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The Origin and Early History of Life Cellular organization Response to stimulus Reproduction Regulation/ metabolism Homeostasis Heredity Growth and development Fundamental Properties of Life-Remember These?

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Page 1: Cellular organization Response to stimulus Reproduction Regulation/metabolism Homeostasis Heredity Growth and development Fundamental Properties of Life-

The Origin and Early History of Life

•Cellular organization•Response to stimulus•Reproduction•Regulation/metabolism

•Homeostasis•Heredity•Growth and development

Fundamental Properties of Life-Remember These?

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Biogenesis vs. Spontaneous Generation

Spontaneous Generation = principle that a living organism can arise from non-living material

Biogenesis = principle that a living organism can arise only from other living organisms

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Disproving Spontaneous Generation: Experiments

1.) Francesco Redi (1627-1697)-physician & naturalist Fresh meat in a jar w/ no cover =

MAGGOTS! Fresh meat in a jar w/ cover = no

maggots Conclusion: maggots came from flies

that were able to get to the meat, NOT DIRECTLY FROM THE MEAT!

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Disproving Spontaneous Generation: Experiments

( Francesco Redi Experiment Continued)

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Disproving Spontaneous Generation: Experiments

2.) John Needham (1713-1781) – biologist and priest Warmed up gravy and sealed it =

microorganisms grew!▪ What happened?!▪ He thought = a higher being caused

spontaneous generation to occur▪ Future scientists found = Needham did not

boil his sample and didn’t kill the microorganisms

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Disproving Spontaneous Generation: Experiments

3.) Lazzaro Spallanzani (1729-1799)-naturalist & priest

Boiled decaying matter & sealed it = no microorganisms

Why boil? Kill any microorganisms present

Conclusion: life does not spontaneously form from decaying matter

Critics claimed that microorganisms could not breathe because of lack of oxygen.

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Disproving Spontaneous Generation: Experiments (Lazzaro Spallanzani Experiment Continued)

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Disproving Spontaneous Generation: Experiments

4.) Louis Pasteur (1822-1895)-microbiologist

Boiled meat with broth in a flask Allowed air in, but no

microorganisms = no life Allowed air & microorganisms in =

LIFE! Conclusion: life must be present for

new life to form

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Disproving Spontaneous Generation: Experiments (Louis Pasture Experiment Continued)

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Early Earth

Evidence that the Earth formed 4.6 billion yrs ago Many meteorites Original conditions could not support life as we know

it. Early atmosphere contained lots of molecules but was void

of oxygen gas ▪ Evidence of no free oxygen: no iron oxide (rust) in oldest rocks▪ Molecules of Early Earth: H2O (water), H2 (hydrogen gas), CO2

(carbon dioxide), CO (carbon monoxide), N2 (nitrogen gas), CH4 (methane), NH3 (ammonia)

No free O, O2, or O3 (ozone)!

Intense UV radiation due to lack of O3 = no ozone layer Violent thunderstorms Frequent volcanic eruptions

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Before Life

Scientists believe life on Earth is a product of itself

Elements combined to form many molecules, including complex organic ones = MACROMOLECULES! (RNA, DNA, proteins, carbohydrates, and lipids) Ribozymes – self-replicating RNA (has

enzymatic properties but is a nucleic acid)

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Miller- Urey Experiment Miller-Urey Experiment

- Stanley Miller & Harold Urey, 1950s

attempted to reproduce early reducing atmosphere and produce organic compounds from inorganic materials

Built a contraption simulating the early Earth (methane, ammonia, water, hydrogen, lightning, heat, etc)

After 1 week = amino acids, sugars, lipids & other organic molecules

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Miller-Urey Experiment

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Water

Aldehydes

Proprionic acid

Lacticacid Glycolic

acid

Succinicacid

Glycine

Alanine b-Alanine

b-Aminobutyric acid a-Aminobutyric acidN-Methylalanine

Valine

Proline

Aspartic acid

Glutamic acid

Rawmaterials

First group ofintermediate products

Second group ofintermediate products

Examples of final products(isomers are boxed)

Immunoacetic propionic acid

Iminodiacetic acid

Norvaline Isovaline

Sarcosine

Acetic acid

Formic acid

N-Methylurea

Urea

Energy Energy

Hydrogencyanide

Nitrogen

Ammonia

Carbondioxide

Carbonmonoxide

Methane

Hydrogengas

Energy

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First Life On Earth

Around 3.8 BYA, the earliest, simplest forms of life are thought to have been formed

Cells with few organelles (prokaryotic cells) did not use oxygen to live= this is called fermentation. They are anaerobic organisms …

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Anaerobic vs Aerobic Respiration All Respiration: used to release energy from

the food we consume What do we use that energy for?

Anaerobic Organisms can release energy without oxygen Produces a small amount of energy = inefficient Like the first Prokaryotes (simple cells, few

organelles)- Did NOT use oxygen for respiration Aerobic organisms are able to release energy

with oxygen Produces a large amount of energy = very efficient Eukaryotes (complex cells, with lots of organelles) ▪ Ex: Mitochondira, Chloroplast, etc.

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From Prokaryotes to Eukaryotes

Environmental factors caused changes/mutations in DNA allowing some cells to use the sun to make their own food= Autotroph. (Similar to the organelle…?) Photosynthesis = takes out carbon dioxide and

creates oxygen in the atmosphere!!!!

As many anaerobic prokaryotes were killed off due to the production of oxygen in the atmosphere…

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From Prokaryotes to Eukaryotes More changes/mutations in cells allowed

them to use the oxygen to release energy (Similar to what organelle…?) Oxygen gathering in the atmosphere allows

oxygen-using organisms to evolve= aerobic prokaryotes survived

Oxygen gathering in the atmosphere allows oxygen-using organisms to evolve=▪ The organisms use Aerobic Respiration are more

efficient cells

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What happens when a cell eats?

Usually when a single-celled organisms eats, it will digest the food and then release the rest as waste.

THANKFULLY, somehow something went wrong… Which gave rise to Eukaryotic cells

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From Prokaryotes to Eukaryotes

Aerobic prokaryotes survived Larger prokaryotes ate smaller

aerobic & photosynthetic prokaryotes = EUKARYOTES! endosymbiotic theory(Trash bag

Demo)

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From Prokaryotes to Eukaryotes

[Endo = "within"] [symbiosis = “together”]

Endosymbiosis = Each single cell was engulfed by a single, larger prokaryotic cell at some point- Not digested becomes a part of the cell

Endosymbiotic Theory = “mitochondria-like” prokaryotes were free living (did

aerobic cellular respiration = used oxygen) “chloroplast-like”prokaryotes were free living (did

photosynthesis = used sunlight) These single free-living prokaryotic cells became

organelles of other larger cells; they formed a symbiotic relationship = endosymbiosis = first eukaryotic cells

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22https://www.youtube.com/watch?v=-FQmAnmLZtE

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Evidence for the Endosymbiotic Theory

Lynn Margulis researched in 1960's, published in 1981▪ proposed the endosymbiotic theory based on:

1) Mitochondria/Chloroplast have their own DNA similar to prokaryotic DNA

2) Mitochondria/Chloroplast divide like prokaryotes independent of the rest of the cell

3) Mitochondria/Chloroplast have two membranes4) Mitochondria/Chloroplast have simple ribosomes that are

the same size and structure as in prokaryotes5) Appearance of fossils, could best be explained by "endo-

symbiosis".

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Early Life

Around 2 BYA, slightly more complex organisms (heterotrophic eukaryotes) evolved Heterotrophs= organisms that have to eat/consume

other organisms for food/energy

Next to evolve were the colonial, unicellular eukaryotes that worked together multicellularity

Organisms moved from water about 450 MYA onto land – terrestrial life!

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Bacteria Archae-bacteria

AnimaliaFungiProtista Plantae

4500

4000

3500

3000

2500

2000

500

1500

0

1000

Formation of earth

Molten- hot surface ofearth becomes somewhatcooler

Oldest definite fossilsof prokaryotes

Appearance of oxygenin atmosphere

Oldest definite fossilsof eukaryotes

First multicellularorganisms

Appearance of animalsand land plants

Colonization of landby animalsPaleozoic

Mesozoic

Cenozoic

Mill

ion

s o

f y

ears

ag

o

AR

CH

EA

N PR

EC

AM

BR

IAN

PR

OT

ER

OZ

OIC

PH

AN

ER

OZ

OC

Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.

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Geologic Time

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1,900 2,500

4, 600

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Eras:Time

Geologic Time: begins when the Earth began 4.6 billion years ago

Includes: Precambrian Time :Archean and Proterozoic

Eras (4.6 billion years ago to 570 million years ago) ▪ Precambrian Period covers ~88% of Earth’s history▪ Made up of 2 eras- Archean and Proterozoic

Paleozoic Era (570 MYA to 225 MYA) Mesozoic Era (225 MYA to 65 MYA) Cenozoic Era (65 MYA to present) = TODAY!

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ErasWhat Came Into Existence?

▪ Archean-▪ Proterozoic-

▪ Paleozoic- Protists, sponges, and jelly fish to insects and amphibians and early reptiles; Bryophytes, mosses, and ferns (life move from sea to land)

▪ Mesozoic- Early mammals, early flowering plants, dinosaurs and other reptiles dominate

▪ Cenozoic- Mammals began to dominate, primates and humans arose (Primative horses Modern horses)

Single celled organisms (prokaryotes and protists), fungi, and simple invertebrates

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Geologic Time and Relative Dating

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Review

Geologic Time: begins when the Earth began 4.6 billion years ago

Includes: Precambrian Time :Archean and

Proterozoic Eras (4.6 billion years ago to 570 million years ago)

Paleozoic Era (570 MYA to 225 MYA) Mesozoic Era (225 MYA to 65 MYA) Cenozoic Era (65 MYA to present) =

TODAY!

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Fossils

Fossils Preserve only the hard parts usually Burgess Shale- quick reading Traces of Organisms▪ Tracks, footprints, burrows▪ Imprints of leaves or fish which shows the surface

features; shells leave molds of empty cavities; sand or mud fills a mold producing a cast (replica of original)

▪ Waste material = castings, eating habits of animals

▪ Stones in digestive system (dinos)

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Fossil Record

▪Shows evidence for continental drift▪Shows evidence for mass extinction▪Shows evidence for evolution (slight changes in morphology = the way something looks)▪Fossils can be dated by looking at the layer of rock they are in compared to other layers = RELATIVE DATING

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Mass Extinctions

Mass Extinction: A large number of species on Earth are eliminated due to a drastic change in the environment or catastrophic event occurs Considered a MASS EXTINCTION when at least 50%

of all of the species die out Each Era ended with a mass extinction (sort of) as

well as many other extinctions throughout Earth’s history▪ Extinction with the biggest impact on life was the Permian

Extinction that wiped out 95% of all species Scientists predict there will be a 6th mass extinction

to end this era… Yikes =(

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Relative Dating

Relative Dating: placing rocks (containing fossils) in their proper SEQUENCE OF FORMATION; compare one rock to another rock by examining their placement in the Earth; compare 1st, 2nd, 3rd, etc. The layer on top is the youngest (unless

it has been turned over) The layer below is older (unless it has

been turned over)

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Example: Place order of rocks from oldest to youngest.

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Answers… oldest to youngest

H G F Fault D Intrusion

(C) B E A Stream

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Absolute Dating and the Fossil Record

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Absolute Age

Absolute Age: actual age of the rock Radioactive Decay: isotopes that emit

particles and energy at a constant rate from their nuclei▪ Change to new isotope of same element or

new element all together▪ Natural Clocks = measure how much original

isotope is present compared to how much new isotope is present

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Example - Uranium

Example: Uranium emits 2 protons and 2 neutrons =

RADIOACTIVE DECAY▪ Uranium – parent element (unstable)▪ Lead – daughter element (stable)

Half-Life: time required for half of any given amount of radioactive material to decay into the daughter elements U-238 to decay into Pb- 206 is 4.5 billion years Example: 10 g of U- 238, after 4.5 billion years = 5 g

of U-238 and 5 g of Pb-206 After another 4.5 billion years – 2.5g of U-238 and 7.5

g of Pb-206

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Carbon Dating

Carbon Dating: more recent samples = carbon – 14 decays to nitrogen – 14 Usually C-12 and C-14 are constant, but

after death, C-12 and C-14 are not taken in, so amount of C-14 remaining breaks down to N-14

Half-life of C-14 is 5,730 years – find age of organic material (early humans, wood, bones, shells)

Ages up to 70,000 years old