experiment questions enzyme temperature. state one factor that you kept constant during the...
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![Page 1: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/1.jpg)
Experiment Questions
Enzyme Temperature
![Page 2: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/2.jpg)
Enzyme Temperature
State one factor that you kept constant during the investigation.
pH or substrate conc. or enzyme conc.
![Page 3: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/3.jpg)
Enzyme Temperature
How did you vary the temperature?
Water baths (at different temps.)
![Page 4: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/4.jpg)
Enzyme Temperature
How did you know that the enzyme had completed its activity?
No more product/ Colour change/ No more bubbles/ No more foam
![Page 5: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/5.jpg)
Enzyme Temperature
Name the enzyme that you used.
Name of enzyme, e.g. catalase
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Enzyme Temperature
Name the substrate of the enzyme that you used.
Name of matching substrate, e.g. hydrogen peroxide
![Page 7: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/7.jpg)
Enzyme Temperature
A number of factors were kept constant during this experiment. Explain how you kept one of
them constant?
pH—used a buffersubstrate conc.—same volumeenzyme conc.—same amount
![Page 8: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/8.jpg)
Enzyme Temperature
How did you measure the rate of activity of the enzyme?
Time / change e.g. colour, foam, etcor data logger / sensor named
![Page 9: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/9.jpg)
Enzyme Temperature
What was the result of your investigation?
Activity varies with temperature or reference to activity at a particular temperature
![Page 10: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/10.jpg)
Enzyme Temperature
(i) Name an enzyme that you used.(ii) Name the substance that this enzyme acted
on.
(i) Catalase or amylase (ii) H2O2 or starch (must match enzyme)
![Page 11: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/11.jpg)
Enzyme Temperature
How did you vary the temperature?
Water baths/ at different temperatures/ thermostat
![Page 12: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/12.jpg)
Enzyme Temperature
How did you measure the rate of the enzyme’s action?
Volumes of foam/ per minute/ disappearance of blue colour/ per minute
![Page 13: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/13.jpg)
Enzyme Temperature
(i) What enzyme did you use? (ii) What substrate did you use?
Name of (i) enzyme, e.g. catalase (ii) substrate, e.g. H2O2 —must match
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Enzyme Temperature
How did you know that the enzyme had completed its activity?
No more product /colour change / no more bubbles / no more foam
![Page 15: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/15.jpg)
Enzyme Temperature
How did you vary the temperature in your experiment?
Water bath / different temperature treatments / Bunsen / thermostat
![Page 16: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/16.jpg)
Enzyme Temperature
(i) Name the enzyme that you used.(ii) Name the substrate with which the enzyme
reacts.
(i) Name of enzyme (ii) Name of substrate [must match enzyme]
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Enzyme Temperature
How did you vary the temperature?
Water bath or ice or thermostat
![Page 18: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/18.jpg)
Enzyme Temperature
How did you keep a constant pH during the investigation?
Buffer
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Enzyme Temperature
How did you measure the rate of activity of the enzyme?
Time / change (colour, height of foam, etc.) OR sensor / data logger
![Page 20: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/20.jpg)
Enzyme Temperature
What was the result of your investigation?
Activity increases or enzyme has an optimum or graph showing increase
![Page 21: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/21.jpg)
Enzyme Temperature
Name the enzyme that you used.Name the substrate of this enzyme.
e.g. Enzyme – catalase; Substrate - Hydrogen peroxide
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Enzyme Temperature
Why was it necessary to keep the pH constant in the course of the investigation?
To eliminate it as a possible influence on rate or only one variable
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Enzyme Temperature
How did you keep the pH constant?
Buffer
![Page 24: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/24.jpg)
Enzyme Temperature
How did you vary the temperature in the course of the investigation?
Water baths or water bath at different temperatures
![Page 25: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/25.jpg)
Enzyme Temperature
How did you know that the enzyme was working?
Description of visible result matching enzyme (or substrate)
![Page 26: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/26.jpg)
Enzyme Temperature
What enzyme did you use? What substrate did you use?
e.g. Enzyme – CatalaseSubstrate – Hydrogen peroxide (Must match
enzyme)
![Page 27: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/27.jpg)
Enzyme Temperature
How did you vary the temperature during the investigation?
Water bath
![Page 28: Experiment Questions Enzyme Temperature. State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc](https://reader036.vdocument.in/reader036/viewer/2022062308/56649dba5503460f94aaa772/html5/thumbnails/28.jpg)
Enzyme Temperature
How did you measure the rate of enzyme activity?
e.g. Volume of froth / time (Depends on enzyme used)
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Enzyme Temperature
During this investigation pH was kept constant. How did you keep the pH constant?
Buffer
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Enzyme Temperature
What was the result of your investigation?
Increasing activity with increasing temp / works best at certain temp / activity decreases above or below certain temp. (Can be shown
by graph with one axis labelled)