enzymes and industry
TRANSCRIPT
PRESENTED BY:Anum YousafAtia Gulzar
Ayesha ArshadFatima Muhammad
Kainat ZahraNida Javaid
Nosheen Rehman
PRESENTED TO:Dr.zehra Agha
DATE OF PRESENTATIONAPRIL 28TH, 2015
Plant sources
Animal sources
Microbial sources
Papain
Bromoline
Fixin
maltase
Trypsin
Pepsin
Lipase
rennet
Amylases
diastases
Enzymes in food industry:
• Enzymes have been having an impact on our food supply for 1000 of years.
• Use of enzymes became an offshoot of a biological or microbiological discovery that involved fermented foods.
Brewing and alcohol productionVinegarBaking etc.
Gluco-amylase: Corn syrup Light beer
Beta-glucans: Used in filtration after mashing in brewing
Lipase : Enhance flavor Cheese ripening
Some important enzymes
Chymosin: Curdling of milk in cheese making.
Microbial proteases:Used in processing of raw plants and animal protein.Used in production of meat extracts, and meat extenders.
Papain:Meat tenderizer.
Pectinase:Fruit juices
Lactase:Additive in dairy products. It is isolated from fungi “Aspergillus oryzae.”
Glucose oxidase:Convert glucose into gluconic acid.
Curing
Soaking
De-hairing
De-greasin
g
Not directly involved
Preserve Skin
Pancreatic ProteasesRemove Non Fibrillar
Protein
Neutral proteasesImprove waste water Quality
Lipases & ProteasesRemove fats
Bating
Trypsin & Proteases
To make soft and supple
TanningDirectly not
involvedQuality of tanning
Waste Processing
Trypsin & Proteolytic enzymes
Tanned waste processing
Enzymes used in pulp and paper industry Amylase ---starch Cellulase ---cellulose fiber Protease ---proteins Hemicellulases (Xylanase) ---
hemicellulose Lipase ---glycerol backbone,
pitch Esterase ---esters, stickies Pectinase ---pectins
Restriction endonucleases can be used to construct a restriction map of a DNA molecule
• Endonuclease III: Radiation, oxidation, and UV damage
• Endonuclease IV: Oxidizing agents (bleomycin, tert-butyl hydroperoxide), alkylating agents (MMS, mitomycin)
• Endonuclease V : Recognizes cis-syn and trans-syn cyclobutane pyrimidine dimers and AP sites with equal efficiency. The enzyme cleaves AP sites on both double-, and single-stranded DNA.