bp
Lanes indicated with M: molecular size markers (100 base pair ladder (Pharmacia Biotech, Uppsala, Sweden).
Figure 1. ARDRA patterns obtained after restriction of the amplified 16S rDNA with CfoI. 1: pattern 1, Corynebacterium afermentans subspecies afermentans (CIP 103499T)2: pattern 2, CDC coryneform group ANF 1-like (IBS T30014)3: pattern 3, C. minutissimum (ATCC 10288T)4: pattern 4, C. urealyticum (ATCC 43042T)5: pattern 4+, CDC coryneform group ANF 3-like (IBS T88848)6: pattern 5, C. amycolatum (CIP 103452)7: pattern 6, C. pseudotuberculosis bv. equi (CIP 5297)8: pattern 7, CDC coryneform group B-1 (IBS T94631)9: pattern 8, CDC coryneform group B-3 (IBS T95303)(Pattern 9 (coryneform group B-3 (IBS T79396) : not shown)
Figure 2. ARDRA patterns obtained after restriction of the amplified 16S rDNA with AluI. Lane numbers correspond with pattern numbers: 1: Corynebacterium afermentans subspecies afermentans (CIP 103499T)2: C. afermentans subsp. lipophilum (CIP 103500T)3: C. diphtheriae (CIP 100721T)4: C. jeikeium (CIP 103337T)5: C. pilosum (ATCC 29592T)6: C. propinquum (CIP 103792T)7: C. cystitidis (CIP 6940)8: 'C. macginleyi' (IBS B72932)9: CDC coryneform group F-1, genomospecies 1 (IBS B1657)10: C. pseudotuberculosis bv. ovis (CIP 102968T)11: C. renale (CIP 6937)12: CDC coryneform group ANF3-like (IBS T88848)13: CDC coryneform group B-1 (IBS T94631)14: CDC coryneform group B-3 (IBS T79396)15: Rhodococcus equi (CIP 5472T).
Figure 3. ARDRA patterns obtained after restriction of the amplified 16S rDNA with RsaI. 1: pattern 1, C. afermentans subsp. lipophilum (CIP 103500T)2: pattern 2a, C. diphtheriae (CIP A 102)3: pattern 2b, C. pseudotuberculosis bv. equi (CIP 5297)4: pattern 2c, C. pseudotuberculosis bv. ovis (CIP 102968T)5: pattern 3, C. propinquum (IBS B50121)6: pattern 4, C. striatum (CDC E 8873)7: pattern 5, C. urealyticum (ATCC 43043)8: pattern 6, C. xerosis (IAM 12431)9: pattern 7, CDC coryneform group G, genomospecies 1 (LCDC 81613)10: pattern 8, CDC coryneform group F-1, genomospecies 1 (IBS B1657)11: pattern 9, CDC coryneform group ANF 3-like (IBS T88848)12: pattern 10, C. renale (CIP 103421T)13: pattern 11, CDC coryneform group B-3 (IBS T95303)14: pattern 12, CDC coryneform group B-3 (IBS T79396)15: pattern 13, Rhodococcus equi (CIP 5472T)
Species Abbreviation Alu I Cfo I Rsa ICorynebacterium afermentans subsp. afermentans (CDC ANF-1) afa 1 1 1C. afermentans subsp. lipophilum (CDC ANF-1) afl 2 1 1C. jeikeium genomospecies A (former JK) jei 4 1 1 genomospecies C 4 1 1'C. macginleyi' mac 8 1 1C. pseudotuberculosis biovar ovis pto 10 1 2cC. renale ren 11 1 10CDC coryneform group ANF-1-like A1l 1 2 1C. diphtheriae dip 3 2 2aC. propinquum (CDC coryneform group ANF-3) pro 6 2 3C. pseudodiphtheriticum pse 6 2 3C. cystitidis cys 7 2 6CDC coryneform group G (genomospecies 2) G2 8 2 3C. accolens acc 8 2 1CDC coryneform group F-1 (genomospecies 1) F1 9 2 8C. striatum str 3 3 4CDC coryneform group G (genomospecies 1) G1 3 3 7C. pilosum pil 5 3 1C. minutissimum min 3+ 3 1C. urealyticum (former D2 of JK-like) ure 4 4 5C. xerosis xer 7 4 6Rhodococcus equi Req 15 4 13C. amycolatum CDC group F-2 amy 7 5 6 'C. asperum' 7 5 6 CDC group I-2 7 5 6 biovar equi pte 10 6 2bCDC coryneform group B-1 B1 13 7 12CDC coryneform group B-3 B3 14 8 11CDC coryneform group B-3 B3 14 9 12CDC coryneform group ANF-3-like A3l 12 4+ 9
ARDRA identification scheme of coryneforms
