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SALMA®UNIQUE MEDIA

Detection and Confirmation of Salmonella spp.

ORDERING INFORMATION

SALMA®

bioMérieux S.A. • 69280 Marcy l’Étoile • France • Tel.: + 33 (0)4 78 87 20 00 • Fax: +33 (0)4 78 87 20 90 www.biomerieux.com • www.biomerieux-industry.com

FOOD SAFETY

DECIDEWITHCONFIDENCE

SALMA®

4704690 418246418247

SALMA® One Day Kit, 120 testsSALMA® 120 plates Ø 90 mmSALMA® 20 plates Ø 90 mm

SALMONELLA SUPPLEMENT421202421203

Salmonella Supp Tab 25gSalmonella Supp Tab 375g

CONFIRMATIONMGNF42AEB526760

Salmonella spp. latex agglutination test, 50 testsSALSA™ 20 bi-plates Ø 90 mm

BUFFERED PEPTONE WATER

4204242043AEB610309LAEB910303/4AEB910305/242729421121421120421122

6 bottles 90 ml6 bottles 225 ml6 bottles 900 mlDILUBAG™ BPW 4 X 3LDILUBAG™ BPW 2 X 5LMiniBag BPW 10 x 225 mlBuffered Peptone Water 500 gBuffered Peptone Water 5 kgBuffered Peptone Water 10 kg

Please contact us for more detailed information

NB FOOD CULT-SALMA.indd 2-3 08/03/2018 12:17

Compliant with the CE 2073/2005 regulation

(modified by the 1441/2007)

DE

TECTION

E N U M E R AT I ON

ISO 16

140 VA LI DATIO N

ISO 16140VALI DATION

ENUMERATION

V A L I D A T I ON

ISO 16140

OFFICIAL

VALIDATION

AOAC VALIDATION

ISO 16

140 VA LI DATIO N

OFFICIAL

VALIDATION

AOAC VALIDATION

D E T E C T I O N

AOAC RI & ISO 16140

V A L I D A T I O N

DETECTION

V A L I D A T I ON

ISO 16140

CONFIRMATION

V A L I D A T I ON

ISO 16140

ENUMERATION

V A L I D A T I ON

ISO 16140

DETECTION

V A L I D A T I ON

AOAC RI & ISO 16140

ENUMERATION

V A L I D A T I ON

AOAC RI & ISO 16140

DETECTION

V A L I D A T I ON

AOAC RI & ISO 16140

DETECTION

E N U M E R AT I ONVALI DATI O N

ISO 16140

SALMA®Unique and innovative method• Easy, Flexible and innovative supplementation• Detection of motile and non motile Salmonella, lactose+ Salmonella and other atypical serotypes within 48hrs (enrichment included)

• ISO 16140 validation for food stuffs, animal feeding stuffs and environmental samples with no restrictions of use

Salmonella spp.: pink to purple coloniesOther Enterobacteriaceae: blue or white colonies

24 hour protocolSALSA™

Rapid protocol Agglutination latex test

C8 esterase+ strain(pink to purple

colonies)

Lactose+(yellow colonies and H2S-)

Slow C8esterase strain

LDC+ and H2S+(red colonies with black centre)

LDC+ and H2S+(red colonies with black centre)

C8 esterase+ strain(pink to purple

colonies)

High selectivity and specificity• Mix of chromogenic susbtrates targeting the esterase activities of Salmonella

• High differentiation between Salmonella and the other enterobacteria• Absence of Salmonella spp.: blue or white colonies• Presence of Salmonella spp.: pink to purple typical colonies• High inhibition of the interfering flora thanks to the combination

of an enrichment selective supplement and a highly selective chromogenic culture medium

Benefits• Simple and convenient protocol: 1 bag/1 plate method with easy

supplementation• Easy to read thanks to the colors contrast: pink to purple

colonies on white agar • Flexibility and workflow optimization: Enrichment bags and

plates can be held up to 72 hours in refrigerated conditions• Traceability of the supplementation by color change of the BPW

Simple and rapid confimation2 validated solutions are available: • Rapid Protocol: results within a few minutes thanks to

an agglutination latex test • 24 hour protocol: isolation on SALSA™ plate (ASAP™/XLD) and

incubation 24 ±2hrs at 37 ±1°C

YOUR SOLUTION FOR SALMONELLA DETECTIONHighly selective method for the detection of motile and non motile Salmonella spp. in food and environmental samples

UN

IQU

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EDIA

X g or X ml of sample in 9X ml of BPW

Absence of Salmonella spp.(no colonies, blue or white colonies)

Presence of Salmonella spp.(typical colonies: pink to purple)

Incubation 16 to 24h at 41.5±1°C

CONFIRMATION

OR

Streak 10µL on SALMA® plate

Incubation 21 to 27h at 37±1°C

Add Salmonella Supp Tab

D0

Enrichment

D1

Isolation

D2

Reading and result

BPM

BPW + Salmonella Supp Tab SALMA

RVS & MKTT XLD & ASAP Confirmation test Nutrient ISO agar+ biochemical galleriesISO 6579

1st enrichment

SINGLE enrichment +Latex confirmation

2nd enrichment

Screening in ONLY 2 days!(including confirmation)

Cost & time saving method ➤ 3 days saved !

Screening in 5 days

D1 D2 D3 D5

WORKFLOW COMPARISON: SALMA ONE DAY VS. ISO 6579

SALMA®

NB FOOD CULT-SALMA.indd 4-5 08/03/2018 12:17

bioMérieux S.A. • 69280 Marcy l’Étoile • France • Tel.: + 33 (0)4 78 87 20 00 • Fax: +33 (0)4 78 87 20 90www.biomerieux.com • www.biomerieux-industry.com01

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ALOA®UNIQUE MEDIA

Detection, Enumeration and Con� rmation of Listeria spp. and Listeria monocytogenes

YOUR CONFIRMATION SOLUTIONS

ORDERING INFORMATION DETECTION ENUMERATIONL. mono L. spp. L. mono L. spp.

AEB520080AEB520079AEB120082AEB620088AEB150072AEB150073AEB684420AEB484204AEB120100

ALOA® 20 plates Ø 90 mmALOA® 120 plates Ø 90 mmALOA® 10 plates Ø 140 mm

ALOA® Count 5 x 180 ml (base + suppl)ALOA® BASE 500 g

ALOA® BASE 5 kgALOA® SUPPLEMENT 6 x suppl 500 ml base

ALOA® SUPPLEMENT 4 x suppl 4L baseALOA® Con� rmation 10 plates Ø 90 mm

●

●

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037001030030704415078

Rapidec® Lmono (20 tests)API® Listeria

VIDAS® LMO2Fast Rhamnose (30 tests)

●

●

●

●

●

●

●

●

●

●

AEB110101416477AEB522050

BHI 20 tubes 1 mlListeria spp con� rmation strips

PALCAM 20 plates Ø 90 mm

●

●

●

●

●

●

4204242043AEB610309L42729AEB910303/4AEB910305/2

Bu¥ ered Peptone Water 6 x 90 mlBPW 6 x 225 ml

BPW 6 x 900 ml bottleBPW 10 x 225 ml (mini-bags)

BPW 4 X 3L (DILUBAG™)BPW 2 X 5L (DILUBAG™)

●

●

●

●

●

●

●

●

●

●

●

●

42048AEB610419L42727AEB910913/4AEB910915/2AEB140412AEB140413AEB140417

Half-Fraser 6 x 225 mlHalf-Fraser 6 x 900 ml bottle

Half-Fraser 10 x 225 ml (mini-bags)Half-Fraser 4 X 3L (DILUBAG™)Half-Fraser 2 X 5L (DILUBAG™)

Half-Fraser (base with antibiotics) 500 kgHalf-Fraser (base with antibiotics) 5 kg

Half-Fraser (base with antibiotics) 10 kg

●

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CHRO

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MED

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NFIR

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BROT

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CONFIRMATION OF Listeria monocytogenes

for ALOA® One Day and ALOA® Count methods

For VIDAS® users: ALOA® can be used as a con rmation method of VIDAS® Listeria positive assays.

CONFIRMATION OF Listeria spp.

NEXT DAY SAME DAYALOA®ALOA®ALOA

Con� rmationAPI®

ListeriaRapidec®

ListeriaFast

RhamnoseVIDAS®

LMO2

24 hours,cost e� ective

24-houridenti� cation

positive from 4hrsnegative from 6hrs

positive from 6hrsnegative in 24hrs

70 min

SAME DAY NEXT DAYBHI Broth

+ strip Palcam

< 6 hours 24 hours,cost e� ective

DE

TECTION

E N U M E R AT I ON

ISO 16

140 VA LI DATIO N

ISO 16140VALI DATION

ENUMERATION

V A L I D A T I ON

ISO 16140

OFFICIAL

VALIDATION

AOAC VALIDATION

ISO 16

140 VA LI DATIO N

OFFICIAL

VALIDATION

AOAC VALIDATION

D E T E C T I O N

AOAC RI & ISO 16140

V A L I D A T I O N

DETECTION

V A L I D A T I ON

ISO 16140

CONFIRMATION

V A L I D A T I ON

ISO 16140

ENUMERATION

V A L I D A T I ON

ISO 16140

DETECTION

V A L I D A T I ON

AOAC RI & ISO 16140

ENUMERATION

V A L I D A T I ON

AOAC RI & ISO 16140

DETECTION

V A L I D A T I ON

AOAC RI & ISO 16140

DETECTION

E N U M E R AT I ONVALI DATI O N

ISO 16140

COMPLIANT WITH THE CE 2073/2005 REGULATION (MODIFIED BY THE 1441/2007)

2 METHODS TO FULFILL ALL YOUR LISTERIA TESTING NEEDS

X g or X ml of sample + 9X ml of 1/2 Fraser

Incubation 24 ± 2 hrs at 30 °C ± 1 °C

Spread or Streak 0.1 ml on ALOA plate

Incubation 24 hrs at 37 °C ± 1 °C(Incubation during 48 hours is possible for your convenience; i.e.: week-ends)

Presumption* of Listeria spp.

Presumption* of Listeria monocytogenes

* According to AFNOR certi� cation, all positive samples have to be con� rmed, see last page

DE

TECTION

E N U M E R AT I ON

ISO 16

140 VA LI DATIO N

ISO 16140VALI DATION

ENUMERATION

V A L I D A T I ON

ISO 16140

OFFICIAL

VALIDATION

AOAC VALIDATION

ISO 16

140 VA LI DATIO N

OFFICIAL

VALIDATION

AOAC VALIDATION

D E T E C T I O N

AOAC RI & ISO 16140

V A L I D A T I O N

DETECTION

V A L I D A T I ON

ISO 16140

CONFIRMATION

V A L I D A T I ON

ISO 16140

ENUMERATION

V A L I D A T I ON

ISO 16140

DETECTION

V A L I D A T I ON

AOAC RI & ISO 16140

ENUMERATION

V A L I D A T I ON

AOAC RI & ISO 16140

DETECTION

V A L I D A T I ON

AOAC RI & ISO 16140

DETECTION

E N U M E R AT I ONVALI DATI O N

ISO 16140

COMPLIANT WITH THE CE 2073/2005 REGULATION (MODIFIED BY THE 1441/2007)

ALOA® One DayDetection of Listeria monocytogenes and Listeria spp. within 48 hours

• Short protocol ALOA® One Day for food and environmental samples ISO 16140 and AOAC RI validated

• One day results after enrichment for negative and con� rmed positive samples

• Easy protocol: 1 bag - 1 plate

• Flexibility & work ̈ow optimization: broth bags can be held at +2 to +8°C for up to 72 hours after incubation and plates for up to 48 hours

• Simultaneous detection of Listeria spp. and Listeria monocytogenes on the same plate

ALOA® CountEnumeration of Listeria monocytogenes and Listeria spp. within 48 hours

• Short protocol ALOA® Count for food and environmental samples ISO 16140 validated

• Time to result: 48 hours on ALOA plate

• Easy protocol: 1 plate per dilution

• Flexible and cost-e� ective: - Save one hour: optional resuscitation step - Optimize work� ow: resuscitation in bu� ered peptone

water or half fraser let you carry out detection & enumeration from the same suspension

- Plates can be held at +2 to +8°C for up to 72 hours after incubation

• Di� erent possibilities for low numbers <10 CFU/g: - 1 plate Ø90 by pour plate method - 1 plate Ø140 by spreading method - 3 plates Ø90 by spreading method

X g or X ml of sample + 9X ml of 1/2 Fraser or BPW

Optional resuscitation step1 hr ± 5 min at 20 °C ± 2 °C

Presumption* of Listeria monocytogenes

Presumption* of Listeria spp.

* According to AFNOR certi� cation, all positive samples have to be con� rmed, see last page

Incubation 37 ± 1°C 48 ± 3 hrs

< 10 CFU/g Pour 1 ml in about 15 ml agar cooled at 47 ±2 °C

< 100 CFU/g Spread 0.1ml on 1 plate Ø90mm

< 10 CFU/g Spread 1 ml on 3 plates Ø90mm Spread 1 ml on 1 plate Ø140mm

DE

TECTION

E N U M E R AT I ON

ISO 16

140 VA LI DATIO N

ISO 16140VALI DATION

ENUMERATION

V A L I D A T I ON

ISO 16140

OFFICIAL

VALIDATION

AOAC VALIDATION

ISO 16

140 VA LI DATIO N

OFFICIAL

VALIDATION

AOAC VALIDATION

D E

AOAC RI

DETECTION

V A L I D A T I ON

ISO 16140

CONFIRMATION

V A L I D A T I ON

ISO 16140

ENUMERATION

V A L I D A T I ON

ISO 16140

DETECTION

V A L I D A T I ON

AOAC RI & ISO 16140

ENUMERA

V A L I D

AOAC RI &

DETECTION

V A L I D A T I O

AOAC RI & ISO 16140

DETECTION

E N U M E R AT I ONVALI DATI O N

ISO 16140

COMPLIANT WITH THE CE 2073/2005 REGULATION(MODIFIED BY THE 1441/2007)

ALOA® MEDIUM IS COMPLIANT WITH ISO 11290 HORIZONTAL METHOD FOR THE DETECTION AND ENUMERATION OF LISTERIA MONOCYTOGENES*

UN

IQU

E M

EDIA

bioMérieux S.A. • 69280 Marcy l’Étoile • France • Tel.: + 33 (0)4 78 87 20 00 • Fax: +33 (0)4 78 87 20 90www.biomerieux.com • www.biomerieux-industry.com01

-18

/930

7722

006

/GB/

B /

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/ P

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Fra

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398

160

242

RCS

Lyon

.

ALOA®UNIQUE MEDIA

Detection, Enumeration and Con� rmation of Listeria spp. and Listeria monocytogenes

YOUR CONFIRMATION SOLUTIONS

ORDERING INFORMATION DETECTION ENUMERATIONL. mono L. spp. L. mono L. spp.

AEB520080AEB520079AEB120082AEB620088AEB150072AEB150073AEB684420AEB484204AEB120100

ALOA® 20 plates Ø 90 mmALOA® 120 plates Ø 90 mmALOA® 10 plates Ø 140 mm

ALOA® Count 5 x 180 ml (base + suppl)ALOA® BASE 500 g

ALOA® BASE 5 kgALOA® SUPPLEMENT 6 x suppl 500 ml base

ALOA® SUPPLEMENT 4 x suppl 4L baseALOA® Con� rmation 10 plates Ø 90 mm

●

●

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●

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●

●

●

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●

●

●

●

●

●

●

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●

●

●

037001030030704415078

Rapidec® Lmono (20 tests)API® Listeria

VIDAS® LMO2Fast Rhamnose (30 tests)

●

●

●

●

●

●

●

●

●

●

AEB110101416477AEB522050

BHI 20 tubes 1 mlListeria spp con� rmation strips

PALCAM 20 plates Ø 90 mm

●

●

●

●

●

●

4204242043AEB610309L42729AEB910303/4AEB910305/2

Bu¥ ered Peptone Water 6 x 90 mlBPW 6 x 225 ml

BPW 6 x 900 ml bottleBPW 10 x 225 ml (mini-bags)

BPW 4 X 3L (DILUBAG™)BPW 2 X 5L (DILUBAG™)

●

●

●

●

●

●

●

●

●

●

●

●

42048AEB610419L42727AEB910913/4AEB910915/2AEB140412AEB140413AEB140417

Half-Fraser 6 x 225 mlHalf-Fraser 6 x 900 ml bottle

Half-Fraser 10 x 225 ml (mini-bags)Half-Fraser 4 X 3L (DILUBAG™)Half-Fraser 2 X 5L (DILUBAG™)

Half-Fraser (base with antibiotics) 500 kgHalf-Fraser (base with antibiotics) 5 kg

Half-Fraser (base with antibiotics) 10 kg

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CHRO

MOG

ENIC

MED

IACO

NFIR

MAT

ION

BROT

HS

CONFIRMATION OF Listeria monocytogenes

for ALOA® One Day and ALOA® Count methods

For VIDAS® users: ALOA® can be used as a con rmation method of VIDAS® Listeria positive assays.

CONFIRMATION OF Listeria spp.

NEXT DAY SAME DAYALOA®ALOA®ALOA

Con� rmationAPI®

ListeriaRapidec®

ListeriaFast

RhamnoseVIDAS®

LMO2

24 hours,cost e� ective

24-houridenti� cation

positive from 4hrsnegative from 6hrs

positive from 6hrsnegative in 24hrs

70 min

SAME DAY NEXT DAYBHI Broth

+ strip Palcam

< 6 hours 24 hours,cost e� ective

DE

TECTION

E N U M E R AT I ON

ISO 16

140 VA LI DATIO N

ISO 16140VALI DATION

ENUMERATION

V A L I D A T I ON

ISO 16140

OFFICIAL

VALIDATION

AOAC VALIDATION

ISO 16

140 VA LI DATIO N

OFFICIAL

VALIDATION

AOAC VALIDATION

D E T E C T I O N

AOAC RI & ISO 16140

V A L I D A T I O N

DETECTION

V A L I D A T I ON

ISO 16140

CONFIRMATION

V A L I D A T I ON

ISO 16140

ENUMERATION

V A L I D A T I ON

ISO 16140

DETECTION

V A L I D A T I ON

AOAC RI & ISO 16140

ENUMERATION

V A L I D A T I ON

AOAC RI & ISO 16140

DETECTION

V A L I D A T I ON

AOAC RI & ISO 16140

DETECTION

E N U M E R AT I ONVALI DATI O N

ISO 16140

COMPLIANT WITH THE CE 2073/2005 REGULATION (MODIFIED BY THE 1441/2007)