Newsflash April 2014

New Antibody Data Available

Cystatin C Fab Antibody

CRP Fab Antibody

Myeloperoxidase Fab Antibody

Our Catalogue

Monoclonal Antibodies

Polyclonal Antibodies

Recombinant Fab Antibodies

Quality InnovationResultsPartnership

Be confident in your results when you

choose BBI’s monoclonal antibodies for

your next assay:

Tested in ELISA

Tested in lateral flow

Recommended pairings provided

Strong customer feedback

We have now introduced highly sensitive

recombinant Fab antibodies to improve

your next generation assays.

Custom development and manufacturing

services to meet your individual

requirements also available.

Why choose BBI ?

Quick LinksRecombinant Fab

Technical Information >

Cystatin C Fab mAb >

CRP Fab mAb >

MPO Fab mAb >

Monoclonal Antibodies >

Polyclonal Antibodies >

Recombinant Fab

Antibodies >

How to Order >

What are the advantages of recombinant Fab

antibodies?

Standard Monoclonal AntibodiesRecombinant Fab Monoclonal

Antibodies

Initial Screen 100’s of clones screened 1,000,000,000 clones screened

Matched Pairing Best few antibodies tested for pairing >4500 antibody pairs tested

(Luminex platform)

Affinity micromolar as standard picomolar as standard

Sensitivity microgram/ml as standard picogram/ml as standard

Validation Purified Antigen Clinical samples

ConjugationMay interfere with antigen binding

region

Engineered Cysteine residue

ensures no interference

Production

Cell culture - slow, expensive,

unreliable Ascites - slow, uses many

animals, illegal in EU

E. coli - fast, reliable, easy to scale-

up, simple storage

Antigen binding sites per

mg of purchased productStandard 36% more

For more technical info visit our website >

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Example: Calcitonin recombinant Fab

antibody

Patients with calcitonin levels >100 pg/mL

have a high risk for medullary thyroid

carcinoma (~90%–100%) Toledo et al.,

(2009).

Our Calcitonin antibody pair detects at a

range of 6 to 2000 pg/mL.

These highly sensitive

antibodies are ideal for

evaluating risk of this carcinoma.

“Reduce your time and

money on reagents with

unproven performance – our

results speak for

themselves.”

See the demonstrated clinical relevance

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Antibody Spotlight NEW DATA – Cystatin C recombinant Fab antibody

Antibody Range of Detection*

BR175-G8D9 165 – 9073 ng/ml

BR175-H4C8 165 – 9073 ng/ml

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Can detect the presence of antigens down to 165 ng/ml.

Can be used in a competitive ELISA or Lateral Flow, without need for pairing.

Can be conjugated for use as a detection antibody.

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Cystatin C Antigen Concentration (µg/ml)

Competitive Lateral Flow Data forBR175-H4C8

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Cystatin C Antigen Concentration (µg/ml)

Competitive Lateral Flow Data forBR175-G8D9

*determined using ELISA, concentrations outside this range have not necessarily been tested .

Antibody Spotlight

NEW DATA – CRP recombinant Fab antibody

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0

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CRP conc (ng/mL)

Comparison of BR228-D4A3 with commercially available antibodies

BR228-D4A3

Competitor A

Competitor B

Flu Fab

Reader

CRP conc (ng/ml)100 75 50 25 10 5 2 1 0

BBI antibody, BR228-D4A3

Competitor B antibody

Competitor A antibody

Visual

Lateral Flow: Comparison of BR228-

D4A3 with commercially available

antibodies

Dynamic range of antigen detection of 151 - 23100ng/ml determined by ELISA.

Can be used in a competitive ELISA, without need for pairing.

BR228-D4A3 antibody, is able to detect down to < 2ng/ml in lateral flow allowing you to

produce high sensitivity assays.

Antibody Spotlight

NEW DATA – Myeloperoxidase (MPO) recombinant Fab

antibody

Recommended Sandwich Pairing

Capture Antibody Detection Antibody

BR240-N5G6 BR240-D8B4

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Can detect the presence of antigens at 2 – 1200ng/ml in ELISA.

They have now been tested in Lateral Flow.

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MPO Antigen (ng/ml)

Lateral Flow for BR240-D8B4 & BR240-N5G6

Order your evaluation samples

today:

info@bbisolutions.com

USA: 1-800-423-8199

Intl: +44 (0) 2920 747 232

Our Catalogue | Monoclonal Antibodies

Cardiac Infectious Disease Norovirus

CK-MB C. difficile Toxin A PBP2a

D-Dimer C. difficile Toxin B TB

Myoglobin Chikungunya

Troponin I Dengue NS1 Pregnancy

H. pylori AFP

Diabetes HAV

Hb HBV Tumour

HbA1c Hepatitis B CA125

HIV p24 CA15-3

Drugs of abuse HIV gp35 CA19-9

THCΔ9 HIV gp41 Calcitonin

Influenza A

Hormones Influenza B Immunoglobulins

DHEA-S Leptospira IgA

Cortisol Listeria IgE

Progesterone Malaria HRP2 IgG

PTH Malaria LHD (pan) IgM

Malaria LHD (Pf)

Malaria LDH (Pf & Pv) Return home >

Our Catalogue | Polyclonal Antibodies

Inflammation Pregnancy Renal

CRP AFP A2M

B2M

Hormones Inflammatory Cystatin C

HCG A1AT

αHCG

Haematology Metabolism

Immunoglobulins Haptoglobin Ceruloplasmin

IgA Ferritin

IgM Haemoglobin

IgG

Albumin

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Our Catalogue | Recombinant Fab Antibodies

* Detection Ranges are assay dependent and optimised for specific

clinical conditions, therefore may be improved with optimisation Return home >

Biomarker Detection Range* Capture Antibody Detection Antibody

HSP60 0.1 - 1200 ng/mL BR100-G2A7 BR100-H4C2

Adiponectin 0.1 - 525 ng/mL BR101-P7E2 BR101-M3H8

Adiponectin 230 - 59118 ng/mL BR101-H2B4 (Competitive ELISA)

Angiopoietin 2 0.001 - 97 ng/mL BR103-P4E8 BR103-V5F2

Big Endothelin 1 0.001 - 1.8 ng/mL BR104-D6A4 BR104-H1A4

Calcitonin 0.006 - 2 ng/mL BR388-G6B1 BR388-D5C4

Cathepsin D 0.03 - 150 ng/mL BR107-F5G2 BR107-K6D3

Eotaxin 0.007 - 2 ng/mL BR108-M7H3 BR108-K2D9

Chondrolectin 0.9 - 21 ng/mL BR111-P8G3 BR111-T6B1

CKBB 0.15 - 33 ng/mL BR112-V6F6 BR112-Z1C4

Proinsulin C-Peptide 0.09 - 38 ng/mL BR143-G5D3 BR143-K7C9

CRP 151 - 231000 ng/mL BR228-D4A3 (Competitive ELISA)

Cystatin C 165 - 9073 ng/mL BR175-G8D9 (Competitive ELISA)

Cystatin C 165 - 9073 ng/mL BR175-H4C8 (Competitive ELISA)

E. histolytica not tested BR116-K9F7 BR116-G3A1

E-selectin 0.05 - 235 ng/mL BR117-K2E7 BR117-H5B6

FABP Intestine 0.65 - 100 ng/mL BR115-F7G7 BR115-G6B9

FABP Liver 0.03 - 90 ng/mL BR106-G5A2 BR106-P9F5

Our Catalogue | Recombinant Fab Antibodies

* Detection Ranges are assay dependent and optimised for specific

clinical conditions, therefore may be improved with optimisation Return home >

Biomarker Detection Range* Capture Antibody Detection Antibody

G. lamblia not tested BR120-P3F2 & BR120-T1G8

Human Fetal Hemoglobin 1.9 - 305 ng/mL BR121-T5H9 BR121-V5A7

Leptin 3.9 - 691 ng/mL BR123-M4D6 BR123-P4G2

MMP7 0.1 - 293 ng/mL BR124-S4E3 BR124-T6G8

MMP9 Active 11 - 183 ng/mL BR125-N5F7 BR125-M6D6

MPO 2 - 1200 ng/mL BR240-N5G6 BR240-D8B4

Neutrophil Elastase 0.008 - 33 ng/mL BR127-H2A4 BR127-G7C2

Free PAPP A 0.3 - 299 ng/mL BR129-M5E3 BR129-F2H4

Pentraxin 3 4.6 - 876 ng/mL BR130-H2C3 BR130-K6D6

PGDS 0.2 - 85 ng/mL BR131-M6H7 BR131-P7F7

PSAP A 0.013 - 123 ng/mL BR132-T5G4 BR132-S6E4

PSAP B 0.3 - 1500 ng/mL BR133-V6B5 BR133-V4D1

RBP 4 350 - 500000 ng/mL BR134-Z7D3 (Competitive ELISA)

S100 Beta 0.04 - 5.6 ng/mL BR332-G4B9 BR332-F5H7

ST2 1.39 - 97 ng/mL BR139-S7B7 BR139-V8D4

ST2 0.08 - 31 ng/mL BR139-T3G5 BR139-V8D4

Tie2 0.8 - 600 ng/mL BR140-Z7A5 BR140-D9D7

How to Order

Please get in touch to arrange

a quote for any of our products.

info@bbisolutions.com

USA: 1-800-423-8199

Intl: +44 (0) 2920 747 232

Quote on ordering – NF APR

BBI Solutions

bbisolutions

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