does spatial distribution of boutons relate to orientation preference in layer iv spiny neurons?
DESCRIPTION
Does spatial distribution of boutons relate to orientation preference in layer IV spiny neurons?. Fuyuki KARUBE and Zoltán F. Kisvárday Laboratory for Cortical Systems Neuroscience, Medical and Health Science Center (MHSC), University of Debrecen, HUNGARY. Materials and Methods. - PowerPoint PPT PresentationTRANSCRIPT
Does spatial distribution of boutons relate to orientation preference in la
yer IV spiny neurons?
Fuyuki KARUBE and Zoltán F. KisvárdayLaboratory for Cortical Systems Neuroscience,
Medical and Health Science Center (MHSC),
University of Debrecen, HUNGARY
Materials and Methods
• Adult cats (2.3 - 3.2 kg) were used. • Visual stimuli
Moving luminance gratings (square waves; frequency, 0.1-0.8 cpd; velocity, 2 Hz).
• ImagingCortex (A18) was illuminated at 605 nm light. Int
rinsic signals were captured with a CCD camera. • Extracellular recordings to identify receptive field properti
es.• Tracer injections for extracellular staining• Reconstruction of stained single cells by Neurolucida.
Time course of intrinsic signal
From Grinvald and Bonhoeffer
Start visual stimulation
Stop stimulation
Arb
itrar
y un
its
Time after stimuli onset (s)
Activity dependent changes in [oxyHb] and [deoxyHb]
Optical imaging of intrinsic signal
From Grinvald and Bonhoeffer
Avtivated area by vertical gratings
Analysis of map structure for orientation preference
Orientation preference patches elongated along iso-elevation line in visual field
Reconstructions of layer IV spiny neurons
Dendrites were drawn in black, axons in red.
Bouton distribution pattern relative to orientation preference
Within 400m diameter
Local
Distal
Bouton distribution in iso-,oblique-, and cross-orientation patches
Tentative LM and AP-elongated cells on orientation preference map
Quantification of bouton distribution
Bouton distribution to cortical dimensions and preferred orientations
conclusion
• Layer IV spiny neurons showed various bouton distribution from cell to cell.
half of them preferred iso-orientation, another half did cross-orientation.
• Orientation patches elongated with LM (or iso-elevation) axis. Thus, spatial distribution of boutons of single cells can affect connectivity between iso- or cross-orientation preference patches.
Tangential viewCoronal view