03-1
8 /
9312
875
006
GB
A /
© b
ioM
érie
ux •
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men
t and
/or p
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not l
egal
ly b
indi
ng. M
odifi
catio
ns b
y bi
oMér
ieux
can
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ithou
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• BI
OM
ERIE
UX, t
he B
IOM
ERIE
UX lo
go, A
SAP,
DIL
UBA
G, I
BISA
and
SAL
MA
are
used
pen
ding
and
/or r
egis
tere
d tr
adem
arks
bel
ongi
ng to
bio
Mér
ieux
, or
one
of i
ts s
ubsi
diar
ies
or o
ne o
f its
com
pani
es. A
ny o
ther
nam
e or
trad
emar
k is
the
prop
erty
of i
ts re
spec
tive
owne
r. •
Phot
os: b
ioM
érie
ux, N
. Bou
chut
, Shu
tter
stoc
k •
bioM
érie
ux S
.A. R
CS L
yon
673
620
399
• Pr
inte
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Fra
nce
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SALMA®UNIQUE MEDIA
Detection and Confirmation of Salmonella spp.
ORDERING INFORMATION
SALMA®
bioMérieux S.A. • 69280 Marcy l’Étoile • France • Tel.: + 33 (0)4 78 87 20 00 • Fax: +33 (0)4 78 87 20 90 www.biomerieux.com • www.biomerieux-industry.com
FOOD SAFETY
DECIDEWITHCONFIDENCE
SALMA®
4704690 418246418247
SALMA® One Day Kit, 120 testsSALMA® 120 plates Ø 90 mmSALMA® 20 plates Ø 90 mm
SALMONELLA SUPPLEMENT421202421203
Salmonella Supp Tab 25gSalmonella Supp Tab 375g
CONFIRMATIONMGNF42AEB526760
Salmonella spp. latex agglutination test, 50 testsSALSA™ 20 bi-plates Ø 90 mm
BUFFERED PEPTONE WATER
4204242043AEB610309LAEB910303/4AEB910305/242729421121421120421122
6 bottles 90 ml6 bottles 225 ml6 bottles 900 mlDILUBAG™ BPW 4 X 3LDILUBAG™ BPW 2 X 5LMiniBag BPW 10 x 225 mlBuffered Peptone Water 500 gBuffered Peptone Water 5 kgBuffered Peptone Water 10 kg
Please contact us for more detailed information
NB FOOD CULT-SALMA.indd 2-3 08/03/2018 12:17
Compliant with the CE 2073/2005 regulation
(modified by the 1441/2007)
DE
TECTION
E N U M E R AT I ON
ISO 16
140 VA LI DATIO N
ISO 16140VALI DATION
ENUMERATION
V A L I D A T I ON
ISO 16140
OFFICIAL
VALIDATION
AOAC VALIDATION
ISO 16
140 VA LI DATIO N
OFFICIAL
VALIDATION
AOAC VALIDATION
D E T E C T I O N
AOAC RI & ISO 16140
V A L I D A T I O N
DETECTION
V A L I D A T I ON
ISO 16140
CONFIRMATION
V A L I D A T I ON
ISO 16140
ENUMERATION
V A L I D A T I ON
ISO 16140
DETECTION
V A L I D A T I ON
AOAC RI & ISO 16140
ENUMERATION
V A L I D A T I ON
AOAC RI & ISO 16140
DETECTION
V A L I D A T I ON
AOAC RI & ISO 16140
DETECTION
E N U M E R AT I ONVALI DATI O N
ISO 16140
SALMA®Unique and innovative method• Easy, Flexible and innovative supplementation• Detection of motile and non motile Salmonella, lactose+ Salmonella and other atypical serotypes within 48hrs (enrichment included)
• ISO 16140 validation for food stuffs, animal feeding stuffs and environmental samples with no restrictions of use
Salmonella spp.: pink to purple coloniesOther Enterobacteriaceae: blue or white colonies
24 hour protocolSALSA™
Rapid protocol Agglutination latex test
C8 esterase+ strain(pink to purple
colonies)
Lactose+(yellow colonies and H2S-)
Slow C8esterase strain
LDC+ and H2S+(red colonies with black centre)
LDC+ and H2S+(red colonies with black centre)
C8 esterase+ strain(pink to purple
colonies)
High selectivity and specificity• Mix of chromogenic susbtrates targeting the esterase activities of Salmonella
• High differentiation between Salmonella and the other enterobacteria• Absence of Salmonella spp.: blue or white colonies• Presence of Salmonella spp.: pink to purple typical colonies• High inhibition of the interfering flora thanks to the combination
of an enrichment selective supplement and a highly selective chromogenic culture medium
Benefits• Simple and convenient protocol: 1 bag/1 plate method with easy
supplementation• Easy to read thanks to the colors contrast: pink to purple
colonies on white agar • Flexibility and workflow optimization: Enrichment bags and
plates can be held up to 72 hours in refrigerated conditions• Traceability of the supplementation by color change of the BPW
Simple and rapid confimation2 validated solutions are available: • Rapid Protocol: results within a few minutes thanks to
an agglutination latex test • 24 hour protocol: isolation on SALSA™ plate (ASAP™/XLD) and
incubation 24 ±2hrs at 37 ±1°C
YOUR SOLUTION FOR SALMONELLA DETECTIONHighly selective method for the detection of motile and non motile Salmonella spp. in food and environmental samples
UN
IQU
E M
EDIA
X g or X ml of sample in 9X ml of BPW
Absence of Salmonella spp.(no colonies, blue or white colonies)
Presence of Salmonella spp.(typical colonies: pink to purple)
Incubation 16 to 24h at 41.5±1°C
CONFIRMATION
OR
Streak 10µL on SALMA® plate
Incubation 21 to 27h at 37±1°C
Add Salmonella Supp Tab
D0
Enrichment
D1
Isolation
D2
Reading and result
BPM
BPW + Salmonella Supp Tab SALMA
RVS & MKTT XLD & ASAP Confirmation test Nutrient ISO agar+ biochemical galleriesISO 6579
1st enrichment
SINGLE enrichment +Latex confirmation
2nd enrichment
Screening in ONLY 2 days!(including confirmation)
Cost & time saving method ➤ 3 days saved !
Screening in 5 days
D1 D2 D3 D5
WORKFLOW COMPARISON: SALMA ONE DAY VS. ISO 6579
SALMA®
NB FOOD CULT-SALMA.indd 4-5 08/03/2018 12:17
bioMérieux S.A. • 69280 Marcy l’Étoile • France • Tel.: + 33 (0)4 78 87 20 00 • Fax: +33 (0)4 78 87 20 90www.biomerieux.com • www.biomerieux-industry.com01
-18
/930
7722
006
/GB/
B /
© b
ioM
érie
ux •
Doc
umen
t and
/or p
ictu
res
not l
egal
ly b
indi
ng. M
odifi
catio
ns b
y bi
oMér
ieux
can
be
mad
e w
ithou
t prio
r not
ice
• BI
OM
ERIE
UX, t
he B
IOM
ERIE
UX lo
go, A
PI, C
hrom
ID, D
iluba
g, R
apid
ec a
nd V
idas
are
use
d pe
ndin
g an
d/or
regi
ster
ed tr
adem
arks
bel
ongi
ng to
bi
oMér
ieux
, or o
ne o
f its
sub
sidi
arie
s or
one
of i
ts c
ompa
nies
. Any
oth
er n
ame
or tr
adem
ark
is th
e pr
oper
ty o
f its
resp
ectiv
e ow
ner.
ALOA
is a
regi
ster
ed tr
adem
ark
of B
iolif
e S.
r.l..
Accu
Prob
e is
a re
gist
red
trad
emar
k of
Hol
ogic
Inco
rpor
ated
. bi
oMér
ieux
SA
673
620
399
RCS
Lyon
/ P
hoto
s: b
ioM
érie
ux,
SHAS
HIN
KO
UBO
U H
YPER
LIN
K, N
. Bou
chut
/ P
rinte
d in
Fra
nce
/ th
éra
398
160
242
RCS
Lyon
.
ALOA®UNIQUE MEDIA
Detection, Enumeration and Con� rmation of Listeria spp. and Listeria monocytogenes
YOUR CONFIRMATION SOLUTIONS
ORDERING INFORMATION DETECTION ENUMERATIONL. mono L. spp. L. mono L. spp.
AEB520080AEB520079AEB120082AEB620088AEB150072AEB150073AEB684420AEB484204AEB120100
ALOA® 20 plates Ø 90 mmALOA® 120 plates Ø 90 mmALOA® 10 plates Ø 140 mm
ALOA® Count 5 x 180 ml (base + suppl)ALOA® BASE 500 g
ALOA® BASE 5 kgALOA® SUPPLEMENT 6 x suppl 500 ml base
ALOA® SUPPLEMENT 4 x suppl 4L baseALOA® Con� rmation 10 plates Ø 90 mm
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037001030030704415078
Rapidec® Lmono (20 tests)API® Listeria
VIDAS® LMO2Fast Rhamnose (30 tests)
●
●
●
●
●
●
●
●
●
●
AEB110101416477AEB522050
BHI 20 tubes 1 mlListeria spp con� rmation strips
PALCAM 20 plates Ø 90 mm
●
●
●
●
●
●
4204242043AEB610309L42729AEB910303/4AEB910305/2
Bu¥ ered Peptone Water 6 x 90 mlBPW 6 x 225 ml
BPW 6 x 900 ml bottleBPW 10 x 225 ml (mini-bags)
BPW 4 X 3L (DILUBAG™)BPW 2 X 5L (DILUBAG™)
●
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42048AEB610419L42727AEB910913/4AEB910915/2AEB140412AEB140413AEB140417
Half-Fraser 6 x 225 mlHalf-Fraser 6 x 900 ml bottle
Half-Fraser 10 x 225 ml (mini-bags)Half-Fraser 4 X 3L (DILUBAG™)Half-Fraser 2 X 5L (DILUBAG™)
Half-Fraser (base with antibiotics) 500 kgHalf-Fraser (base with antibiotics) 5 kg
Half-Fraser (base with antibiotics) 10 kg
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CHRO
MOG
ENIC
MED
IACO
NFIR
MAT
ION
BROT
HS
CONFIRMATION OF Listeria monocytogenes
for ALOA® One Day and ALOA® Count methods
For VIDAS® users: ALOA® can be used as a con rmation method of VIDAS® Listeria positive assays.
CONFIRMATION OF Listeria spp.
NEXT DAY SAME DAYALOA®ALOA®ALOA
Con� rmationAPI®
ListeriaRapidec®
ListeriaFast
RhamnoseVIDAS®
LMO2
24 hours,cost e� ective
24-houridenti� cation
positive from 4hrsnegative from 6hrs
positive from 6hrsnegative in 24hrs
70 min
SAME DAY NEXT DAYBHI Broth
+ strip Palcam
< 6 hours 24 hours,cost e� ective
DE
TECTION
E N U M E R AT I ON
ISO 16
140 VA LI DATIO N
ISO 16140VALI DATION
ENUMERATION
V A L I D A T I ON
ISO 16140
OFFICIAL
VALIDATION
AOAC VALIDATION
ISO 16
140 VA LI DATIO N
OFFICIAL
VALIDATION
AOAC VALIDATION
D E T E C T I O N
AOAC RI & ISO 16140
V A L I D A T I O N
DETECTION
V A L I D A T I ON
ISO 16140
CONFIRMATION
V A L I D A T I ON
ISO 16140
ENUMERATION
V A L I D A T I ON
ISO 16140
DETECTION
V A L I D A T I ON
AOAC RI & ISO 16140
ENUMERATION
V A L I D A T I ON
AOAC RI & ISO 16140
DETECTION
V A L I D A T I ON
AOAC RI & ISO 16140
DETECTION
E N U M E R AT I ONVALI DATI O N
ISO 16140
COMPLIANT WITH THE CE 2073/2005 REGULATION (MODIFIED BY THE 1441/2007)
2 METHODS TO FULFILL ALL YOUR LISTERIA TESTING NEEDS
X g or X ml of sample + 9X ml of 1/2 Fraser
Incubation 24 ± 2 hrs at 30 °C ± 1 °C
Spread or Streak 0.1 ml on ALOA plate
Incubation 24 hrs at 37 °C ± 1 °C(Incubation during 48 hours is possible for your convenience; i.e.: week-ends)
Presumption* of Listeria spp.
Presumption* of Listeria monocytogenes
* According to AFNOR certi� cation, all positive samples have to be con� rmed, see last page
DE
TECTION
E N U M E R AT I ON
ISO 16
140 VA LI DATIO N
ISO 16140VALI DATION
ENUMERATION
V A L I D A T I ON
ISO 16140
OFFICIAL
VALIDATION
AOAC VALIDATION
ISO 16
140 VA LI DATIO N
OFFICIAL
VALIDATION
AOAC VALIDATION
D E T E C T I O N
AOAC RI & ISO 16140
V A L I D A T I O N
DETECTION
V A L I D A T I ON
ISO 16140
CONFIRMATION
V A L I D A T I ON
ISO 16140
ENUMERATION
V A L I D A T I ON
ISO 16140
DETECTION
V A L I D A T I ON
AOAC RI & ISO 16140
ENUMERATION
V A L I D A T I ON
AOAC RI & ISO 16140
DETECTION
V A L I D A T I ON
AOAC RI & ISO 16140
DETECTION
E N U M E R AT I ONVALI DATI O N
ISO 16140
COMPLIANT WITH THE CE 2073/2005 REGULATION (MODIFIED BY THE 1441/2007)
ALOA® One DayDetection of Listeria monocytogenes and Listeria spp. within 48 hours
• Short protocol ALOA® One Day for food and environmental samples ISO 16140 and AOAC RI validated
• One day results after enrichment for negative and con� rmed positive samples
• Easy protocol: 1 bag - 1 plate
• Flexibility & work ̈ow optimization: broth bags can be held at +2 to +8°C for up to 72 hours after incubation and plates for up to 48 hours
• Simultaneous detection of Listeria spp. and Listeria monocytogenes on the same plate
ALOA® CountEnumeration of Listeria monocytogenes and Listeria spp. within 48 hours
• Short protocol ALOA® Count for food and environmental samples ISO 16140 validated
• Time to result: 48 hours on ALOA plate
• Easy protocol: 1 plate per dilution
• Flexible and cost-e� ective: - Save one hour: optional resuscitation step - Optimize work� ow: resuscitation in bu� ered peptone
water or half fraser let you carry out detection & enumeration from the same suspension
- Plates can be held at +2 to +8°C for up to 72 hours after incubation
• Di� erent possibilities for low numbers <10 CFU/g: - 1 plate Ø90 by pour plate method - 1 plate Ø140 by spreading method - 3 plates Ø90 by spreading method
X g or X ml of sample + 9X ml of 1/2 Fraser or BPW
Optional resuscitation step1 hr ± 5 min at 20 °C ± 2 °C
Presumption* of Listeria monocytogenes
Presumption* of Listeria spp.
* According to AFNOR certi� cation, all positive samples have to be con� rmed, see last page
Incubation 37 ± 1°C 48 ± 3 hrs
< 10 CFU/g Pour 1 ml in about 15 ml agar cooled at 47 ±2 °C
< 100 CFU/g Spread 0.1ml on 1 plate Ø90mm
< 10 CFU/g Spread 1 ml on 3 plates Ø90mm Spread 1 ml on 1 plate Ø140mm
DE
TECTION
E N U M E R AT I ON
ISO 16
140 VA LI DATIO N
ISO 16140VALI DATION
ENUMERATION
V A L I D A T I ON
ISO 16140
OFFICIAL
VALIDATION
AOAC VALIDATION
ISO 16
140 VA LI DATIO N
OFFICIAL
VALIDATION
AOAC VALIDATION
D E
AOAC RI
DETECTION
V A L I D A T I ON
ISO 16140
CONFIRMATION
V A L I D A T I ON
ISO 16140
ENUMERATION
V A L I D A T I ON
ISO 16140
DETECTION
V A L I D A T I ON
AOAC RI & ISO 16140
ENUMERA
V A L I D
AOAC RI &
DETECTION
V A L I D A T I O
AOAC RI & ISO 16140
DETECTION
E N U M E R AT I ONVALI DATI O N
ISO 16140
COMPLIANT WITH THE CE 2073/2005 REGULATION(MODIFIED BY THE 1441/2007)
ALOA® MEDIUM IS COMPLIANT WITH ISO 11290 HORIZONTAL METHOD FOR THE DETECTION AND ENUMERATION OF LISTERIA MONOCYTOGENES*
UN
IQU
E M
EDIA
bioMérieux S.A. • 69280 Marcy l’Étoile • France • Tel.: + 33 (0)4 78 87 20 00 • Fax: +33 (0)4 78 87 20 90www.biomerieux.com • www.biomerieux-industry.com01
-18
/930
7722
006
/GB/
B /
© b
ioM
érie
ux •
Doc
umen
t and
/or p
ictu
res
not l
egal
ly b
indi
ng. M
odifi
catio
ns b
y bi
oMér
ieux
can
be
mad
e w
ithou
t prio
r not
ice
• BI
OM
ERIE
UX, t
he B
IOM
ERIE
UX lo
go, A
PI, C
hrom
ID, D
iluba
g, R
apid
ec a
nd V
idas
are
use
d pe
ndin
g an
d/or
regi
ster
ed tr
adem
arks
bel
ongi
ng to
bi
oMér
ieux
, or o
ne o
f its
sub
sidi
arie
s or
one
of i
ts c
ompa
nies
. Any
oth
er n
ame
or tr
adem
ark
is th
e pr
oper
ty o
f its
resp
ectiv
e ow
ner.
ALOA
is a
regi
ster
ed tr
adem
ark
of B
iolif
e S.
r.l..
Accu
Prob
e is
a re
gist
red
trad
emar
k of
Hol
ogic
Inco
rpor
ated
. bi
oMér
ieux
SA
673
620
399
RCS
Lyon
/ P
hoto
s: b
ioM
érie
ux,
SHAS
HIN
KO
UBO
U H
YPER
LIN
K, N
. Bou
chut
/ P
rinte
d in
Fra
nce
/ th
éra
398
160
242
RCS
Lyon
.
ALOA®UNIQUE MEDIA
Detection, Enumeration and Con� rmation of Listeria spp. and Listeria monocytogenes
YOUR CONFIRMATION SOLUTIONS
ORDERING INFORMATION DETECTION ENUMERATIONL. mono L. spp. L. mono L. spp.
AEB520080AEB520079AEB120082AEB620088AEB150072AEB150073AEB684420AEB484204AEB120100
ALOA® 20 plates Ø 90 mmALOA® 120 plates Ø 90 mmALOA® 10 plates Ø 140 mm
ALOA® Count 5 x 180 ml (base + suppl)ALOA® BASE 500 g
ALOA® BASE 5 kgALOA® SUPPLEMENT 6 x suppl 500 ml base
ALOA® SUPPLEMENT 4 x suppl 4L baseALOA® Con� rmation 10 plates Ø 90 mm
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●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
●
037001030030704415078
Rapidec® Lmono (20 tests)API® Listeria
VIDAS® LMO2Fast Rhamnose (30 tests)
●
●
●
●
●
●
●
●
●
●
AEB110101416477AEB522050
BHI 20 tubes 1 mlListeria spp con� rmation strips
PALCAM 20 plates Ø 90 mm
●
●
●
●
●
●
4204242043AEB610309L42729AEB910303/4AEB910305/2
Bu¥ ered Peptone Water 6 x 90 mlBPW 6 x 225 ml
BPW 6 x 900 ml bottleBPW 10 x 225 ml (mini-bags)
BPW 4 X 3L (DILUBAG™)BPW 2 X 5L (DILUBAG™)
●
●
●
●
●
●
●
●
●
●
●
●
42048AEB610419L42727AEB910913/4AEB910915/2AEB140412AEB140413AEB140417
Half-Fraser 6 x 225 mlHalf-Fraser 6 x 900 ml bottle
Half-Fraser 10 x 225 ml (mini-bags)Half-Fraser 4 X 3L (DILUBAG™)Half-Fraser 2 X 5L (DILUBAG™)
Half-Fraser (base with antibiotics) 500 kgHalf-Fraser (base with antibiotics) 5 kg
Half-Fraser (base with antibiotics) 10 kg
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CHRO
MOG
ENIC
MED
IACO
NFIR
MAT
ION
BROT
HS
CONFIRMATION OF Listeria monocytogenes
for ALOA® One Day and ALOA® Count methods
For VIDAS® users: ALOA® can be used as a con rmation method of VIDAS® Listeria positive assays.
CONFIRMATION OF Listeria spp.
NEXT DAY SAME DAYALOA®ALOA®ALOA
Con� rmationAPI®
ListeriaRapidec®
ListeriaFast
RhamnoseVIDAS®
LMO2
24 hours,cost e� ective
24-houridenti� cation
positive from 4hrsnegative from 6hrs
positive from 6hrsnegative in 24hrs
70 min
SAME DAY NEXT DAYBHI Broth
+ strip Palcam
< 6 hours 24 hours,cost e� ective
DE
TECTION
E N U M E R AT I ON
ISO 16
140 VA LI DATIO N
ISO 16140VALI DATION
ENUMERATION
V A L I D A T I ON
ISO 16140
OFFICIAL
VALIDATION
AOAC VALIDATION
ISO 16
140 VA LI DATIO N
OFFICIAL
VALIDATION
AOAC VALIDATION
D E T E C T I O N
AOAC RI & ISO 16140
V A L I D A T I O N
DETECTION
V A L I D A T I ON
ISO 16140
CONFIRMATION
V A L I D A T I ON
ISO 16140
ENUMERATION
V A L I D A T I ON
ISO 16140
DETECTION
V A L I D A T I ON
AOAC RI & ISO 16140
ENUMERATION
V A L I D A T I ON
AOAC RI & ISO 16140
DETECTION
V A L I D A T I ON
AOAC RI & ISO 16140
DETECTION
E N U M E R AT I ONVALI DATI O N
ISO 16140
COMPLIANT WITH THE CE 2073/2005 REGULATION (MODIFIED BY THE 1441/2007)