Fast 384-Well Thermal Cycling on the Applied Biosystems 7900HT Fast Real-Time PCR System

Product Bulletin Fast 384-Well Thermal Cycling

www.appliedbiosystems.com

• Fast 384-well real-time PCRcycling in

required. Applied Biosystems 7900HTFast Real-Time PCR Systems arealready equipped with this new powersupply. For ABI PRISM® 7900HTSequence Detection Systems, purchasedbefore October 2004, AppliedBiosystems will provide an affordableservice upgrade (P/N 4351412 or4362078) to accommodate fast thermalcycling runs. Note that upgrades andblocks are sold separately.

Proven PerformanceThe fast 384-well system offers thesame high-quality performance youhave come to expect from all otherblock formats and thermal cyclingmodes on the 7900HT system.Additionally, users will also receive thebenefit of a 50% reduction in time-to-results in a 384-well format(Table 1 and Figures 1 and 2).

www.appliedbiosystems.com

Figure 2. Amplification using human genomic DNA template demonstrating the detection of ten copies of theRNase P gene. Samples were run in 32 replicates using the fluoregenic 5' nuclease TaqMan® assay.

ΔR

n

0 5 10 15 20 25 30 35 40

Cycle

Amplification Plot1.000 E+1

1.000

1.000 E–1

ΔR

N

0 5 10 15 20 25 30 35 40

Cycle

Amplification Plot1.000 E+1

1.000

1.000 E–1

Figure 1. Amplification plot, using the 384-well fast thermal cycling protocol on the 7900HT system. Results illustrate 9 logs of linear dynamic range for 18S rRNA transcript inten-fold serial dilutions (500 ng-0.5 fg) utilizing TaqMan® Fast Universal Master Mix in 16 replicates.

CT

Standard Plot

39

37

35

33

31

29

27

25

23

21

19

17

15

13

11

9

7

5

30.0 1.0 E-6 1.0 E-5 1.0 E-4 1.0 E-3 1.0 E-2 1.0 E-1 1.0 1.0 E+1 1.0 E+2

Quantity

Standard Curve

Slope: -3.6095462Y-Inter: 12.772669

R2: 0.99948007

www.appliedbiosystems.com

Figure 3. PCR amplification plate demonstrating uniform results across the entire 384-well plate, running on fast thermal cycling conditions. A) Singleplex reaction of the humanRNase P gene with FAM™ dye-labeled TaqMan® assay. B) Multiplex TaqMan® assays of the TGFbeta (VIC® dye-labeled) and 18S rRNA (FAM dye-labeled) human transcripts.

ΔR

n

0 5 10 15 20 25 30 35 40

Cycle

A) Amplification Plot

1.000

1.000 E–1

ΔR

n

0 5 10 15 20 25 30 35 40

Cycle

B) Amplification Plot1.000 E+1

1.000

PrecisionPrecise and accurate data is an essentialcomponent for success in real-timePCR. Applied Biosystems 7900HTFast Real-Time PCR System users,performing fast 384-well runs, will beable to obtain impressively uniformresults across the entire plate, for bothsingleplex or multiplex quantitativeassays (Figure 3).

ConclusionThe new fast 384-well capabilityexpands the flexibility of the Applied Biosystems 7900HT FastReal-Time PCR System, furtherenabling the instrument to meet the changing requirements of the scientific community.

The Gold Standard in Real-Time PCRThe 7900HT system combined with TaqMan® Genomic Assays, acknowledged asthe gold standard in real-time PCR, enables you to achieve unprecedentedthroughput and flexibility, allowing you to pursue projects beyond the scope ofprevious real-time instruments. Researcher-friendly software, together with theentire suite of convenient, off-the-shelf or custom gene expression and SNPgenotyping assays, makes it easy for labs of all sizes to realize the full potential ofthis powerful research tool.

For more information visit www.7900HT.com.

For Research Use Only. Not for use in diagnostic procedures.

©2006 Applied Biosystems. All Rights Reserved. ABI PRISM, Applied Biosystems, AB (Design), MicroAmp and VIC are registered trademarks and Applera and FAM are trademarks of AppleraCorporation or its subsidiaries in the U.S. and/or certain other countries. TaqMan is a registered trademark of Roche Molecular Systems, Inc. Certain Applied Biosystems reagents are devel-oped by patents owned by Roche Molecular Systems, Inc. and F Hoffmann-La Roche, Ltd. This instrument is an Authorized Thermal Cycler under the PCR process patents. Its purchase includesno rights under the 5’ nuclease process patents.

Printed in the USA. 05/2006 Publication 117PB21-02

International SalesFor our office locations please call the divisionheadquarters or refer to our Web site atwww.appliedbiosystems.com/about/offices.cfm

Headquarters850 Lincoln Centre Drive | Foster City, CA 94404 USAPhone 650.638.5800 | Toll Free 800.345.5224www.appliedbiosystems.com

Product LiteratureFor more information about fast 384-well thermal cycling, the following User Bulletincan be downloaded from the Applied Biosystems Web site: Performing Fast GeneQuantitation with 384-Well Plates (P/N 4369584).

Ordering Information

Instrument Systems P/N

Applied Biosystems 7900HT Fast Real-Time PCR System with 4329002384-Well Block Module and Automation Accessory (200-240V)

Applied Biosystems 7900HT Fast Real-Time PCR System with 4329001384-Well Block Module (200-240V)

Applied Biosystems 7900HT Fast Real-Time PCR System with 432900496-Well Block Module and Automation Accessory (200-240V)

Applied Biosystems 7900HT Fast Real-Time PCR System with 432900396-Well Block Module (200-240V)

Applied Biosystems 7900HT Fast Real-Time PCR System with 4351405Fast 96-Well Block Module (200-240V)

Accessories

7900HT System Fast Service Upgrade 4351412

7900HT System Fast Service Upgrade with Computer 4362078

TaqMan® Low Density Array Upgrade Kit 4329012

Fast 96-Well Block Upgrade Kit 4351402

384-Well Block Upgrade Kit 4331406

96-Well Block Upgrade Kit 4331405

Enterprise Edition Sequence Detection Software 4350490

Automation Accessory Upgrade (100-240V) 4329007