atlas of light-ct images (biology and plants)

7/29/2019 Atlas of Light-CT images (biology and plants)

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LLTech

Light-CT Scanner

Atlas of images

Research applications


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TABLE OF CONTENTS

COSMETOLOGY Page 3

CELLS Page 8

DEVELOPMENTAL BIOLOGY Page 13

PLANTS Page 25

RESEARCH APPLICATIONS


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- In-vivo layer thickness measurement, cell count,epidermis/dermis junction analysis, for ageing

and hydration evaluation

- Skin model analysis

Back to Table of Contents

COSMETOLOGY


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Stratum corneum Stratum spinosum

Stratum basale Reticular region

Epidermis

Dermis

En-face skin slicing shows structural and cellular details

Melanin

papillary caps

Keratinocytenuclei

Blood vessels

Collagen

Corneocytes

100 m 100 m

100 m100 m

LLTech 2012 Courtesy of Hopitaux Universitaires de Genve, Genve, Switzerland


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Vertical reconstructions from en-face slicing:

Layer count and measurement

50 m

Stratum corneum

Stratum spinosum

Dermis

Human skin (in-vivo)

Skin model

Dalimier and Salomon Dermatology 224, 84-92 (2012)

Stack of images

-> vertical sections

Keratinocyte nuclei

Melanin caps at the

epidermis/dermis

junction

Blood vessel50 m

Evaluation of stratum

corneum thickness:

10 m


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Naevus imaging

500 microns

Dermal papillae

Hair follicule

100 m

50 m


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3D views

Wrinkle

Stratum corneum Stratum spinosum

Epidermis (separated by sodium bromide)

50 m

Reconstructed vertical slice

Dalimier and Salomon Dermatology 224, 84-92 (2012)

100 m

Corneocytes

Fingerprints (in-vivo)

Sweat duct


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- Cell count in native tissues and scaffolds

- Volume and shape analysis

CELL IMAGING



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Adipocytes in human tissue

9 m depth 27 m depth 60 m depth

Reconstructed

depth slice

200 m

50 m

200 m 200 m


10/3110 LLTECH 2011 Courtesy of Centre de Recherche des Cordeliers and ESPCI, Paris, France

Effect of fibrosis on adipocyte shape

Reconstructed vertical views of adipocytes in gel 3D modelling of adipocyte

No fibrosis

With fibrosis

50 m

50 m


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Cell scaffold

Scaffold en-face view

Scaffold reconstructed depth slice

100 m

100 m

Courtesy of University of Toronto, Canada

Hepatocytes nuclei are visible


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Individual cells

100 m

Courtesy of Universit de Reims, France

Isolate collagen cells


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- Time series analysis

- Reconstructed depth slices and 3D imaging

DEVELOPMENTAL BIOLOGY



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In vivo imaging of drosophila melanogaster:

automated time series over 72 hours

Prepupa (0-2 h)Transition to

pupal stage (24 h)

Pupal stage (48 h)Advanced pupal phase

before eclosion (72 h)

Courtesy of ESPCI, Paris, France


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100 um

In vivo imaging of drosophila melanogaster

4 days



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Otic vesicle

Cartilage

Xenopus Laevis

100 um

Pigmented cells

Courtesy of Muse National dHistoire Naturelle, Paris, France


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Xenopus Laevis: back/tail

100 um

30 m depth 60 m depth

MyoseptumEpithelial

cells

Neural

cells



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Xenopus Laevis

50 um

Reconstructed vertical slices from stacks of images



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Xenopus Laevis eye: en face slices

Surface 10 m depth 20 m depth 30 m depth

40 m depth 50 m depth 60 m depth 70 m depth

80 m depth200 um

230 m depth140 m depth 180 m depth



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Copepod

100 um

Courtesy of Laboratoire de Rgulation des Signaux de Division, EA4479, University of Lille


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Zebra fish eye

100 m

100 m

En face slices

Reconstructed depth slice

Courtesy of Inserm, Paris, France

10 m depth 20 m depth 45 m depth


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Zebrafish brain female, 1 year old

50 um

Courtesy of Laboratoire Hubert Curien, Saint Etienne

Reconstructed view from stack of images


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30 m depth

ovocytes

intestine

100 m

C. Elegans Fast identification of anatomy

Body wall - cuticle

gonad arm

uterus100 m

15 m depth

LLTech 2012 Courtesy of ENS, Paris, France

3D reconstruction


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- High resolution reconstructed depth slicesand 3D imaging

PLANTS



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Leaf veins fresh leaf sample

Fibers

Veins

100 m

500 m



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Apple

100 m

Wax

Skin

Flesh

500 m

Water



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Sorghum : cross section

Wide field en face

14 mm x 15 mm

Courtesy of Plate-forme d'Histocytologie et d'Imagerie Cellulaire Vgtale (PHIV), Montpellier RIO Imaging


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800 m x 800 m en face field

Courtesy of Plate-forme d'Histocytologie et d'Imagerie Cellulaire Vgtale (PHIV)Montpellier RIO Imaging

Sorghum cross section: native field view

Reconstructed

depth slices

500 m sample

thickness


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Rice: hypocotyle

Wide field en face

5mm x 2mm

Reconstructed depth slice, 200um penetration depth

Courtesy of Plate-forme d'Histocytologie et d'Imagerie Cellulaire Vgtale (PHIV), Montpellier RIO Imaging


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103 Carnegie Center Drive

Suite 300

Princeton, NJ 08540, USA

Phone: +1 609 995 3506

LLTech

Ppinire Paris Sant Cochin

29 rue du Faubourg Saint Jacques

75014 Paris

Phone: +33 1 82 72 61 25

www.lltechimaging.com

[email protected]

atlas of light-ct images (biology and plants)

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