biology 22
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Biology 22. Drosophila Identification of various phenotypes. Outline. Brief description of Drosophila phenotypes and life cycle 1-Experiment 1: collection of data related to gender and body color 2-Experiment : 3 point Test Cross - PowerPoint PPT PresentationTRANSCRIPT
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Biology 22
DrosophilaIdentification of various phenotypes.
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Outline Brief description of Drosophila phenotypes and life cycle
1-Experiment 1: collection of data related to gender and body color
2-Experiment : 3 point Test Cross
How to study keep your flies still during the identification.
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Reference for Pictures:
Carolina Drosophila melanogster Manual (45-2620)
Published: Carolina Biological Supply Company
Based on research of Dr. Thomas Hunt Morgan
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1866-1945
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The life Cycle
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Life Cycle: Drosophila melanogaster
Eggs: contain filaments
2 molting phases “instars”
Life cycle is 10 days
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Fruity Fly Egg and Larva
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Fruit Fly Pupa and Adult
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1- EXPERIMENT: Identification of:
Gender
Body Color
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Wild Type Versus Mutants
Body Color
Wild type: BeigeMutant: Yellow body
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Parental phenotypes Experiment: body color
A= female: wt (beige) B=female: wt (beige) C=male: wt (beige) D=female: mutant (yellow) E=male: mutant (yellow)
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Fruit Fly Body Color
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Sex combs: Male Drosophila
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Female and Male Fruit Flies
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Abdominal segements
7 segments to female abdominal segments
5 segments to male abdominal segments
Male body size is smaller than female
But the “last” abdominal segment is larger in male
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2- Experiment: 3 point test cross:
Experiment 2 3 point test cross
Eye color Body color Wing length
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3 point test cross for:
1. Body Color
2. Wing length
3. Eye Color
♦ Wt vs. Mutant
Beige (bl+) vs. Black (bl)
Long(vg+) vs. Vestigal(vg)
Brick red (br+) vs. brown (br)
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Crosses:
Female Males
Males and female progeny from this F2 cross are your 3 point test cross results!
Xbl vg bw
bl vg bw
bl+ vg+ Bw+
bl vg bw
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There are all sorts of eyes!
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Eyes
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You will have only red eyes (wt)
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Fruit Fly Wings
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Nap TimeTriethylamine
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How to anesthetize a fly
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How to use the Napper
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View the Fruit Fly
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You will record class data
After counting the crosses record the data on the class spreadsheet
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