audrey le gouellec laboratoire timc/the rex - umr 5525 · audrey le gouellec laboratoire timc/the...
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Audrey Le GouellecLaboratoire TIMC/The Rex - UMR 5525
Wh i b i f i h ?In vivo antigen delivery inside host cell
Why using a bacteria as vector for immunotherapy?g y
Potent immunostimulant (the « Danger theory »)
Knowledge in microbiology : bacterial physiology and engineeringKnowledge in microbiology : bacterial physiology and engineering
Forbes et al. 2010
A. Le Gouellec et al. 2012 Human vaccin and immunother.
« Killed but metabolically active »(KBMA) P. aeruginosa
Amotosalen
0µM 2µM 5µM 10µM 15µM 20µMEGTAEGTA
3h post infection LDH release
Systemic response in mouseCytokine assay xMAP Technology (Luminex)
DisseminationSPECT/CT experiment
J 14 J 7 J0
Prime Boost Challenge Tumoral
J-14 J-7 J0
Challenge Tumoral Vaccination
J0 J+1 J+24
ThanksThanksProf Benoit Polack
TheRExProf. Benoit Polack
TheRExExperimental recombinant therapy
TIMC-CNRS UMR 5525
Bertrand ToussaintXavier ChauchetCharlotte Genestet INSERM, U877
Jean-Luc LenormandCharlotte GenestetLionel BerthoinYan WangHichem Chaker
Mitra AhmadiAlexis BroisatCatherine GhezziHichem Chaker
David Laurin
ANRCEA/irTSV/BBSIJulien Verove ANR
INSERM, U823Caroline Aspord
Julien VeroveFrançois CretinIna AttreeCHU G bl
pJoel Plumas CHU Grenoble
IBP/UM BEPCandice Trocme