bbi solutions - human proteins
TRANSCRIPT
“With over 200 different proteins
available, we cater for all of your diagnostic
needs”
We offer both native and recombinant human proteins to ensure supply sustainability for our customers
Bulk capabilities – Available is sizes from 100ug to kg quantities
Quality systems - Operating to ISO 13485 we guarantee product quality, traceability and comprehensive documentation
Custom analysis - with global labs and hospitals, we can access many diverse testing platforms, providing you with the exact analysis results you need
Responsibly managed supply - over 25 years experience sourcing human biologicals at our HTA approved site
Bulk human protein production
Our human proteinsAcute Phase/Inflammation Markers Haptoglobin α-1-Acid Glycoprotein
C-Reactive Protein Ferritin- Liver Tumour Necrosis Factor Alpha
Hormones & Cytokines
FSH Growth Hormone hCG β-hCG Parathyroid Hormone (PTH) PAPP-A Prolactin SHBG
Renal Markers Albumin Cystatin C Lipocalin (NGAL)
RBP
α 1-Microglobulin (α1M) Tamm Horsfall
Glycoprotein Prealbumin
Immunoglobins IgA IgG IgM IgF Kappa Light Chain Lambda Light Chain
Tumour Markers Alpha Fetoprotein (AFP) Beta 2-Microglobulin
(β2M) CA125 CA15-3 CA19-9 CYFRA-21 PSA
PSA-ACT Complex PSA non Binding PAP Neuron specific Enolase Thyroid Peroxidase
Complement & clotting proteins C3c C4c Complement C1q D-Dimer Plasminogen
Cardiac Markers Apolipoprotein A1 (Apo
A1)
Apolipoprotein E (Apo E) Apolipoprotein B (Apo B) Beta-2 Glycoprotein 1 Creatine Kinase MB(CK-
MB) Creatine Kinase MM(CK-
MM)
High-Density Lipoprotein
Low-Density Lipoprotein
Myeloperoxidase (MPO)
Myoglobin
NT-Pro BNP
Troponin Complex
Troponin I
Troponin T
Infectious Diseases HIV TB
Malaria Clostridium difficile Toxin A PBP2a recombinant
protein
Plasma proteins Albumin
1 antitrypsin Butyryl Cholinesterase Alpha-2 macroglobulin Transferrin Transferrin Receptor HbA1c Ceruloplasmin
Autoimmune Markers Thyroxine Binding
Globulin Thyroglobulin Alpha 1 anti
chymotrypsin
>96% purity, minimising the risk of interferences due to contaminants
<3% dimer content ensuring the NGAL protein is highly specific for acute kidney injury
A faster response time, compared to other standard biomarkers
High quality, manufactured in the UK, under ISO 9001 Quality Management System
NGAL is a novel marker for the early diagnosis of acute kidney injury, our new product is available for sampling now and offers:
NEW – Recombinant Human NGAL
Highly specific to kidney injury
Results demonstrate that recombinant NGAL meets the required purity specification and is low in dimer
1. Novex Sharp Pre-stained Marker
2. Recombinant NGAL (non reduced sample)
1 2
New in 2015
Recombinant Galectin-3
Recombinant Procalcitonin
Recombinant Troponin I
and T
Recombinant from E.coli ≥ 96% pure Product available for
sampling
Troponins I and T levels become elevated in the blood within 3 or 4 hours after heart attack and may remain elevated for 10 to 14 days.
A novel Biomarker for chronic heart failure.
Increasingly used as a diagnostic marker for
various cancers.
From human cell line ≥ 95% pure Lyophilised Prototype samples
now available for evaluation
A promising marker for the early diagnosis of
clinically relevant bacterial infections and
sepsis.
≥ 96% pure Lyophilized From Escherichia Coli Prototype samples now
available for evaluation
Custom development and manufacture
Feasibility Optimisation Verification
Recombinant Screening of gene
databases E.coli & Pichia Cloning Transformation Small scale
fermentation
Native Sourcing of raw
materials of human origin
Small scale extraction and purification
Recombinant Optimised growth
conditions Formulation Downstream
processing Purification Fermentation capacity
of 30L per batch
Native Large scale extraction Downstream
processing Purification
Native & Recombinant HPLC Western blot Protein
electrophoresis IEF Gels Commercial
autoanalysers Stability trials Validation
Success story
Gene expression and purification of recombinant Lysin
The Challenge
A customer required two forms of recombinant Lysin however could not find these commercially and did not have the capabilities to manufacture them
The Solution
Our R&D team delivered the 2 variants to specification, timescale and budget
DNA Synthesis
Expression in bacteria
DNA Design Cloning Transformation Purification Characterisation
DNA gel