is observed whensize of Fab region of the antibody is similar tothe pore diameter and hapten is grafted atthe entrance of the pores
Paper-based assaysDip-stick Lateral flow
Antibody inside of the pores(S2.2a-AB)
Similar sensitivity
SRG
Antibody at the surface(with different dyes)
FLU
1. E. Climent, A. Bernardos, R. Martinez-Manez, A. Maquieira, M. D. Marcos, N. Pastor-Navarro, R. Puchades, F. Sancenon, J. Soto, P. Amoros, J. Am. Chem. Soc 131 (2009) 14075.
2. J. Bell, E. Climent, M. Hecht, M. Buurman, K. Rurack, ACS Sens. 1 (2016) 334.3. E. Climent, D. Groninger, M. Hecht, M. A. Walter, R. Martinez-Manez, M. G. Weller, F. Sancenon, P. Amoros, K.
Rurack, Chem. Eur. J. 19 (2013) 4117.4. E. Costa, E. Climent, S. Ast, M. G. Weller, J. Canning, K. Rurack, Analyst (2020) DOI: 10.1039/D0AN00319K.5. E. Costa, E. Climent, K. Gawlitza, W. Wan, M. G. Weller, K. Rurack, J. Mat. Chem. B (2020) DOI:
10.1039/d0tb00371a.
Estela Climent Terol
Chemical and Optical Sensing DivsionBundesanstalt für Materialforschung und
–prüfung (BAM)