Elemental Scientific

FAST for prep3 enhances laboratory productivity by adding FAST uptake, washout, and high throughput capabilities to the prep3 autodilution system. Syngistix control of a 7 port valve and three independent microperistaltic pumps is used to perform accurate and precise inline autodilution. Rapid mixing of samples or standards with diluent and internal standard in a fl uoropolymer sample fl ow path provides minimal memory effects.

FAST for prep3Automated Inline Dilution System for PerkinElmer Optima

FAST for prep3 Automated Inline Dilution System with Autosampler

2prep3-87A prep3 with

2DX Autosampler

FX-57C FASTX Complete

Features

• Triple low-pulsing microperistaltic pump

- P1 = Carrier

- P2 = Diluent

- P3 = Internal standard

• FAST injection

• In-valve mixing

Benefits

• Automatic calibration from stock standard solution(s)

• Automatic sample dilution

• Automatic over-range dilution

• Automatic internal standard out-of-range dilution

• Analyze samples from undiluted to up to 25x diluted

• Fully supported by PerkinElmer Syngistix software

Ni Calibration Curve

Figure 1. Two calibration standards, 0.5 ppm and 10 ppm, were used to create the calibration curve from 0.025 ppm to 10 ppm.

Elemental Scientific

Figure 2. Defining stock standard concentration and autosampler location in the Syngistix software is performed in the calibration tab. In this example, autocalibration from two stock standards creates a calibration curve from 0.025 ppm to 10 ppm for Fe, Mg, Mn, V, and 2.5ppm to 50ppm for Ca. Syngistix uses the stock standard locations, concentrations, and dilutions to autocalibrate the Optima.

Autocalibration of multiple stock standards to create calibration curve from 0.025 ppm to 10 ppm

Figure 3. Syngistix flexibility allows autodilution factors to be the same for all samples (example A) or sample specific (example B). FAST for prep3 eliminates offline manual dilution of samples.

Automatic predefined dilution of samples to eliminate manual offline dilution of samples

A B

Elemental Scientific

Autodilution of over-range samplesfor over-range analytes

Figure 4. The upper concentration range for one or more analytes can be defined for automatic dilution of over-range samples. Set the upper limit (A) and the over-range dilution factor (B).

Figure 5. Syngistix monitors the internal standard and FAST for prep3 automatically dilutes out-of-range samples further for reanalysis.

Automatic dilution for out-of-range internal standard

B

A

Range

Dilute Further

Elemental Scientific

16099

0%20%40%60%80%

100%120%140%160%180%200%

1 11 21 31 41 51 61 71 81 91 101

111

121

131

141

151

161

171

181

191

201

211

221

% R

ecov

ery

Sample No

% Recovery – 1 ppm Y Internal Standard

Y 371.029

% Recovery - 1 ppm Y Internal Standard

Figure 6. 9+ hour internal standard recovery. Average recovery: 98.5%.

0

20

40

60

80

100

120

Al Mg Ni Co Mn Ba Be Zn B

% R

ecov

ery

Analyte

% Average Recovery For Analytes 200+Average % Recoveries for Analytes

Figure 7. Average % recovery for over 200 samples of 0.5 ppm & 10 ppm analyte concentrations. Average analyte recovery was 100.8%.

3. After the instrument’s analysis has completed, the loop is rinsed and the nebulizer line and spray chamber are washed by the carrier, diluent, and internal standard

Sample

Vacuum

1. Vaccum load sample into loop while carrier, diluent, and internal standard rinse the nebulizer line

7: IS / Diluent

2: Carrier

3: Nebulizer

2. Peristaltic pumps adjust flow rates to perform the dilution and add internal standard inline

7: IS / Diluent

2: Carrier

3: Nebulizer

Rinse

Vacuum

7: IS / Diluent

2: Carrier

3: Nebulizer

FAST for prep3 Flow Path

98.5% Internal Standard Recovery

For more information on prep3, please visit www.prep3.com or contact us at prep3@icpms.com

Elemental Scientific7277 World Communications Dr.Omaha, NE 68122 USA Phone: 1.402.991.7800

FAST for prep3(Just add a FASTX Complete to any prep3 System)

P/N DescriptionFX-57C FAST X Complete

prep3 SystemsP/N Description

prep3-87A prep3 Inline Autodilution System for existing DX autosampler user

2prep3-87A prep3 2DX Inline Autodilution System

4prep3-87A prep3 4DX Inline Autodilution System